Article
Biochemistry & Molecular Biology
Kinga Gostomska-Pampuch, Jacek R. Wisniewski, Karol Sowinski, Wieslaw Gruszecki, Andrzej Gamian, Magdalena Staniszewska
Summary: The study found that the glycation product MAGE, generated from the reaction of melibiose with a protein, is more efficiently formed under dry conditions compared to aqueous conditions. Different structures of myoglobin with melibiose were identified, including crosslinking and non-crosslinking modifications. The results suggest that MAGE adduct is initiated by coupling melibiose to a model myoglobin protein and can further rearrange into more advanced structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Mehar Un Nissa, Nevil Pinto, Arijit Mukherjee, Panga Jaipal Reddy, Biplab Ghosh, Zhi Sun, Saicharan Ghantasala, Chetanya Chetanya, Sanjyot Vinayak Shenoy, Robert L. Moritz, Mukunda Goswami, Sanjeeva Srivastava
Summary: Proteomic studies on Labeo rohita provided a comprehensive organ-based protein profiling, identifying a large number of proteins and global post-translational modifications. These findings offer important insights for basic and applied research in aquaculture field.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Nai-Hao Yin, Anthony W. Parker, Pavel Matousek, Helen L. Birch
Summary: The purpose of this study is to determine if Raman spectroscopy can detect age-related changes in tendon matrix molecules. The study found that old age groups had significantly higher Raman intensities and fluorescence levels compared to young tendons. Principal component analysis was able to distinguish between different age groups and tendon types. There was also a significant correlation between Raman spectra and levels of advanced glycation end-product (AGE) collagen crosslinks. These findings provide a basis for quantifying age-related chemical modifications to tendon matrix molecules in intact tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Shichen Zhang, Wei Ju, Xiaoyi Chen, Yanyan Zhao, Lingchong Feng, Zi Yin, Xiao Chen
Summary: This review summarizes recent research on the structure and function of the extracellular matrix (ECM) components of tendons and highlights the application of multiple detection methodologies concerning the structure of ECMs. The focus is on macro to micro detection methods for tendons, and current techniques for evaluating the extracellular matrix of tendons at the micro level are introduced in detail. The review also emphasizes future ECM detection methods and the potential for fabricating biomimetic tendons.
BIOACTIVE MATERIALS
(2022)
Article
Oncology
Elisa Holstein, Annalena Dittmann, Anni Kaariainen, Vilma Pesola, Jarkko Koivunen, Taina Pihlajaniemi, Alexandra Naba, Valerio Izzi
Summary: Mutations play a crucial role in the oncogenic process by altering regulatory pathways and leading to uncontrolled cell proliferation. While mutations in intracellular proteins are well understood, less is known about mutations in extracellular matrix-associated proteins. This study highlights the specificity of mutations affecting post-translational modification sites in the extracellular matrix and their potential impact on the tumor microenvironment.
Article
Biochemical Research Methods
Hauh-Jyun Candy Chen, Yan-Ling Liao
Summary: Although malondialdehyde and methylglyoxal have the same molecular formula, they have different chemistry in forming protein adducts. The major lysine adduct of malondialdehyde in hemoglobin is the N-propenal type, while that of methylglyoxal is N6-(1-carboxyethyl)lysine. This Letter provides evidence that the methylglyoxal-like hemoglobin adducts are not derived from malondialdehyde. This Letter also discusses the quantification of malondialdehyde-induced post-translational modifications in human hemoglobin by different mass spectrometry-based methods.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Cell Biology
Takenori Omoto, Dilimulati Yimiti, Yohei Sanada, Minoru Toriyama, Chenyang Ding, Yuta Hayashi, Yasunari Ikuta, Tomoyuki Nakasa, Masakazu Ishikawa, Masayuki Sano, Minjung Lee, Takayuki Akimoto, Chisa Shukunami, Shigeru Miyaki, Nobuo Adachi
Summary: This study investigated the role of DICER in tendon maturation using Dicer conditional knockout (cKO) mice and identified key miRNAs in tendon maturation. The findings suggest that DICER plays an important role in tendon development, and miR-135a may be a crucial miRNA for tendon maturation and healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Danielle Steffen, Michael J. Mienaltowski, Keith Baar
Summary: The effect of mechanical load on tendinopathic tissue varies depending on the type of load. Dynamic loading increases fibrocartilage markers, while long isometric loads stimulate markers of tendon regeneration.
Article
Oncology
Pooja Gulhane, Shailza Singh
Summary: Non-Small Cell Lung Cancer (NSCLC) is the most common type of lung cancer, accounting for about 85% of all cases. Despite the availability of numerous therapies, the survival rate is low, highlighting the need to understand the mechanisms underlying NSCLC cell survival and progression. Post Translational Modifications (PTMs), such as phosphorylation, methylation, and acetylation, have been implicated in cancer progression and metastasis, including NSCLC. A comprehensive understanding of PTMs associated with NSCLC biology can offer novel therapeutic targets and insights into current treatment approaches.
TRANSLATIONAL ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Carolina Anazco, Janin Riedelsberger, Lorenzo Vega-Montoto, Armando Rojas
Summary: Collagen is a vital structural protein in mammals and is strengthened by complex enzymatic and non-enzymatic reactions, such as covalent cross-linking and glycation. This review article explores the amine oxidase-like activity of plant polyphenols and their potential as antiglycation agents. It also discusses the molecular mechanisms by which specific flavonoids impact or protect the normal collagen cross-linking process, with the aim of promoting the stabilization of highly organized collagen fibrils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Yun Li, Hong Yang, Tianhan He, Liang Zhang, Chao Liu
Summary: Cav1.2 plays a crucial role in neurodegenerative diseases, but the regulation of its activity remains incompletely understood. The repurposing of DHP drugs may offer potential therapeutic strategies, but further research is needed.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biophysics
R. J. Leiphart, S. N. Weiss, M. S. DiStefano, A. A. Mavridis, S. A. Adams, N. A. Dyment, L. J. Soslowsky
Summary: This study investigates the role of collagen V in tendon healing using knockdown models. The results show that Col5a1 knockdown affects tendon gene expression and collagen fibril size and shape, with heterozygous knockdown resulting in improved stiffness of healing tendons.
JOURNAL OF BIOMECHANICS
(2022)
Review
Chemistry, Multidisciplinary
Bingjie Zhang, Shanshan Li, Wenqing Shui
Summary: G protein-coupled receptors (GPCRs) are a large family of proteins that play significant roles in cellular and physiological responses. Post-translational modifications (PTMs) of GPCRs, such as glycosylation, phosphorylation, ubiquitination and palmitoylation, have important regulatory functions in receptor folding, biosynthesis, trafficking, dimerization and signaling. This review provides an overview of PTMs in GPCRs, focusing on their types, locations, cross talk and dynamic regulation, which can shed light on the molecular basis of GPCRs and contribute to structure-based drug discovery.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Svetlana Baskal, Dimitrios Tsikas
Summary: The reaction of L-lysine(Lys) with Glyoxal(GO) and methylglyoxal (MGO) was studied, and it was found that N-epsilon-Carboxymethyllysine (CML) and N-epsilon-carboxyethyllysine (CEL) were formed as advanced glycation end products (AGEs). The concentration of CML increased with higher temperature and MGO inhibited the formation of CML. The reaction of Lys with GO and MGO resulted in the formation of unidentified reaction products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Neil G. Rumachik, Stacy A. Malaker, Nicole K. Paulk
Summary: Advances in gene therapy product and process development have improved our understanding of the basic biology of recombinant AAV, with a particular focus on post-translational modifications of the capsid protein. Concerns about immunogenic responses to high doses of systemically administered rAAV have emphasized the importance of cataloging and tracking potentially immunogenic components of vector lots.
FRONTIERS IN IMMUNOLOGY
(2021)