Article
Biotechnology & Applied Microbiology
Ya-Ping Wang, Lin Liu, Xue-Shan Wang, Kun-Qiang Hong, Li-Hua Zhang, Zhong-Guan Sun, Dong-Guang Xiao
Summary: The study shows that the GAT1 gene plays a crucial role in regulating higher alcohol production in Saccharomyces cerevisiae, with its deletion leading to decreased levels of higher alcohols. The downregulation of specific genes is partly responsible for this effect. These findings provide important insights for the wheat beer industry, offering valuable guidance for production and future research.
BIOENGINEERING-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Aroa Ferrer-Pinos, Victor Garrigos, Emilia Matallana, Agustin Aranda
Summary: Wine strains of Saccharomyces cerevisiae need to adapt their metabolism to different growth conditions during biotechnological applications, with the transcription factor Gln3 playing a crucial role in glucose and nitrogen repression. Commercial yeast strains exhibit a variety of glucose repression profiles, and deletion of the GLN3 gene results in different mutant phenotypes. The nitrogen catabolite repression controlled by TORC1 is largely conserved in winemaking conditions, with mutants showing increased tolerance to rapamycin inhibition of nutrient signaling complex TORC1.
FERMENTATION-BASEL
(2021)
Article
Multidisciplinary Sciences
Piotr Hapeta, Patrycja Szczepanska, Cecile Neuveglise, Zbigniew Lazar
Summary: YlHxk1's loop region is essential for its activity and growth of Y. lipolytica on glucose and fructose. The loop hinders binding with T6P and affects lipase-encoding genes expression, as well as the preference for glycerol over glucose.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Marie Sarah Evers, Louise Ramousse, Christophe Morge, Celine Sparrow, Antoine Gobert, Chloe Roullier-Gall, Herve Alexandre
Summary: This study investigates the vitamin requirements of S. cerevisiae during winemaking by monitoring the evolution of 8 water-soluble vitamins and their different vitamers during fermentation. It identifies that B1 is required for fermentation, B8 is required for growth, and B5 is required for both processes. The study quantifies the extent of B5 requirement, determining that 750 & mu;g.L-1 is sufficient to cover the yeast's needs.
Article
Microbiology
Abhinav Nair, Saurabh Jyoti Sarma
Summary: Organisms use catabolite repression to adjust resource utilization to gain a competitive edge in unfavorable conditions, playing a crucial role in microbial survival. Studying genes and proteins linked to preferential uptake mechanisms can identify regulatory processes that promote microbial growth and survival.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Genetics & Heredity
Jordan B. Barney, Dakshayini G. Chandrashekarappa, Samantha R. Soncini, Martin C. Schmidt
Summary: This study reveals that diploid yeast cells acquire resistance to the glucose analog, 2-deoxyglucose, through dominant alleles, haploinsufficiency, gene duplication, and aneuploidy, while haploid cells primarily use recessive alleles. The methods of resistance include missense alleles in AMP-activated protein kinase subunits, nonsense alleles in the REG1 gene, and increased expression of hexose transporters. Aneuploidy, although imposing a fitness cost, offers potential reversibility of drug resistance.
Review
Biotechnology & Applied Microbiology
Joshua D. D. Kerkaert, Lori B. B. Huberman
Summary: Organisms need to accurately sense and respond to nutrients for survival. Fungi have evolved genetic networks to regulate metabolic pathways and distinguish between preferred and nonpreferred nutrients. Transcription factors and regulatory genes play important roles in this regulation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Isabelle Georis, Aria Ronsmans, Fabienne Vierendeels, Evelyne Dubois
Summary: Nitrogen catabolite repression (NCR) is a mechanism in yeast that regulates its gene expression in response to changing nitrogen sources. This study examines the role of the SAGA complex in the diverse NCR responses observed.
Article
Multidisciplinary Sciences
Thoma Itoh, Takashi Makino
Summary: Recent advancements in high throughput single cell RNA-seq have enabled the identification of functional compensation effects of gene perturbations through noise change, which can be measured quantitatively and captured in gene regulatory networks.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Xiaowei Gong, Huajun Luo, Liu Hong, Jun Wu, Heng Wu, Chunxia Song, Wei Zhao, Yi Han, Ya Dao, Xia Zhang, Donglai Zhu, Yiyong Luo
Summary: The study found that tryptophan significantly promoted the production of tryptophol (TOL), but the output plateaued at a certain concentration, while phenylalanine reduced the stimulating effect of tryptophan. The effects of these two amino acids on TOL production were related to the transamination and decarboxylation of the Ehrlich pathway.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xin-Xin Kang, Qin-Qing Wang, Zhe Chi, Guang-Lei Liu, Zhong Hu, Zhen-Ming Chi
Summary: The study showed that by removing GATA-type transcriptional activator and repressor genes, as well as overexpressing mutated genes with specific residues, the pullulan production of Aureobasidium melanogenum P16 could be significantly increased.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Lana O'Hara Souza Silva, Thalison Rodrigues Moreira, Relber Aguiar Goncales, Mariana Vieira Tomazett, Juliana Alves Parente-Rocha, Karine Mattos, Juliano Domiraci Paccez, Orville Hernandez Ruiz, Maristela Pereira, Celia Maria de Almeida Soares, Simone Schneider Weber, Vanessa Rafaela Milhomem Cruz-Leite, Clayton Luiz Borges
Summary: Nitrogen is crucial for microorganisms, but hosts limit this nutrient to counter infections, thereby pathogens use adaptive mechanisms. In fungi, nitrogen catabolite repression (NCR) activates transcription factors to acquire nitrogen from alternative sources. This study investigates the importance of formamidase in Paracoccidioides lutzii and finds that formamidase plays a role in nitrogen assimilation and fungal virulence.
Article
Biotechnology & Applied Microbiology
Huili Xia, Lingling Shangguan, Sheng Chen, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Jun Dai, Xiong Chen
Summary: This study focuses on the biosynthesis of 2-phenylethanol (2-PE) using whole-cell bioconversion with Saccharomyces cerevisiae. It is found that the addition of rapamycin (Rap) can enhance the efficiency of 2-PE synthesis by increasing the flux of the Ehrlich pathway. The key role of GLN3 in the regulation of Rap is also identified. Commercial yeast is verified to be applicable for 2-PE production. The results demonstrate a new approach for the efficient synthesis of 2-PE.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Hongting Tang, Lianghuan Wu, Shuyuan Guo, Wenbing Cao, Wenhui Ma, Xiang Wang, Junfeng Shen, Menglin Wang, Qiannan Zhang, Mingtao Huang, Xiaozhou Luo, Jie Zeng, Jay D. Keasling, Tao Yu
Summary: The need for more efficient and sustainable production strategies for foods and chemicals has been emphasized due to population-related and environmental issues. A hybrid system that combines the electrochemical reduction of CO2 with the transformation of intermediates using engineered microorganisms has been identified. This system enables the production of valuable products from C1-3 substrates. By rewiring metabolic processes and mitigating glucose repression, high concentrations of glucose and sucrose were achieved. These findings provide guidance for the production of microbial sugar-derived foods and chemicals from renewable reduced CO2-based feedstocks.
Article
Biotechnology & Applied Microbiology
Qin Yan, Laichuang Han, Xinyue Liu, Cuiping You, Shengmin Zhou, Zhemin Zhou
Summary: An efficient auto-inducible protein expression system based on nitrogen catabolite regulation was constructed in this study. The system successfully expressed glycosylated and secretory proteins, providing a new option for protein production in laboratory and industrial applications.
MICROBIAL CELL FACTORIES
(2022)
Article
Genetics & Heredity
Rajendra Rai, Jennifer J. Tate, Karthik Shanmuganatham, Martha M. Howe, David Nelson, Terrance G. Cooper
Article
Biochemistry & Molecular Biology
Georis Isabelle, Jennifer J. Tate, Fabienne Vierendeels, Terrance G. Cooper, Evelyne Dubois
Article
Genetics & Heredity
Jennifer J. Tate, Isabelle Georis, Rajendra Rai, Fabienne Vierendeels, Evelyne Dubois, Terrance G. Cooper
G3-GENES GENOMES GENETICS
(2015)
Article
Genetics & Heredity
Jennifer J. Tate, David Buford, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Rajendra Rai, Jennifer J. Tate, Terrance G. Cooper
G3-GENES GENOMES GENETICS
(2016)
Article
Genetics & Heredity
Jennifer J. Tate, David Buford, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Jennifer J. Tate, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Jennifer J. Tate, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Jennifer J. Tate, Rajendra Rai, Terrance G. Cooper
Article
Biochemistry & Molecular Biology
Andre Feller, Isabelle Georis, Jennifer J. Tate, Terrance G. Cooper, Evelyne Dubois
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Rajendra Rai, Jennifer J. Tate, David R. Nelson, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Rajendra Rai, Jennifer J. Tate, Isabelle Georis, Evelyne Dubois, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Jennifer J. Tate, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Rajendra Rai, Jennifer J. Tate, Karthik Shanmuganatham, Martha M. Howe, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Microbiology
Mohammad Fayyadkazan, Jennifer J. Tate, Fabienne Vierendeels, Terrance G. Cooper, Evelyne Dubois, Isabelle Georis