Article
Microbiology
Angela Rumaseb, Roberto R. Moraes Barros, Juliana M. Sa, Jonathan J. Juliano, Timothy William, Kamil A. Braima, Bridget E. Barber, Nicholas M. Anstey, Ric N. Price, Matthew J. Grigg, Jutta Marfurt, Sarah Auburn
Summary: Increasing reports of resistance to chloroquine in Plasmodium vivax raise concerns for its elimination. The lack of an effective marker for chloroquine resistance greatly hinders surveillance. This study examines the genetic basis of chloroquine resistance in Malaysian P. vivax isolates, and suggests that the proposed markers, MS334 and In9pvcrt, are not reliable indicators of treatment efficacy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biology
Joao L. Silva-Filho, Joao C. K. Dos-Santos, Carla Judice, Dario Beraldi, Kannan Venugopal, Diogenes Lima, Helder Nakaya, Erich De Paula, Stefanie C. P. Lopes, Marcus V. G. Lacerda, Matthias Marti, Fabio T. M. Costa
Summary: In patients with vivax malaria, there is a correlation between peripheral and total parasite biomass and host response, with high heterogeneity in clinical features, parasite burden, and host signatures. Patients can be categorized into Vivax(low) and Vivax(high) clusters based on differences in total parasite biomass, rather than peripheral parasitaemia, showing distinct alterations in various parameters related to host homeostasis.
Article
Medicine, Research & Experimental
Marly M. Melo, Monica R. F. Costa, Franklin S. Santana Filho, Jose Diego Brito-Sousa, Anne C. G. Almeida, Wuelton M. Monteiro, Gisely C. Melo, Jose Luiz Fernandes Vieira, Maria das Gracas Costa Alecrim
Summary: This study described the pharmacokinetic parameters of chloroquine in whole blood of adult patients with P. vivax malaria from the western Brazilian Amazon basin and compared the area under the curve (AUC) with the parasitological outcome at day 28. The results showed that the pharmacokinetic parameters were similar in both groups, suggesting the lack of influence of early reappearance of parasites on chloroquine pharmacokinetics.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Infectious Diseases
Anand Odedra, Kari Mudie, Glen Kennedy, Rebecca E. Watts, Emilie Rossignol, Hayley Mitchell, Jeremy Gower, Maria Rebelo, Zuleima Pava, Rebecca Pawliw, Stephen Woolley, David G. Lalloo, Greg Robinson, Sean Lynch, Katharine A. Collins, Fiona Amante, James McCarthy
Summary: The study assessed the safety and effectiveness of using apheresis for extracting and concentrating P. vivax parasites, finding that apheresis can concentrate asexual parasites and gametocytes, but the concentration level is modest and may not be ideal for harvesting P. vivax.
Review
Immunology
Erica M. Pasini, Clemens H. M. Kocken
Summary: This article reviews the modeling of relapsing parasites Plasmodium vivax and Plasmodium ovale in non-human primates, highlighting the contribution of these models to our current understanding of the factors that govern parasite-host interactions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Usheer Kanjee, Christof Gruring, Prasad Babar, Anosha Meyers, Rashmi Dash, Ligia Pereira, Anjali Mascarenhas, Mudit Chaand, Gabriel W. Rangel, Martha A. Clark, Laura Chery, Edwin Gomes, Pradipsinh K. Rathod, Manoj T. Duraisingh
Summary: Plasmodium vivax utilizes two invasion pathways, DARC and TfR1, with significant variation in receptor usage among strains, suggesting alternative invasion pathways similar to Plasmodium falciparum, impacting pathogenesis and vaccine development.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Cindy S. Chu, James A. Watson, Aung Pyae Phyo, Htun Htun Win, Widi Yotyingaphiram, Suradet Thinraow, Nay Lin Soe, Aye Aye Aung, Pornpimon Wilaisrisak, Kanokpich Kraft, Mallika Imwong, Warunee Hanpithakpong, Daniel Blessborn, Joel Tarning, Stephane Proux, Clare Ling, Francois H. Nosten, Nicholas J. White
Summary: Young children have lower exposure to primaquine and carboxyprimaquine as well as lower levels of methemoglobinemia compared to adults, suggesting they may need higher weight-adjusted primaquine doses than adults.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Myat Thu Soe, Pyae Linn Aung, Myat Htut Nyunt, Myint Myint Sein, Cho Cho, Zhaoqing Yang, Lynette Menezes, Daniel M. Parker, Myat Phone Kyaw, Liwang Cui
Summary: This study investigates the efficacy of chloroquine for treating uncomplicated P. vivax malaria in the eastern and western borders of Myanmar and reveals geographical heterogeneity in treatment efficacy.
Article
Infectious Diseases
Hongyun Ding, Ying Dong, Yan Deng, Yanchun Xu, Yan Liu, Jing Wu, Mengni Chen, Canglin Zhang, Li Liu, Yingkun Lin
Summary: This study molecularly monitored the chloroquine resistance in Plasmodium vivax strains from vivax malaria patients in Yunnan Province, focusing on the analysis of polymorphism in the P. vivax chloroquine resistance transporter protein orthologous gene (pvcrt-o). The study found the presence of P. vivax strains with a K10 insertion and partial sequence deletions in the promoter region of the pvcrt-o gene.
Article
Parasitology
Jutta Marfurt, Grennady Wirjanata, Pak Prayoga, Ferryanto Chalfein, Leo Leonardo, Boni F. Sebayang, Dwi Apriyanti, Maic A. E. M. Sihombing, Leily Trianty, Rossarin Suwanarusk, Alan Brockman, Kim A. Piera, Irene Luo, Angela Rumaseb, Barbara MacHunter, Sarah Auburn, Nicholas M. Anstey, Enny Kenangalem, Rintis Noviyanti, Bruce Russell, Jeanne R. Poespoprodjo, Ric N. Price
Summary: Drug resistant Plasmodium parasites pose a major threat to malaria control. This study in Indonesia found high levels of multidrug resistant parasites, leading to a change in national treatment policy. After 12 years of intense drug pressure, while chloroquine resistance appears sustained, there has been a slight decline in piperaquine susceptibility, although not reaching clinically significant levels.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2021)
Article
Immunology
Kayla Sylvester, Steven P. Maher, Dora Posfai, Michael K. Tran, McKenna C. Crawford, Amelie Vantaux, Benoit Witkowski, Dennis E. Kyle, Emily R. Derbyshire
Summary: This study utilizes high-resolution microscopy to characterize temporal changes of the TVN in Plasmodium vivax liver stage, revealing its presence in dormant hypnozoites and association with host nuclei. The unexpected association of the host water channel protein AQP3 with TVN-derived vesicles is demonstrated, suggesting a potential function of AQP3 during this stage. These findings enhance understanding of host-parasite interactions in both dormant and replicating P. vivax liver stage forms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Anne Cristine Almeida, Anna Beatriz Ribeiro Elias, Maria Paula Marques, Gisely Cardoso de Melo, Allyson Guimaraes da Costa, Erick Frota Gomes Figueiredo, Larissa Wanderley Brasil, Fernanda Rodrigues-Soares, Wuelton Marcelo Monteiro, Marcus Vinicius Guimaraes de Lacerda, Vera Lucia Lanchote, Guilherme Suarez-Kurtz
Summary: The study investigated the impact of Plasmodium vivax malaria and chloroquine-primaquine chemotherapy on CYP2D6 and CYP2C19 activity in patients from the Brazilian Amazon. Results showed alterations in CYP2D6 and CYP2C19 metabolic phenotypes, potentially due to changes in pharmacokinetics and cytokine levels during different stages of vivax malaria illness and treatment.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2021)
Article
Immunology
Jie Bai, Fei Liu, Fan Yang, Yan Zhao, Xitong Jia, Sataporn Thongpoon, Wanlapa Roobsoog, Jetsumon Sattabongkot, Li Zheng, Zeshi Cui, Wenqi Zheng, Liwang Cui, Yaming Cao
Summary: The potential of Pv22 as a transmission-blocking vaccine (TBV) candidate was evaluated by expressing it in transgenic Plasmodium berghei parasites. The study showed that Pv22 has weaker TB activity, suggesting it may not be a promising TBV candidate for Plasmodium vivax.
Review
Microbiology
Carola Schafer, Gigliola Zanghi, Ashley M. Vaughan, Stefan H. I. Kappe
Summary: Plasmodium vivax, the most widespread human malaria parasite, can form latent liver stages known as hypnozoites which can activate weeks, months, or even years after the primary infection, causing relapses of blood stage infection. Eliminating hypnozoites is a major obstacle for malaria treatment and eradication as they are undetectable and unaffected by most antimalarial drugs.
ANNUAL REVIEW OF MICROBIOLOGY, VOL 75, 2021
(2021)
Article
Multidisciplinary Sciences
Sittinont Chainarin, Ubonwan Jaihan, Parsakorn Tapaopong, Pinyapat Kongngen, Nawapol Kunkeaw, Liwang Cui, Jetsumon Sattabongkot, Wang Nguitragool, Wanlapa Roobsoong
Summary: The role of EphA2 in Plasmodium vivax infection was investigated, and it was found that EphA2 enhances the parasite infection rate. A new cell line expressing the whole extracellular domain of EphA2 was generated, which supports a higher rate of P. vivax infection and is a valuable tool for P. vivax liver-stage research.
SCIENTIFIC REPORTS
(2022)
Article
Public, Environmental & Occupational Health
Nicholas M. Douglas, Kim A. Piera, Angela Rumaseb, Benedikt Ley, Nicholas M. Anstey, Ric N. Price
Summary: This article describes a case of severe hemolysis occurring in a G6PD-deficient Australian man after using primaquine for prophylaxis, without malaria. The hemolysis resulted in renal tubular and glomerular injury, and the patient showed elevated levels of urinary beta-2-microglobulin and glycocalyx metabolites. The study also found that regular dose of paracetamol can be safely used in cases of severe oxidative hemolysis.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2023)
Article
Microbiology
Angela Rumaseb, Roberto R. Moraes Barros, Juliana M. Sa, Jonathan J. Juliano, Timothy William, Kamil A. Braima, Bridget E. Barber, Nicholas M. Anstey, Ric N. Price, Matthew J. Grigg, Jutta Marfurt, Sarah Auburn
Summary: Increasing reports of resistance to chloroquine in Plasmodium vivax raise concerns for its elimination. The lack of an effective marker for chloroquine resistance greatly hinders surveillance. This study examines the genetic basis of chloroquine resistance in Malaysian P. vivax isolates, and suggests that the proposed markers, MS334 and In9pvcrt, are not reliable indicators of treatment efficacy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Kamala Thriemer, Tamiru Shibiru Degaga, Mohammad Shafiul Alam, Bipin Adhikari, Rupam Tripura, Mohammad Sharif Hossain, Michael Christian, Najia K. Ghanchi, Hellen Mnjala, Sophie Weston, Benedikt Ley, Angela Rumaseb, Dagimawie Tadesse, Tedla Teferi, Daniel Yilma, Grant Lee, Holger Unger, Inge Sutanto, Ayodhia Pitaloka Pasaribu, Prakash Ghimire, Mohammad Asim Beg, Ric N. Price
Summary: The COVID-19 pandemic has had a significant impact on global clinical trials, forcing us to adapt our working model. We implemented virtual training, remote monitoring, and established a virtual network to enhance collaboration. These adaptations have brought unexpected advantages, including improved engagement, reduced carbon footprint, and cost savings.
Article
Public, Environmental & Occupational Health
Kinley Penjor, Ugyen Zangpo, Dorji Tshering, Benedikt Ley, Ric N. Price, Kinley Wangdi
JOURNAL OF TRAVEL MEDICINE
(2023)
Article
Infectious Diseases
Elke Wynberg, Robert J. Commons, Georgina Humphreys, Hazel Ashurst, Rebekah Burrow, George O. Adjei, Martin Adjuik, Nicholas M. Anstey, Anup Anvikar, Kevin J. Baird, Bridget E. Barber, Hubert Barennes, Elisabeth Baudin, David J. Bell, Delia Bethell, Tran Quang Binh, Isabelle Borghini, Cindy S. Chu, Andre Daher, Umberto D'Alessandro, Debashish Das, Timothy Me Davis, Peter J. de Vries, Abdoulaye A. Djimde, Arjen M. Dondorp, Grant Dorsey, Jean-Francois F. Faucher, Carole Fogg, Oumar Gaye, Matthew Grigg, Christoph Hatz, Piet A. Kager, Marcus Lacerda, Moses Laman, Andreas Martensson, Herve Ignace Eby Menan, Wuelton M. Monteiro, Brioni R. Moore, Francois Nosten, Bernhards Ogutu, Lyda Osorio, Louis K. Penali, Dhelio B. Pereira, Awab G. Rahim, Michael Ramharter, Issaka Sagara, Birgit Schramm, Lorenz Seidlein, Andre M. Siqueira, Sodiomon B. Sirima, Peter Starzengruber, Inge Sutanto, Walter R. Taylor, Offianan A. Toure, Jurg Utzinger, Innocent Valea, Giovanni Valentini, Nicholas J. White, Timothy William, Charles J. Woodrow, Caitlin L. Richmond, Philippe J. Guerin, Ric N. Price, Kasia Stepniewska
Summary: This study describes the variability in white blood cell count during acute uncomplicated malaria and estimates the impact of using an assumed value of white blood cell count on estimates of parasite density and clearance.
Article
Public, Environmental & Occupational Health
Fidelis Jacklyn Adella, Moul Vanna, Bipin Adhikari, Sam Ol, Rupam Tripura, Chan Davoeung, James J. Callery, Yok Sovann, Arjun Chandna, Voeunrung Bunreth, Carina Asnong, Lorenz von Seidlein, Arjen M. Dondorp, Richard J. Maude, Yoel Lubell, Bridget Wills, Dysoley Lek, Thomas J. Peto
Summary: This study aimed to assess the feasibility of using point-of-care tests to diagnose acute febrile illnesses in primary care settings. The results showed that all three point-of-care tests performed well under assessment, but sample collection for the dengue test was difficult. Health workers found the diagnostics useful but not as convenient as standard malaria rapid tests.
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2023)
Review
Microbiology
Arkasha Sadhewa, Sarah Cassidy-Seyoum, Sanjaya Acharya, Angela Devine, Ric N. Price, Muthoni Mwaura, Kamala Thriemer, Benedikt Ley
Summary: Plasmodium vivax malaria continues to be a significant burden in various regions. The use of 8-aminoquinoline drugs is necessary for the complete removal of the parasite, but it can cause severe haemolysis in G6PD deficient patients. The implementation of routine G6PD testing in malaria endemic countries is still lacking, and there are challenges in training healthcare staff, quality control of diagnostics, and communication with affected communities.
Article
Health Care Sciences & Services
Kamala Thriemer, Robert James Commons, Megha Rajasekhar, Tamiru Shibiru Degaga, Krisin Chand, Nguyen Hoang Chau, Ashenafi Assefa, Mohammad Nader Naddim, Ayodhia Pitaloka Pasaribu, Awab Ghulam Rahim, Inge Sutanto, Tran Tinh Hien, Asrat Hailu, Mohammad Anwar Hasanzai, Lenny L. Ekawati, Adugna Woyessa, Tedla Teferi, Naomi Waithira, Walter R. J. Taylor, Benedikt Ley, Arjen Dondorp, J. Kevin Baird, Nicholas J. White, Nicholas P. Day, Ric N. Price, Julie A. Simpson, Lorenz von Seidlein
Summary: This study explored the differences in symptom reporting across multiple trial sites of an antimalarial trial and found significant variations in symptom reporting between different countries. The differences were greater for mild and moderate symptoms compared to severe symptoms.
BMC MEDICAL RESEARCH METHODOLOGY
(2023)
Review
Infectious Diseases
Parinaz Mehdipour, Megha Rajasekhar, Saber Dini, Sophie Zaloumis, Tesfay Abreha, Ishag Adam, Ghulam Rahim Awab, J. Kevin Baird, Larissa W. Brasil, Cindy S. Chu, Liwang Cui, Andre Daher, Margarete Gomes, Lilia Gonzalez-Ceron, Jimee Hwang, Harin Karunajeewa, Marcus V. G. Lacerda, Simone Ladeia-Andrade, Toby Leslie, Benedikt Ley, Kartini Lidia, Alejandro Llanos-Cuentas, Rhea J. Longley, Wuelton Marcelo Monteiro, Dhelio B. Pereira, Komal Raj Rijal, Kavitha Saravu, Inge Sutanto, Walter R. J. Taylor, Pham Vinh Thanh, Kamala Thriemer, Jose Luiz F. Vieira, Nicholas J. White, Lina M. Zuluaga-Idarraga, Philippe J. Guerin, Ric N. Price, Julie A. Simpson, Robert J. Commons, WWARN Vivax Adherence Study Grp
Summary: Reduced adherence increases the risk of P. vivax recurrence.
Editorial Material
Microbiology
Sarah Auburn
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Infectious Diseases
C. R. Walker, R. I. Hickson, E. Chang, P. Ngor, S. Sovannaroth, J. A. Simpson, D. J. Price, J. M. McCaw, R. N. Price, J. A. Flegg, A. Devine
Summary: Plasmodium falciparum and P. vivax are the two most common causes of malaria, with P. vivax posing a greater challenge to eliminating malaria due to its ability to cause relapsing episodes. Mixed infections of both species can complicate treatment and detection. A stochastic mathematical model was used to assess different treatment strategies, and it was found that a unified radical cure strategy and mass drug administration with schizonticidal treatment were effective in reducing malaria cases and deaths.
Article
Infectious Diseases
Wondimagegn Adissu, Marcelo Brito, Eduardo Garbin, Marcela Macedo, Wuelton Monteiro, Sandip Kumar Mukherjee, Jane Myburg, Mohammad Shafiul Alam, Germana Bancone, Pooja Bansil, Sampa Pal, Abhijit Sharma, Stephanie Zobrist, Andrew Bryan, Cindy S. Chu, Santasabuj Das, Gonzalo J. Domingo, Amanda Hann, James Kublin, Marcus V. G. Lacerda, Mark Layton, Benedikt Ley, Sean C. Murphy, Francois Nosten, Dhelio Pereira, Ric N. Price, Arunansu Talukdar, Daniel Yilma, Emily Gerth-Guyette
Summary: The study pooled data from clinical studies conducted in different countries to evaluate the performance of the STANDARD G6PD Test. The test showed high sensitivity and specificity in capillary and venous specimens, making it a reliable tool to guide the treatment of antimalarial drugs.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Infectious Diseases
Walter R. J. Taylor, Niamh Meagher, Benedikt Ley, Kamala Thriemer, Germana Bancone, Ari Satyagraha, Ashenafi Assefa, Krisin Chand, Nguyen Hoang Chau, Mehul Dhorda, Tamiru S. Degaga, Lenny L. Ekawati, Asrat Hailu, Mohammad Anwar Hasanzai, Mohammad Nader Naddim, Ayodhia Pitaloka Pasaribu, Awab Ghulam Rahim, Inge Sutanto, Ngo Viet Thanh, Nguyen Thi Tuyet-Trinh, Naomi Waithira, Adugna Woyessa, Arjen Dondorp, Lorenz von Seidlein, Julie A. Simpson, Nicholas J. White, J. Kevin Baird, Nicholas P. Day, Ric N. Price
Summary: This study focuses on the observational study of primaquine in patients with Plasmodium vivax malaria, and finds that a weekly dose of primaquine (PQ8W) is highly effective in preventing recurrence.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Hematology
Steven Kho, Nurjati C. Siregar, Labibah Qotrunnada, Aurelie Fricot, Abdoulaye Sissoko, Putu A. I. Shanti, Freis Candrawati, Noy N. Kambuaya, Hasrini Rini, Benediktus Andries, David Hardy, Nur I. Margyaningsih, Fauziyah Fadllan, Desandra A. Rahmayenti, Agatha M. Puspitasari, Leo Leonardo, Bagus T. G. Yayang, Dewi S. Margayani, Pak Prayoga, Leily Trianty, Enny Kenangalem, Ric N. Price, Tsin W. Yeo, Gabriela Minigo, Rintis Noviyanti, Jeanne R. Poespoprodjo, Nicholas M. Anstey, Pierre A. Buffet
Summary: Splenomegaly in malaria patients is associated with retention of uninfected red blood cells in the spleen, which is the major mechanism of anemia.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Review
Public, Environmental & Occupational Health
Holger W. Unger, Sanjaya Acharya, Lachlan Arnold, Connie Wu, Anna Maria van Eijk, Georgia R. Gore-Langton, Feiko O. ter Kuile, Elvin Lufele, R. Matthew Chico, Ric N. Price, Brioni R. Moore, Kamala Thriemer, Stephen J. Rogerson
Summary: Half of all pregnancies at risk of malaria occur in the Asia-Pacific region, where the co-existence of Plasmodium falciparum and Plasmodium vivax is common. Malaria control in pregnancy faces challenges due to high-grade antimalarial resistance and the need for more sensitive diagnostic tests. A more systematic approach is needed to prevent malaria in pregnancy in the Asia-Pacific region.
LANCET GLOBAL HEALTH
(2023)