Article
Fisheries
Zhiqi Zhang, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study manufactured abundant RGNNV VLPs and developed three monoclonal antibodies that can accurately detect the capsid protein of RGNNV and show potential in detection and tracking of the virus. Two of the antibodies also possess some neutralizing capacity against RGNNV infection.
Article
Biochemistry & Molecular Biology
Huiling Xu, Guangwei Han, Ying Lu, Zehui Liu, Lina Tao, Fang He
Summary: This study produced a batch of neutralizing monoclonal antibodies against CSFV, some of which showed neutralizing reactivity against the virus. Three highly conserved linear neutralizing epitopes were identified, playing a role in virus internalization and attachment, and their prophylactic and therapeutic functions were confirmed in vivo.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Yu Nakagama, Katherine Candray, Natsuko Kaku, Yuko Komase, Maria-Virginia Rodriguez-Funes, Rhina Dominguez, Tomoya Tsuchida, Hiroyuki Kunishima, Etsuko Nagai, Eisuke Adachi, Dieudonne Mumba Ngoyi, Mari Yamasue, Kosaku Komiya, Kazufumi Hiramatsu, Naoto Uemura, Yuki Sugiura, Mayo Yasugi, Yuka Yamagishi, Hiroshige Mikamo, Satoshi Shiraishi, Takehiro Izumo, Sachie Nakagama, Chihiro Watanabe, Yuko Nitahara, Evariste Tshibangu-Kabamba, Hiroshi Kakeya, Yasutoshi Kido
Summary: The role of antibody maturation in determining the neutralizing capacity of SARS-CoV-2 was investigated. It was found that higher avidity antibodies were associated with better neutralizing capacity and cross-neutralization against variant strains.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Virology
Huan Hu, Rongfei Liu, Qianlin Li, Jin Wang, Qiang Deng, YuYing Lu, Qin Wu, Zeliang Chen, Jiahai Lu
Summary: In this study, ZIKV envelope protein domain III was expressed in E. coli to produce monoclonal antibodies. Ten monoclonal cell lines were identified, with one (mAb 8B6) showing neutralizing activity against ZIKV. Nine other mAbs could also recognize ZIKV, suggesting their potential use for ZIKV diagnosis.
Article
Medicine, Research & Experimental
Line Ledsgaard, Andreas H. Laustsen, Urska Pus, Jack Wade, Pedro Villar, Kim Boddum, Peter Slavny, Edward W. Masters, Ana S. Arias, Saioa Oscoz, Daniel T. Griffiths, Alice M. Luther, Majken Lindholm, Rachael A. Leah, Marie Sofie Moller, Hanif Ali, John McCafferty, Bruno Lomonte, Jose M. Gutierrez, Aneesh Karatt-Vellatt
Summary: This study describes the discovery and optimization of a recombinant human monoclonal immunoglobulin G antibody against alpha-cobratoxin using phage display technology. The optimized antibody showed increased neutralization potency and efficacy, providing a promising prospect for the development of recombinant antivenoms based on human monoclonal or oligoclonal antibodies.
Article
Multidisciplinary Sciences
Matthew Van Beek, Michel C. Nussenzweig, Arup K. Chakraborty
Summary: The humoral immune response relies on B cells and antibodies to provide immune protection. Memory B cells play a crucial role in reinfection or booster vaccination. Selective expansion of memory B cells with high affinity outside germinal centers results in a rapid production of optimal antibodies upon reexposure to the same antigen. Memory B cells that enter secondary germinal centers can undergo mutation and selection, leading to more potent immune responses over time. The diversity of memory B cells generated in germinal centers is important in providing protection against variant antigens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Collin Jugler, Haiyan Sun, Katherine Nguyen, Roman Palt, Mitchell Felder, Herta Steinkellner, Qiang Chen
Summary: This study describes the discovery of a new, neutralizing monoclonal antibody (mAb), 11D7, against the Wuhan-Hu-1 RBD of SARS-CoV-2. The mAb was developed into a chimeric human IgG and expressed in plants to produce a mAb with human-like glycan structure, potentially increasing its therapeutic potency and safety. 11D7 recognizes and neutralizes the Omicron variant and may provide additional synergy when used in combination with established antibody cocktails.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Immunology
Aurelien Sokal, Giovanna Barba-Spaeth, Ignacio Fernandez, Matteo Broketa, Imane Azzaoui, Andrea de La Selle, Alexis Vandenberghe, Slim Fourati, Anais Roeser, Annalisa Meola, Magali Bouvier-Alias, Etienne Crickx, Laetitia Languille, Marc Michel, Bertrand Godeau, Sebastien Gallien, Giovanna Melica, Yann Nguyen, Virginie Zarrouk, Florence Canoui-Poitrine, France Pirenne, Jerome Megret, Jean-Michel Pawlotsky, Simon Fillatreau, Pierre Bruhns, Felix A. Rey, Jean-Claude Weill, Claude-Agnes Reynaud, Pascal Chappert, Matthieu Mahevas
Summary: This study examined the MBC responses in recovered and naive individuals after mRNA vaccination against SARS-CoV-2, finding that the MBC pool in recovered individuals expanded selectively, matured further, and had potent neutralizers against VOCs. Naive individuals displayed weaker neutralizing serum responses, but a significant portion of their RBD-specific MBCs showed high affinity to multiple VOCs, suggesting potential efficient response to VOCs in naive vaccinees.
Article
Multidisciplinary Sciences
Fangzhu Zhao, Celina Keating, Gabriel Ozorowski, Namir Shaabani, Irene M. Francino-Urdaniz, Shawn Barman, Oliver Limbo, Alison Burns, Panpan Zhou, Michael J. Ricciardi, Jordan Woehl, Quoc Tran, Hannah L. Turner, Linghang Peng, Deli Huang, David Nemazee, Raiees Andrabi, Devin Sok, John R. Teijaro, Timothy A. Whitehead, Andrew B. Ward, Dennis R. Burton, Joseph G. Jardine
Summary: This study engineered three neutralizing antibodies with enhanced binding to the S protein of SARS-CoV-2 variants. The antibodies showed increased potency and breadth of neutralization against viral variants. Higher binding affinity was found to reduce the number of viral escape mutations. Studies in the Syrian hamster model demonstrated that affinity-matured antibodies provided superior protection compared to parental antibodies.
Article
Multidisciplinary Sciences
Edurne Rujas, Hong Cui, Jonathan Burnie, Clare Burn Aschner, Tiantian Zhao, Sara Insausti, Krithika Muthuraman, Anthony Semesi, Jasper Ophel, Jose L. Nieva, Michael S. Seaman, Christina Guzzo, Bebhinn Treanor, Jean-Philippe Julien
Summary: In this study, HIV-1 bNAbs were engineered by directly fusing their Fab fragments to the human apoferritin light chain, resulting in a multispecific and avid molecule. The molecule exhibited high neutralization potency against a broad panel of HIV-1 pseudoviruses and demonstrated IgG-like bioavailability in vivo through Fc receptor modulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Immunology
Rodrigo Velazquez-Moctezuma, Elias H. Augestad, Matteo Castelli, Christina Holmboe Olesen, Nicola Clementi, Massimo Clementi, Nicasio Mancini, Jannick Prentoe
Summary: Hepatitis C virus (HCV) infection causes severe liver-related diseases and leads to a significant number of deaths annually. The lack of an efficient prophylactic vaccine can be attributed to the high genetic variability and escape mutations of the virus, posing challenges to vaccine development efforts.
Article
Immunology
Maria Manali, Laura A. Bissett, Julien A. R. Amat, Nicola Logan, Sam Scott, Ellen C. Hughes, William T. Harvey, Richard Orton, Emma C. Thomson, Rory N. Gunson, Mafalda Viana, Brian Willett, Pablo R. Murcia
Summary: The breadth and potency of the antibody-mediated response against SARS-CoV-2 is influenced by exposure type, number of exposures, and the genotype of the infecting variant. Patients with mixed immunity acquired by vaccination and infection exhibit the most effective responses.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Pharmacology & Pharmacy
Jerry Li, Mina Nikanjam, Coleen K. Cunningham, Elizabeth J. McFarland, Emily E. Coates, Katherine V. Houser, Bob C. Lin, Adrian B. McDermott, Britta Flach, Lucio Gama, Richard A. Koup, Barney S. Graham, John R. Mascola, Julie E. Ledgerwood, Edmund V. Capparelli
Summary: This study used population pharmacokinetic modeling to characterize VRC01 PK for dosing selection in pediatric patients. Results showed differences in clearance and subcutaneous bioavailability of VRC01 between infants and adults, with rapid absorption observed in infants with potential therapeutic levels reached quickly.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Renato Kaylan Alves de Oliveira Franca, Jacyelle Medeiros Silva, Lucas Silva Rodrigues, Dimitri Sokolowskei, Marcelo Macedo Brigido, Andrea Queiroz Maranhao
Summary: This study utilized phage display technology to select human antibodies targeting the fusion loop region of the Zika virus. Three antibodies that can neutralize Zika virus infection were obtained through four rounds of selection. Due to the high conservation of the fusion loop region, these new antibodies have the potential to be used in therapeutic interventions against Zika virus and other flavivirus illnesses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Chunyan Yi, Caixia Su, Xiaoyu Sun, Xiao Lu, Chuanya Si, Caixuan Liu, Zhuo Yang, Hong Yuan, Yuying Huang, Jing Wen, Yonghui He, Yaguang Zhang, Liyan Ma, Yao Cong, Gan Zhao, Zhiyang Ling, Bin Wang, Bing Sun
Summary: Researchers generated 23 full-human monoclonal antibodies against the RSV fusion glycoprotein from a healthy adult with natural RSV infection. A highly potent RSV-neutralizing antibody, named 25-20, was selected, targeting a new site of vulnerability on the prefusion F protein. The optimized 25-20 showed low KD values in the picomolar range, making it an excellent candidate for passive protection against RSV infection.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Virology
Richard A. Urbanowicz, Ruixue Wang, John E. Schiel, Zhen-yong Keck, Melissa C. Kerzic, Patrick Lau, Sneha Rangarajan, Kyle J. Garagusi, Lei Tan, Johnathan D. Guest, Jonathan K. Ball, Brian G. Pierce, Roy A. Mariuzza, Steven K. H. Foung, Thomas R. Fuerst
JOURNAL OF VIROLOGY
(2019)
Article
Pharmacology & Pharmacy
Laurent Mailly, Florian Wrensch, Laura Heydmann, Catherine Fauvelle, Nicolas Brignon, Mirjam B. Zeisel, Patrick Pessaux, Zhen-yong Keck, Catherine Schuster, Thomas R. Fuerst, Steven K. H. Foung, Thomas F. Baumert
ANTIVIRAL RESEARCH
(2019)
Article
Gastroenterology & Hepatology
Florian Wrensch, Gaetan Ligat, Laura Heydmann, Catherine Schuster, Mirjam B. Zeisel, Patrick Pessaux, Francois Habersetzer, Barnabas J. King, Alexander W. Tarr, Jonathan K. Ball, Michael Winkler, Stefan Poehlmann, Zhen-yong Keck, Steven K. H. Foung, Thomas F. Baumert
Editorial Material
Gastroenterology & Hepatology
Florian Wrensch, Zhen-yong Keck, Steven K. H. Foung, Thomas F. Baumert
JOURNAL OF HEPATOLOGY
(2019)
Article
Microbiology
Zhen-Yong Keck, Brian G. Pierce, Patrick Lau, Janine Lu, Yong Wang, Alexander Underwood, Rowena A. Bull, Jannick Prentoe, Rodrigo Velazquez-Moctezuma, Melanie R. Walker, Fabio Luciani, Johnathan D. Guest, Catherine Fauvelle, Thomas F. Baumert, Jens Bukh, Andrew R. Lloyd, Steven K. H. Foung
Letter
Medicine, General & Internal
Tho D. Pham, ChunHong Huang, Oliver F. Wirz, Katharina Roltgen, Malaya K. Sahoo, Arlene Layon, Suchitra Pandey, Steven K. Foung, Scott D. Boyd, Benjamin A. Pinsky
ANNALS OF INTERNAL MEDICINE
(2020)
Article
Multidisciplinary Sciences
Netanel Tzarum, Erick Giang, Rameshwar U. Kadam, Fang Chen, Kenna Nagy, Elias H. Augestad, Rodrigo Velazquez-Moctezuma, Zhen-Yong Keck, Yuanzi Hua, Robyn L. Stanfield, Marlene Dreux, Jannick Prentoe, Steven K. H. Foung, Jens Bukh, Ian A. Wilson, Mansun Law
Article
Virology
Brian G. Pierce, Zhen-Yong Keck, Ruixue Wang, Patrick Lau, Kyle Garagusi, Khadija Elkholy, Eric A. Toth, Richard A. Urbanowicz, Johnathan D. Guest, Pragati Agnihotri, Melissa C. Kerzic, Alexander Marin, Alexander K. Andrianov, Jonathan K. Ball, Roy A. Mariuzza, Thomas R. Fuerst, Steven K. H. Foung
JOURNAL OF VIROLOGY
(2020)
Article
Biochemistry & Molecular Biology
Salman Shahid, Mingming Gao, D. Travis Gallagher, Edwin Pozharski, Robert G. Brinson, Zhen-Yong Keck, Steven K. H. Foung, Thomas R. Fuerst, Roy A. Mariuzza
Summary: This study elucidated the crystal structure of a bivalent domain-swapped Fab dimer, which recognizes the E2 envelope glycoprotein of HCV. The dimerization is mediated by the deletion of a single residue, leading to a unique Fab structure that can be used as a fiducial marker for single-particle cryoEM analysis of HCV E2. Bivalent domain-swapped Fab dimers engineered based on this structure may also serve as a means of doubling the effective size of conventional Fab-protein complexes for cryoEM.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Johnathan D. Guest, Ruixue Wang, Khadija H. Elkholy, Andrezza Chagas, Kinlin L. Chao, Thomas E. Cleveland, Young Chang Kim, Zhen-Yong Keck, Alexander Marin, Abdul S. Yunus, Roy A. Mariuzza, Alexander K. Andrianov, Eric A. Toth, Steven K. H. Foung, Brian G. Pierce, Thomas R. Fuerst
Summary: The variability of HCV poses a challenge for vaccine development, but a soluble form of E1E2 has been designed to elicit neutralizing antibodies in vivo and potentially optimize E1E2-based antigen production for future studies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Madhumati Sevvana, Zhenyong Keck, Steven K. H. Foung, Richard J. Kuhn
Summary: The molecular mechanisms of hepatitis C virus (HCV) persistence and pathogenesis are not well understood, posing challenges in designing an effective vaccine due to the virus' genetic diversity and escape mechanisms. Research on the immune response to diverse HCV variants and HCV-driven immune evasion strategies is essential for developing a universal HCV vaccine. Structural perspectives on key escape pathways mediated by mutations within epitopes, allosteric modulation, and glycan shift on envelope glycoproteins are crucial considerations for vaccine design.
CURRENT OPINION IN VIROLOGY
(2021)
Article
Gastroenterology & Hepatology
Jordan H. Salas, Richard A. Urbanowicz, Johnathan D. Guest, Nicole Frumento, Alexis Figueroa, Kaitlyn E. Clark, Zhenyong Keck, Vanessa M. Cowton, Sarah J. Cole, Arvind H. Patel, Thomas R. Fuerst, Heidi E. Drummer, Marian Major, Alexander W. Tarr, Jonathan K. Ball, Mansun Law, Brian G. Pierce, Steven K. H. Foung, Justin R. Bailey
Summary: This study describes the selection and validation of a genetically and antigenically diverse reference panel of 15 HCV pseudoparticles (HCVpps) for neutralization assays. It suggests that measuring neutralizing breadth of HCV antibodies should involve viruses spanning multiple tiers of neutralization sensitivity rather than panels selected solely for genetic diversity.
Editorial Material
Virology
Christopher Walker, James Ou, Steven Foung
CURRENT OPINION IN VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Ruixue Wang, Saori Suzuki, Johnathan D. Guest, Brigitte Heller, Maricar Almeda, Alexander K. Andrianov, Alexander Marin, Roy A. Mariuzza, Zhen-Yong Keck, Steven K. H. Foung, Abdul S. Yunus, Brian G. Pierce, Eric A. Toth, Alexander Ploss, Thomas R. Fuerst
Summary: Hepatitis C virus (HCV) is a global disease burden that requires a preventive vaccine. The study found that a vaccine based on sE1E2.LZ can induce broadly neutralizing antibodies, indicating its potential as a promising vaccine candidate for further development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Florian Wrensch, Laura Heydmann, Gaetan Ligat, Mirjam B. Zeisel, Patrick Pessaux, Francois Habersetzer, Barnabas J. King, Alexander W. Tarr, Jonathan K. Ball, Zhen-Yong Keck, Catherine Schuster, Steven Foung, Thomas F. Baumert