Article
Oncology
Versha Tripathi, Pooja Jaiswal, Anshul Assaiya, Janesh Kumar, Hamendra Singh Parmar
Summary: This study found that AICAR has beneficial effects on TNBC cells, possibly by regulating mitochondrial biogenesis to switch off the Warburg effect and switch on the anti-Warburg effect.
CURRENT CANCER DRUG TARGETS
(2022)
Article
Chemistry, Multidisciplinary
Jun Ho Lee, Jung Yoon Jang, Young Hoon Kwon, Seung Ho Lee, Cheol Park, Yung Hyun Choi, Nam Deuk Kim
Summary: The effectiveness of rosemary extract (RE) in inducing myotube differentiation and inhibiting AICAR-induced muscle atrophy was evaluated in this study. The results showed that RE increased myotube thickness and promoted the expression of muscle differentiation-related proteins MyoD and myogenin. Furthermore, RE reduced the expression of muscle atrophy-related proteins FoxO3a, MAFbx/atrogin-1, and MuRF-1, while significantly increasing the expression of MyoD and myogenin.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Huimin Zhu, Wanbo Zeng, Tangna Zhao, Weiguo Shi, Xiao Dong, Aiping Zhang, Xiang Li, Liang Xu
Summary: This study synthesized six derivatives of AICAR and evaluated their anti-fatigue effects. The optimal compound ZHM-01 exhibited significant anti-fatigue effects in animal models, while also demonstrating good biological safety.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Organic
Noriko S. Tarashima, Yusuke Kumanomido, Katsuyuki Nakashima, Yoshiyuki Tanaka, Noriaki Minakawa
Summary: CDNs, second messengers composed of two purine nucleotides, have attracted intense research attention in recent years. This study reports the chemical synthesis of cyclic di-5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl monophosphate (c-di-ZMP) (1), which is constructed using phosphoramidite chemistry and ring-opening reaction of Hxa(DNP)-base.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Clinical Neurology
Praisy Joy, Vrisha Madhuri, Thomas Palocaren, Sweta Das, Suneetha Susan Cleave Abraham, Sophy Korula, Beena Koshy, John Jose, Mahalakshmi Chandran, Sumita Danda
Summary: This study reports the first case of ATIC deficiency, a rare genetic disorder of purine biosynthesis, from India. Early diagnosis can lead to early interventional therapy and genetic counselling.
BRAIN & DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Yuhei Nogi, Noriko Saito-Tarashima, Sangita Karanjit, Noriaki Minakawa
Summary: 5-aminoimidazole-4-carboxamide (Z-base) can pair with any canonical base and behaves physiologically as an A-like nucleobase, preferentially forming pairs with T. Z-base-containing DNA oligomers were synthesized and tested in enzyme-catalyzed reactions, showing that Klenow fragment DNA polymerase and Taq DNA polymerase incorporated dTTP as a complementary nucleotide to Z-base. These findings contribute to the development of new mutagenic antiviral nucleoside analogs.
Article
Chemistry, Multidisciplinary
Manki Son, Punit Mehra, Freddy T. Nguyen, Xiaojia Jin, Volodymyr B. Koman, Xun Gong, Michael A. Lee, Naveed A. Bakh, Michael S. Strano
Summary: There is an urgent need for real-time monitoring of chemotherapeutic activity within the human body to optimize drug dosimetry parameters and maximize efficacy while minimizing cytotoxicity. In this study, we developed near-infrared fluorescent nanosensors based on single walled carbon nanotubes that can monitor drug activity in real-time, even under in vivo conditions. The sensors can be made biocompatible by encapsulating them in poly(ethylene glycol) diacrylate hydrogels, and they have been shown to selectively detect the chemotherapeutic Temozolomide in human glioblastoma cells and mice. These sensors provide valuable information on therapeutic effectiveness by detecting and analyzing concentrations, flux, directional transport, and metabolic activity of chemotherapeutic agents.
Article
Food Science & Technology
Kyungae Jo, Woo Young Jang, Beom Sik Yun, Jin Soo Kim, Hyun-Sun Lee, Yeok Boo Chang, Hyung Joo Suh
Summary: The study found that deer antler extract can promote muscle differentiation, mitigate muscle atrophy, and increase the expression levels of related genes.
FOOD SCIENCE OF ANIMAL RESOURCES
(2021)
Article
Multidisciplinary Sciences
Masato Sasaki, Kazuo Yamamoto, Takeshi Ueda, Hayato Irokawa, Kouki Takeda, Ryoya Sekine, Fumie Itoh, Yutaka Tanaka, Shusuke Kuge, Nobuyuki Shibata
Summary: Tumor cells activate de novo purine synthesis to acquire large amounts of nucleotides. The expression of ALDH1L1, a key enzyme in this process, is often reduced in hepatocellular carcinoma (HCC). Our study showed that ALDH1L1 expression led to altered levels of serine and glycine, as well as accumulation of 5-aminoimidazole-4-carboxamide ribonucleotide (ZMP) due to the consumption of 10-formyltetrahydorofolate (10-fTHF) by ALDH1L1. Importantly, ALDH1L1-expressing cells exhibited reduced ZMP sensitivity and higher mitochondrial activity. Furthermore, genes related to oxidative phosphorylation were enriched in HCC patients with high ALDH1L1 expression.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Physical
Daniel Baranowski, Jerzy Boryski, Tomasz Ostrowski
Summary: The study showed that 7-(beta-D-ribofuranosyl)guanine promoted the preferential formation of 3-substituted product compared to guanosine when subjected to reaction conditions, with the minor formation of 1-methylated product. This finding opened up a new route for the synthesis of 3-methylguanine from guanine.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemistry & Molecular Biology
Hao-Lun Luo, Yi-Chen Lee, Yin-Lun Chang, Wei-Chi Hsu, Yen-Ting Wu, Jhen-Hao Jhan, Hui-Hui Lin, Yi-Ru Wu, Hung-Lung Ke, Hui-Ying Liu
Summary: UTUC has a high incidence rate in Taiwan and understanding the mechanisms underlying its growth and metastasis is crucial. In this study, miR-145-5p was found to downregulate ATIC protein expression and high ATIC expression was associated with poor prognosis in UTUC patients. Knockdown of ATIC inhibited the proliferation, migration, and invasion of UTUC cells, while miR-145-5p affected these cell functions by targeting the 3' untranslated regions of ATIC. RNA sequencing and Ingenuity Pathway Analysis identified downstream genes regulated by ATIC and regulated by miR-145-5p via ATIC. ATIC may serve as a valuable predictor of prognosis and a potential therapeutic target for UTUC.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Patrycja Koszalka, Barbara Kutryb-Zajac, Paulina Mierzejewska, Marta Tomczyk, Joanna Wietrzyk, Pawel K. Serafin, Ryszard T. Smolenski, Ewa M. Slominska
Summary: This study found that the metabolic product 4PYR, which accumulates in the blood of cancer patients, promotes lung metastasis through multiple mechanisms. In vivo studies showed that 4PYR did not affect primary tumor growth but facilitated lung metastasis. In vitro studies demonstrated that 4PYR affected extracellular adenine nucleotide metabolism and intracellular energy status, leading to increased permeability of lung ECs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Fatima Cerqueira, Marta Maia, Carla Gabriel, Rui Medeiros, Sara Cravo, Ana Isabel Ribeiro, Daniela Dantas, Alice Maria Dias, Lucilia Saraiva, Liliana Raimundo, Eugenia Pinto
Summary: Systemic mycoses are a major cause of morbidity and mortality in immunocompromised individuals. This study aimed to elucidate the mechanism of action of certain compounds with strong antifungal activity against Candida krusei and C. albicans strains, revealing a potential involvement of reactive oxygen species in the antifungal activity. Additionally, the association of certain compounds with fluconazole showed significant inhibition of a virulence mechanism in C. albicans.
Article
Chemistry, Applied
Ji Qi, Jingjun Yin, Donghong Li, Song Chen, Zhenguo Liu
Summary: An innovative and efficient synthesis of 5-amino-1H-imidazole-4-carboxamide (AIC) from commercially available hypoxanthine is described, with a highly efficient one-step manufacturing process achieved through the development of a key hydrolysis step and practical isolation, resulting in minimal environmental impact and significant reduction of production cost.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2021)
Article
Materials Science, Paper & Wood
Lian Li, Zhouyang Xiang
Summary: The study investigated the crystallization properties of acetylated xylans, revealing a significant impact of acetylation on the crystalline characteristics of xylans. Ethanol-precipitated acetylated xylans exhibited xylan diacetate crystal peaks at DS=2, while water-precipitated acetylated xylans showed the appearance of xylan diacetate crystal peaks at DS=1.96, indicating an abrupt formation of xylan diacetate crystal with changes in DS.
Article
Biochemistry & Molecular Biology
Delphine C. Douillet, Benoit Pinson, Johanna Ceschin, Hans C. Huerlimann, Christelle Saint-Marc, Damien Laporte, Stephane Claverol, Manfred Konrad, Marc Bonneu, Bertrand Daignan-Fornier
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Review
Cell Biology
Bertrand Daignan-Fornier, Benoit Pinson
Article
Genetics & Heredity
Roxane Marsac, Benoit Pinson, Christelle Saint-Marc, Maria Olmedo, Marta Artal-Sanz, Bertrand Daignan-Fornier, Jose-Eduardo Gomes
Article
Biology
Benoit Pinson, Johanna Ceschin, Christelle Saint-Marc, Bertrand Daignan-Fornier
Article
Genetics & Heredity
Christelle Saint-Marc, Johanna Ceschin, Claire Almyre, Benoit Pinson, Bertrand Daignan-Fornier
Article
Multidisciplinary Sciences
Diego Velazquez, Marcel Albacar, Chunyi Zhang, Carlos Calafi, Maria Lopez-Malo, Javier Torres-Torronteras, Ramon Marti, Sergey I. Kovalchuk, Benoit Pinson, Ole N. Jensen, Bertrand Daignan-Fornier, Antonio Casamayor, Joaquin Arino
SCIENTIFIC REPORTS
(2020)
Article
Genetics & Heredity
Jean-Marc Blouin, Cecile Ged, Ganeko Bernardo-Seisdedos, Txomin Cabantous, Benoit Pinson, Antoine Poli, Herve Puy, Oscar Millet, Laurent Gouya, Fanny Morice-Picard, Emmanuel Richard
Summary: Congenital erythropoietic porphyria is a rare genetic disorder characterized by UROS deficiency, resulting in ineffective erythropoiesis and skin fragility. Therapeutic options are limited, but inducing iron depletion through phlebotomies may effectively reduce porphyrin accumulation and improve disease symptoms without causing anemia.
MOLECULAR GENETICS AND METABOLISM REPORTS
(2021)
Article
Urology & Nephrology
Hannah Kaminski, Gabriel Marseres, Nathalie Yared, Marie-Julie Nokin, Vincent Pitard, Atika Zouine, Isabelle Garrigue, Severine Loizon, Myriam Capone, Xavier Gauthereau, Maria Mamani-Matsuda, Roxane Coueron, Raul V. Duran, Benoit Pinson, Isabelle Pellegrin, Rodolphe Thiebaut, Lionel Couzi, Pierre Merville, Julie Dechanet-Merville
Summary: mTOR inhibitors have been shown to enhance T cell function and reduce the incidence of CMV infection in kidney transplant recipients. This effect may be achieved by reducing the number of dysfunctional T cells.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Multidisciplinary Sciences
Corinne Clave, Witold Dyrka, Elizabeth A. Turcotte, Alexandra Granger-Farbos, Lea Ibaelosa, Benoit Pinson, Russell E. Vance, Sven J. Saupe, Asen Daskalov
Summary: Gasdermins are a family of proteins that control cell death reactions in both mammals and fungi. This study reveals the proteolytic regulation of Gasdermins in fungi, showing an evolutionary parallel between fungal and mammalian cell death pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Angele Tingaud-Sequeira, Elina Mercier, Vincent Michaud, Benoit Pinson, Ivet Gazova, Etienne Gontier, Fanny Decoeur, Lisa McKie, Ian J. Jackson, Benoit Arveiler, Sophie Javerzat
Summary: We have identified DCT encoding dopachrome tautomerase (DCT) as the eighth gene for oculocutaneous albinism. Loss of DCT function leads to eye hypopigmentation and retinal dystrophy. By investigating Dct mice, we found severe hypopigmentation in their retinal pigmented epithelium (RPE) from early stages, with specific cellular defects. Understanding the molecular regulation of retinal development and aging of the hypopigmented eye may help guide therapeutic strategies for patients with albinism.
Article
Food Science & Technology
Samuel Amintas, Pauline Beaumont, Charles Dupin, Isabelle Moranvillier, Isabelle Lamrissi, Elie Patel, Benjamin Fernandez, Alice Bibeyran, Julian Boutin, Tristan Richard, Stephanie Krisa, Francois Moreau-Gaudry, Aurelie Bedal, David Cappellen, Benoit Pinson, Veronique Vendrely, Sandrine Dabernat
Summary: This study investigated the differential impact of resveratrol on tumor cells and healthy cells by evaluating its metabolism and the expression of UGT1A genes. The findings revealed that resveratrol was more cytotoxic to tumor cells with downregulated UGTs, while overexpression of UGT1A10 gene reduced its cytotoxicity. Resveratrol also affected the cell cycle, nucleotide metabolism, and global transcription and translation in intestinal sensitive tumor cells. This study enhances our understanding of the antitumor action of resveratrol and provides insights for its selective treatment in colorectal tumors.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Immunology
Florent Guerville, Marine Vialemaringe, Celine Cognet, Pierre Duffau, Estibaliz Lazaro, Charles Cazanave, Fabrice Bonnet, Olivier Leleux, Rodrigue Rossignol, Benoit Pinson, Camille Tumiotto, Frederic Gabriel, Victor Appay, Julie Dechanet-Merville, Linda Wittkop, Benjamin Faustin, Isabelle Pellegrin
Summary: The objective of this study was to determine the contribution of inflammasome activation in chronic low-grade systemic inflammation observed in patients with HIV on long-term suppressive antiretroviral therapy. Inflammasome activation was measured by cytokine analysis in patient serum and ex vivo stimulation of primary monocytes. The results showed higher levels of IL-18 in HIV-positive patients compared to HIV-negative controls, along with increased secretion of IL-1 beta in response to inflammasome stimulation. Oxidative stress was also observed in patient sera. In conclusion, HIV infection with long-term effective ART is associated with inflammasome activation and increased monocyte activation, possibly mediated by oxidative stress.
Article
Cell Biology
Maelle Duperray, Fanny Hardet, Elodie Henriet, Christelle Saint-Marc, Eric Boue-Grabot, Bertrand Daignan-Fornier, Karine Masse, Benoit Pinson
Summary: This study investigates the developmental requirements for purine metabolism using the Xenopus laevis model. The research shows that purine pathway genes are mainly expressed in nervous and muscular embryonic tissues and are essential for myogenesis. Knockdown of certain genes in the pathway results in defects in muscle formation.
