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Medicine, Research & Experimental
Bo Qian, Lin Che, Ze-Bang Du, Ni-jun Guo, Xin-Mou Wu, Lei Yang, Zhao-Xuan Zheng, Yun-Lu Gao, Ming-Zhu Wang, Xiao-Xuan Chen, Ling Xu, Zi-Jian Zhou, Yu-Chun Lin, Zhong-Ning Lin
Summary: The phosphorylation/dephosphorylation modification of GPX4 affects ferroptosis in HCC cells, with PP2A-B55 beta playing a role in GPX4 dephosphorylation. The PP2A-B55 beta/p-GPX4(Ser2)/p53 axis is a novel regulatory pathway of Sora-induced ferroptosis. These findings have potential implications for improving the efficacy of Sora in HCC treatment.
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Biochemistry & Molecular Biology
Xiaowei Nie, Chenyou Shen, Jianxin Tan, Xusheng Yang, Wei Wang, Youai Dai, Haijian Sun, Zhiyuan Wu, Jingyu Chen
Summary: The study demonstrated that Andrographolide (ANDRO) may reverse pulmonary vascular remodeling through modulation of NOX/Nrf2-mediated oxidative stress and NF-kappa B-mediated inflammation, suggesting therapeutic potential in the treatment of pulmonary hypertension.
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Multidisciplinary Sciences
Takahiro Masaki, Makoto Habara, Shunsuke Hanaki, Yuki Sato, Haruki Tomiyasu, Yosei Miki, Midori Shimada
Summary: c-Myc, a transcription factor, is often highly expressed in cancers. This study found that the intracellular Ca2+ concentration regulates c-Myc stability. Calcineurin, a Ca2+-dependent protein phosphatase, was identified as a positive regulator of c-Myc expression. Inhibition of calcineurin decreased c-Myc expression and enhanced phosphorylation in a mouse xenograft model. Therefore, calcineurin could be a new therapeutic target for c-Myc-overexpressing cancers.
SCIENTIFIC REPORTS
(2023)
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Biochemistry & Molecular Biology
Fiona Jenkinson, Kang Wei Tan, Barbara Schopf, Miguel M. Santos, Philip Zegerman
Summary: In eukaryotes, cyclin-dependent kinase (CDK) inhibits helicase loading factors to ensure the exact duplication of the genome. CDK activates origin firing by phosphorylating Sld2 and Sld3, forming a transient intermediate called the pre-initiation complex (pre-IC). In yeast, the CDK phosphorylations of Sld3 and Sld2 are rapidly turned over by the phosphatases PP2A and PP4 during S phase. This dephosphorylation is important for genome-wide origin firing, pre-IC formation, and maintaining Sld3 dephosphorylation in G1 phase. PP2ARts1 specifically targets Sld3 and its dephosphorylation is critical for replication and cell viability.
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Multidisciplinary Sciences
Jingchun Du, Yougui Xiang, Hua Liu, Shuzhen Liu, Ashwani Kumar, Chao Xing, Zhigao Wang
Summary: PPP1R3G regulates cell death by recruiting PP1 gamma to dephosphorylate inhibitory sites on RIPK1, playing a crucial role in apoptosis and necroptosis as well as inflammation regulation.
NATURE COMMUNICATIONS
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Oncology
Shunya Tsuji, Naoki Kohyanagi, Takuya Mizuno, Takashi Ohama, Koichi Sato
Summary: The study demonstrated that the anti-psychotic drug perphenazine exerted antitumor effects by reactivating PP2A in the neuroblastoma rat sarcoma oncogene (NRAS)-mutated Sezary syndrome cell line HUT78, inducing dephosphorylation of Akt and ERK1/2 and triggering apoptosis. Inhibition of Akt activity through PP2A activation may be an attractive antitumor strategy for NRAS-mutated Sezary syndrome.
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Biochemistry & Molecular Biology
Hyuk Sung Yoon, Kaien Fujino, Shenkui Liu, Tetsuo Takano, Daisuke Tsugama
Summary: The study shows that Arabidopsis PP2A B''-family subunits interact with PP2A A (scaffold) subunits and can mediate VIP1 dephosphorylation. This provides important insights into the molecular mechanism of VIP1 dephosphorylation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
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Clinical Neurology
Maria E. Prados, Alejandro Correa-Saez, Juan D. Unciti-Broceta, Martin Garrido-Rodriguez, Carla Jimenez-Jimenez, Massimiliano Mazzone, Alberto Minassi, Giovanni Appendino, Marco A. Calzado, Eduardo Munoz
Summary: The compound BAH modifies PHD2 activity to stabilize HIF-1α, induces gene expression, and protects against cytotoxicity in cells with mutated huntingtin protein. Pharmacokinetic analyses show BAH's ability to improve symptoms in a mouse model of striatal neurodegeneration and impact key pathways for the treatment of HD and other neurodegenerative diseases.
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Cardiac & Cardiovascular Systems
Min Ji Cho, Dong Gwang Lee, Jeong Woong Lee, Byungtae Hwang, Sung-Jin Yoon, Seon-Jin Lee, Young-Jun Park, Seung-Ho Park, Hee Gu Lee, Yong-Hoon Kim, Chul-Ho Lee, Jangwook Lee, Nam-Kyung Lee, Tae-Su Han, Hyun-Soo Cho, Jeong Hee Moon, Ga Seul Lee, Kwang-Hee Bae, Geum-Sook Hwang, Sang-Hak Lee, Sang J. Chung, Sungbo Shim, Jaehyung Cho, Goo Taeg Oh, Young-Guen Kwon, Jong-Gil Park, Jeong-Ki Min
Summary: This study investigates the role of protein tyrosine phosphatase type IVA1 (PTP4A1) in the negative regulation of the NF-kappa B signalling pathway in endothelial cells (ECs). The findings suggest that PTP4A1 regulates NF-kappa B inactivation through the USF1/A20 axis, and its expression or activation in ECs may be useful for the treatment of vascular inflammatory diseases.
CARDIOVASCULAR RESEARCH
(2023)
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Biochemistry & Molecular Biology
Yaoyao Xue, Chunshuang Li, Shihua Deng, Xin Chen, Jinling Han, Xu Zheng, Miaomiao Tian, Wenjing Hao, Lang Pan, Istvan Boldogh, Xueqing Ba, Ruoxi Wang
Summary: Reactive oxygen species (ROS) regulate the activity of nuclear factor kappa B (NF-KB) by altering the phosphorylation of RelA/p65 at Ser276. The repair enzyme OGG1 binds to 8-oxoGua, facilitating the binding of NF-KB to promoters and enhancing the transcription of pro-inflammatory cytokines and chemokines. Scavenging ROS or inhibiting OGG1 decreases the phosphorylation of Ser276, leading to reduced expression of ROS-responsive genes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
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Oncology
Isha Mondal, Oishika Das, Raymond Sun, Jian Gao, Bohyeon Yu, Aaron Diaz, Jinan Behnan, Abhishek Dubey, Zhipeng Meng, Emad Eskandar, Beisi Xu, Rongze Olivia Lu, Winson S. Ho
Summary: This study demonstrates the important role of the a-isoform of the catalytic subunit of protein phosphatase-2A (PP2Ac) in regulating the immunogenicity of glioma. The deficiency of PP2Ac in glioma cells promotes dendritic cell (DC) cross-presentation, sensitizes tumors to immune-checkpoint blockade and radiotherapy treatment. Loss of PP2Ac increases interferon signaling and reduces the expression of a tumor gene signature associated with worse patient survival.
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Multidisciplinary Sciences
Maria N. Muniz Garcia, Cecilia Grossi, Rita M. Ulloa, Daniela A. Capiati
Summary: The serine/threonine protein phosphatases type 2A (PP2A) play important roles in developmental programs, stress responses, and hormone signaling in plants. This study focused on the StPP2Ac2b subunit in cultivated potatoes and found that its overexpression leads to increased susceptibility to the pathogen Phytophthora infestans, promoting senescence.
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Medicine, Research & Experimental
Shibin Feng, Hongyan Ding, Leihong Liu, Chenglu Peng, Yingying Huang, Fuchao Zhong, Wei Li, Tingting Meng, Jinchun Li, Xichun Wang, Yu Li, Jinjie Wu
Summary: The study demonstrated that the immunoregulatory effects of Astragalus polysaccharide on RAW264.7 cells are mediated, at least in part, via the activation of the NF-kappa B p65/MAPK signaling pathway.
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(2021)
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Biochemistry & Molecular Biology
Hansol Lee, Yun-Jin Hwang, Jung-Hyun Park, Du-Hyong Cho
Summary: This study investigates the effect of valproic acid (VPA) on vascular smooth muscle cell (VSMC) proliferation and its mechanism. The results show that VPA decreases cell proliferation through PP2A-mediated inhibition of p-p70S6K-Thr(389). This suggests that VPA could be used in the treatment and prevention of atherosclerosis and in-stent restenosis.
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(2022)
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Cardiac & Cardiovascular Systems
Julius R. Herting, Jule H. Koenig, Katarina Hadova, Alexander Heinick, Frank U. Mueller, Paul Pauls, Matthias D. Seidl, Carolina Soppa, Uwe Kirchhefer
Summary: PR72 overexpression in myocardial cells results in mild cardiac hypertrophy and increased contractility. This is accompanied by an increase in the peak amplitude of Ca2+ transients, a shorter decay in intracellular Ca2+ levels, and longer duration and wider width of Ca2+ sparks. Consistent with the contractile data, phosphorylation of phospholamban is also increased in TG hearts, while expression of the Na+/Ca2+ exchanger is decreased.
FRONTIERS IN CARDIOVASCULAR MEDICINE
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Po-Sheng Yang, Meng-Jiy Wang, Thanasekaran Jayakumar, Duen-Suey Chou, Ching-Ya Ko, Ming-Jen Hsu, Cheng-Ying Hsieh
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Yu-Fan Chuang, Mei-Chieh Chen, Shiu-Wen Huang, Ya-Fen Hsu, George Ou, Yu-Jou Tsai, Ming-Jen Hsu
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Multidisciplinary Sciences
Yu-Han Huang, Shiu-Wen Huang, Ya-Fen Hsu, George Ou, Wei-Jan Huang, Ming-Jen Hsu
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Ruo-Kai Lin, Yuh-Feng Lin, Ming-Jen Hsu, Chang-Lin Hsieh, Chen-Yu Wang, Chih-Chiang Huang, Wei-Jan Huang
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2016)
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Ting-Lin Yen, Ray-Jade Chen, Thanasekaran Jayakumar, Wan-Jung Lu, Cheng-Ying Hsieh, Ming-Jen Hsu, Chih-Hao Yang, Chao-Chien Chang, Yen-Kuang Lin, Kuan-Hung Lin, Joen-Rong Sheu
TRANSLATIONAL RESEARCH
(2016)
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Multidisciplinary Sciences
Chia-Sheng Yen, Jung-Chien Chen, Yi-Fang Chang, Ya-Fen Hsu, Pei-Ting Chiu, Ching Shiue, Yu-Fan Chuang, George Ou, Ming-Jen Hsu
SCIENTIFIC REPORTS
(2016)
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Biochemistry & Molecular Biology
Yi Chang, Jiun-Yi Li, Thanasekaran Jayakumar, Shou-Huang Hung, Wei-Cheng Lee, Manjunath Manubolu, Joen-Rong Sheu, Ming-Jen Hsu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
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Joen-Rong Sheu, Zhih-Cherng Chen, Ming-Jen Hsu, Shwu-Huey Wang, Kuo-Wei Jung, Wei-Fan Wu, Szu-Han Pan, Ruei-Dun Teng, Chih-Hao Yang, Cheng-Ying Hsieh
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2018)
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Pharmacology & Pharmacy
Chia-Sheng Yen, Cheuk-Sing Choy, Wei-Jan Huang, Shiu-Wen Huang, Pin-Ye Lai, Meng-Chieh Yu, Ching Shiue, Ya-Fen Hsu, Ming-Jen Hsu
FRONTIERS IN PHARMACOLOGY
(2018)
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Yu-Han Huang, Hung-Yu Yang, Shiu-Wen Huang, George Ou, Ya-Fen Hsu, Ming-Jen Hsu
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Yi-Fang Chang, Ya-Fen Hsu, Pei-Ting Chiu, Wei-Jan Huang, Shiu-Wen Huang, George Ou, Joen-Rong Sheu, Ming-Jen Hsu
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Jin-Cherng Lien, Chi-Li Chung, Tur-Fu Huang, Tsung-Chia Chang, Kuan-Chung Chen, Ging-Yan Gao, Ming-Jen Hsu, Shiu-Wen Huang
BRITISH JOURNAL OF PHARMACOLOGY
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Shiu-Wen Huang, I-Tsu Chyuan, Ching Shiue, Meng-Chieh Yu, Ya-Fen Hsu, Ming-Jen Hsu
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Yu-Fan Chuang, Shiu-Wen Huang, Ya-Fen Hsu, Meng-Chieh Yu, George Ou, Wei-Jan Huang, Ming-Jen Hsu
BRITISH JOURNAL OF PHARMACOLOGY
(2017)