Article
Biochemistry & Molecular Biology
Yi Feng, Shaon Borosha, Anamika Ratri, Eun Bee Lee, Huizhen Wang, Timothy A. Fields, William H. Kinsey, Jay L. Vivian, M. A. Karim Rumi, Patrick E. Fields
Summary: Erythropoietin (EPO) signaling regulates calcium influx through TRPC2 and TRPC6 proteins in erythropoiesis. This study reveals that DOT1L regulates TRPC6 gene expression in erythroid progenitor cells, and loss of DOT1L leads to accelerated calcium influx through TRPC2 and detrimental effects on HPCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Julia A. Shevchenko, Kirill V. Nazarov, Alina A. Alshevskaya, Sergey V. Sennikov
Summary: Erythroid cells in the tumor microenvironment play a critical role in tumor growth and antitumor immune response, with tumor cells providing favorable conditions for the proliferation and survival of erythroid cells. Understanding these mechanisms can identify potential targets for immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Gui-Lan Chen, Bo Zeng, Hongni Jiang, Nikoleta Daskoulidou, Rahul Saurabh, Rumbidzai J. Chitando, Shang-Zhong Xu
Summary: An elevated level of circulating homocysteine is considered a risk factor for cardiovascular disease, but lowering therapy has unsatisfying clinical benefits. Researchers investigated the roles of calcium influx through TRPC channels and the regulation by Hcy-copper complexes. Hcy increased calcium influx in vascular endothelial cells through the activation of TRPC4 and TRPC5, which were regulated by extracellular divalent copper and Hcy. Hcy-copper complexes significantly regulated endothelial cell functions.
Article
Biochemistry & Molecular Biology
Naveed Aslam, Farah Alvi
Summary: Canonical transient receptor potential channels (TRPC3) play a crucial role in the development and viability of dendritic arbor in Purkinje neurons. By using a systems biology approach, we demonstrate that the activity of TRPC3 can be regulated by a Purkinje cell-specific local signalosome, contributing to the maintenance of Ca2+ and lipid signaling balance in Purkinje neurons.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biology
Chunling Xue, Xuechun Li, Li Ba, Yamei Shen, Zhao Sun, Junjie Gu, Ying Yang, Qin Han, Robert Chunhua Zhao
Summary: This study revealed the key role of TRPC3 in IRISIN-induced beiging of MSCs and provided a potential new target pathway for addressing metabolic disorders. The results also suggested that the interaction of irisin with TRPC3 may have effects on multiple tissues, including the brain.
Article
Oncology
Yasuyo Sano, Toshimi Yoshida, Min-Kyung Choo, Yanek Jimenez-Andrade, Kathryn R. Hill, Katia Georgopoulos, Jin Mo Park
Summary: Research has shown that tumor-promoting environmental stress and hemodynamic changes trigger erythropoietin production in the kidneys of mice, leading to the expansion of erythroid cell populations in the spleen. These erythroid cells exhibit specific molecular features and express genes encoding immune checkpoint molecules. Antibody-mediated erythropoietin blockade can reduce tumor growth, highlighting the potential of tumor-induced erythropoietin and erythroid cells as targets for cancer treatment.
MOLECULAR CANCER RESEARCH
(2021)
Article
Developmental Biology
Katja Eubler, Karolina M. Caban, Gregory A. Dissen, Ulrike Berg, Dieter Berg, Carola Herrmann, Nicole Kreitmair, Astrid Tiefenbacher, Thomas Frohlich, Artur Mayerhofer
Summary: This study discovered the expression of TRPV2 in the ovary and its involvement in inflammatory and immunological processes. The TRPV2 agonist CBD was found to induce Ca2+ fluxes and the production of inflammatory factors, as well as interfere with progesterone synthesis and alter the proteome and secretome.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Sek, Rafal P. Kampa, Bogusz Kulawiak, Adam Szewczyk, Piotr Bednarczyk
Summary: The study identified two populations of mitochondrial large-conductance Ca2+-regulated potassium channels in human bronchial epithelial cells and characterized their biophysical properties. The activity of the channels was modulated by potassium channel openers and inhibitors, suggesting a role in cell survival and death pathways. Additionally, the mitochondrial localization of alpha pore-forming and beta subunits of the BKCa channel was confirmed using RT-PCR and Western blot analysis.
Article
Multidisciplinary Sciences
Jade L. Taylor, Harry A. T. Pritchard, Katy R. Walsh, Patrick Strangward, Claire White, David Hill-Eubanks, Mariam Alakrawi, Grant W. Hennig, Stuart M. Allan, Mark T. Nelson, Adam S. Greenstein
Summary: The study investigates the role of vasodilatory pathways in cerebral arteries in the context of Alzheimer's disease. The researchers found that dysfunction in potassium channels involved in vasodilation may contribute to reduced blood flow and impaired functional hyperemia, highlighting the importance of targeting these pathways for potential therapeutic interventions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Wenjun Guo, Qinglin Tang, Miao Wei, Yunlu Kang, Jing-Xiang Wu, Lei Chen
Summary: In this study, the cryo-EM structures of TRPC3 and TRPC6 channels were determined, revealing the calcium-dependent regulation and the pathogenic mechanism of GOF mutations associated with diseases like FSGS. These findings provide important insights for further research and structure-based drug design.
Article
Cell Biology
Jian Li, Cheng Quan, Yun-Ling He, Yan Cao, Ying Chen, Yu-Fei Wang, Li-Ying Wu
Summary: This study reveals that hypoxia accelerates erythroid differentiation by enhancing autophagy through suppressing mTORC1 signaling. The upregulation of the HIF-1 target gene REDD1 is responsible for the suppression of mTORC1 signaling and enhancement of autophagy, promoting erythroid differentiation under hypoxic conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Takuto Fujii, Takahiro Shimizu, Mizuki Katoh, Shushi Nagamori, Keiichi Koizumi, Junya Fukuoka, Yoshiaki Tabuchi, Akira Sawaguchi, Tomoyuki Okumura, Kazuto Shibuya, Tsutomu Fujii, Hiroshi Takeshima, Hideki Sakai
Summary: The study reveals that Na+,K+-ATPase alpha 3-isoform is translocated from intracellular vesicles to the plasma membrane of detached cancer cells, contributing to their survival. Focal-adhesion-kinase-dependent Ca2+ response induces alpha 3NaK translocation, associated with activation of AMP-activated protein kinase.
Article
Pharmacology & Pharmacy
Shohei Yoshimoto, Miho Matsuda, Kenichi Kato, Eijiro Jimi, Hiroshi Takeuchi, Shuji Nakano, Shunichi Kajioka, Etsuko Matsuzaki, Takao Hirofuji, Ryuji Inoue, Masato Hirata, Hiromitsu Morita
Summary: The study found that VRAC is highly functional and associated with proliferation in HST-1 cells, but not in HaCaT cells. The pharmacological profile of VRAC in HST-1 was similar to previous reports, and inhibition of VRAC with DCPIB led to apoptosis in HST-1 cells. Knockdown of TMEM16A and LRRC8A attenuated VRAC activity in HST-1 cells.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Cell Biology
Tengteng Zhao, Sophia Parmisano, Zahra Soroureddin, Manjia Zhao, Lauren Yung, Patricia A. Thistlethwaite, Ayako Makino, Jason X. -J Yuan
Summary: TRPC6 and Piezo1 channels both contribute to the increase in intracellular calcium concentration in pulmonary arterial endothelial cells (PAECs), with TRPC6 being sensitive to diacylglycerol and Piezo1 being sensitive to membrane stretch.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Editorial Material
Cell Biology
Shiyan Liu, Mutian Chen, Yichang Wang, Huihui Li, Shiqian Qi, Jia Geng, Kefeng Lu
Summary: The ER calcium channel Csg2 regulates sphingolipid metabolism through controlling ER calcium homeostasis and affects cellular death. Deletion of Csg2 leads to an increase in ER calcium concentration, destabilizing the sphingolipid synthase Aur1 and resulting in the accumulation of the bioactive sphingolipid phytosphingosine (PHS), which blocks autophagy. This work highlights the connection between ER calcium homeostasis, sphingolipid metabolism, and autophagy initiation.
Article
Engineering, Aerospace
Xingyu Zhou, Tong Qin, Mingjiang Ji, Dong Qiao
Summary: This paper proposes a novel framework for efficiently solving continuously maneuvering spacecraft orbit determination (OD) problems. It combines the Long Short-Term Memory (LSTM) neural network and filter algorithms. The LSTM is trained to detect unknown maneuvers and estimate coefficients of a polynomial representation for a more accurate estimation. The framework is successfully applied to solve Low-Earth-orbit and Middle-Earth-orbit OD problems, accurately tracking maneuvering targets and estimating unknown maneuvers. Numerical simulations show the LSTM can be applied to scenarios with common features to the training dataset.
Article
Automation & Control Systems
Rui Na, Ji-An Pan, Jialing Zhou, Yuezu Lv, Tong Qin, Dezhi Zheng
Summary: This paper investigates the mean square consensus problem of a class of nonlinear networked systems under directed and stochastic switching communication topologies, with the switching law determined by an ergodic continuous-time Markov process. An observer-based method is used to design the cooperative consensus controller. The paper proves that the mean square consensus can be achieved if the union of the underlying network graphs has a directed spanning tree, even if none of the network graphs contain a directed spanning tree. The effectiveness of the proposed controllers is illustrated through a numerical simulation on multiple Chua's circuit systems.
JOURNAL OF THE FRANKLIN INSTITUTE-ENGINEERING AND APPLIED MATHEMATICS
(2023)
Article
Veterinary Sciences
Tong Qin, Mingjie Shi, Meina Zhang, Zhitong Liu, Hao Feng, Yi Sun
Summary: Metagenomic sequencing was used to investigate the viromes in three tick species (Haemaphysalis concinna, Dermacentor silvarum, and Ixodes persulcatus) in North China. A total of 28 RNA viruses belonging to different viral families were identified. Ixodes persulcatus ticks had a higher diversity of viral species compared to the other two tick species. The study highlights the importance of studying tick-borne viruses for viral taxonomy and understanding tick-transmitted viral zoonotic diseases.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Chemistry, Medicinal
Tong Qin, Xuefeng Gao, Lei Lei, Jing Feng, Wenxuan Zhang, Yuhua Hu, Zhufang Shen, Zhenming Liu, Yi Huan, Song Wu, Jie Xia, Liangren Zhang
Summary: In this study, a novel potential drug target for nonalcoholic fatty liver disease (NAFLD) named Farnesoid X receptor (FXR) agonist was discovered through the development of a multi-stage computational workflow. The compound identified as the representative showed potent FXR agonistic activity in HEK293T cells and demonstrated positive effects on hypercholesterolemia, hepatic steatosis, hyperglycemia, and insulin resistance (IR) in high-fat-diet induced obese (DIO) mice.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Qihong Yang, Tong Qin, Tao An, Hongna Wu, Gang Xu, Jin Xiang, Kangfan Lei, Shaohua Zhang, Jie Xia, Guifeng Su, Dan Wang, Minggao Xue, Lingmei Kong, Wenxuan Zhang, Song Wu, Yan Li
Summary: In this study, researchers designed a series of pyridonyl acetamide compounds and discovered a novel PORCN inhibitor WHN-88 with a unique di-iodinated pyridone structural fragment. WHN-88 effectively abolished palmitoylation of Wnt ligands and prevented their secretion and the subsequent Wnt/13-catenin signaling transduction. Furthermore, WHN-88 demonstrated potent efficacy against Wnt-driven cancers and inhibited cancer cell stemness.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Shangwen Liu, Shijie Zhou, Hao Guan, Qian-Ming Zhang, Tong Qin, Jiarong Lin
Summary: This paper provides a comprehensive analysis of the relationship between customers' quality preference for product-service, R&D investment, and product-service price. The results show that an enterprise's absorptive capacity positively influences market demand and profit, while negatively impacting competitors. A higher customers' quality preference coefficient indicates a stronger competitive advantage. Implementing a non-cooperative R&D strategy helps enterprises expand their R&D investment scale and accumulate knowledge and technology, leading to increased sales volume and profits. The paper suggests that enterprises should focus on improving absorptive capacity, diversifying R&D strategy, and considering customers' quality preferences and market demand when making R&D investment decisions.
Article
Chemistry, Physical
Sen Liang, Ting Cai, Jing Yuan, Qin Tong, Xiaojun Hu
Summary: Co-based catalyst is an effective catalyst for degrading VC due to its excellent chlorine resistance. High-performance CoxBa1 catalysts were prepared by doping Ba into Co3O4 and changing the Ba/Co mol ratio. The addition of BaCO3 in CoxBa1 reduces its particle size, increases the active site, and improves its low-temperature reducibility. Furthermore, BaCO3 increases the basic sites of CoxBa1, which effectively protects Co3O4 from Cl poisoning. Co19Ba1, with the highest BaCO3 content at Ba/Co 1/19, exhibits the best catalytic activity.
CHEMICAL PHYSICS IMPACT
(2023)
Article
Engineering, Aerospace
Xingyu Zhou, Tong Qin, Malcolm Macdonald, Dong Qiao
Summary: This paper performs an observability analysis for the inertial angle-only cooperative OD system. It finds that previous conclusions do not hold for some special configurations. Eight special configurations are proposed, and the unobservable orbit elements are revealed by analyzing the linear relationship among different columns of the observability matrix. The results show the relationship between observability and convergence ratio of estimations and provide a reference for the design and management of the constellation.
Article
Computer Science, Interdisciplinary Applications
Tong Qin, Ruxu Du, Yong Zhong
Summary: In the past few years, the global manufacturing industry has undergone significant changes due to various man-made and natural disasters. This paper presents a new method for designing resilient Flexible Manufacturing System (FMS) to counter and prevent losses. The resilience of FMS is measured through production loss and investment return, and the method involves two strategies: using Flexible Manufacturing Cells (FMC) and reconfiguring routes (Strategy A), or using a combination of FMC and extended storage capacity (Strategy B). The effectiveness of the method is demonstrated through a case study.
COMPUTERS & INDUSTRIAL ENGINEERING
(2023)
Article
Chemistry, Analytical
Huihui Shao, Jing Feng, Hanyilan Zhang, Yuanyuan Zhang, Tong Qin, Yuhua Hu, Wenxuan Zhang, Tiesong Wang, Song Wu, Qingyun Yang
Summary: In this study, the development and validation of reliable and selective HPLC and HPLC-MS/MS methods for the quantitative analysis of impurities in the drug IMM were described. Five minor impurities were identified and analyzed using spectroscopic evidence, chemical synthetic methods, and LC-MS/MS. The methods showed satisfactory linearity, precision, and repeatability, and the recommended procedures were applied to the existing active pharmaceutical ingredients of IMM, generating satisfactory results.
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2023)
Article
Veterinary Sciences
Ying Liu, Yanlong Su, Zhijie Zhou, Jie Zhu, Qianqian Zhu, Peng Xie, Shiquan Qian, Liwei Wang, Tong Qin, Gang Zhou
Summary: The study investigated the immune activity and molecular mechanisms related to the hygiene hypothesis in Meishan pigs raised under normal captivity or free-range conditions. Significant differences in cytokine levels and gene expression profiles were observed between the two groups. The identification of specific genes and metabolites associated with immune-related pathways provides new insight into the regulation of cellular immunity and the hygiene hypothesis.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Robotics
Tong Qin, Haihui Huang, Ziqiang Wang, Tongqing Chen, Wenchao Ding
Summary: An accurate road topological structure is crucial for autonomous driving in complex urban environments. Currently, a High-Definition map (HD map) is heavily relied upon by most autonomous vehicles to navigate through the city. However, producing such a map manually is time-consuming and prone to errors. In this letter, a framework is proposed to automatically generate the topological map of complicated intersections using crowd-sourced semantic information about the environment and traffic flows. This highly automatic and scalable framework speeds up HD map production and reduces costs, with results comparable to traditional HD maps validated by real-world crowdsourcing data.
IEEE ROBOTICS AND AUTOMATION LETTERS
(2023)
Article
Veterinary Sciences
Yinghao Wu, Guangzhi Zhang, Hui Jiang, Ting Xin, Li Jia, Yichen Zhang, Yifei Yang, Tong Qin, Chuang Xu, Jie Cao, Gobena Ameni, Arfan Ahmad, Jiabo Ding, Limin Li, Yuzhong Ma, Xuezheng Fan
Summary: This study isolated and identified nine strains of BVDV from persistently infected cattle in China. The phylogenetic analysis showed that these new isolates were classified as the BVDV-1b subgenotype, sharing high homology with the CP7 strain. In addition, some genome variations and unique amino acid mutations were found in these new isolates. Further research on the virulence and pathogenesis of these new strains is recommended.
VETERINARY SCIENCES
(2023)
Article
Chemistry, Organic
Min Zeng, Fuli Huang, Minghui Zhu, Jichao Ding, Tong Qin, Maoting Xu, Wanqing Liu, Jun Lu, Jicheng Wu, Xurong Qin, Qiao Ren
Summary: Despite the availability of chemical feedstocks, developing useful cross-coupling reactions with methyl esters as acyl electrophiles is still challenging due to their chemical inertness. We present a simple nickel-catalyzed intramolecular alkene hydroacylation method for the efficient synthesis of various medicinally active indanone and indazolone derivatives. This approach offers good functional group tolerance, easy post-synthetic modifications, total synthesis, and fluorescent probe synthesis. Preliminary in vitro antioxidant evaluation reveals promising free radical scavenging activities of 2-(4-dimethylaminophenyl)-1-indanone and 2-aryl-3-indazolone derivatives.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Correction
Materials Science, Composites
C. Li, J. Huang, T. Qin, C. Chen, L. Gao, J. Xu
JOURNAL OF COMPOSITE MATERIALS
(2023)
