Review
Biochemistry & Molecular Biology
Awa Diop, Daniele Santorelli, Francesca Malagrino, Caterina Nardella, Valeria Pennacchietti, Livia Pagano, Lucia Marcocci, Paola Pietrangeli, Stefano Gianni, Angelo Toto
Summary: This review summarizes the latest research on the structure, function, pathogenic role, and regulation of SH2 domains. The findings suggest that pharmacological interest in SH2 domains and a deeper understanding of their binding properties are crucial.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chi-Chuan Lin, Kin Man Suen, Polly-Anne Jeffrey, Lukasz Wieteska, Jessica A. Lidster, Peng Bao, Alistair P. Curd, Amy Stainthorp, Caroline Seiler, Hans Koss, Eric Miska, Zamal Ahmed, Stephen D. Evans, Carmen Molina-Paris, John E. Ladbury
Summary: This study reveals a state of liquid-liquid phase separation that brings activated receptor tyrosine kinases (RTKs) to localized high concentrations of required proteins, overcoming the ambiguity of random diffusion. The study also discovers the formation process of phase-separated droplets comprising a ternary complex including RTK, phosphatase SHP2, and phospholipase PLC gamma 1, and identifies a previously undescribed interface. The complex combines kinase and phosphatase activities to control phosphorylation state and provides a scaffold for active PLC gamma 1 to facilitate access to its plasma membrane substrate. Thus, liquid-liquid phase separation modulates RTK signaling with potential consequences for therapeutic intervention.
Article
Cell Biology
Daniela Londono-Vasquez, Katherine Rodriguez-Lukey, Susanta K. Behura, Ahmed Z. Balboula
Summary: Through studying mouse oocytes, researchers discovered a subset of microtubule organizing centers called mcMTOCs that regulate the positioning of the spindle and faithful chromosome segregation during meiosis I. They found that mcMTOCs work in conjunction with F-actin to balance the forces exerted on the spindle and ensure its central anchoring and timely migration.
DEVELOPMENTAL CELL
(2022)
Article
Biochemistry & Molecular Biology
Jonathon A. Heier, Sabine Pokutta, Ian W. Dale, Sun Kyung Kim, Andrew P. Hinck, William Weis, Adam Kwiatkowski
Summary: The study reveals that alpha T-catenin has similar binding characteristics to alpha E-catenin but with differences in affinity and conformation when binding to vinculin. The N-terminus of alpha T-catenin is crucial in maintaining M-region autoinhibition and recruiting vinculin to cell-cell contacts.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Dan Zhang, Roxane P. Spiegelhalder, Emily B. Abrash, Tiago D. G. Nunes, Ines Hidalgo, M. Ximena Anleu Gil, Barbara Jesenofsky, Heike Lindner, Dominique C. Bergmann, Michael T. Raissig
Summary: In this study, we identified BdPOLAR as a novel factor involved in SC formation through comparative transcriptomics in Brachypodium distachyon. We found that BdPOLAR forms a distal polarity domain in subsidiary mother cells, opposite to the proximal PAN1 domain, and both polarity domains play important roles in coordinating the SC division process.
Article
Chemistry, Multidisciplinary
Changquan Tang, Xiaoling Ma, Jin-Yun Wang, Xue Zhang, Ruochuan Liao, Yunlong Ma, Peng Wang, Pengsong Wang, Tao Wang, Fujun Zhang, Qingdong Zheng
Summary: By utilizing different arrangements of selenophene heterocycles, the photovoltaic performance of various nonfullerene acceptors can be improved, resulting in increased efficiency.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Cell Biology
Mehrnaz Mehrabipour, Neda S. Kazemein Jasemi, Radovan Dvorsky, Mohammad R. Ahmadian
Summary: SRC homology 3 (SH3) domains are modular building blocks that facilitate the assembly of protein complexes through interactions with proline-rich/noncanonical motifs. They have been extensively studied across different life kingdoms and viruses, playing roles in various biological processes. Additionally, SH3 domains are implicated in the development of human diseases and are potential drug targets. This review summarizes the classification of SH3 domain functions, their binding specificity, and their significance in disease research.
Article
Oncology
Zhihan Li, Jinchuan Xi, Baokun Li, Youqiang Liu, Guiying Wang, Bin Yu, Hongqing Ma, Zhilin Li, Zhenya Zhang
Summary: This study found that low expression of SHP-2 may regulate M2 polarization of tumor-associated macrophages (TAMs) and exosome release through the PI3K/AKT pathway, thereby enhancing the migration and invasion ability of colorectal cancer (CRC) cells.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhonglei Bao, Jianghua Liu, Jin Fu
Summary: This study systematically created a comprehensive binary interaction map of all tau phosphotyrosine sites with SH2 domains in the human genome and identified strong and moderate binding pairs. The research found that the SH2-recognition specificity of different tau phosphotyrosine sites has been optimized evolutionarily and may regulate different but partially overlapped biological functions in tau signaling. Some SH2 groups, such as SRC, RIN, PLCG, SOCS, and SH2D, showed effective binding potency compared to others and could be potential tau-associated proteins in downstream signaling.
Article
Biochemistry & Molecular Biology
Jian Hu, Huili Su, Hui Cao, Hongbin Wei, Xiaokang Fu, Xuemei Jiang, Qin Song, Xinhua He, Changzheng Xu, Keming Luo
Summary: In poplar stems, the interaction between auxin and GA signaling pathways mediated by the RGL1-ARF7-IAA9 module is crucial for the precise regulation of cambial development.
Article
Biochemistry & Molecular Biology
Madison M. Mann, Min-Kang Hsieh, James D. Tang, William S. Hart, Matthew J. Lazzara, Jeffery B. Klauda, Bryan W. Berger
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates within intracellular membranes. The virus employs transmembrane 'accessory' proteins like ORF7a to interfere with the antiviral protein BST-2. Mutations in the transmembrane domain of BST-2, such as I28S, can alter its interactions with ORF7a. Through molecular dynamics simulations, specific interfaces and interactions between BST-2 and ORF7a in the transmembrane region were identified, providing a structural basis for their interactions.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Genetics & Heredity
Thomas E. Hughes, Olga Sedelnikova, Mimi Thomas, Jane A. Langdale
Summary: This study reveals the role of the SCARECROW gene in C-4 plants, showing that SCR and NAKED-ENDOSPERM together regulate the differentiation of internal leaf tissues. The authors found that SCR is involved in the patterning of internal leaf tissues in C-4 plants, with NKD enhancing its function. This study sheds light on the evolutionary process of cell patterning in grass leaves.