Article
Biochemistry & Molecular Biology
Hao Cui, Xiaowen Cheng, Tahira Batool, Xiao Zhang, Jin-Ping Li
Summary: The study suggests that a defect in the key enzyme glucuronyl C5-epimerase (Hsepi) involved in heparan sulfate biosynthesis may lead to lung developmental failure in mice. Despite no significant morphological differences before E16.5, mutant animals show abnormal lung structure at E17.5-18.5, with impaired AT1 cell differentiation possibly associated with reduced lung vascularization.
Article
Biochemistry & Molecular Biology
Hao Cui, Zhaoguang Wang, Tianji Zhang, Jin-ping Li, Jianping Fang
Summary: The translation explores the role of Heparan sulfate (HS) in protein recognition and interaction, highlighting the importance of specific sulfation and epimerization patterns modulated by Golgi-localized enzymes. The study investigates the effects of restoring Hsepi in mutant MEF cells, showing increased IdoA residues and rescued cell signaling, although Hsepi knockout did not influence cellular transport or enzymatic activity of 2OST. These findings suggest potential differences in regulatory mechanisms for 2OST and Hsepi.
Review
Biochemistry & Molecular Biology
Anthony J. J. Hayes, James Melrose
Summary: Heparan sulfate is a complex glycosaminoglycan that plays important roles in various biological processes through interactions with extracellular ligands and cellular receptors. Its chain lengths and sequences can vary, providing structural diversity. Heparan sulfate has regulatory properties in tissue development, wound healing, immune regulation, inflammation, and tumor development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Mahmudul Hasan, Hamed Khakzad, Lotta Happonen, Anders Sundin, Johan Unge, Uwe Mueller, Johan Malmstrom, Gunilla Westergren-Thorsson, Lars Malmstrom, Ulf Ellervik, Anders Malmstrom, Emil Tykesson
Summary: DS-epi1 catalyzes a key step in the biosynthesis of the glycosaminoglycan dermatan sulfate, and a novel mechanism involving a His/double-Tyr motif has been proposed based on structural and docking studies. The high structural similarity of DS-epi1 to bacterial polysaccharide lyases suggests its importance in various diseases, and its structure provides a starting point for designing active site inhibitors.
Review
Biochemistry & Molecular Biology
J. Michael Sorrell, Arnold Caplan
Summary: White adipose tissues are crucial endocrine organs that release adipokines, affecting other major organ systems. The development and functions of adipose tissues rely heavily on the glycosaminoglycan heparan sulfate, which regulates cellular physiology and communication. Understanding the role of heparan sulfates in the regulation of adipokine production and release could provide valuable insights into tissue engineering and experimental studies.
Article
Chemistry, Multidisciplinary
Dylan Mah, Yanan Zhu, Guowei Su, Jing Zhao, Ashely Canning, James Gibson, Xuehong Song, Eduardo Stancanelli, Yongmei Xu, Fuming Zhang, Robert J. Linhardt, Jian Liu, Lianchun Wang, Chunyu Wang
Summary: Apolipoprotein E (ApoE)'s epsilon 4 allele is the most important genetic risk factor for late onset Alzheimer's Disease (AD). Heparan sulfate (HS) on the cell surface acts as a cofactor for the interaction between ApoE and LRP1, as well as the prion-like spread of tau pathology between cells. 3-O-sulfated HS has been linked to AD through its interaction with tau, and increased levels of 3-O-sulfated HS and 3-O-sulfotransferases have been observed in the AD brain. This study characterizes the interactions between ApoE and HS in different ApoE isoforms, and suggests that the interplay between 3-O-sulfated HS, tau, and ApoE isoforms may modulate AD risk.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Applied
Tianji Zhang, Mingjia Yu, Honglian Li, Marco Maccarana, Wei Zhang, Deling Shi, Ying Kan, Xiao Zhang, Lianli Chi, Ulf Lindahl, Hongmei Li, Jin-ping Li, Tianwei Tan
Summary: This study investigated the functional interactions between glucuronyl 5-epimerase (Hsepi) and hexuronyl 2-O-sulfotransferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st) using an isotope exchange approach. The results showed that the binding of these enzymes plays an important role in the efficiency of substrate conversion. Furthermore, experimental evidence suggested the formation of a functional complex between Hsepi and Hs6st in cells, and the inability to achieve simultaneous 2-O and 6-O sulfation in vitro.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemistry & Molecular Biology
Erpan Ahat, Yuefan Song, Ke Xia, Whitney Reid, Jie Li, Sarah Bui, Fuming Zhang, Robert J. Linhardt, Yanzhuang Wang
Summary: This study reveals that disrupting the Golgi structure affects the synthesis and secretion of glycosaminoglycans, leading to increased HS synthesis and decreased CS synthesis. The sulfation process of HS and CS is also altered in this case.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Samuel G. Holmes, Balaji Nagarajan, Umesh R. Desai
Summary: This study discovered novel compact topologies of heparan sulfate (HS) that are influenced by the 3-O-sulfation of cis-idoA residues. The transition in HS topology is driven by rotations that reduce like-charge repulsion, release water molecules, and establish specific interactions. These findings reveal a dynamic sulfation code in natural HS that could be utilized for selective recognition of target proteins.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Fatemah O. F. O. Alshammari, Yousef M. Al-Saraireh, Ahmed M. M. Youssef, Yahya M. Al-Sarayra, Hamzeh Mohammad Alrawashdeh
Summary: The study found intense glypican-1 expression in all breast cancer histopathological subtypes, with a significant difference in expression compared to normal breast tissue. Additionally, a higher expression of glypican-1 was observed in advanced grade and larger tumor size breast cancer patients, but showed no obvious relationship with other biomarkers.
ONCOTARGETS AND THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Julius Benicky, Miloslav Sanda, Aswini Panigrahi, Jian Liu, Zhangjie Wang, Vijayakanth Pagadala, Guowei Su, Radoslav Goldman
Summary: Sulf-2 is an extracellular enzyme involved in the editing of heparan sulfate. We introduce synthetic HS oligosaccharides and use a recombinant Sulf-2 protein to quantify its enzymatic activity. This assay offers a sensitive and specific measurement of the heparan 6-O-endosulfatase activity.
Article
Biochemistry & Molecular Biology
Dominique Manikowski, Georg Steffes, Jurij Froese, Sebastian Exner, Kristina Ehring, Fabian Gude, Daniele Di Iorio, Seraphine V. Wegner, Kay Grobe
Summary: Morphogens play a crucial role in cell differentiation in a concentration dependent manner. In the case of Hedgehog (Hh) morphogens, they spread from their source to guide growth and pattern formation in the Drosophila wing disc. Heparan sulfate (HS) expression is essential for this process, and it has been found that HS directly regulates Hh signaling and spread in the wing disc epithelium. Interfering with this regulation alters Hh signaling range and gradient robustness.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Wei Hao, Bo Ma, Ziheng Li, Xiaoyu Wang, Xiaopan Gao, Yaohao Li, Bo Qin, Shiying Shang, Sheng Cui, Zhongping Tan
Summary: The study found that the spike proteins and subunits of SARS-CoV-2, SARS-CoV, and MERS-CoV can bind to heparan sulfate (HS) in a sulfation-dependent manner, with no binding to sialic acid residues detected. This suggests that HS binding may be a general mechanism for these coronaviruses to attach to host cells.
Article
Oncology
Takamasa Kinoshita, Hiroyuki Tomita, Hideshi Okada, Ayumi Niwa, Fuminori Hyodo, Tomohiro Kanayama, Mikiko Matsuo, Yuko Imaizumi, Takahiro Kuroda, Yuichiro Hatano, Masafumi Miyai, Yusuke Egashira, Yukiko Enomoto, Noriyuki Nakayama, Shigeyuki Sugie, Kazu Matsumoto, Yu Yamaguchi, Masayuki Matsuo, Hideaki Hara, Toru Iwama, Akira Hara
Summary: Reduced expression of Heparan sulfate in the vascular endothelium of the brain can suppress glioblastoma growth and neovascularization in mice.
Article
Clinical Neurology
Po-Yu Lin, Jia-Horung Hung, Chao-Kai Hsu, Yao-Tsung Chang, Yuan-Ting Sun
Summary: Schwartz-Jampel syndrome is a rare autosomal recessive disease caused by mutation in the HSPG2 gene. A new pathogenic mutation site has been identified through whole-exome sequencing, leading to the syndrome. The mutation was shown to have pathogenic effects through in silico analysis, clinical presentations, and cellular function assessments.
FRONTIERS IN NEUROLOGY
(2021)
Article
Multidisciplinary Sciences
Yuya Ohhara, Yuko Shimada-Niwa, Ryusuke Niwa, Yasunari Kayashima, Yoshiki Hayashi, Kazutaka Akagi, Hitoshi Ueda, Kimiko Yamakawa-Kobayashi, Satoru Kobayashi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biochemistry & Molecular Biology
Tomomi Izumikawa, Katsufumi Dejima, Yukiko Watamoto, Kazuko H. Nomura, Nanako Kanaki, Marika Rikitake, Mai Tou, Daisuke Murata, Eri Yanagita, Ai Kano, Shohei Mitani, Kazuya Nomura, Hiroshi Kitagawa
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Cell Biology
Masahiko Takemura, Hiroshi Nakato
JOURNAL OF CELL SCIENCE
(2017)
Article
Developmental Biology
Katsufumi Dejima, Sukryool Kang, Shohei Mitani, Pamela C. Cosman, Andrew D. Chisholm
Article
Biochemistry & Molecular Biology
Sayaka Akiyoshi, Kazuko H. Nomura, Katsufumi Dejima, Daisuke Murata, Ayako Matsuda, Nanako Kanaki, Tetsuro Takaki, Hiroyuki Mihara, Takayuki Nagaishi, Shuhei Furukawa, Keiko-Gengyo Ando, Sawako Yoshina, Shohei Mitani, Akira Togayachi, Yoshinori Suzuki, Toshihide Shikanai, Hisashi Narimatsu, Kazuya Nomura
Article
Multidisciplinary Sciences
Rieko Imae, Katsufumi Dejima, Eriko Kage-Nakadai, Hiroyuki Arai, Shohei Mitani
SCIENTIFIC REPORTS
(2016)
Correction
Medicine, Research & Experimental
Woojin Kang, Yuichirou Harada, Kenji Yamatoya, Natsuko Kawano, Seiya Kanai, Yoshitaka Miyamoto, Akihiro Nakamura, Mami Miyado, Yoshiki Hayashi, Yoko Kuroki, Hidekazu Saito, Yasuhiro Iwao, Akihiro Umezawa, Kenji Miyado
LABORATORY INVESTIGATION
(2020)
Article
Medicine, Research & Experimental
Woojin Kang, Yuichirou Harada, Kenji Yamatoya, Natsuko Kawano, Seiya Kanai, Yoshitaka Miyamoto, Akihiro Nakamura, Mami Miyado, Yoshiki Hayashi, Yoko Kuroki, Hidekazu Saito, Yasuhiro Iwao, Akihiro Umezawa, Kenji Miyado
LABORATORY INVESTIGATION
(2020)
Review
Biology
Yoshiki Hayashi, Yuto Yoshinari, Satoru Kobayashi, Ryusuke Niwa
CURRENT OPINION IN INSECT SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Marika Rikitake, Ayako Matsuda, Daisuke Murata, Katsufumi Dejima, Kazuko H. Nomura, Karen L. Abbott, Shohei Mitani, Kazuya Nomura
JOURNAL OF BIOCHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Yuji Suehiro, Sawako Yoshina, Tomoko Motohashi, Satoru Iwata, Katsufumi Dejima, Shohei Mitani
Summary: The study successfully applied the TMP/UV mutagenesis method to C. elegans, creating a gene mutation library and discovering 981 novel mutations in the whole genome. By eliminating false positives through machine learning, the accuracy of mutation identification reached over 95%.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Yoshiki Hayashi, Arisa Shibata, Keisuke Kamimura, Satoru Kobayashi
Summary: The study investigated the functions of syndecan and perlecan in the Drosophila GSC niche and found that while both are important components for GSC maintenance, their effects on GSC numbers are distinct. Interestingly, overexpression of syndecan and perlecan did not cause an expansion of the GSC niche. These findings suggest that HSPG genes contribute to GSC maintenance through multiple mechanisms and pave the way for a deeper understanding of ECM functions in the stem cell niche.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2021)
Article
Multidisciplinary Sciences
Katsufumi Dejima, Rieko Imae, Yuji Suehiro, Keita Yoshida, Shohei Mitani
Summary: This study investigates the role of dsRNA in gene expression regulation and identifies ZIPT-9 as a negative regulator of systemic RNAi in C. elegans.
Article
Biochemistry & Molecular Biology
Nanako Kanaki, Ayako Matsuda, Katsufumi Dejima, Daisuke Murata, Kazuko H. Nomura, Takashi Ohkura, Keiko Gengyo-Ando, Sawako Yoshina, Shohei Mitani, Kazuya Nomura