Article
Cell Biology
Edwin C. Fluck, Aysenur Torun Yazici, Tibor Rohacs, Vera Y. Moiseenkova-Bell
Summary: Transient receptor potential vanilloid 5 (TRPV5) is a calcium-selective ion channel that plays a crucial role in maintaining calcium homeostasis in the kidney. This study investigates the regulation of TRPV5 by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) and calmodulin (CaM), as well as the effects of parathyroid hormone (PTH), metabolic acidosis, and protein kinase A (PKA) on TRPV5 activity. The findings reveal that low pH inhibits TRPV5 by preventing PI(4,5)P-2 activation, and PKA controls TRPV5 activity by preventing CaM binding and channel inactivation.
Article
Critical Care Medicine
Wang Deng, Di Qi, Xu-Mao Tang, Xin-Yu Deng, Jing He, Dao-Xin Wang
Summary: The study demonstrated that the WNK4/SPAK signaling pathway improved alveolar fluid clearance in LPS-induced ARDS by upregulating ENaC and reducing pulmonary edema, resulting in enhanced survival rate.
Article
Biochemistry & Molecular Biology
Yue Shi, Kaixuan Zhao, Guang Yang, Jia Yu, Yuxin Li, Michael M. Kessels, Lina Yu, Britta Qualmann, Per-Olof Berggren, Shao-Nian Yang
Summary: Endocytosis is regulated by a complex molecular machinery, in which syndapin I/PACSIN 1 plays a crucial role in pancreatic beta cells by interacting with neural Wiskott-Aldrich syndrome protein (N-WASP) to drive endocytosis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Moshe Katz, Suraj Subramaniam, Orna Chomsky-Hecht, Vladimir Tsemakhovich, Veit Flockerzi, Enno Klussmann, Joel A. Hirsch, Sharon Weiss, Nathan Dascal
Summary: CaV1.2 channels play a crucial role in regulating cardiac muscle contraction, with 0-AR increasing contraction by enhancing calcium influx through these channels via PKA activation. A recent study identified Rad as essential for PKA regulation of CaV1.2, revealing two distinct pathways of PKA modulation of CaV1.2: Rad dependent and Rad independent.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Wiebke Nadolni, Roland Immler, Kilian Hoelting, Marco Fraticelli, Myriam Ripphahn, Simone Rothmiller, Masayuki Matsushita, Ingrid Boekhoff, Thomas Gudermann, Markus Sperandio, Susanna Zierler
Summary: The study demonstrates the functional expression of TRPM7 in neutrophils and its impact on neutrophil recruitment during inflammation. Blocking TRPM7 channel or kinase activity affects neutrophil function, indicating a potential target to treat excessive neutrophil invasion.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Yoshiaki Suzuki, Tomo Kurata, Tsukasa Koide, Itsuki Okada, Nanami Nakajima, Yuji Imaizumi, Hisao Yamamura
Summary: This study reveals the existence of a complex located within caveolae in vascular smooth muscle cells that can convert changes in intracellular calcium concentrations to gene transcription profiles related to chemotaxis. CaMK1a is believed to be the key molecular identity that can transport calcium signals from caveolae to the nucleus.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Multidisciplinary Sciences
Premchand Rajeev, Nivedita Singh, Adel Kechkar, Cory Butler, Narendrakumar Ramanan, Jean-Baptiste Sibarita, Mini Jose, Deepak Nair
Summary: Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. The molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Additionally, the nanoscale molecular signatures of these signaling islands and phase transitions are altered in response to changes in cytosolic Ca2+, showing differential sensitivities to Ca2+ bound Calmodulin. SAP97/hDLG forms nanosized molecular domains in both heterologous cells and hippocampal pyramidal neurons, with molecular signatures and exchange kinetics being modulated by the availability of alternatively spliced isoforms.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Ying Li, Yan Feng, Li Liu, Xue Li, Xin-yu Li, Xun Sun, Ke-xin Li, Rong-rong Zha, Hong-dan Wang, Meng-di Zhang, Xiong-xiong Fan, Di Wu, Yao Fan, Hao-cheng Zhang, Guo-fen Qiao, Bai-yan Li
Summary: The study showed that H2S improves the autonomic control of blood pressure regulation by increasing K-ATP channel expression in the baroreflex afferent pathway, suggesting a crucial role of H2S in cardiovascular disorders.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Biochemistry & Molecular Biology
Justin M. Watkins, Timothy J. Ross-Elliott, Xiaoyi Shan, Fei Lou, Bernd Dreyer, Meral Tunc-Ozdemir, Haiyan Jia, Jing Yang, Celio Cabral Oliveira, Luguang Wu, Yuri Trusov, Timothy D. Schwochert, Patrick Krysan, Alan M. Jones
Summary: In Arabidopsis thaliana, GPCRs are not necessary for G protein-coupled signaling, as the seven-transmembrane protein AtRGS1 modulates G protein signaling through ligand-dependent endocytosis, with the origin of endocytosis depending on ligand identity. Different trafficking origins and trajectories result in different cellular outcomes in this system.
Article
Cell Biology
Samu Luostarinen, Mari Hämäläinen, Antti Pemmari, Eeva Moilanen
Summary: This study investigated the effects of Th1 and Th2-type inflammation on TRPA1 expression and function. The results showed that TRPA1 expression and function were enhanced under inflammatory conditions, with IFN-γ increasing TRPA1 expression and IL-4 and IL-13 suppressing it in a JAK-STAT6 dependent manner. These findings suggest that the paradigm of Th1 and Th2 inflammation is a major determinant of TRPA1 expression and function in (lung) disease and should be considered in pharmacotherapy.
INFLAMMATION RESEARCH
(2023)
Article
Physiology
Na Li, Ruixiu Shi, Yang Ye, Yingying Zhang, Yumeng Zhang, Zhizhi Wang, Ying Gu, Yongxiang Yin, Daozhen Chen, Jiaqi Tang
Summary: The incidence of cerebrovascular diseases increases significantly with aging. This study found that aging may influence the PKA-dependent vasodilation via the RyR/BKCa pathway in the middle cerebral arteries. In aged rats, the PKA/BKCa pathway was down-regulated, leading to decreased relaxation of the arteries and reduced BKCa channel activity. These findings provide new insights into the age-related cerebral vascular dysfunction and cerebrovascular diseases.
PHYSIOLOGICAL RESEARCH
(2022)
Article
Physiology
Daniela Rotin, Olivier Staub
Summary: The Epithelial Na+ Channel (ENaC) plays a critical role in regulating salt and fluid homeostasis in the body, controlling fluid reabsorption in the kidney, lung, and colon. Major advances over the past 25 years have been made in understanding ENaC structure, function, regulation, and its role in human diseases, including mutations associated with hypertension, hypotension, and other disorders.
COMPREHENSIVE PHYSIOLOGY
(2021)
Review
Physiology
David Pearce, Anna D. Manis, Viatcheslav Nesterov, Christoph Korbmacher
Summary: Regulated Na+ transport in the distal nephron is crucial for fluid and electrolyte homeostasis, controlling the levels and concentrations of various ions. Recent research has provided a clearer understanding of the transporters involved in moving Na+ in the distal nephron and their relationships with other ions, as well as the roles of signaling systems and components. Emerging evidence suggests that local K+ concentrations are involved in the reciprocal regulation of NCC and ENaC activity, adjusting renal K+ secretion in response to dietary intake.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Physiology
Sasanka Ramanadham, John Turk, Sushant Bhatnagar
Summary: Impaired glucose tolerance and β-cell dysfunction in insulin resistance associated with obesity leads to the development of type 2 diabetes. Recent research suggests that both canonical and novel noncanonical pathways play a role in β-cell (dys)function. Therefore, targeting both pathways could be a more comprehensive approach for restoring β-cell function in the context of impaired glucose tolerance associated with obesity and type 2 diabetes.
COMPREHENSIVE PHYSIOLOGY
(2023)