Article
Biochemistry & Molecular Biology
Soran Mohammed, Natalie Ferry
Summary: The study compared the binding activity of mistletoe lectin isoform I (MLI) and Sambucus nigra agglutinin (SNA) towards sialic acid and beta-galactose through computational carbohydrate-protein docking and analysis of amino acid sequences, ultimately identifying their sialic acid binding domains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anabel Gonzalez-Gil, T. August Li, Jean Kim, Ronald L. Schnaar
Summary: Most human Siglecs are expressed on immune cells and can down-regulate the immune response when binding to complementary sialoglycans. They have potential as drug targets for modifying inflammation and as immune checkpoints in cancer. Endogenous human sialoglycan immune regulators are being identified and may provide opportunities for therapeutic targeting of Siglecs.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Anabel Gonzalez-Gil, T. August Li, Jean Kim, Ronald L. Schnaar
Summary: Most human Siglecs are expressed on immune cells, carry inhibitory domains, and bind to endogenous sialoglycans. They have potential as drug targets for inflammation and cancer immunotherapy. The specific ligands for human Siglecs are starting to be identified, providing new therapeutic opportunities.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Article
Neurosciences
Jannis Wissfeld, Ichiro Nozaki, Mona Mathews, Tamara Raschka, Christian Ebeling, Veit Hornung, Oliver Brustle, Harald Neumann
Summary: Genome-wide association studies have shown that mutations in the CD33 gene are linked to late-onset Alzheimer's disease. Deletion or Exon 2 deletion expression of CD33 in macrophages and microglia led to increased inflammatory pathways and activation of cytokines. The knockout of CD33 also resulted in enhanced phagocytosis of amyloid beta and bacterial particles, but potentially detrimental oxidative burst and inflammation.
Review
Biotechnology & Applied Microbiology
Zuzana Hlasova, Lucia Pazitna, Miroslav Ondrejovic, Jaroslav Katrlik
Summary: Small molecule inhibitors of neuraminidases are promising for treating influenza viruses. In vitro determination of their inhibition activity is important, but traditional methods may have interference issues. A lectin-based assay showed good sensitivity and avoided toxic reagents, offering benefits for NA inhibitor testing.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Immunology
Juliane Guenther, Sebastian Peter Galuska
Summary: Galectins are a diverse family of carbohydrate-binding proteins found in vertebrates, and their evolutionary history has been revealed through genomic analyses. The study highlights the origin and evolution of galectin family members in different animal groups, including primitive Bilateria and jawed vertebrates. Additionally, the research identifies a previously neglected galectin-related protein in Gnathostomata.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marco D'Addio, Jasmin Frey, Carlotta Tacconi, Catharina D. Commerford, Cornelia Halin, Michael Detmar, Richard D. Cummings, Vivianne Otto
Summary: The interactions between sialoglycans on lymphatic endothelial cells (LEC) and macrophage-expressed Siglec-1 play a critical role in regulating macrophage localization and phenotype. The densely clustered, sialylated glycans on the SCS floor LEC were identified as a key component in the macrophage niche, providing anchorage for Siglec-1(+) LN-SCS macrophages. Defective binding of sialoglycans by Siglec-1 results in reduced numbers of Siglec-1(+) macrophages with a less proliferative and more anti-inflammatory phenotype.
Article
Fisheries
Xiaohan Liu, Jiqing Gao, Xiao Wei, Xinze Zhang, Liping You, Yuanjin Liu, Lizhu Chen, Caili Liu, Chunxiao Sun, Xiuhui Tian, Quanli Zhou, Xiaomin Zhang
Summary: In this study, the potential mechanisms of PAMP binding and opsonic activities of a c-type lectin and a sialic acid-binding lectin from manila clam were investigated. The results showed that both lectins could bind to various pathogen-associated molecular patterns (PAMPs) and enhance the phagocytosis and encapsulation ability of hemocytes. These lectins may function as pattern recognition receptors (PRRs) involved in the innate immune responses of the clam.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Simon J. L. Petitjean, Wenzhang Chen, Melanie Koehler, Ravikumar Jimmidi, Jinsung Yang, Danahe Mohammed, Blinera Juniku, Megan L. Stanifer, Steeve Boulant, Stephane P. Vincent, David Alsteens
Summary: This study reveals that SARS-CoV-2 specifically binds to 9-O-acetylated sialic acid on cell surfaces and proposes novel inhibitors for viral infection. Understanding the molecular details of virus-cell binding and developing anti-viral strategies are urgent tasks in response to the emergence of highly transmissible SARS-CoV-2 variants.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Hirotaka Fudaba, Yasutomo Momii, Taisei Hirakawa, Kouhei Onishi, Daigo Asou, Wataru Matsushita, Yukari Kawasaki, Kenji Sugita, Minoru Fujiki
Summary: In this study, the expression of Siglec-15 on peritumoral macrophages was found to be significantly associated with longer overall survival in PCNSL patients, indicating a favorable outcome. Furthermore, Siglec-15 positivity in peritumoral macrophages and high-dose methotrexate-based chemotherapy were identified as independent predictors of overall survival.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Elizabeth R. Webster, Katherine N. Liu, Robert J. Rawle, Steven G. Boxer
Summary: This study investigates the impact of receptor length and rigidity on the mechanism of influenza virus entry. The results suggest that shorter and more flexible receptors increase the binding rate of the virus, while longer and more rigid receptors decrease the efficiency of lipid and content mixing.
Article
Physics, Fluids & Plasmas
Pedro A. Soria Ruiz, Falko Ziebert, Igor M. Kulic
Summary: This study investigates the physics of viral self-rolling states and reveals the mechanism of their motion, validating previous theories. The results indicate that viral rolling in the presence of enzymatic activity is statistically inevitable.
Article
Biochemistry & Molecular Biology
Robert Kerep, Tino Seba, Valentina Borko, Tin Weitner, Toma Keser, Gordan Lauc, Mario Gabricevic
Summary: Human serum alpha-1 acid glycoprotein is involved in the binding and transport of many drugs. Sialic acid groups play a major role in drug binding. The study found that different sialylation can result in different binding affinities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Mahdi Naseri, Maisha Maliha, Mostafa Dehghani, George P. Simon, Warren Batchelor
Summary: The development of a biosensor using MBL protein and PSM microparticles for detecting bacterial cells in water samples has been achieved. The biosensor shows a binary response, remaining blue in the presence of bacterial cells and changing color from blue to colorless in their absence.
Article
Biochemistry & Molecular Biology
Anderson Aparecido do Espirito Santo, Gustavo Troiano Feliciano
Summary: This study investigated the biomolecular recognition between ArtinM and HRP using molecular dynamics, showing that the binding free energy between them is extremely high without glycosylation, mainly due to van der Waals energy terms. With glycosylation, the binding energy significantly decreases, mainly due to electrostatic energy terms, increasing the selectivity of molecular recognition.
JOURNAL OF MOLECULAR MODELING
(2021)