Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 288, Issue 25, Pages 18243-18248Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.468850
Keywords
-
Categories
Funding
- National Institutes of Health [AI070603, AI077283]
- Flow Cytometry Shared Resource, Hollings Cancer Center, Medical University of South Carolina [P30 CA138313]
- SmartState Endowed Chair Program of South Carolina
Ask authors/readers for more resources
Integrins play important roles in regulating a diverse array of cellular functions crucial to the initiation, progression, and metastasis of tumors. Previous studies have shown that a majority of integrins are folded by the endoplasmic reticulum chaperone gp96. Here, we demonstrate that the dimerization of integrin alpha L and beta 2 is highly dependent on gp96. The alpha I domain (AID), a ligand binding domain shared by seven integrin alpha-subunits, is a critical region for integrin binding to gp96. Deletion of AID significantly reduced the interaction between integrin alpha L and gp96. Overexpression of AID intracellularly decreased surface expression of gp96 clients (integrins and Toll-like receptors) and cancer cell invasion. The alpha 7 helix region is crucial for AID binding to gp96. A cell-permeable alpha 7 helix peptide competitively inhibited the interaction between gp96 and integrins and blocked cell invasion. Thus, targeting the binding site of alpha 7 helix of AID on gp96 is potentially a new strategy for treatment of cancer metastasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available