Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 25, Issue 6, Pages 574-593Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2014.884427
Keywords
gelatin; nanofiber; PHBV; esophagus; HEEpiC
Funding
- Indian Council of Medical Research, India [35/21/2010-BMS]
- Nano Mission [SR/S5/NM-07/2006, SR/NM/PG-16/2007]
- Department of Science & Technology, India
- FIST, Department of Science & Technology, India [SR/FST/LSI-327/2007]
- Drugs & Pharmaceuticals Research Programme, Department of Science & Technology, India
- SASTRA University
- Council of Scientific and Industrial Research [09/1095/(0003)/2013/EMR-I]
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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and PHBV-gelatin were electrospun to obtain defect-free nanofibers by optimizing various process and solution parameters. Tensile strength, Young's modulus, and wettability of PHBV-gelatin nanofibrous scaffold were determined and compared with PHBV nanofibrous scaffold. Our results demonstrate that PHBV-gelatin nanofibers exhibited higher tensile strength and Young's modulus than the PHBV nanofibers. Human esophageal epithelial cells (HEEpiC) were cultured on PHBV and PHBV-gelatin nanofiber showed better cell proliferation in PHBV nanofibrous scaffold than the PHBV-gelatin scaffold after 7 days of culture. HEEpiC cultured on PHBV and PHBV-gelatin nanofibrous scaffold exhibited characteristic epithelial cobblestone morphology after 3 days of culture. Further, the HEEpiC extracellular matrix (ECM) proteins (collagen type IV and laminin) and phenotypic marker proteins (cytokeratin-4 and 14) expressions were significantly higher in PHBV-gelatin nanofibrous scaffold than the PHBV nanofiber scaffold. However, the long-term stability and functional state of the cells on the PHBV scaffold give it an edge over the blend scaffolds. Thus, PHBV-based nanofibrous scaffolds could be explored further as ECM substitutes for the regeneration of esophageal tissue.
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