Article
Health Care Sciences & Services
Eun-Ju Lee, Joohon Sung, Hyung-Lae Kim, Han-Na Kim
Summary: Based on a study of blood microbiota in healthy individuals, this research found that Proteobacteria accounts for more than 95% of the blood microbiota. Different age groups showed significant differences in the types and abundance of bacteria present in the blood, with the elderly group having higher levels of certain bacteria and lower levels of others. These findings provide meaningful evidence for the association between chronic diseases related to aging and metabolic endotoxemia and chronic inflammation.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Neil Marr, Richard Meeson, Elizabeth F. Kelly, Yongxiang Fang, Mandy J. Peffers, Andrew A. Pitsillides, Jayesh Dudhia, Chavaunne T. Thorpe
Summary: The study found that CD146+ cells migrate from the IFM to the injury site and participate in the healing process after tendon injury. Additionally, laminin-alpha 4 facilitates the recruitment of CD146+ cells at the injury site. Furthermore, a panel of circulating microRNAs dysregulated with tendon injury was identified.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Adam C. Swensen, Dusan Velickovic, Sarah M. Williams, Ronald J. Moore, Le Z. Day, Sherry Niessen, Sarah Hennessy, Camilo Posso, Mara Monetti, Wei-Jun Qian, Jon Jacobs, Laurence Whiteley, Ying Zhu, Paul D. Piehowski
Summary: Despite their small size, islets of Langerhans play a crucial role in maintaining systemic energy balance in the body. This study uses advanced technologies to investigate the protein profiles of different features within the islet, revealing unique protein signatures in the inner vasculature that indicate increased innervation and intra-islet neuron-like crosstalk. The data also prove useful in identifying localized structure-specific drug-target interactions.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Neurosciences
Andrea Pirone, Federica Ciregia, Giulia Lazzarini, Vincenzo Miragliotta, Maurizio Ronci, Mariachiara Zuccarini, Lorenzo Zallocco, Daniela Beghelli, Maria Rosa Mazzoni, Antonio Lucacchini, Giusti Laura
Summary: This study compares the protein profile of pig claustrum (CLA), putamen (PU), and insula (IN) using a comparative proteomic approach. The results show greater differences in protein spot expression between CLA and PU compared to CLA and IN. Deregulated proteins in CLA are associated with neurodegenerative and psychiatric disorders in humans. Analysis suggests the involvement of these anatomical structures in neurodegenerative diseases. Overall, this study provides insights into the proteomic analysis of pig CLA and its potential involvement in neurodegenerative and psychiatric disorders in humans.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Guanying Wang, Xiaojuan Ren, Xingping Zhang, Qingquan Wang, Tao Liu, Ning Deng, Deqi Yan
Summary: Insomnia characterized by wakefulness after sleep is associated with decreased sleep time and efficiency, as well as increased awakening time and numbers. Proteomic analysis identified 68 differentially expressed proteins enriched in humoral immune response, complement and coagulation cascades, and cholesterol metabolism. Hub proteins and potential molecular targets for clinical diagnosis and treatment were identified, suggesting a close relationship between the immune and metabolic systems in this type of insomnia.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Critical Care Medicine
Terry R. Schaid, Ian Lacroix, Mitchell J. Cohen, Kirk C. Hansen, Ernest E. Moore, Angela Sauaia, Alexis L. Cralley, Otto Thielen, William Hallas, Christopher Erickson, Sanchayita Mitra, Monika Dzieciatkowska, Christopher C. Silliman, Angelo D'Alessandro
Summary: This study compares the metabolomic and proteomic differences between normocalcemic and hypocalcemic trauma patients, and identifies distinct pathways and mechanisms associated with trauma-induced hypocalcemia, including mitochondrial dysfunction, inflammatory dysregulation, and aberrant coagulation pathways. These findings provide insights into the unique pathology of hypocalcemic trauma patients and pave the way for further investigation of mechanisms and interventions.
Article
Multidisciplinary Sciences
Yuanyuan Yang, Senyuan Hong, Cong Li, Jiaqiao Zhang, Henglong Hu, Xiaolong Chen, Kehua Jiang, Fa Sun, Qing Wang, Shaogang Wang
Summary: This study investigated the proteomics of urinary stones and crystals to identify the most frequently found proteins in kidney stones, revealing their involvement in inflammation and immune responses. Immunohistochemistry showed altered expression levels of certain proteins in renal tissues of kidney stone patients. These findings suggest potential diagnostic biomarkers and therapeutic targets for urolithiasis.
Article
Environmental Sciences
Yuan Liu, Yanlun Qiu, Qi Yin, Xinglong Li, Qunhua Bai, Yingli Li, Hong Xiao
Summary: The study investigated the mechanisms of Cr(VI) tolerance and reduction in the wild strain CM01 and its response to environmental pollution pressure at the molecular level. The iTRAQ technique identified differentially expressed proteins in domesticated CM01 related to Cr(VI) resistance. These findings were confirmed by RT-qPCR, showing consistency in gene expression levels. Additionally, domesticated CM01 displayed increased hydrophobicity, self-aggregation, glucose content, and SOD activity in response to Cr(VI) treatment.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Cell Biology
Takenori Omoto, Dilimulati Yimiti, Yohei Sanada, Minoru Toriyama, Chenyang Ding, Yuta Hayashi, Yasunari Ikuta, Tomoyuki Nakasa, Masakazu Ishikawa, Masayuki Sano, Minjung Lee, Takayuki Akimoto, Chisa Shukunami, Shigeru Miyaki, Nobuo Adachi
Summary: This study investigated the role of DICER in tendon maturation using Dicer conditional knockout (cKO) mice and identified key miRNAs in tendon maturation. The findings suggest that DICER plays an important role in tendon development, and miR-135a may be a crucial miRNA for tendon maturation and healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Evan T. Hoffman, Franziska E. Uhl, Loredana Asarian, Bin Deng, Chloe Becker, Juan J. Uriarte, Isaac Downs, Brad Young, Daniel J. Weiss
Summary: Chronic lung diseases, such as COPD and IPF, exhibit region-specific changes in extracellular matrix (ECM) composition, particularly in collagen and basement-membrane associated proteins. This study compares the ECM composition of whole decellularized lungs and specific anatomical lung regions in non-diseased, COPD, and IPF human lungs. The findings demonstrate distinct ECM signatures in different regions and reveal alterations in ECM composition specific to COPD and IPF, including enrichment of type-III collagen and fibulin in IPF aECM. This study provides methodology for future research and a dataset for identifying novel ECM targets for therapeutics.
Article
Biochemistry & Molecular Biology
Elizabeth Yohannes, Danielle L. Ippolito, Jennifer R. Damicis, Elisabeth M. Dornisch, Katherine M. Leonard, Peter G. Napolitano, Nicholas Ieronimakis
Summary: This study investigated the maternal plasma proteome in normal pregnancies using mass spectrometry. The results indicate an increase in proteins related to placental formation and fetal development. The concentration of ADAM12 protein is significantly correlated with gestational age and can be used to determine the gestational age in healthy pregnancies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Joshua D. Spitzberg, Scott Ferguson, Katherine S. Yang, Hannah M. Peterson, Jonathan C. T. Carlson, Ralph Weissleder
Summary: This study developed a technique called MASEV for multiplexed analysis of EVs, which allows the interrogation of thousands of individual EVs using multi-channel fluorescence staining. The findings reveal the presence of multiple biomarkers in single vesicles and the potential loss of rare EV subtypes through affinity purification. These findings demonstrate the importance of MASEV in uncovering the heterogeneity and biology of EVs and improving diagnostic specificity.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Aylin Acun, Ruben Oganesyan, Korkut Uygun, Heidi Yeh, Martin L. Yarmush, Basak E. Uygun
Summary: The extracellular matrix of the liver changes with age, affecting cell function, and donor age should be considered an important factor for bioengineering liver substitutes.
Article
Biotechnology & Applied Microbiology
Subhajit Konar, Scott M. Bolam, Brendan Coleman, Nicola Dalbeth, Sue R. McGlashan, Sophia Leung, Jillian Cornish, Dorit Naot, David S. Musson
Summary: Tendinopathy is characterized by pathological changes in tendon matrix composition, architecture, and stiffness, with inflammation also playing an important role. This study found that substrate stiffness affects tendon-derived cells and macrophages. Tendon-derived cells showed minor responses to substrate stiffness, while macrophages exhibited a more inflammatory phenotype on non-physiological stiffness substrates. These subtle variations in matrix stiffness may contribute to the onset and progression of tendinopathy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Melissa Baerenfaenger, Merel A. Post, Pieter Langerhorst, Karin Huijben, Fokje Zijlstra, Joannes F. M. Jacobs, Marcel M. Verbeek, Hans J. C. T. Wessels, Dirk J. Lefeber
Summary: The glycosylation of proteins plays an important role in neurological development and disease. Glycoproteomic studies on CSF provide valuable insights into brain glycosylation. However, it is important to consider that CSF proteins mainly originate from the blood, not reflecting brain glycosylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
D. Bardell, P. I. Milner, K. Goljanek-Whysall, M. J. Peffers
EQUINE VETERINARY JOURNAL
(2019)
Meeting Abstract
Orthopedics
Y. Ashraf Kharaz, M. Peffers, E. Comerford
OSTEOARTHRITIS AND CARTILAGE
(2019)
Article
Biochemical Research Methods
James R. Anderson, Marie M. Phelan, Luis M. Rubio-Martinez, Matthew M. Fitzgerald, Simon W. Jones, Peter D. Clegg, Mandy J. Peffers
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Biology
Howard Choi, Deborah Simpson, Ding Wang, Mark Prescott, Andrew A. Pitsillides, Jayesh Dudhia, Peter D. Clegg, Peipei Ping, Chavaunne T. Thorpe
Article
Biochemical Research Methods
Neil Marr, Mark Hopkinson, Andrew P. Hibbert, Andrew A. Pitsillides, Chavaunne T. Thorpe
BIOLOGICAL PROCEDURES ONLINE
(2020)
Review
Veterinary Sciences
Claire O'Brien, Neil Marr, Chavaunne Thorpe
Summary: This review discusses the biomechanical and biothermal effects of strain on the SDFT, as well as the impact of age-related alterations on injury risk. It emphasizes the importance of timely detection and adjustment of training protocols to prevent SDFT injuries.
EQUINE VETERINARY JOURNAL
(2021)
Article
Biology
Danae E. Zamboulis, Chavaunne T. Thorpe, Yalda Ashraf Kharaz, Helen L. Birch, Hazel R. C. Screen, Peter D. Clegg
Article
Cell Biology
Ioannis Kanakis, Moussira Alameddine, Leighton Folkes, Simon Moxon, Ioanna Myrtziou, Susan E. Ozanne, Mandy J. Peffers, Katarzyna Goljanek-Whysall, Aphrodite Vasilaki
Summary: Maternal diet can impact the development and health of offspring's skeletal muscles, with small non-coding RNAs playing a role in nutrition-associated programming. Maternal low protein diet was found to affect offspring skeletal muscle development, leading to decreased muscle fiber size in weanling offspring groups. The differential expression of multiple miRs, snoRNAs, and snRNAs suggests their involvement in key muscle-specific biological processes.
Article
Engineering, Biomedical
Dharmesh Patel, Danae E. Zamboulis, Ewa M. Spiesz, Helen L. Birch, Peter D. Clegg, Chavaunne T. Thorpe, Hazel R. C. Screen
Summary: The study compared the IFM structure-function specialization in human positional anterior tibial tendon and energy storing Achilles tendons, showing greater elasticity and fatigue resistance in the Achilles tendon. Changes in the proteome of the Achilles IFM with aging likely contribute to decreased fatigue resistance. Understanding these specializations and changes with aging is crucial for developing treatments and preventative measures for tendinopathy.
ACTA BIOMATERIALIA
(2021)
Article
Biology
Sidrah Jahangir, Peter John, Attya Bhatti, Muhammad Muaaz Aslam, Javaid Mehmood Malik, James R. Anderson, Mandy J. Peffers
Summary: This study aimed to identify potential serum biomarkers for rheumatoid arthritis in the Pakistani population. A total of 10 differentially expressed proteins were identified and pathway analysis predicted macrophage-related signaling pathways.
Article
Biochemistry & Molecular Biology
Panagiotis Balaskas, Katarzyna Goljanek-Whysall, Peter D. Clegg, Yongxiang Fang, Andy Cremers, Aibek Smagul, Tim J. M. Welting, Mandy J. Peffers
Summary: Osteoarthritis, the most common degenerative joint disorder, was studied using microarray analysis to identify differentially expressed microRNAs in cartilage. The role of miR-107 and miR-143-3p in chondrocyte mechanisms regulating proliferation, hypertrophy, and protein translation was confirmed through experiments in human primary chondrocytes.
Meeting Abstract
Pathology
P. Balaskas, K. Whysall, P. Clegg, T. Welting, M. Peffers
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2019)
Meeting Abstract
Pathology
Y. A. Kharaz, E. Comerford, M. Peffers
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2019)
Meeting Abstract
Pathology
M. J. Peffers, A. Smaghul, P. Balaska, Y. Fang, S. T. M. Welting
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2019)
Meeting Abstract
Pathology
A. Smagul, D. Simpson, S. Tew, M. J. Peffers
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2019)