Article
Chemistry, Analytical
Mengze Sun, Feng Yuan, Yuliang Tang, Peng Zou, Xiaoguang Lei
Summary: In this study, we developed an APEX-CXMS method for profiling subcellular protein-protein interactions (PPIs), providing a powerful tool for understanding cellular signaling events and disease pathogenesis.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Haijun Liu, Mengru M. Zhang, Daniel A. Weisz, Ming Cheng, Himadri B. Pakrasi, Robert E. Blankenship
Summary: In cyanobacteria and red algae, the structural basis of efficient excitation energy transfer from the phycobilisome (PBS) antenna complex to the reaction centers is still unclear. A tricylindrical cyanobacterial PBS core structure was proposed using chemical cross-linking mass spectrometry and homology modeling, revealing the essential roles of anchoring domains in facilitating energy transfer to photosystem II (PSII) and PSI. The uneven bottom surface of the PBS core allows for increased accessibility for regulatory elements, such as the orange carotenoid protein, necessary for modulating photochemical activity.
Article
Biotechnology & Applied Microbiology
Longsheng Xing, Qiang Wu, Yu Xi, Cong Huang, Wanxue Liu, Fanghao Wan, Wanqiang Qian
Summary: By using PacBio single-molecule real-time sequencing, the full-length transcriptome of the codling moth was obtained, revealing a large number of novel transcripts and splice events. Additionally, long noncoding RNAs and fusion transcripts were identified and validated. This study provides important insights into the complexity of the transcriptome and will contribute to improving genome annotations and functional studies in this insect.
Article
Multidisciplinary Sciences
Yijun Fu, Kaisen Huo, Xingjie Pei, Chongjun Liang, Xinya Meng, Xiqiang Song, Jia Wang, Jun Niu
Summary: The study on Plukenetia volubilis seeds revealed differences in oil content between different types of plants, and identified several transcription factors that were significantly positively correlated with oil content. Using PacBio and Illumina platforms, non-coding RNAs and their associated genes were predicted, providing valuable references for oil biosynthesis and genetic improvement in Plukenetia volubilis.
Article
Multidisciplinary Sciences
Laura Secondulfo, Melissa T. Hooijmans, Joep J. Suskens, Valentina Mazzoli, Mario Maas, Johannes L. Tol, Aart J. Nederveen, Gustav J. Strijkers
Summary: This study developed a DTI-based method for quantitatively assessing fiber angles and changes in leg muscles, allowing for longitudinal studies on muscle fiber architectural adaptations in healthy individuals. The results showed that the method was feasible and repeatable, and it was able to detect changes in lower-leg muscle fiber angles with varying ankle angles.
Article
Biochemistry & Molecular Biology
Mingyue Bao, Xue Wang, Ruyi Sun, Zhiqiang Wang, Jiqian Li, Tinglei Jiang, Aiqing Lin, Hui Wang, Jiang Feng
Summary: In this study, the full-length transcriptome of the Great Himalayan Leaf-nosed bat was analyzed for the first time using PacBio single-molecule real-time sequencing. Numerous alternative splicing and alternative polyadenylation events were detected, along with the identification of new genes and isoforms. Key novel genes associated with nervous, signal transduction, and immune system processes were also identified. These results provide an optimized and updated reference resource for the H. armiger genome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Haiyang Duan, Jianxin Li, Yan Sun, Xuehang Xiong, Li Sun, Wenlong Li, Jionghao Gao, Na Li, Junli Zhang, Jiangkuan Cui, Zhiyuan Fu, Xuehai Zhang, Jihua Tang
Summary: By scoring three leaf angle-related traits on 492 maize inbred lines, we found that leaf angle-related traits were normally distributed and showed significant variation among environments and genotypes. A genome-wide association study identified 85 significant SNPs and 32 non-redundant QTLs that explained 4.80%-24.09% of the phenotypic variation. Meta-analysis identified 47 meta-QTLs and 816 genes, as well as co-located QTLs discovered by both GWAS and meta-analysis.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Cong Gao, Lin Zhang, Yunchen Xu, Yue Liu, Xiao Xiao, Liu Cui, Yiping Xia, Yun Wu, Ziming Ren
Summary: In this study, high-quality transcripts of Lily and its variety during bulblet initiation were obtained using Pacific Biosciences (PacBio) full-length sequencing and Illumina RNA sequencing. The results showed that carbohydrate metabolism and auxin signaling pathways are coordinately regulated during bulblet initiation, providing a basis for further research on the molecular mechanism of bulblet initiation in lily and other bulbous flowers.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biophysics
Stefan Papenkort, Markus Boel, Tobias Siebert
Summary: The study aimed to develop an architectural model capable of predicting the complete 3D fascicle architecture for primarily unipennate muscles of an arbitrary age. Experimental results showed increases in plantaris muscle belly length, mean fascicle length, and mean pennation angle during maturation. The model delivered good results matching interindividual differences and could be utilized for future simulation studies.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Benny Danilo Belviso, Giuseppe Felice Mangiatordi, Domenico Alberga, Vincenzo Mangini, Benedetta Carrozzini, Rocco Caliandro
Summary: The structure of rituximab, the first monoclonal antibody developed to target CD20 protein, has been investigated using small-angle X-ray scattering (SAXS), which revealed an asymmetric average conformation in solution. The flexibility of the antibody was found to depend on the relative orientations of its domains, with one of the Fab arms playing a major role. The structural characterization of rituximab can aid in the design of next-generation anti-CD20 antibodies and the optimization of its production.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Plant Sciences
Yaozong Shen, Xiao Han, Hui Wang, Kai Fan, Yu Wang, Jiazhi Shen, He Li, Dapeng Song, Shibo Ding, Zhaotang Ding
Summary: This study investigated the effect of light quality on callus formation in tea cuttings, and revealed the molecular mechanism behind the regulation of callus formation by light quality through phytohormone determination and full-length transcriptome sequencing.
PLANT GROWTH REGULATION
(2023)
Article
Plant Sciences
Yaozong Shen, Xiao Han, Hui Wang, Kai Fan, Yu Wang, Jiazhi Shen, He Li, Dapeng Song, Shibo Ding, Zhaotang Ding
Summary: Shortcutting is the main method of tea propagation. This study investigated the effect of light quality on the callus formation at the base of tea cuttings. The results showed that blue light (BL) promoted callus formation while red light (RL) inhibited callus formation. Phytohormone determination and full-length transcriptome sequencing revealed the molecular mechanism of light quality regulating the callus formation of tea cuttings.
PLANT GROWTH REGULATION
(2023)
Article
Anatomy & Morphology
Saul Martin-Rodriguez, Juan Jose Gonzalez-Henriquez, Victor Galvan-Alvarez, Sara Cruz-Ramirez, Jose A. Calbet, Joaquin Sanchis-Moysi
Summary: The study found that there are morphological differences in the tibialis anterior muscle between males and females, with males having greater muscle thickness and pennation angle compared to females, while fascicle length is similar in both sexes. Additionally, muscle thickness and pennation angle are greater in the deep unipennate region compared to the superficial region, and these differences remain significant even after accounting for leg lean mass and shank length. There are sex differences in muscle thickness and pennation angle in the superficial region, but not in the deep region, and adjusted fascicle length is longer in females than males when considering leg lean mass and shank length.
JOURNAL OF ANATOMY
(2023)
Article
Plant Sciences
Kamrun Nahar, William Bovill, Glenn McDonald
Summary: Improving the uptake efficiency of wheat is crucial for enhancing phosphorus recovery and efficiency. This study investigated the root traits of 10 wheat varieties to identify which traits contribute to the variation in phosphorus efficiency. The results showed that rhizosheath volume and root hair length were the most consistently related root traits to yield response and phosphorus efficiency in the field trials.
JOURNAL OF PLANT NUTRITION
(2022)
Article
Multidisciplinary Sciences
Brent J. Raiteri, Leon Lauret, Daniel Hahn
Summary: This study aimed to estimate the force-length relation of the tibialis anterior (TA) muscle. The results showed that TA produced its maximum active force at 15° dorsiflexion, and its force-length curve agreed with a sarcomere model within the muscle. These findings are important for predicting forces during movement.
Article
Chemistry, Multidisciplinary
Bryan E. Barton, Michael J. Behr, Jasson T. Patton, Eric J. Hukkanen, Brian G. Landes, Weijun Wang, Nicholas Horstman, James E. Rix, Denis Keane, Steven Weigand, Mark Spalding, Chris Derstine
Article
Chemistry, Physical
Amer Deirieh, Irene Y. Chang, Michael L. Whittaker, Steven Weigand, Denis Keane, James Rix, John T. Germaine, Derk Joester, Peter B. Flemings
APPLIED CLAY SCIENCE
(2018)
Article
Materials Science, Characterization & Testing
P. Gelineau, S. Weigand, L. Cauvin, F. Bedoui
Article
Biochemical Research Methods
Ekaterina V. Filippova, Steven Weigand, Olga Kiryukhina, Alan J. Wolfe, Wayne F. Anderson
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2019)
Article
Polymer Science
Benhui Fan, Zhaoliang Xing, Fahmi Bedoui, Jinkai Yuan, Xiaoxin Lu, Delong He, Mingyu Zhou, Chong Zhang, Zhimin Dang, Steven Weigand, Jinbo Bai
Article
Engineering, Chemical
Adote S. Blivi, Fahmi Bedoui, Steven Weigand, Djimedo Kondo
POLYMER ENGINEERING AND SCIENCE
(2020)
Article
Chemistry, Multidisciplinary
Zachary J. Urbach, Sarah S. Park, Steven L. Weigand, James E. Rix, Byeongdu Lee, Chad A. Mirkin
Summary: The study demonstrates that in a magnetic field, cubic Fe3O4 nanoparticles functionalized with DNA can exhibit unexpected crystallographic alignment of the entire superlattice, expanding the potential of crystal engineering with DNA through physical and chemical control.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Naneki C. McCallum, Florencia A. Son, Tristan D. Clemons, Steven J. Weigand, Karthikeyan Gnanasekaran, Claudia Battistella, Brooke E. Barnes, Hashanthi Abeyratne-Perera, Zofia E. Siwicka, Christopher J. Forman, Xuhao Zhou, Martin H. Moore, Daniel A. Savin, Samuel Stupp, Zheng Wang, Gary J. Vora, Brandy J. Johnson, Omar K. Farha, Nathan C. Gianneschi
Summary: Research on artificial allomelanin analogues reveals high intrinsic microporosity and high BET surface area achievable through a simplified method. These synthetic allomelanin particles exhibit functions such as adsorbing ammonia and nerve agent simulants, as well as preventing agent breakthrough on breathable fabrics. Compared to naturally derived fungal melanin, synthetic analogues have higher porosity.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Aaron P. Lindsay, Ashish Jayaraman, Austin J. Peterson, Andreas J. Mueller, Steven Weigand, Kristoffer Almdal, Mahesh K. Mahanthappa, Timothy P. Lodge, Frank S. Bates
Summary: The reanalysis of an asymmetric PEP-PDMS diblock co-polymer revealed a rich phase behavior including a dodecagonal quasicrystal, a Frank-Kasper sigma phase, and a body-centered cubic packing. When subjected to large amplitude oscillatory shear below the order-order transition temperature, a twinned BCC phase emerged, followed by a transition to an anisotropic DDQC state upon heating. Surprisingly, no evidence of epitaxy was observed during the sigma-BCC transition, suggesting a shear-induced order-order transition and an apparent BCC-DDQC epitaxy favored by micelle translation-mediated ordering dynamics far below the order-order transition temperature.
Article
Polymer Science
Jacob S. A. Ishibashi, Ian C. Pierce, Alice B. Chang, Aristotelis Zografos, Bassil M. El-Zaatari, Yan Fang, Steven J. Weigand, Frank S. Bates, Julia A. Kalow
Summary: The composition of low-T-g n-butylacrylate-block-(acetoxyaceto)-ethyl acrylate block polymers is investigated as a strategy to tune the properties of dynamically cross-linked vinylogous urethane vitrimers. Increasing the proportion of the cross-linkable block results in changes in thermorheological properties, structure, and stress relaxation that cannot be solely explained by increasing cross-link density. The thermomechanical and viscoelastic properties of block-copolymer-derived vitrimers are determined by the subtle interplay of microphase separation and network defects.
Article
Chemistry, Multidisciplinary
Adam J. Dannenhoffer, Hiroaki Sai, Boris Harutyunyan, Ashwin Narayanan, Natalia E. Powers-Riggs, Alexandra N. Edelbrock, James Passarelli, Steven J. Weigand, Michael R. Wasielewski, Michael J. Bedzyk, Liam C. Palmer, Samuel Stupp
Summary: The thermal annealing in high ionic strength aqueous environments can induce the formation of extra-large ribbon-shaped crystalline supramolecular polymers from perylene monoimide chromophore assemblies, which are highly porogenic and show enhanced photocatalytic hydrogen production. This growth process was monitored by absorbance spectroscopy, X-ray scattering, and microscopy.
Article
Multidisciplinary Sciences
Z. Alvarez, A. N. Kolberg-Edelbrock, I. R. Sasselli, J. A. Ortega, R. Qiu, Z. Syrgiannis, P. A. Mirau, F. Chen, S. M. Chin, S. Weigand, E. Kiskinis, S. Stupp
Summary: Researchers have successfully utilized peptide amphiphile supramolecular polymers containing two distinct signals to achieve cell signaling and tissue regeneration in a mouse model of severe spinal cord injury. By mutating the peptide sequence of the monomers, they intensified the motions of molecules within scaffold fibrils, resulting in notable effects such as vascular growth, axonal regeneration, and functional recovery. The study suggests that optimizing cell signaling through ensembles of molecules could be achieved by tuning their internal motions.
Article
Polymer Science
Huiqun Wang, Sebla Onbulak, Steven Weigand, Frank S. Bates, Marc A. Hillmyer
Summary: The researchers synthesized a series of polyethylene-graft-aPP graft copolymers and found that increasing aPP content led to decreased melting temperatures and glass transition temperatures of PE, as well as decreased crystallinity. Additionally, the linear viscoelastic behavior of the PE-g-aPP graft copolymers exhibited greatly enhanced dynamic modulus and complex viscosity, with the effect increasing as side chain number and/or length increased.
Meeting Abstract
Chemistry, Multidisciplinary
Thomas C. Fitzgibbons, Larisa Reyes, Michael Petr, Bill Winniford, Michelle Mejia, Brian Landes, Peter Eastman, Catheryn Jackson, Steven Weigand, James Rix
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2018)
Meeting Abstract
Chemistry, Multidisciplinary
A. Kannan, David Bucknall, Aaron Eberle, Timothy Shaffer, Alexander Norman, S. Weigand
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2016)