Selective binding of carcinoembryonic antigen using imprinted polymeric hydrogels

A poly(allylamine hydrochloride) carcinoembryonic antigen-imprinted hydrogel,was synthesized using a water-soluble crosslinker, ethylene glycol diglycidyl ether, to investigate its viability for protein recognition. The imprinting factor of the imprinted hydrogel toward carcinoembryonic antigen was found to be similar to 5, while the imprinting factor of the imprinted hydrogel toward alpha-fetoprotein was determined to be similar to 2, suggesting selectivity and specificity toward the template protein. This work lays the foundation for the development of a novel line of imprinted hydrogel systems capable of protein recognition for diagnostic and therapeutic applications. (c) 2008 Wiley Periodicals, Inc.