Article
Biology
Sang Soo Lee, Jun Gyou Park, Eunhong Jang, Seung Hun Choi, Subin Kim, Ji Won Kim, Mi Sun Jin
Summary: The W546A mutation in Human ATP-binding cassette transporter subfamily B6 (ABCB6) alters the conformational dynamics, resulting in reduced substrate affinity and facilitating its release from the transporter.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Vincent Chaptal, Veronica Zampieri, Benjamin Wiseman, Cedric Orelle, Juliette Martin, Kim-Anh Nguyen, Alexia Gobet, Margot Di Cesare, Sandrine Magnard, Waqas Javed, Jad Eid, Arnaud Kilburg, Marine Peuchmaur, Julien Marcoux, Luca Monticelli, Martin Hogbom, Guy Schoehn, Jean-Michel Jault, Ahcene Boumendjel, Pierre Falson
Summary: This study resolved three outward-facing conformations of BmrA, a multidrug ABC transporter, using x-ray crystallography and cryo-electron microscopy. It was found that two R6G molecules bind to the drug-binding cavity, inducing a rearrangement of TM1-2 and highlighting local flexibility. Simulations showed that in the absence of R6G, the cavity quickly closed after drug release, while in the presence of R6G, the cavity remained open.
Article
Pharmacology & Pharmacy
Yubing Dong, Yi Lin, Wanyu Liu, Wei Zhang, Yinong Jiang, Wei Song
Summary: The study investigated the lipid profile in hypertensive disorders of pregnancy (HDP) patients, finding significantly altered levels of plasma triglycerides, total cholesterol, LDL-C, and HDL-C compared to normotensive controls. ANP was shown to inhibit ABCA1 and ABCG1 expression at both mRNA and protein levels, leading to impaired cholesterol efflux, possibly explaining the decreased HDL-C levels in HDP patients.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Latifa Alketbi, Abeer Al-Ali, Iman M. Talaat, Qutayba Hamid, Khuloud Bajbouj
Summary: Colorectal cancer (CRC) is a common malignancy worldwide, ranked fourth in cancer-related deaths. Mutations in oncogenes, tumor suppressor genes, and DNA repair genes contribute to the development of CRC. Chemoresistance is one of the major challenges in CRC treatment, and the overexpression of ATP-binding cassette (ABC) transporters has been identified as a facilitating mechanism for chemoresistance. ABC transporters are involved in lipid transport and homeostasis, which play a crucial role in tumor development and progression. Altered expression of ABCA transporters in CRC is associated with poor prognosis and overall survival. This minireview suggests that ABCA transporters could serve as potential diagnostic and therapeutic targets in CRC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Fiza Liaquat, Muhammad Farooq Hussain Munis, Samiah Arif, Muhammad Aamir Manzoor, Urooj Haroon, Iftikhar Hussain Shah, Muhammad Ashraf, Hyun Seok Kim, Shengquan Che, Liu Qunlu
Summary: This study investigated the differential gene expression of Schima superba under Mn metal stress through transcriptomic analysis. The differentially expressed genes were found to be involved in defense, signaling pathways, oxidative burst, transcription factors, and stress responses. The results provide insights into the metal resistance mechanisms and contribute to the understanding of heavy metal tolerance and detoxification.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yaolei Mi, Xue Cao, Xuewen Zhu, Weiqiang Chen, Xiangxiao Meng, Huihua Wan, Wei Sun, Sifan Wang, Shilin Chen
Summary: This study identified and characterized 113 ABC transporters in C. sativa, and proposed seven core transporters that may participate in cannabinoid transport. These candidate genes showed high correlation with cannabinoid biosynthetic pathway genes and content, and were highly expressed in cannabinoid biosynthesis and accumulation sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Jingxi Liang, Xiuna Yang, Tianyu Hu, Yan Gao, Qi Yang, Haitao Yang, Wei Peng, Xiaoting Zhou, Luke W. Guddat, Bing Zhang, Zihe Rao, Fengjiang Liu
Summary: This study used single particle cryo-electron microscopy (cryo-EM) to determine the structure of the trehalose-specific transporter in Mycobacterium tuberculosis and demonstrated the potential of this technique in exploring dynamic structures of transporters. It provides insights into the trehalose recycling mechanism in Mycobacterium tuberculosis.
Article
Pharmacology & Pharmacy
Louise Breuil, Nora Ziani, Sarah Leterrier, Gaelle Hugon, Fabien Caille, Viviane Bouilleret, Charles Truillet, Maud Goislard, Myriam El Biali, Martin Bauer, Oliver Langer, Sebastien Goutal, Nicolas Tournier
Summary: This study investigated the impact of CYP inducers and inhibitors on the brain and plasma kinetics of [C-11]metoclopramide using PET imaging. The results showed that CYP induction or inhibition had negligible effects on the plasma kinetics and metabolism of [C-11]metoclopramide, but ritonavir significantly increased brain penetration.
Article
Biochemistry & Molecular Biology
Silpa Narayanan, Zhuo-Xun Wu, Jing-Quan Wang, Hansu Ma, Nikita Acharekar, Jagadish Koya, Sabesan Yoganathan, Shuo Fang, Zhe-Sheng Chen, Yihang Pan
Summary: In this study, GS-9973 showed efficacy in overcoming ABCG2-mediated multidrug resistance in non-small cell lung cancer cells. The drug reversed drug resistance by blocking ABCG2 efflux activity and downregulating ABCG2 expression at the protein level, suggesting it as a potential strategy to overcome MDR in cancers.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Chemistry, Applied
Alba M. Garcia-Lino, Alex Gomez-Gomez, Dafne Garcia-Mateos, Alvaro de la Fuente, Ana I. Alvarez, Oscar J. Pozo, Gracia Merino
Summary: ABCG2 is involved in the secretion of several compounds in milk, with higher levels of tryptophan-related compounds in wild-type mice compared to Abcg2(-/-) mice. The milk-to-plasma ratio of these compounds was also higher in dairy cows carrying the ABCG2 Y581S polymorphism compared to noncarrier animals. In vitro transport assays confirmed these findings and validated differences in the bovine protein variants.
Review
Biochemistry & Molecular Biology
Samiksha Kukal, Debleena Guin, Chitra Rawat, Shivangi Bora, Manish Kumar Mishra, Priya Sharma, Priyanka Rani Paul, Neha Kanojia, Gurpreet Kaur Grewal, Shrikant Kukreti, Luciano Saso, Ritushree Kukreti
Summary: ABCG2 is a widely distributed protein in different cell types that plays a crucial role in maintaining cellular homeostasis and protecting tissues against xenobiotic insults, but its expression is influenced by various pathophysiological conditions. Therapeutic interventions should focus on regulating levels of ABCG2 rather than directly inhibiting its function to avoid serious side effects.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Methinee Pipatthana, Phurt Harnvoravongchai, Pisut Pongchaikul, Somsak Likhitrattanapisal, Matthew Phanchana, Surang Chankhamhaengdecha, Tavan Janvilisri
Summary: This study analyzed the sequence-function relationship of ABC proteins in Clostridioides difficile, revealing different protein domains associated with the ABC system and the gene organization within the genome. The classification and genetic structure of ABC transporters were identified, with a focus on the significant role of these proteins in antibiotic resistance, providing insights into the function and evolutionary relationships of ABC proteins in this pathogen.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Pharmacology & Pharmacy
Han Fu, Zhuo-Xun Wu, Zi-Ning Lei, Qiu-Xu Teng, Yuqi Yang, Charles R. Ashby, Yixiong Lei, Yuyin Lian, Zhe-Sheng Chen
Summary: This study found that Palbociclib is a substrate for the ABCB1 transporter and its in vitro anticancer efficacy is significantly decreased in cancer cells overexpressing ABCB1.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Solene Marie, Irene Hernandez-Lozano, Oliver Langer, Nicolas Tournier
Summary: Hepatocyte transporters play a crucial role in the hepatobiliary elimination of various substances, influenced by different pathophysiological conditions and specific drugs; Tc-99m-mebrofenin has been used for hepatobiliary scintigraphy for years, but the specific transporters controlling its liver kinetics have only recently been characterized; Pharmacokinetic modeling offers a translational method to study transporter-mediated drug interactions, providing insights into liver function as a biomarker.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Marine & Freshwater Biology
Hye-Min Kang, Eunjin Byeon, Haksoo Jeong, Yoseop Lee, Un-Ki Hwang, Chang-Bum Jeong, Cheolho Yoon, Jae-Seong Lee
Summary: This study examined the combined effects of nano(micro)plastics (NMPs) and arsenic (As) on the marine rotifer Brachionus plicatilis. The results showed that NPs increased As toxicity while MPs alleviated it, and NPs inhibited the efflux of As. These findings provide a deeper understanding of the environmental pollutants and their interactions.
AQUATIC TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dennis Huwald, Sabrina Duda, Raphael Gasper, Vincent Olieric, Eckhard Hofmann, Anja Hemschemeier
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Oliver Lampret, Jifu Duan, Eckhard Hofmann, Martin Winkler, Fraser A. Armstrong, Thomas Happe
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Dennis Della Corte, Hugo L. van Beek, Falk Syberg, Marcus Schallmey, Felix Tobola, Kai U. Cormann, Christine Schlicker, Philipp T. Baumann, Karin Krumbach, Sascha Sokolowsky, Connor J. Morris, Alexander Gruenberger, Eckhard Hofmann, Gunnar F. Schroeder, Jan Marienhagen
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Martin Winkler, Jifu Duan, Andreas Rutz, Christina Felbek, Lisa Scholtysek, Oliver Lampret, Jan Jaenecke, Ulf-Peter Apfel, Gianfranco Gilardi, Francesca Valetti, Vincent Fourmond, Eckhard Hofmann, Christophe Leger, Thomas Happe
Summary: The protein morphing mechanism of [FeFe]-hydrogenase CbA5H controls the reversible transition between catalytic and inactive states, with a conserved cysteine residue protecting the active site from oxygen by acting as a safety cap. This protection mechanism is regulated by three non-conserved amino acids located approximately 13 angstrom away from the active site, demonstrating remote control of the first coordination sphere chemistry of the H-cluster.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Mohamad Javad Norahan, Raphael Horvath, Nathalie Woitzik, Pierre Jouy, Florian Eigenmann, Klaus Gerwert, Carsten Koetting
Summary: Infrared spectroscopy is a powerful tool for studying protein reactions at the atomic level, with new dual-comb quantum cascade laser technology enabling rapid monitoring of reactions in the microsecond time domain. This study demonstrated the potential for high-resolution infrared spectroscopic studies in previously unexplored time regimes, paving the way for new insights into nonrepetitive biological systems such as GTPases and ATPases.
ANALYTICAL CHEMISTRY
(2021)
Article
Biology
Max-Aylmer Dreier, Philipp Althoff, Mohamad Javad Norahan, Stefan Alexander Tennigkeit, Samir F. El-Mashtoly, Mathias Luebben, Carsten Koetting, Till Rudack, Klaus Gerwert
Summary: The study demonstrates that the anion channelrhodopsin GtACR1 operates with a highly conducting C=N-anti-photocycle, without the inactivation observed in CrChR2, showing the potential for optimized optogenetic tools. The importance of the central constriction site amino acid Glu-68 in molecular gating mechanisms is highlighted, suggesting that canceling out poorly conducting photocycles could enhance channelrhodopsin efficiency for optogenetic applications.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Natascha Tomazic, Kristina E. Overkamp, Helen Wegner, Bin Gu, Florian Mahler, Marco Aras, Sandro Keller, Antonio J. Pierik, Eckhard Hofmann, Nicole Frankenberg-Dinkel
Summary: Cryptophytes use phycobiliproteins for photosynthesis, with their assembly involving PBP-lyases and substrate specificity possibly affected by a single amino acid residue.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Plant Sciences
Bernd Ackermann, Beatrix Duenschede, Bjoern Pietzenuk, Bo Hojen Justesen, Ute Kraemer, Eckhard Hofmann, Thomas Guenther Pomorski, Danja Schuenemann
Summary: Members of the Oxa1/YidC/Alb3 protein family are involved in the insertion, folding, and assembly of membrane proteins in mitochondria, bacteria, and chloroplasts. The thylakoid membrane protein Alb3 interacts with ribosomes during posttranslational insertion of specific proteins, while Alb4, a homolog located in the same membrane, does not show ribosome binding. Alb3 contacts ribosomes via its C-terminal region and at least one additional binding site within its hydrophobic core.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Francesco Tumbarello, Giampaolo Marcolin, Elisa Fresch, Eckhard Hofmann, Donatella Carbonera, Elisabetta Collini
Summary: This study investigated the energy transfer from carotenoids to chlorophylls in marine algae using two-dimensional electronic spectroscopy. Relevant details on the mechanism of excitation transfer were unveiled by comparing mutant and normal systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Abdulkadir Yayci, Nathalie Bachmann, Tim Dirks, Eckhard Hofmann, Julia E. Bandow
Summary: This study identified and characterized novel DyP-type peroxidases from Streptomyces chartreusis NRRL 3882, and found that they exhibited distinct preferences in operating conditions and stability. The findings suggest that having different DyP variants in one organism may confer evolutionary benefits. DyP-type peroxidases can be applied in biocatalysis and bioremediation.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marharyta Varatnitskaya, Julia Fasel, Alexandra Mueller, Natalie Lupilov, Yunlong Shi, Kristin Fuchs, Marco Krewing, Christoph Jung, Timo Jacob, Barbara Sitek, Julia E. Bandow, Kate S. Carroll, Eckhard Hofmann, Lars I. Leichert
Summary: This study helps to identify the residues responsible for RidA's chaperone-like function using a combination of LC-MS/MS analysis, chemo-proteomic approach, and mutagenesis study. The enhanced chaperone activity of RidA is associated with the loss of positive charges on the protein surface and increased overall protein hydrophobicity.
Article
Chemistry, Multidisciplinary
Jifu Duan, Anja Hemschemeier, David J. Burr, Sven T. Stripp, Eckhard Hofmann, Thomas Happe
Summary: In this study, the crystal structures of CN--treated [FeFe]-hydrogenase CpI from Clostridium pasteurianum were obtained, revealing that extrinsic CN- binds to the open coordination site of the cofactor. The binding of CN- leads to conformational changes in conserved residues within the proton transfer pathway, potentially facilitating efficient proton transfer. This observation provides important insights into the mechanism of CN- inhibition and proton transfer in hydrogenases.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Daniel Mann, Kristin Labudda, Sophie Zimmermann, Kai Ulrich Vocke, Raphael Gasper, Carsten Koetting, Eckhard Hofmann
Summary: This study presents time-resolved FTIR spectroscopic measurements of ATP binding and hydrolysis reaction in full-length MsbA. The reaction rates were determined and the study paves the way for investigations of allocrite transport in full-length MsbA using FTIR spectroscopy.
BIOLOGICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Claudia Brocks, Chandan K. Das, Jifu Duan, Shanika Yadav, Ulf-Peter Apfel, Subhasri Ghosh, Eckhard Hofmann, Martin Winkler, Vera Engelbrecht, Lars V. Schaefer, Thomas Happe
Summary: This study proposes a new strategy to improve the O-2 stability of [FeFe]-hydrogenases by blocking the O-2 diffusion pathway and protecting the catalytic cofactor.
Article
Chemistry, Multidisciplinary
Till Rudack, Christian Teuber, Marvin Scherlo, Joern Gueldenhaupt, Jonas Schartner, Mathias Luebben, Johann Klare, Klaus Gerwert, Carsten Koetting
Summary: Studying the Ras dimer structure has shown that inhibiting the dimerization of oncogenic Ras can suppress cancer cell growth. By incorporating unnatural amino acids into Ras and utilizing labeling methods, the accuracy of the dimer structure model can be validated.