Article
Microbiology
Courtney M. Klappenbach, Nicholas M. Negretti, Jesse Aaron, Teng-Leong Chew, Michael E. Konkel
Summary: Our study reveals that C. jejuni infection alters the size and topology of focal adhesions in epithelial cells, leading to changes in cell adhesion strength and motility. The increased phosphorylation of paxillin and altered turnover at focal adhesions may contribute to the effects on cell behavior. Additionally, inhibiting protein synthesis or using specific mutants of C. jejuni can partially reverse these effects, suggesting a potential mechanism for the observed restricted intestinal repair in infected animals.
Article
Biotechnology & Applied Microbiology
Min Pan, Xiaohui Yin, Yi-Chuan Huang
Summary: PEAK1 promotes melanoma cell growth, invasion, and metastasis by activating JAK/STAT3 signals, while PEAK1 knockdown inhibits these processes by inactivating JAK/STAT3 signals. Treatment with P6 can reverse the effects induced by PEAK1 in melanoma cells.
Article
Cell Biology
Xinyi Ren, Xiaopu Guo, Zihan Liang, Renxian Guo, Shaohui Liang, Han Liu
Summary: Cell migration is a complex process that involves the coordination of various cellular components. In this study, we discovered a novel interaction between IQGAP1 and Hax1, which plays a significant role in focal adhesion turnover and directional cell migration. Our findings suggest that Hax1 acts as a link between dynamic focal adhesions and IQGAP1, facilitating efficient cell migration. Perturbation of the IQGAP1/Hax1 interaction impairs focal adhesion turnover and inhibits directional cell migration.
CELL COMMUNICATION AND SIGNALING
(2023)
Review
Neurosciences
Sehoon Won, Katherine W. Roche
Summary: Phosphorylation regulates glutamate receptor trafficking and plays a key role in synaptic transmission and plasticity. The protein tyrosine phosphatase STEP61 is involved in differentially regulating NMDARs and AMPARs, and has been implicated in various neurological disorders. Its interplay with Fyn and GluN2B-containing NMDARs has been extensively studied, demonstrating its importance in synaptic function.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Biochemistry & Molecular Biology
Mi Kyoung Kim, Won Chan Hwang, Do Sik Min
Summary: The study reveals that the PH domain of PLD2 acts as a negative regulator of PLD2 and FAK, inhibiting the migration and invasion of tumor cells, as well as tumor formation in a xenograft mouse model.
Article
Multidisciplinary Sciences
Runchen Zhao, Siqi Cui, Zhuoxu Ge, Yuqi Zhang, Kaustav Bera, Lily Zhu, Sean X. Sun, Konstantinos Konstantopoulos
Summary: Cells transitioning from amoeboid to mesenchymal phenotypes in response to increasing hydraulic resistance exhibit oscillatory changes in actin network formation and focal adhesion maturation. Blocking the mechanosensor TRPM7 eliminates the dependence of transition period on resistance, and mathematical modeling reveals the role of intracellular calcium oscillations in this process. Hydraulic resistance emerges as a critical physical factor influencing cell phenotype, with a proposed framework for linking fluorescence signal fluctuations to morphological changes.
Article
Biochemistry & Molecular Biology
Thomas Kryza, Tashbib Khan, Scott Lovell, Brittney S. Harrington, Julia Yin, Sean Porazinski, Marina Pajic, Hannu Koistinen, Juha K. Rantala, Tobias Dreyer, Viktor Magdolen, Ute Reuning, Yaowu He, Edward W. Tate, John D. Hooper
Summary: The study identifies uPA as the master regulator of CDCP1 proteolysis, acting by directly cleaving CDCP1 and activating plasmin. Co-expression of uPA and CDCP1 predicts poor disease outcome in multiple cancers, and uPA-mediated CDCP1 proteolysis promotes metastasis in clinical models. These results suggest CDCP1 cleavage as a potential target for disrupting cancer, and establish sbABP technology as a novel approach for identifying disease-relevant proteases.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Fisheries
Junjie Wan, Xuelin Zhao, Jiqing Liu, Kaiyu Chen, Chenghua Li
Summary: In this study, a novel SFK homologue (AjSrc) was identified in Apostichopus japonicus, which mediated Vibrio splendidus-induced coelomocytes' phagocytosis via interacting with focal adhesion kinase (AjFAK) and co-phosphorylation. This study enriched the mechanism of phagocytosis in echinoderm and provided a new theoretical foundation for disease control of sea cucumber.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Cell Biology
York Posor, Charis Kampyli, Benoit Bilanges, Sushila Ganguli, Philipp A. Koch, Alexander Wallroth, Daniele Morelli, Michalina Jenkins, Samira Alliouachene, Elitza Deltcheva, Buzz Baum, Volker Haucke, Bart Vanhaesebroeck
Summary: This study uncovers a pathway by which the phosphoinositide lipid switch, phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P2), promotes the disassembly of focal adhesions through regulating the activity of RhoA-dependent stress fibers.
DEVELOPMENTAL CELL
(2022)
Article
Multidisciplinary Sciences
Takeshi Nakatani, Kohei Tsujimoto, JeongHoon Park, Tatsunori Jo, Tetsuya Kimura, Yoshitomo Hayama, Hachiro Konaka, Takayoshi Morita, Yasuhiro Kato, Masayuki Nishide, Shyohei Koyama, Shigeyuki Nada, Masato Okada, Hyota Takamatsu, Atsushi Kumanogoh
Summary: Lysosomes play a crucial role in leukocyte migration by interacting with MPRIP and enhancing myosin II-mediated actomyosin contraction, independently of mTORC1. The Ragulator complex on lysosomes is involved in this process, highlighting its importance in cell motility and immune responses.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Sandra Tavares, Nalan Liv, Milena Pasolli, Mark Opdam, Max A. K. Ratze, Manuel Saornil, Lilian M. Sluimer, Rutger C. C. Hengeveld, Robert van Es, Erik van Werkhoven, Harmjan Vos, Holger Rehmann, Boudewijn M. T. Burgering, Hendrika M. Oosterkamp, Susanne M. A. Lens, Judith Klumperman, Sabine C. Linn, Patrick W. B. Derksen
Summary: Elevated expression of non-receptor tyrosine kinase FER is associated with poor prognosis in breast cancer patients, particularly in high-grade and basal/triple-negative cases. However, high FER levels are correlated with improved outcomes after adjuvant taxane-based chemotherapy in high-risk, HER2-negative patients. Mechanistic studies reveal that FER regulates endosomal recycling and its inhibition leads to reduced breast cancer cell invasion. Furthermore, DCTN2 is identified as a substrate of FER, and its phosphorylation at tyrosine 6 is essential for tubular recycling domains formation and TNBC cell invasion.
Article
Endocrinology & Metabolism
Xiaoling Liang, Xiaoxiao Qiu, Yana Ma, Wenzhi Xu, Sijia Chen, Peipei Zhang, Mengying Liu, Xiaona Lin
Summary: Female infertility is a global issue that affects the quality of life and well-being of couples. This study focuses on the molecular mechanism of embryo implantation and the role of KRT18 in trophoblast cells. The findings demonstrate that KRT18 plays a critical role in trophoblast migration, invasion, and adhesion by binding to E-cadherin.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Adriana A. de Jesus, Guibin Chen, Dan Yang, Tomas Brdicka, Natasha M. Ruth, David Bennin, Dita Cebecauerova, Hana Malcova, Helen Freeman, Neil Martin, Karel Svojgr, Murray H. Passo, Farzana Bhuyan, Sara Alehashemi, Andre T. Rastegar, Katsiaryna Uss, Lela Kardava, Bernadette Marrero, Iris Duric, Ebun Omoyinmi, Petra Peldova, Chyi-Chia Richard Lee, David E. Kleiner, Colleen M. Hadigan, Stephen M. Hewitt, Stefania Pittaluga, Carmelo Carmona-Rivera, Katherine R. Calvo, Nirali Shah, Miroslava Balascakova, Danielle L. Fink, Radana Kotalova, Zuzana Parackova, Lucie Peterkova, Daniela Kuzilkova, Vit Campr, Lucie Sramkova, Angelique Biancotto, Stephen R. Brooks, Cameron Manes, Eric Meffre, Rebecca L. Harper, Hyesun Kuehn, Mariana J. Kaplan, Paul Brogan, Sergio D. Rosenzweig, Melinda Merchant, Zuoming Deng, Anna Huttenlocher, Susan L. Moir, Douglas B. Kuhns, Manfred Boehm, Karolina Skvarova Kramarzova, Raphaela Goldbach-Mansky
Summary: Neutrophilic inflammation is a common feature in many genetic autoinflammatory diseases. This study presents three cases of newborns with perinatal-onset of neutrophilic cutaneous small vessel vasculitis and systemic inflammation, and identified novel variants in the Src-family tyrosine kinase LYN that lead to Lyn kinase activation. The research highlights the critical role of Lyn kinase in modulating inflammatory signals, regulating microvascular permeability, neutrophil recruitment, and promoting hepatic fibrosis.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Carl G. Gahmberg, Mikaela Gronholm
Summary: Cell adhesion is crucial for organ formation, cellular migration, and interaction with target cells and the extracellular matrix. Recent research has revealed the role of integrin phosphorylations in regulating integrin activity and downstream signaling. The SARS-CoV-2 virus receptor ACE2 and potential integrin co-receptors may interact and induce phosphorylation switches and autophagy.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jason A. Estep, Lu O. Sun, Martin M. Riccomagno
Summary: Integrin Adhesion Complexes (IACs) act as links between the cytoskeleton and extracellular environment, participating in cellular motility, tissue morphogenesis, anchorage-dependent growth, and cell survival. Focal Adhesion Kinase (FAK) is a critical organizer of IAC signaling events and a genetic and therapeutic target. This study presents the design and characterization of reversible Bimolecular Complementation sensors to monitor FAK phosphorylation in living cells, providing novel means to quantify IAC signaling.