Review
Biology
Cameron D. Haydinger, Liam M. Ashander, Alwin Chun Rong Tan, Justine R. Smith
Summary: Intercellular adhesion molecule 1 (ICAM-1) is a transmembrane protein that plays important roles in the immune system and is involved in various diseases. It mediates cellular adhesive interactions and is upregulated in inflammation. This review provides a comprehensive summary of the structure and regulation of ICAM1 gene and the ICAM-1 protein, discusses its functions in the immune system and diseases, and explores current therapeutics and future advancements.
Article
Multidisciplinary Sciences
Dicky L. Tahapary, Tasykuru Rizqa, Cut Vania Syarira, Lusiani Lusiani, Aulia Rizka, Syahidatul Wafa, Wismandari Wisnu, Tri Juli Edi Tarigan, Dante Saksono Harbuwono
Summary: This study aimed to determine the changes in ICAM-1 levels in patients with type 2 diabetes mellitus (T2DM) and non-DM patients during Ramadan fasting. The results showed that both T2DM and non-DM patients had a significant decrease in ICAM-1 levels after Ramadan fasting, but only non-DM patients had a significantly lower post-Ramadan ICAM-1 level.
Article
Biochemistry & Molecular Biology
Nina M. van Sorge, Daniel A. Bonsor, Liwen Deng, Erik Lindahl, Verena Schmitt, Mykola Lyndin, Alexej Schmidt, Olof R. Nilsson, Jaime Brizuela, Elena Boero, Eric J. Sundberg, Jos A. G. van Strijp, Kelly S. Doran, Bernhard B. Singer, Gunnar Lindahl, Alex J. McCarthy
Summary: Streptococcus agalactiae, also known as group B Streptococcus (GBS), is a major cause of neonatal sepsis in humans. In a recent study, it was found that the surface-expressed beta protein of GBS binds to human CEACAM1 and CEACAM5 receptors, with the crystal structure revealing a novel IgI3 fold. This discovery suggests a potentially widely applicable mechanism for bacteria to target CEACAMs.
Article
Oncology
Bing Zhu, Xinyan Wang, Takaya Shimura, Andrew C. Huang, Nana Kong, Yujie Dai, Jianmin Fang, Peng Guo, Jie-Er Ying
Summary: This preclinical study reports two effective targeted therapeutics for the treatment of cholangiocarcinoma. By conjugating ICAM1 antibodies with different cytotoxic payloads, tumor growth was successfully attenuated. This study paves the road for developing a promising targeted therapeutic candidate for clinical treatment of cholangiocarcinoma.
NPJ PRECISION ONCOLOGY
(2023)
Article
Cell Biology
Eun-Ji Lim, Jae-Hyeok Kang, Yeon-Ju Kim, Seungmo Kim, Su-Jae Lee
Summary: ICAM-1 is a potential therapeutic target for colorectal cancer (CRC) that enhances treatment effectiveness by promoting the c-MET-SRC signaling axis. Treatment with antibodies targeting ICAM-1 can reduce metastasis and angiogenesis in CRC.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Akira Sakamoto, Hiroyuki Inoue, Shohei Miyamoto, Shun Ito, Yasushi Soda, Kenzaburo Tani
Summary: Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related deaths globally. This study highlights the potential of oncolytic virotherapy using coxsackievirus A11 (CVA11) as a less toxic and more effective treatment for NSCLC. CVA11 infection showed extensive oncolytic activity in human NSCLC cell lines and induced immunogenic cell death. In vivo experiments also demonstrated complete tumor regression without significant weight loss. Further investigation in clinical trials, especially in combination with immunotherapy, is warranted.
SCIENTIFIC REPORTS
(2023)
Article
Ophthalmology
Natalia I. Khramenko, Natalia V. Konovalova, Volodymyr Ya. Usov, Liudmyla M. Velychko, Olexandra V. Bogdanova
Summary: The parameters of the T-cell link of the immune response and the parameters of humoral immunity were different between patients with uncomplicated recurrent anterior uveitis (AU) and those with recurrent AU complicated by uveitic macular edema (UME). The patients with UME had increased T-cell immune response parameters and decreased humoral immunity parameters. The relative numbers of natural killer cells and phagocytic neutrophils were also different between the two groups. The expression levels of ICAM-1 and CD5 seem to play a role in uveitic macular edema.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Oncology
Xiang Shu, Qin Zhou, Xiaohui Sun, Michelle Flesaker, Xingyi Guo, Jirong Long, Mark E. Robson, Xiao-Ou Shu, Wei Zheng, Jonine L. Bernstein
Summary: This study identified 98 proteins that may have potential causal associations with the risk of breast cancer subtypes. The associations of ICAM1, PLA2R1, and TXNDC12 varied significantly across the subtypes. These findings contribute to understanding the heterogeneity in the etiology of different subtypes of breast cancer and may aid in personalized risk assessment of the disease.
BRITISH JOURNAL OF CANCER
(2022)
Article
Immunology
Jae-Young Park, Hyo-Min Park, Seonhwa Kim, Kyeong-Bae Jeon, Chae-Min Lim, Jin Tae Hong, Do-Young Yoon
Summary: IL-32 theta A94V, a mutant isoform, can inhibit monocyte-endothelial adhesion by binding to integrins aVb3 and aVb6, thus reducing the early step of atherosclerosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jia Guo, Zeyu Xu, Rachel C. Gunderson, Baohui Xu, Sara A. Michie
Summary: In this study, the researchers found that ICAM-1 is expressed on the sinus endothelia of lymph nodes, but not on the lymphatic vessels of peripheral tissues. Memory CD4(+) T cells migrated to lymph nodes from peripheral tissues more efficiently than from blood vessels. ICAM-1 deficiency significantly inhibited the migration of lymphocytes from peripheral tissues into lymph nodes, and the absence of ICAM-1 or LFA-1 reduced the number of memory T cells in lymph nodes.
Article
Biotechnology & Applied Microbiology
Weiwei Cao, Yu Chen, Wei Han, Juzheng Yuan, Weimin Xie, Kun Liu, Yan Qiu, Xudan Wang, Xiao Li
Summary: This study found that patients with normal or normalized AFP and sICAM-1 levels in liver cancer had lower tumor recurrence rates and better survival rates, while those with elevated levels of both markers had poorer outcomes. AFP and ICAM-1 play crucial roles in the diagnosis, prognostic prediction, and immunotherapy of HCC, and their combined application can improve predictive accuracy.
Article
Microbiology
Lisa C. Hennemann, Shantelle L. LaFayette, Julien K. Malet, Perrine Bortolotti, Tianxiao Yang, Geoffrey A. McKay, Daniel Houle, Danuta Radzioch, Simon Rousseau, Dao Nguyen
Summary: Loss-of-function lasR mutants of Pseudomonas aeruginosa induce increased levels of airway epithelial ICAM-1, leading to enhanced neutrophilic inflammation and exacerbating lung disease progression. This study sheds light on the intricate interplay between lasR mutants, LasR-regulated proteases, and airway epithelial ICAM-1 expression, revealing a new mechanism involved in the exaggerated inflammatory response induced by lasR mutants.
Article
Biochemistry & Molecular Biology
Vladana Domazetovic, Irene Falsetti, Caterina Viglianisi, Kristian Vasa, Cinzia Aurilia, Maria Stio, Stefano Menichetti, Teresa Iantomasi
Summary: The study found that tocopherol derivatives can prevent TNFα-induced oxidative stress and inhibit the increase of ICAM-1 and Cl-2 expression. Among them, (δ-Toc)2S and (δ-Toc)2S-2 were more effective than δ-Toc and α-Toc.
Article
Oncology
Jing Zhang, Rongrong Zhang, Zhenhong Zhao
Summary: Clinical treatment can significantly reduce the levels of autoantibodies and ICAM-1 in patients with Graves disease, improve thyroid function, and alleviate inflammation. Detection of these indicators can provide guidance for disease progression and treatment.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
Article
Immunology
Mona Singh, Mony Thakur, Manish Mishra, Manisha Yadav, Rajkamal Vibhuti, Athira M. Menon, Girima Nagda, Ved Prakash Dwivedi, Tikam Chand Dakal, Vinod Yadav
Summary: ICAM-1 is a key inducible cell adhesion molecule involved in immune functions, with its expression regulated by various inflammatory mediators such as transcription factors and signaling pathways. Transcriptional regulation and epigenetic modifications play important roles in controlling ICAM-1 expression.
IMMUNOLOGY LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Stephen J. K. O'Neill, Zehuan Huang, Mohammed H. Ahmed, Alexander J. Boys, Santiago Velasco-Bosom, Jiaxuan Li, Roisin M. Owens, Jade A. McCune, George G. Malliaras, Oren A. Scherman
Summary: This study introduces an electrically conductive supramolecular polymer network that exhibits both electronic and ionic conductivity and maintains tissue-mimetic mechanical properties. By designing an ultrahigh affinity host-guest ternary complex and embedding it as dynamic cross-links, a conducting polymer with high stretchability and rapid self-recovery was synthesized.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ju An Park, Yejin Youm, Hwa-Rim Lee, Yongwoo Lee, Sarah L. Barron, Taejeong Kwak, Gyu Tae Park, Young-Cheol Song, Roisin M. Owens, Jae Ho Kim, Sungjune Jung
Summary: A cell sheet transfer method from a culture surface to a target tissue is introduced in this study. UV-treated parylene, a commercially available flexible material, is used as the culture surface to provide adequate cell adhesiveness for both cell attachment during culture and efficient transfer. Various cell types are successfully transferred using this versatile cell-transfer process. The mechanism of interfacial cell migration between surfaces with different adhesion preferences is observed, and the re-establishment and reversal of cell polarity after transfer are identified. The unique capabilities of 3D multilayer stacking, freeform design, and curved surface application are demonstrated. The therapeutic potential of the cell-sheet delivery system is demonstrated through experiments on cutaneous wound healing and skin-tissue regeneration in mice models.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Alexander J. Boys, Alejandro Carnicer-Lombarte, Amparo Gueemes-Gonzalez, Douglas C. van Niekerk, Sam Hilton, Damiano G. Barone, Christopher M. Proctor, Roisin M. Owens, George G. Malliaras
Summary: Bioelectronics is crucial for understanding and treating disease, but stable, long-term interfaces with the body are challenging. Combining techniques from regenerative medicine and electronics can create implants that integrate with tissue and enable sophisticated recording and stimulation. A hybrid implant system using a microelectrode array and a bioresorbable gel is constructed and shown to produce minimal foreign body response when implanted in muscle, allowing for long-term electromyographic signal recording with high resolution. This opens up possibilities for a new generation of long-term implantable electronics.
ADVANCED MATERIALS
(2023)
Article
Physics, Applied
Begona Abad, Kirstin Alberi, Katherine E. Ayers, Sushmee Badhulika, Chunmei Ban, Helene Bea, Fanny Beron, Julie Cairney, Jane P. Chang, Christine Charles, Mariadriana Creatore, Hui Dong, Jia Du, Renate Egan, Karin Everschor-Sitte, Cathy Foley, Anna Fontcuberta i Morral, Myung-Hwa Jung, Hyunjung Kim, Sarah Kurtz, Jieun Lee, Diana C. Leitao, Kristina Lemmer, Amy C. Marschilok, Bogdana Mitu, Bonna K. Newman, Roisin Owens, Anna-Maria Pappa, Youngah Park, Michelle Peckham, Liane M. Rossi, Sang-Hee Shim, Saima Afroz Siddiqui, Ji-Won Son, Sabina Spiga, Sedina Tsikata, Elisa Vianello, Karen Wilson, Hiromi Yuasa, Ilaria Zardo, Iryna Zenyuk, Yanfeng Zhang, Yudi Zhao
Summary: Women have made significant contributions to applied physics research and their involvement is crucial for ongoing progress. The Roadmap on Women in Applied Physics, written by female scientists and engineers, aims to celebrate their achievements, showcase established and early career researchers expanding boundaries in their fields, and increase visibility of women's impact on applied physics research. The topics covered include plasma materials processing and propulsion, super-resolution microscopy, bioelectronics, spintronics, superconducting quantum interference device technology, quantum materials, 2D materials, catalysis and surface science, fuel cells, batteries, photovoltaics, neuromorphic computing and devices, nanophotonics and nanophononics, and nanomagnetism. The intention is to inspire more women to enter these fields and foster inclusivity within the scientific community.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2023)
Article
Multidisciplinary Sciences
Georg Krainer, Kadi L. Saar, William E. Arter, Timothy J. Welsh, Magdalena A. Czekalska, Raphael P. B. Jacquat, Quentin Peter, Walther C. Traberg, Arvind Pujari, Akhila K. Jayaram, Pavankumar Challa, Christopher G. Taylor, Lize-Mari van der Linden, Titus Franzmann, Roisin M. Owens, Simon Alberti, David Klenerman, Tuomas P. J. Knowles
Summary: The authors developed a digital immunosensor assay called DigitISA, which uses microchip electrophoretic separation and single-molecule detection to quantitate protein biomarkers in a single, solution-phase step. This method overcomes the limitations of surface-capture based assays and provides more information beyond simple concentration measurements, making it important for protein biomarker detection.
NATURE COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Karan Bali, Charlotte Guffick, Reece McCoy, Zixuan Lu, Clemens F. Kaminski, Ioanna Mela, Roisin M. Owens, Hendrik W. van Veen
Summary: With the increasing threat of antibiotic resistance, there is a need to develop antimicrobials against highly resistant pathogenic bacteria. This study focuses on the ATP-binding cassette (ABC) transporter MsbA, which is crucial for the survival of Gram-negative pathogenic bacteria. Supported lipid bilayers (SLBs) integrated with various microscopy and electrochemical techniques were used to investigate the structure and function of MsbA. The results showed that electrochemical impedance spectroscopy (EIS) combined with biochemical detection could effectively monitor the activity of MsbA and potential inhibitors, providing a platform for the development of next-generation antimicrobials.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Multidisciplinary
Konstantinos Kallitsis, Anna-Maria Pappa, Zixuan Lu, Alberto Alvarez-Fernandez, Ioanna Charalambous, Sina Schack, Walther C. Traberg, Quentin Thiburce, Karan Bali, Graham Christie, Stefan Guldin, Susan Daniel, Alberto Salleo, Roisin M. Owens
Summary: This study presents a versatile method to modify the surface chemistry of conducting polymers, aiming to bridge the chemical gap between bioelectronic devices and biological systems. The surface of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) is modified to enhance the formation of cell-derived supported lipid bilayers (SLBs). The functionalized devices show improved electronic output and demonstrate the potential of this approach in membrane-on-a-chip systems.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Review
Materials Science, Multidisciplinary
Zixuan Lu, Aimie Pavia, Achilleas Savva, Loig Kergoat, Roisin M. Owens
Summary: Microelectrode arrays (MEAs) are devices used to record and stimulate biological activity of cells. Organic mixed ionic and electronic conductors (OMIECs) have gained attention as active materials for MEAs due to their advantages over traditional metal-based MEAs. These organic microelectrode arrays offer high signal-to-noise ratio and low electrochemical impedance, making them suitable for various applications in vivo and in vitro.
MATERIALS SCIENCE & ENGINEERING R-REPORTS
(2023)
Article
Engineering, Biomedical
Simone R. de Rijk, Alexander J. Boys, Iwan V. Roberts, Chen Jiang, Charlotte Garcia, Roisin M. Owens, Manohar Bance
Summary: Cochlear implants are revolutionary devices that partially restore hearing for those with severe sensorineural hearing loss. However, they can lead to fibrotic tissue formation in the cochlea, causing residual hearing loss and suboptimal outcomes. This study develops a tissue-engineered model to examine the electrical characteristics associated with fibrotic tissue formation.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Walther C. Traberg, Johana Uribe, Victor Druet, Adel Hama, Chrysanthi-Maria Moysidou, Miriam Huerta, Reece McCoy, Daniel Hayward, Achilleas Savva, Amaury M. R. Genovese, Suraj Pavagada, Zixuan Lu, Anil Koklu, Anna-Maria Pappa, Rebecca Fitzgerald, Sahika Inal, Susan Daniel, Roisin M. Owens
Summary: A functional phenotypic screening platform based on organic electrochemical transistors (OECTs) is developed for real-time, noninvasive monitoring of tumor-derived extracellular vesicle (TEV)-induced epithelial-to-mesenchymal transition (EMT) and screening of antimetastatic drugs. The TEVs derived from the triple-negative breast cancer cell line MDA-MB-231 induce EMT in nonmalignant breast epithelial cells (MCF10A), and heparin is identified as an effective blocker of TEV-induced EMT.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Karan Bali, Reece McCoy, Zixuan Lu, Jeremy Treiber, Achilleas Savva, Clemens F. Kaminski, George Salmond, Alberto Salleo, Ioanna Mela, Rita Monson, Roisin M. Owens
Summary: The use of phages as antibiotics has gained interest due to the declining effectiveness of conventional antibiotics. This study demonstrates the use of outer membrane vesicles (OMVs) derived from Gram-negative bacteria to create in vitro membrane models for detecting phage interactions. The findings show potential for identifying phages that can combat bacterial strains of interest.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
Roisin M. Owens
Summary: The FDA modernization act 2.0, signed into law by President Biden in December 2022, encourages the use of alternatives to animal testing for drug discovery. Cell-based assays are one important alternative, however they are currently not fit for purpose. The use of 3D, tissue engineered models represents a key development opportunity, to enable development of models of human tissues and organs.
MRS COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
William Etheridge, Frederic Brossard, Sitan Zheng, Svenja Moench, Suraj Pavagada, Roisin M. Owens, Ljiljana Fruk
Summary: By using polydopamine and gold nanoparticles, the reaction rate of copper-selective DNA cleaving DNAzyme can be significantly enhanced, making the combination of polydopamine and DNAzyme suitable for practical applications as a sensitive biosensor for Cu(ii) ions. Deposition of DNAzyme onto a gold electrode followed by Polydopamine Assisted DNA Immobilisation (PADI) allows for the development of a cost-effective, label-free, and fast electrochemical biosensor with a low limit of detection, thus paving the way for a new generation of hybrid DNAzyme-based biosensors.
Article
Chemistry, Multidisciplinary
Achilleas Savva, Janire Saez, Aimee Withers, Chiara Barberio, Verena Stoeger, Shani Elias-Kirma, Zixuan Lu, Chrysanthi-Maria Moysidou, Konstantinos Kallitsis, Charalampos Pitsalidis, Roisin M. Owens
Summary: Researchers have developed three-dimensional bioelectronic devices based on the electroactive polymer PEDOT:PSS for non-invasive electrical monitoring of stem cell growth. The properties and structure of the PEDOT:PSS scaffolds can be adjusted by changing processing parameters, allowing for support of stem cell cultures. The increase in cell population within the PEDOT:PSS network can be monitored by measuring the interface resistance, enabling the monitoring of stem cell proliferation.
MATERIALS HORIZONS
(2023)
Article
Chemistry, Multidisciplinary
Sarah L. Barron, Sophie V. Oldroyd, Janire Saez, Alice Chernaik, Wenrui Guo, Frank Mccaughan, David Bulmer, Roisin M. Owens
Summary: This study presents a flexible all-planar electronic device capable of monitoring barrier formation and perturbations in human respiratory and intestinal cells at the air liquid interface (ALI). By analyzing patient samples using electrochemical impedance spectroscopy and equivalent circuit models, disease-specific and patient-specific signatures are identified. The device's readouts are validated against commercially available chopstick electrodes and demonstrate superior conformability, sensitivity, and biocompatibility. This work lays the foundation for a physiologically relevant sensor capable of mapping local and real-time changes in epithelial barrier function at ALI, with broad applications in toxicology and drug screening.
ADVANCED MATERIALS
(2023)