Review
Plant Sciences
Peter Palfi, Laszlo Bakacsy, Henrietta Kovacs, Agnes Szepesi
Summary: Hypusination is a unique posttranslational modification of eIF5A that plays a crucial role in plant growth and stress response. This process, mediated by two enzymes, contributes to the diverse functions of eIF5A isoforms in signal pathways that can be modified effectively.
Review
Medicine, Research & Experimental
Jing-si Guo, Kai-li Liu, Yu -xi Qin, Lin Hou, Ling-yan Jian, Yue-hui Yang, Xin-yang Li
Summary: The discovery of new therapeutic strategies for diseases is crucial for drug research. DHPS plays a critical regulatory role in various diseases, and researchers are increasingly focusing on the development of DHPS inhibitors.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Helen Ashwin, Jovana Sadlova, Barbora Vojtkova, Tomas Becvar, Patrick Lypaczewski, Eli Schwartz, Elizabeth Greensted, Katrien Van Bocxlaer, Marion Pasin, Kai S. Lipinski, Vivak Parkash, Greg Matlashewski, Alison M. Layton, Charles J. Lacey, Charles L. Jaffe, Petr Volf, Paul M. Kaye
Summary: Leishmaniasis is considered a vaccine-preventable disease, but costs and uncertainty hinder progress in vaccine development. Controlled human infection models offer a way to speed up vaccine development and gain better understanding of disease pathogenesis.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Jianhui Zhang, Jiao Li, Kaifeng Hu, Qi Zhou, Xiaoxiao Chen, Jinlei He, Shuangshuang Yin, Yangjian Chi, Xuechun Liao, Yuying Xiao, Hanxiao Qin, Zhiwan Zheng, Jianping Chen
Summary: This study identified differentially expressed proteins in two Leishmania donovani strains using proteomics analysis and identified potential vaccine candidates through immunoinformatics analysis. The results suggest that these candidates have the potential for intervention against visceral leishmaniasis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ravi Ranjan Kumar, Ravi Jain, Sabir Akhtar, Nidha Parveen, Arabinda Ghosh, Veena Sharma, Shailja Singh
Summary: Vitamin B1 is essential for carbohydrate metabolism, and this study focuses on the enzyme Thiamine Pyrophosphokinase (TPK) and its role in Leishmania donovani, a parasite causing visceral leishmaniasis. In silico and biochemical experiments were conducted to identify and characterize LdTPK, and it was found that inhibiting LdTPK led to growth inhibition of the parasite. These findings suggest that LdTPK could be a potential target for the development of new anti-leishmanial agents.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Mohini Soni, Gaurava Srivastava, Karthik Ramalingam, Anil Kumar Shakya, Mohammad Imran Siddiqi, J. Venkatesh Pratap
Summary: Leishmaniasis, caused by Leishmania, is a disease affecting millions of people in endemic areas. Current therapies have limitations and the need for novel therapeutic molecules is urgent. In this study, structure-based high throughput drug discovery was used to identify two potential inhibitors of Leishmania HSK, which effectively induced mortality in the parasite and showed promising specificity and efficacy.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Madhusudhanarao Katiki, Monica Sharma, Neetu Neetu, Madhubala Rentala, Pravindra Kumar
Summary: This study aims to identify inhibitors against DOHH from Leishmania donovani for the treatment of Leishmaniasis. The LdDOHH protein was produced and characterized. Virtual screening identified folic acid, scutellarin, and homoarbutin as potential inhibitors, which were found to bind in the active site of LdDOHH and stabilize the structure.
BRIEFINGS IN FUNCTIONAL GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Mousumi Sinha, Rahul Jagadeesan, Neeraj Kumar, Satabdi Saha, Gugan Kothandan, Diwakar Kumar
Summary: The study aims to identify potential drugs against Ld-MIPS for preventing the spread of leishmaniasis. Through virtual screening and molecular docking, potential inhibitors with good pharmacokinetic properties were identified and further studied through molecular dynamics simulation.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Microbiology
Bart Cuypers, Pieter Meysman, Ionas Erb, Wout Bittremieux, Dirk Valkenborg, Geert Baggerman, Inge Mertens, Shyam Sundar, Basudha Khanal, Cedric Notredame, Jean-Claude Dujardin, Malgorzata A. Domagalska, Kris Laukens
Summary: Aneuploidy has a significant impact on the transcriptome, proteome, and metabolome of Leishmania donovani, leading to disruptions in stochiometric balances and metabolic differences between aneuploid strains. However, protein complex subunits and non-cytoplasmic proteins show attenuations in dosage effects. The differentially expressed proteins are enriched for those involved in protein metabolism. These findings suggest that aneuploidy in Leishmania can be adaptive and make it an attractive model for studying protein homeostasis in the context of aneuploidy.
Article
Immunology
Rahat Ali, Shams Tabrez, Sajjadul Kadir Akand, Fazlur Rahman, Atahar Husein, Mohd Arish, Ali S. Alqahtani, Mohammad Z. Ahmed, Mohammad Husain, Abdur Rub
Summary: This study evaluated the antileishmanial potential of sesamol against L. donovani, showing that sesamol inhibits parasite growth and proliferation through induction of reactive oxygen species (ROS), mitochondrial dysfunction, DNA fragmentation, cell cycle arrest, and apoptosis-like cell death. The results suggest the possible use of sesamol for the treatment of leishmaniasis following further in vivo validations.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Padmika Madushanka Wadanambi, Uthpali Mannapperuma
Summary: This computational study identified seven phytochemical inhibitors with strong binding affinity to the LdSQS enzyme, showing potential as antileishmanial drugs. These compounds demonstrated favorable physicochemical properties, pharmacokinetic profiles, and toxicity predictions, meeting in silico criteria for oral bioavailability.
Article
Immunology
Amrita Das, Mohd. Kamran, Nahid Ali
Summary: The study demonstrates that the liposomal formulation of HO-3867 is effective in inducing apoptosis in Leishmania parasites while being safe for normal murine macrophages. The treatment effectively reduces intracellular L. donovani amastigote burden by inducing parasite killing through ROS and NO activity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Infectious Diseases
Jairo Alfonso Mendoza-Roldan, Andrea Zatelli, Maria Stefania Latrofa, Roberta Iatta, Marcos Antonio Bezerra-Santos, Giada Annoscia, Floriana Gernone, Jan Votypka, David Modry, Lucie Ticha, Petr Volf, Domenico Otranto
Summary: This study confirms the circulation of L. infantum and L. tarentolae in sand flies, reptiles, and dogs. It also suggests that geckoes may be infected with L. infantum.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Kavita Vats, Rati Tandon, Roshanara, Mirza. A. Beg, Rosa M. Corrales, Akila Yagoubat, Enam Reyaz, Tasaduq. H. Wani, Mirza. S. Baig, Ashok Chaudhury, Anuja Krishnan, Niti Puri, Poonam Salotra, Yvon Sterkers, Angamuthu Selvapandiyan
Summary: This study investigates the function of Centrin4 and its binding protein LdPOC in the protozoan parasite Leishmania. The results show that Centrin4 interacts with LdPOC and localizes at the basal bodies, playing a role in parasite growth and cell division. This is the first report on the study of Centrin4 and its binding protein in protozoan parasites.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Myung Hee Park, Rajesh Kumar Kar, Siddharth Banka, Alban Ziegler, Wendy K. Chung
Summary: Hypusine, a derivative of lysine formed post-translationally in eukaryotic initiation factor 5A (eIF5A), plays a crucial role in various biological processes. Enzymes involved in hypusine synthesis, deoxyhypusine synthase and deoxyhypusine hydroxylase, exhibit strict specificity towards eIF5A substrates. eIF5A and the hypusine modification pathway are important in human neurodevelopment.
Article
Biochemistry & Molecular Biology
Reetika Manhas, Venkatraman Subramanian Gowri, Rentala Madhubala
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Biochemical Research Methods
Nidhi Tyagi, Edward J. Farnell, Colin M. Fitzsimmons, Stephanie Ryan, Edridah Tukahebwa, Rick M. Maizels, David W. Dunne, Janet M. Thornton, Nicholas Furnham
PLOS COMPUTATIONAL BIOLOGY
(2015)
Article
Cell Biology
Alok Kumar Singh, Rajeev Kumar Pandey, Chandrima Shaha, Rentala Madhubala
Article
Chemistry, Medicinal
Nitin A. Lunagariya, Vikrantsinh M. Gohil, Varun Kushwah, Soumya Neelagiri, Sanyog Jain, Sushma Singh, Kamlesh K. Bhutani
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2016)
Article
Genetics & Heredity
Neelagiri Soumya, Hitendra Tandan, Mangesh V. Damre, Rahul P. Gangwal, Abhay T. Sangamwar, Sushma Singh
Article
Biochemistry & Molecular Biology
Sneha Anand, Rentala Madhubala
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Parasitology
Neeradi Dinesh, Soumya Neelagiri, Vinay Kumar, Sushma Singh
EXPERIMENTAL PARASITOLOGY
(2017)
Article
Immunology
Vinay Kumar, Shailendra Yadav, Neelagiri Soumya, Rohit Kumar, Neerupudi Kishore Babu, Sushma Singh
MICROBIAL PATHOGENESIS
(2017)
Article
Biochemistry & Molecular Biology
Mahesh Sharma, Naeem Shaikh, Shailendra Yadav, Sushma Singh, Prabha Garg
MOLECULAR BIOSYSTEMS
(2017)
Article
Parasitology
Neelagiri Soumya, Mitesh N. Panara, Kishore Babu Neerupudi, Sushma Singh
PARASITOLOGY INTERNATIONAL
(2017)
Article
Infectious Diseases
Nimisha Mittal, Rohini Muthuswami, Rentala Madhubala
PLOS NEGLECTED TROPICAL DISEASES
(2017)
Article
Biochemistry & Molecular Biology
Vinay Kumar, Mahesh Sharma, Bhadoriya Ruchita Rakesh, Chandan Kumar Malik, Soumya Neelagiri, Kishore Babu Neerupudi, Prabha Garg, Sushma Singh
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Infectious Diseases
Sanya Chadha, Ramachandran Vijayan, Sakshi Gupta, Manoj Munde, Samudrala Gourinath, Rentala Madhubala
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Multidisciplinary Sciences
Preet Kamal Kaur, Neha Tripathi, Jayesh Desale, Soumya Neelagiri, Shailendra Yadav, Prasad V. Bharatam, Sushma Singh
Article
Infectious Diseases
Ruby Bansal, Shib Sankar Sen, Rohini Muthuswami, Rentala Madhubala
PLOS NEGLECTED TROPICAL DISEASES
(2019)