Review
Oncology
Anne Liu, Yanyu Zhu, Weiping Chen, Glenn Merlino, Yanlin Yu
Summary: PTEN is a critical tumor suppressor gene with multifunctional phosphatase activity, and its protein-phosphatase activity plays a crucial role in tumor progression, although its mechanism is not fully understood.
Review
Neurosciences
Massimo M. Onesto, Caitlin A. Short, Sarah K. Rempel, Timothy S. Catlett, Timothy M. Gomez
Summary: Growth cones navigate through developing organisms by probing extracellular cues, with the ability of various growth factors to guide axons now underappreciated. In vitro experiments have shown the effects of these growth factors on axon morphogenesis and signaling mechanisms.
FRONTIERS IN NEUROSCIENCE
(2021)
Review
Cell Biology
Eloisa Herrera, Augusto Escalante
Summary: The development of the nervous system involves neurogenesis, neuronal specification, axonal navigation, and circuits assembly. The growth cone located at the tip of the axon plays a crucial role in guiding axons towards their final targets. The transcriptional control and epigenetic regulation of axon guidance molecules are important for determining specific axonal behaviors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Laura H. John, Fiona B. Naughton, Mark S. P. Sansom, Andreas Haahr Larsen
Summary: Phosphatase and tensin homologue (PTEN) and SH2-containing inositol 5 '-phosphatase 2 (SHIP2) are structurally and functionally similar, but their C2 domains have different roles in membrane interactions. The C2 domain of PTEN strongly interacts with anionic lipids and contributes to membrane recruitment, while the C2 domain of SHIP2 has weaker binding affinity for anionic membranes. The C2 domain in SHIP2 enhances the catalytic activity of the phosphatase domain through allosteric interdomain changes.
Article
Multidisciplinary Sciences
Florent Peglion, Lavinia Capuana, Isabelle Perfettini, Laurent Boucontet, Ben Braithwaite, Emma Colucci-Guyon, Emie Quissac, Karin Forsberg-Nilsson, Flora Llense, Sandrine Etienne-Manneville
Summary: Loss of PTEN promotes cell migration while targeting AMPK activity can counteract this effect. This suggests the important regulatory roles of PTEN and AMPK in tumor invasion and provides new therapeutic opportunities for glioblastoma.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Elisa Savino, Fabrizia Claudia Guarnieri, Jin-Wu Tsai, Anna Corradi, Fabio Benfenati, Flavia Valtorta
Summary: Mutations in the PRRT2 gene are the main cause of various paroxysmal neurological diseases, affecting neurotransmitter release regulation and actin cytoskeleton dynamics during synaptogenesis. The PRRT2 protein plays a crucial role in growth cone morphology during neuronal development, with abnormal PRRT2 leading to changes in growth cone shape and actin cytoskeleton.
Article
Biophysics
Ian Costa Paixao, Natsuki Mizutani, Makoto Matsuda, Rizki Tsari Andriani, Takafumi Kawai, Atsushi Nakagawa, Yoshifumi Okochi, Yasushi Okamura
Summary: Voltage-sensing phosphatase (VSP) consists of the voltage sensor domain (VSD) similar to that of voltage-gated ion channels and the cytoplasmic phosphatase region with remarkable similarity to PTEN. Depolarization of the membrane activates VSD, leading to dephosphorylation of PIPs. A bioinformatics search revealed amino acid diversity in VSP orthologs, particularly at a critical site in PTEN, and subsequent mutational analyses were performed to investigate the significance of this diversity.
BIOPHYSICAL JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Miguel E. Dominguez-Romero, Paula G. Slater
Summary: The growth cone is a critical structure during neuronal development and regeneration, detecting and integrating signals to reach its final destination. After neuronal injuries in mammals, only axons in the peripheral nervous system are able to regenerate, while the ones from the central nervous system fail to do so.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Yimin Zou
Summary: Growth cones interact with each other during axon pathfinding through regulated protein-protein interactions. This local signaling mechanism enhances the sensitivity of growth cones to shallow Wnt gradients, leading to precise and organized turning.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Leire Torices, Caroline Nunes-Xavier, Jose I. Lopez, Rafael Pulido
Summary: PTEN is a tumor suppressor gene frequently mutated in human tumors, and its mutations are the diagnostic marker for PTEN Hamartoma Tumor Syndrome (PHTS), a disorder characterized by multiple hamartomas, cancer predisposition, and neurodevelopmental alterations. This study generated and characterized anti-PTEN monoclonal antibodies recognizing the PTEN C2-domain, which can be used to study the expression and function of PTEN isoforms and mutations associated with disease. These antibodies are suitable for investigating the pathogenicity of PTEN C-terminal truncations that have lost the PTEN C-terminal epitopes but retain stability and function.
Article
Biology
Blake A. Creighton, Simone Afriyie, Deepa Ajit, Cristine R. Casingal, Kayleigh M. Voos, Joan Reger, April M. Burch, Eric Dyne, Julia Bay, Jeffrey K. Huang, Eva S. Anton, Meng-Meng Fu, Damaris N. Lorenzo
Summary: Variants in the high-confidence autism spectrum disorder (ASD) gene ANK2 target both the ubiquitously expressed 220 kDa ankyrin-B and the neurospecific 440 kDa ankyrin-B (AnkB440) isoforms, affecting axonal collateral branching and growth cone collapse. Deficits in AnkB440 response to repellent cues may contribute to the pathogenicity of ANK2 variants.
Article
Biochemistry & Molecular Biology
Silvia Vicenzi, Lisa Foa, Robert J. Gasperini
Summary: The neurotransmitter serotonin can act as both an attractive and repulsive guidance cue for rodent sensory neurons in vitro, with extracellular gradients of 50 mu M serotonin inducing attraction mediated by the serotonin 5-HT2a receptor and 100 mu M serotonin inducing repulsion mediated by the 5-HT1b receptor. High resolution imaging of growth cones showed that these receptors signal through their canonical pathways to shape neuronal circuit formation during development.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Andreia Teixeira-Castro, Joao Carlos Sousa, Carmen Vieira, Joana Pereira-Sousa, Daniela Vilasboas-Campos, Fernanda Marques, Perpetua Pinto-do-O, Patricia Maciel
Summary: This study explores the use of the worm Caenorhabditis elegans as a model to visualize the effects of molecular cues on axonal guidance and nervous system structure and behavior. Students observed the behavior of C. elegans and learned about the deleterious effects of gene malfunctioning in neuron response to biochemical cues. They also discovered the similarities between abnormal axon migration in C. elegans and humans.
Article
Plant Sciences
Collins Kipngetich Tanui, Divine Yutefar Shyntum, Precious K. Sedibane, Daniel Bellieny-Rabelo, Lucy N. Moleleki
Summary: The study found that the bacterium Pb 1692 exhibited chemotactic responses to various ligands and identified multiple MCP proteins. Four MCP mutants were not affected in virulence but showed significant attenuation in their ability to attach to potato leaves.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Rameen Forghani, Aravind Chandrasekaran, Garegin Papoian, Edward Giniger
Summary: This article proposes a fundamentally different, probabilistic model of axon growth based on the stochastic dynamics of actin networks. The model is supported by results from live imaging and computational simulations, and it offers explanations for longstanding puzzles in axon growth and guidance mechanisms. The probabilistic nature of actin dynamics also has implications for other processes of cell morphology and motility.
Article
Developmental Biology
Alexandra Krol, Steven J. Henle, Lisa V. Goodrich
Review
Biotechnology & Applied Microbiology
Bharath Wootla, Jens O. Watzlawik, Nikolaos Stavropoulos, Nathan J. Wittenberg, Harika Dasari, Murtada A. Abdelrahim, John R. Henley, Sang-Hyun Oh, Arthur E. Warrington, Moses Rodriguez
EXPERT OPINION ON BIOLOGICAL THERAPY
(2016)
Article
Biology
Lucas P. Carlstrom, Jacob H. Hines, Steven J. Henle, John R. Henley
Article
Biology
Jacob H. Hines, Steven J. Henle, Lucas P. Carlstrom, Mohammad Abu-Rub, John R. Henley
Article
Neurosciences
Steven J. Henle, Gordon Wang, Ellen Liang, May Wu, Mu-ming Poo, John R. Henley
JOURNAL OF NEUROSCIENCE
(2011)
Review
Neurosciences
Takuro Tojima, Jacob H. Hines, John R. Henley, Hiroyuki Kamiguchi
NATURE REVIEWS NEUROSCIENCE
(2011)
Article
Multidisciplinary Sciences
Zheyan Chen, Han Lee, Steven J. Henle, Thomas R. Cheever, Stephen C. Ekker, John R. Henley
Article
Oncology
Dominique M. Higgins, Ruisi Wang, Brian Milligan, Mark Schroeder, Brett Carlson, Jenny Pokorny, Samuel H. Cheshier, Fredric B. Meyer, Irving L. Weissman, Jann N. Sarkaria, John R. Henley
Article
Cell Biology
Evelyn C. Aviles, Alexandra Krol, Steven J. Henle, Jessica Burroughs-Garcia, Michael R. Deans, Lisa Goodrich
Summary: The polarized flow of information in neural circuits relies on the organization of neurons, their processes, and their synapses. Fat3, a protein similar to Fat cadherins, plays a crucial role in the development of polarized circuits in the mouse retina. It binds to cytoskeletal regulators and synaptic proteins, coordinating different cell behaviors and regulating synapse localization.
