Article
Biotechnology & Applied Microbiology
Aroa Ferrer-Pinos, Victor Garrigos, Emilia Matallana, Agustin Aranda
Summary: Wine strains of Saccharomyces cerevisiae need to adapt their metabolism to different growth conditions during biotechnological applications, with the transcription factor Gln3 playing a crucial role in glucose and nitrogen repression. Commercial yeast strains exhibit a variety of glucose repression profiles, and deletion of the GLN3 gene results in different mutant phenotypes. The nitrogen catabolite repression controlled by TORC1 is largely conserved in winemaking conditions, with mutants showing increased tolerance to rapamycin inhibition of nutrient signaling complex TORC1.
FERMENTATION-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Francisco Gutierrez-Santiago, Francisco Navarro
Summary: Ribosomes are vital for protein production, and their biogenesis is regulated by cellular energy status and stress signals. In eukaryotic cells, stress signals and newly-synthesized ribosomes require transcription by RNA polymerases. The Target of Rapamycin pathway in eukaryotes influences RNA polymerase transcription to ensure proper ribosome production. This review focuses on how TOR regulates transcription in Saccharomyces cerevisiae and the coordination of three RNA polymerases.
Article
Biotechnology & Applied Microbiology
Ya-Ping Wang, Lin Liu, Xue-Shan Wang, Kun-Qiang Hong, Li-Hua Zhang, Zhong-Guan Sun, Dong-Guang Xiao
Summary: The study shows that the GAT1 gene plays a crucial role in regulating higher alcohol production in Saccharomyces cerevisiae, with its deletion leading to decreased levels of higher alcohols. The downregulation of specific genes is partly responsible for this effect. These findings provide important insights for the wheat beer industry, offering valuable guidance for production and future research.
BIOENGINEERING-BASEL
(2021)
Article
Genetics & Heredity
Francisco Gutierrez-Santiago, Maria Cintas-Galan, Manuel Martin-Exposito, Maria del Carmen Mota-Trujillo, Cristina Cobo-Huesa, Jorge Perez-Fernandez, Francisco Navarro Gomez
Summary: Bud27 is a prefoldin-like protein that associates with RNA polymerases and plays a role in gene expression and ribosome biogenesis through the TOR signaling cascade. It is also involved in maintaining cell wall integrity and may impact autophagy induction.
Article
Biochemistry & Molecular Biology
Yuko Ishino, Nao Komatsu, Ken-taro Sakata, Daichi Yoshikawa, Motohiro Tani, Tatsuya Maeda, Kanta Morishige, Koushiro Yoshizawa, Naotaka Tanaka, Mitsuaki Tabuchi
Summary: The study revealed that the regulatory subunit encoded by the yeast LIP1 gene can form a complex with the catalytic subunits of ceramide synthase to complete the sphingolipid biosynthetic pathway. Replacing the LIP1 promoter leads to growth inhibition and decreased sphingolipid synthesis in yeast. Experimental results showed that this change has an impact on TORC2-Ypk1 signaling.
Article
Cell Biology
Yongook Lee, Bongkeun Kim, Hae-Soo Jang, Won-Ki Huh
Summary: Macroautophagy/autophagy is a crucial degradation pathway that cells use to maintain homeostasis and survive under stressful conditions. It has been discovered that Atg1-dependent phosphorylation of Vps34 is necessary for robust autophagy activity in yeast. This phosphorylation occurs at multiple serine/threonine residues in the helical domain of Vps34 in complex I and is essential for autophagy activation and cell survival. Furthermore, the localization of Vps34 complex I to the phagophore assembly site (PAS) is responsible for the complex I-specific phosphorylation of Vps34 and affects the dynamics of Atg18 and Atg8 at the PAS.
Article
Microbiology
Xinchao Yang, Jiadong Huang, Yuling Yang, Chunjiang Ye, Yuanxiu Wang, Yuehui Liu, Fang Wang, Naxin Sun, Weiping Yu
Summary: This study evaluated the effect of different nitrogen sources on the growth and fermentative performance of S. cerevisiae in cassava-ethanol fermentation. It was found that using urea and ammonium acetate as nitrogen sources reduced fermentation time by 19% and led to a higher alcohol yield compared to other nitrogen sources. The results also showed that ammonium acetate not only increased ethanol generation but also decreased glycerol generation. The study provides valuable insights for developing new strategies to control fermentation performance.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jose Manuel Villalba, Placido Navas
Summary: Coenzyme Q, a crucial molecule in aerobic metabolism, is involved in multiple metabolic pathways and mitochondrial functions. Its biosynthesis and redox state alterations can lead to mitochondrial dysfunctions and impact various disorders. Additionally, there is a bidirectional relationship between CoQ and the epigenome, further adding complexity to the regulatory pathways of CoQ levels.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Zhi-Jiao Sun, Jia-Zhang Lian, Li Zhu, Yi-Qi Jiang, Guo-Si Li, Hai-Long Xue, Mian-Bin Wu, Li-Rong Yang, Jian-Ping Lin
Summary: This study successfully improved the production of ergosterol in yeast by modifying the biosynthetic pathway of ergosterol and enhancing storage capacity. Dynamic control of gene expression using auto-inducible promoters further increased the ergosterol content in yeast.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Jennifer J. Tate, Rajendra Rai, Claudio De Virgilio, Terrance G. Cooper
Summary: Gln3 activates Nitrogen Catabolite Repression to help Saccharomyces cerevisiae scavenge poor nitrogen sources. Its nuclear localization is regulated by the TorC1 kinase complex, with the C-terminal Gln3-Tor1 interaction site playing a vital role. The N- and C-terminal Gln3-Tor1 interaction sites function autonomously and collaboratively to achieve Gln3 nuclear localization.
Article
Biotechnology & Applied Microbiology
Ai-Ping Pang, Funing Zhang, Xin Hu, Yongsheng Luo, Haiyan Wang, Samran Durrani, Fu-Gen Wu, Bing-Zhi Li, Zhihua Zhou, Zuhong Lu, Fengming Lin
Summary: This study investigated the impact of different nitrogen sources on cellulase biosynthesis in Trichoderma reesei, revealing glutamine as a significant inhibitor and its effect on gene expression related to nitrogen metabolism. These findings enhance our understanding of nitrogen regulation of cellulase production in fungi.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Food Science & Technology
Shuangping Liu, Donglin Ma, Zhihui Li, Hailong Sun, Jieqi Mao, Ying Shi, Xiao Han, Zhilei Zhou, Jian Mao
Summary: This study assessed the types and amounts of YAN sources and genes regulating higher alcohol synthesis in a yeast used for huangjiu brewing. It found that NH4Cl was most effective in reducing higher alcohol content, providing insights for controlling huangjiu quality by adding NH4Cl.
Article
Microbiology
Tianyu Zhao, Asaka Chida, Yuichi Shichino, Dongwoo Choi, Masaki Mizunuma, Shintaro Iwasaki, Yoshikazu Ohya
Summary: This study reveals the global translational regulation during replicative aging in yeast using ribosome profiling technique. The research found that the translational efficiency of genes involved in DNA repair and chromosome organization increased, while the translational efficiency of genes encoding ribosome components, transposons and cell wall assembly decreased during replicative aging.
Article
Biology
Rosemary Yu, Egor Vorontsov, Carina Sihlbom, Jens Nielsen
Summary: The study revealed that cell growth rate coordinately controls transcription and translation through RNA polymerase II and ribosome abundance, while nitrogen metabolism independently controls them through amino acid and nucleotide availabilities. Genes in central carbon metabolism are distinctly regulated compared to other genes in response to cell growth rate or nitrogen metabolism.
Article
Genetics & Heredity
Rajendra Rai, Jennifer J. Tate, Karthik Shanmuganatham, Martha M. Howe, David Nelson, Terrance G. Cooper
Article
Biochemistry & Molecular Biology
Georis Isabelle, Jennifer J. Tate, Fabienne Vierendeels, Terrance G. Cooper, Evelyne Dubois
Article
Genetics & Heredity
Jennifer J. Tate, Isabelle Georis, Rajendra Rai, Fabienne Vierendeels, Evelyne Dubois, Terrance G. Cooper
G3-GENES GENOMES GENETICS
(2015)
Article
Genetics & Heredity
Jennifer J. Tate, David Buford, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Rajendra Rai, Jennifer J. Tate, Terrance G. Cooper
G3-GENES GENOMES GENETICS
(2016)
Article
Genetics & Heredity
Jennifer J. Tate, David Buford, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Jennifer J. Tate, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Jennifer J. Tate, Rajendra Rai, Terrance G. Cooper
Article
Genetics & Heredity
Jennifer J. Tate, Rajendra Rai, Terrance G. Cooper
Article
Biochemistry & Molecular Biology
Andre Feller, Isabelle Georis, Jennifer J. Tate, Terrance G. Cooper, Evelyne Dubois
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Rajendra Rai, Jennifer J. Tate, David R. Nelson, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Rajendra Rai, Jennifer J. Tate, Isabelle Georis, Evelyne Dubois, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Jennifer J. Tate, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Rajendra Rai, Jennifer J. Tate, Karthik Shanmuganatham, Martha M. Howe, Terrance G. Cooper
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Microbiology
Mohammad Fayyadkazan, Jennifer J. Tate, Fabienne Vierendeels, Terrance G. Cooper, Evelyne Dubois, Isabelle Georis