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Biochemistry & Molecular Biology
Eva Wisniewski, Domonkos Czaran, Fanni Kovacs, Eniko Bahurek, Afrodite Nemeth, Peter Sasvari, Gergo Szanda, Aladar Pettko-Szandtner, Eva Klement, Erzsebet Ligeti, Roland Csepanyi-Komi
Summary: This study reveals the regulatory role of different phosphorylation patterns on the GAP activity, superoxide production, and phagocytic effect of ARHGAP25.
Article
Immunology
Kirsti Hornigold, Martin J. Baker, Polly A. Machin, Stephen A. Chetwynd, Anna-Karin Johnsson, Chiara Pantarelli, Priota Islam, Melanie Stammers, Laraine Crossland, David Oxley, Hanneke Okkenhaug, Simon Walker, Rachael Walker, Anne Segonds-Pichon, Yoshinori Fukui, Angeliki Malliri, Heidi C. E. Welch
Summary: Tiam1 is a novel regulator of Rac-dependent neutrophil responses, controlling adhesion, migration, reactive oxygen species generation, degranulation, NETs release, and bacterial killing. It functions differently from other Rac-GEFs by restricting neutrophil adhesion and beta 2 integrin activity, and by promoting the expression of regulators of small GTPases and cytoskeletal dynamics.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fan Zhang, Hong Fang, Min Wang, Feng He, Hui Tao, Ruyi Wang, Jiawei Long, Jiyang Wang, Guo-Liang Wang, Yuese Ning
Summary: APIP5 plays dual roles in plants, acting as a transcription factor to regulate gene expression in the nucleus and as an RNA-binding protein to regulate mRNA turnover in the cytoplasm, a previously unidentified regulatory mechanism of plant transcription factors at the transcriptional and post-transcriptional levels.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Johannes W. P. Kuiper, Julia Krause, Leon Potgeter, Jonas Adrian, Christof R. Hauck
Summary: A genome-wide CRISPR/Cas9 screen identified CYRI-B as a negative regulator of CEACAM3-mediated phagocytosis. Knockout of CYRI-B resulted in enhanced CEACAM3 downstream signaling and increased phagocytosis of bacteria. These findings shed light on the mechanism of opsonin-independent phagocytosis mediated by CEACAM3 and highlight the negative regulatory role of CYRI-B.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Huanhuan Wang, Yukie Yamahashi, Marcel Riedl, Mutsuki Amano, Kozo Kaibuchi
Summary: The small GTPase Rac1 plays a critical role in cellular processes and cognitive functions. Inhibiting RacGAPs to sustain Rac1 activation shows potential as a therapeutic strategy for improving Alzheimer's disease-related learning deficits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Takeshi Honda, Yusuke Nishio, Hiroki Sakai, Masataka Asagiri, Kiyoshi Yoshimura, Makoto Inui, Atsuo Kuramasu
Summary: Our study reveals that calcium regulates the PI3K-dependent activation of Rac GTPases through calmodulin, mediating histamine-induced chemotaxis of mast cells. Rac GTPases and Akt play independent roles in this process.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Yifan Zhang, Ziyue Chen, Fang Wang, Honghua Sun, Xueliang Zhu, Jianping Ding, Tianlong Zhang
Summary: This study demonstrates that Rab9 utilizes Nde1 as an effector to interact with the dynein motor complex, thereby tethering the late endosomes to the dynein motor for their retrograde transport to the TGN.
Article
Cell Biology
Elizabeth B. Thomas, Renaldo Sutanto, Richard S. Johnson, Han-Wei Shih, Germain C. M. Alas, Jana Krtkova, Michael J. MacCoss, Alexander R. Paredez
Summary: A proposed staging system for encysting Giardia based on CWP1 staining of ESVs, along with molecular distinctiveness of maturing ESVs through tracking GlRab GTPases, and the relationship between GlRac and ESV stages. Additionally, potential interactors of GlRac identified through proteomic studies could serve as additional markers for ESV stages.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Grace Velez Crespo, Jescelica Ortiz, Eliud Hernandez O'Farrill, Cornelis P. Vlaar, Mikhail Inyushin, Yuriy Kucheryavykh, Lilia Kucheryavykh
Summary: This study evaluated the effectiveness of HV-107, a derivative of Ehop-016, in inhibiting metastasis in triple-negative breast cancer (TNBC) at lower doses. The results demonstrated that HV-107 effectively inhibited Rac activity, reduced cell invasion and invadopodia formation, and showed potential in inhibiting tumor metastasis in a mouse model. Therefore, HV-107 may be a promising therapeutic medication for addressing TNBC metastasis.
MOLECULAR MEDICINE
(2023)
Article
Fisheries
Lilin Hou, Xue Qiao, Youjing Li, Yuhao Jin, Ranyang Liu, Sicong Wang, Kai Zhou, Lingling Wang, Linsheng Song
Summary: In this study, a novel AKT homologue, CgAKT1, was identified from the Pacific oyster, and it was found to play a negative role in the antiviral immunity of oysters by regulating the synthesis of CgIFNLP.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Duo Yang, Haiyan Zhou, Jiwu Lin, Shuangxi Zhao, Hao Zhou, Zhaochu Yin, Bin Ni, Yong Chen, Wanqin Xie
Summary: A novel heterozygous variation c.1871A>G (p.Lys624Arg) has been identified in the SIPA1L3 gene in a three-generation Chinese family with isolated juvenile-onset cataracts. The Lys 624 residue of SIPA1L3 is conserved across species and may have an impact on the regulatory function of SIPA1L3 on Rap signaling. Further investigations are needed to validate the functional alteration of this variant.
FRONTIERS IN GENETICS
(2021)
Article
Plant Sciences
Xiaobing Kou, Peng Cao, Qianke He, Peng Wang, Shaoling Zhang, Juyou Wu
Summary: Pollen tube growth is crucial for sexual reproduction in flowering plants, and Catharanthus roseus receptor-like kinases (CrRLK1L) play a significant role in this process. A CrRLK1L protein, PbrCrRLK1L13, was identified in pear (Pyrus bretschneideri) and found to be essential for normal pollen tube tip growth. Knocking down PbrCrRLK1L13 resulted in faster pollen tube growth. Further analysis revealed that the kinase domain of PbrCrRLK1L13 interacts with the C-terminal region of PbrGEF8, and PbrCrRLK1L13 activates the phosphorylation of PbrGEF8 in vitro. Additionally, PbrROP1 and PbrROP2 were identified as downstream targets of the PbrCrRLK1L13-PbrGEF8 signaling pathway. Disrupting the expression of PbrCrRLK1L13, PbrGEF8, or PbrROP1/2 led to an imbalance in cellulose deposition in the pollen tube wall. Based on these findings, a model was proposed to elucidate the signaling events regulating pear pollen tube growth, in which PbrCrRLK1L13 acts as a surface regulator of the PbrROP1 and PbrROP2 signaling pathway via PbrGEF8, thereby influencing cellulose deposition and regulating pear pollen tube growth.
HORTICULTURE RESEARCH
(2022)
Article
Cell Biology
Aparajita Lahree, Sara de Jesus Santos Baptista, Sofia Marques, Veronika Perschin, Vanessa Zuzarte-Luis, Manisha Goel, Hadi Hasan Choudhary, Satish Mishra, Christian Stigloher, Marino Zerial, Varadharajan Sundaramurthy, Maria M. Mota
Summary: Intracellular pathogens manipulate host cells to survive and thrive. This study shows that liver-stage Plasmodium parasites compete with the host to sequester a host endosomal-adaptor protein (APPL1) involved in regulating endocytosis signaling. There is a correlation between the level of APPL1 enrichment at the parasitophorous vacuole membrane (PVM) and parasite size during exoerythrocytic development.
Article
Cell Biology
Anshu Bhattacharya, Rukmini Mukherjee, Santosh Kumar Kuncha, Melinda Elaine Brunstein, Rajeshwari Rathore, Stephan Junek, Christian Muench, Ivan Dikic
Summary: Acute lysosomal membrane damage reduces functional lysosomes, but they have a recovery potential independent of lysosomal biogenesis and remain unaffected in cells depleted in TFEB and TFE3. A lysosomal membrane regeneration pathway was discovered that depends on ATG8, LIMP2, TBC1D15, dynamin-2, kinesin-5B, and clathrin. LIMP2 acts as a lysophagy receptor, binding ATG8, which recruits TBC1D15 to damaged lysosomal membranes. TBC1D15 interacts with ATG8 proteins and stabilizes the autophagic lysosomal reformation machinery, promoting lysosomal tubule formation and dynamin-2-dependent scission.
NATURE CELL BIOLOGY
(2023)
Article
Oncology
Bo Jia, Yuran Jiang, Yu Huan, Yu Han, Wei Liu, Xiao Liu, Yingwen Wang, Zhengcong Cao, Kuo Zhang, Jintao Gu, Qingdong Guo, Zhou Fei
Summary: Transcriptomic analysis reveals that RACGAP1 is highly expressed in glioma and promotes cell proliferation through regulating MCM3 expression. Inhibition of RACGAP1 prolongs the survival time of glioma mice. Targeting RACGAP1 using T7-exo as a delivery system significantly improves the survival time of glioma bearing mice.
TRANSLATIONAL ONCOLOGY
(2023)