Article
Biochemistry & Molecular Biology
Meng Wang, Edmund Cotter, Ya-Juan Wang, Xu Fu, Angela L. Whittsette, Joseph W. Lynch, R. Luke Wiseman, Jeffery W. Kelly, Angelo Keramidas, Ting-Wei Mu
Summary: This study demonstrates that pharmacologically enhancing endoplasmic reticulum (ER) proteostasis can increase the assembly, trafficking, and surface expression of variant GABA(A) receptors, offering a potential opportunity to mitigate pathology associated with genetic epilepsy and other disease-associated variant ion channels.
CELL AND BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ya-Juan Wang, Xiao-Jing Di, Ting-Wei Mu
Summary: This study identified the interactomes of GABAA receptors and revealed potential components and pathways involved in the biogenesis and degradation of GABAA receptors. TRIM21 was found to positively regulate the degradation of misfolding-prone GABAA receptor subunits. This research provides insights into the molecular mechanisms of GABAA receptor proteostasis and offers possibilities for the treatment of neurological diseases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Kenichiro Sadamitsu, Leona Shigemitsu, Marina Suzuki, Daishi Ito, Makoto Kashima, Hiromi Hirata
Summary: The study cloned the genes of zebrafish GABA(A) receptor subunits, investigated their expression patterns and electrophysiological properties, revealing that the functional characteristics of zebrafish GABA(A) receptors are conserved among vertebrates.
SCIENTIFIC REPORTS
(2021)
Article
Medicine, Research & Experimental
Yuki Sasaki, Hideki Arimochi, Kunihiro Otsuka, Hiroyuki Kondo, Shin-ichi Tsukumo, Koji Yasutomo
Summary: Immunoproteasome dysfunction leads to proteasome-associated autoinflammatory syndromes. In a mouse model, Psmb8 mutation causes hyperactivation of the CXCR3 pathway, resulting in increased susceptibility to skin inflammation.
Article
Biochemistry & Molecular Biology
Ya-Juan Wang, Xiao-Jing Di, Ting-Wei Mu
Summary: This study systematically identified the interactomes of GABA(A) receptors using a proteomics-based approach. The findings shed light on the proteostasis network components involved in GABA(A) receptor biogenesis and suggest a potential regulatory mechanism for the degradation of misfolding-prone subunits.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Clinical Neurology
Linlin Zhang, Xinjie Liu
Summary: De novo pathogenic variants in GABRA1 are more common than inherited variants, and most children with epilepsy show symptoms within the first year of life, presenting with a variety of seizure types and developmental delays.
FRONTIERS IN NEUROLOGY
(2022)
Review
Neurosciences
Yu Sun, Zechun Peng, Xiaofei Wei, Nianhui Zhang, Martin Wallner, Istvan Mody, Carolyn R. Houser
Summary: The decreased expression of the delta subunit of the GABA(A) receptor has been found to be associated with altered modulation of tonic inhibition in dentate granule cells in animal models of epilepsy and other disorders. This study aimed to investigate if viral transfection of delta subunits could restore network excitability in a mouse model of epilepsy. The results showed that transfection of delta subunits increased delta subunit expression, downregulated other GABA(A) receptor subunits, and restored more normal network excitability.
PROGRESS IN NEUROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jeong Joo Kim, Ryan E. Hibbs
Summary: GABAA receptors are important in mediating fast neuronal inhibition in the brain and have rich pharmacology. Recent efforts in structural biology have provided insights into how important drug classes and natural products interact with these receptors, laying a foundation for drug design. The focus has been on subunit assemblies of the receptor found at synapses.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Veterinary Sciences
Enrice I. Huenerfauth, Christian G. Bien, Corinna Bien, Holger A. Volk, Nina Meyerhoff
Summary: This case report describes the first encephalitis associated with antibodies against the gamma-aminobutyric acid-A receptor (GABA(A)R) in a dog, with a good outcome following immunotherapy.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jing Liu, Pingyang Ke, Haokun Guo, Juan Gu, Yan Liu, Xin Tian, Xuefeng Wang, Fei Xiao
Summary: A study found that Toll-like receptor 7 (TLR7) is activated in the early stage of epileptogenesis and affects epileptogenesis by regulating autophagy and inhibitory synaptic transmission. Activation of TLR7 induces autophagy and decreases the expression of KIF5A, which in turn influences abnormal GABA(A)R-mediated postsynaptic transmission. The results indicate that TLR7 is an important factor in regulating epileptogenesis and may serve as a potential therapeutic target for epilepsy.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Simone M. Braendle, Manuela Cerina, Susanne Weber, Kathrin Held, Amelie F. Menke, Carmen Alcala, David Gebert, Alexander M. Herrmann, Hannah Pellkofer, Lisa Ann Gerdes, Stefan Bittner, Frank Leypoldt, Bianca Teegen, Lars Komorowski, Tania Kuempfel, Reinhard Hohlfeld, Sven G. Meuth, Bonaventura Casanova, Nico Melzer, Eduardo Beltran, Klaus Dornmair
Summary: Encephalitis associated with antibodies against the neuronal GABA(A)-R is a rare form of autoimmune encephalitis. A study found a significantly expanded B cell clone in the cerebrospinal fluid of a patient with GABA(A)-R encephalitis, producing antibodies that likely contribute to clinical disease symptoms. Additionally, cross-reactivity between GABA(A)-R and the protein LMO5 was identified, supporting the hypothesis of a paraneoplastic etiology in GABA(A)-R encephalitis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Richard H. Kramer, Rajit Rajappa
Summary: To better understand neural circuits and behavior, scientists have developed microbial opsins as optogenetic tools for precise stimulation or inhibition of action potentials. Additionally, they have also developed light-regulated neurotransmitter receptors to perturb synaptic function at high resolution.
CURRENT OPINION IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Neus Pedraza, Ma Ventura Monserrat, Francisco Ferrezuelo, Jordi Torres-Rosell, Neus Colomina, Federico Miguez-Cabello, Javier Picanol Parraga, David Soto, Esperanza Lopez-Merino, Celia Garcia-Vilela, Jose A. Esteban, Joaquim Egea, Eloi Gari
Summary: In this study, Nuclear Cyclin D1 is found to interact with and phosphorylate the α4 subunit of GABA A receptors, increasing the surface levels of α4-containing GABA A receptors and enhancing their response. This molecular link between D1-Cdk4 and GABA A receptors activity highlights a novel role for this G1 cyclin in neuronal signalling.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Neurosciences
Ruotong Li, Yong Wang, Yaxin Yang, Zhongheng Wu, Ling Wang, Guoyi Tang, Jie Yang, Jian Liu
Summary: This study found that GABAA receptor-mediated inhibitory transmission in the avBNST is involved in Parkinson's disease (PD)-related anxiety. Injection of GABAA receptor agonist produced anxiolytic-like effects, inhibited the firing activity of GABAergic neurons and activated the dopaminergic neurons in VTA and serotonergic neurons in DRN, leading to an increase in dopamine and 5-HT release in the amygdala.
Article
Multidisciplinary Sciences
Shaotong Zhu, Akshay Sridhar, Jinfeng Teng, Rebecca J. Howard, Erik Lindahl, Ryan E. Hibbs
Summary: GABA(A) receptors are important drug targets in the central nervous system for treating anxiety, sleep disorders, and epilepsy. This study presents two high-resolution structures of GABA(A) receptors bound by the drugs zolpidem and DMCM, providing insights into receptor modulation.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Mark W. Irvine, Guangyu Fang, Kiran Sapkota, Erica S. Burnell, Arturas Volianskis, Blaise M. Costa, Georgia Culley, Graham L. Collingridge, Daniel T. Monaghan, David E. Jane
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Neurosciences
Pojeong Park, Thomas M. Sanderson, Zuner A. Bortolotto, John Georgiou, Min Zhuo, Bong-Kiun Kaang, Graham L. Collingridge
Editorial Material
Cell Biology
Thomas M. Sanderson, John Georgiou, Patrick Tidball, Graham L. Collingridge
Article
Behavioral Sciences
Enoch Ng, John Georgiou, Ariel Avila, Kathleen Trought, Ho-Suk Mun, Meggie Hodgson, Panayiotis Servinis, John C. Roder, Graham L. Collingridge, Albert H. C. Wong
BEHAVIOURAL BRAIN RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Thomas Gonatopoulos-Pournatzis, Rieko Niibori, Eric W. Salter, Robert J. Weatheritt, Brian Tsang, Shaghayegh Farhangmehr, Xinyi Liang, Ulrich Braunschweig, Jonathan Roth, Shen Zhang, Tyler Henderson, Eesha Sharma, Mathieu Quesnel-Vallieres, Jon Permanyer, Stefan Maier, John Georgiou, Manuel Irimia, Nahum Sonenberg, Julie D. Forman-Kay, Anne-Claude Gingras, Graham L. Collingridge, Melanie A. Woodin, Sabine P. Cordes, Benjamin J. Blencowe
Article
Multidisciplinary Sciences
Jue Xiang Wang, Mark W. Irvine, Erica S. Burnell, Kiran Sapkota, Robert J. Thatcher, Minjun Li, Noriko Simorowski, Arturas Volianskis, Graham L. Collingridge, Daniel T. Monaghan, David E. Jane, Hiro Furukawa
NATURE COMMUNICATIONS
(2020)
Article
Neurosciences
Mascia Amici, Yeseul Lee, Robert J. P. Pope, Clarrisa A. Bradley, Adam Cole, Graham L. Collingridge
Summary: Deregulation of GSK-3 beta is closely related to various serious brain conditions, and understanding its physiological functions in the central nervous system is crucial for disease research. Studies have shown that GSK-3 beta stabilizes synaptic NMDA receptors by phosphorylating PI4KII alpha, thereby controlling synaptic transmission.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Review
Neurosciences
Thomas M. Sanderson, John Georgiou, Graham L. Collingridge
FRONTIERS IN NEURAL CIRCUITS
(2020)
Article
Neurosciences
Thomas T. Luyben, Jayant Rai, Hang Li, John Georgiou, Ariel Avila, Mei Zhen, Graham L. Collingridge, Takashi Tominaga, Kenichi Okamoto
FRONTIERS IN NEURAL CIRCUITS
(2020)
Article
Multidisciplinary Sciences
Pojeong Park, John Georgiou, Thomas M. Sanderson, Kwang-Hee Ko, Heather Kang, Ji-il Kim, Clarrisa A. Bradley, Zuner A. Bortolotto, Min Zhuo, Bong-Kiun Kaang, Graham L. Collingridge
Summary: Long-term potentiation at hippocampal CA1 synapses can be achieved by increasing the number and/or single-channel conductance of AMPA receptors, with CaMKII and PKA working together to increase single-channel conductance.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Pojeong Park, Heather Kang, John Georgiou, Min Zhuo, Bong-Kiun Kaang, Graham L. Collingridge
Summary: The study demonstrates that calcium-permeable AMPA receptors are crucial for heterosynaptic metaplasticity. Results show that significant metaplasticity occurs when a strong TBS precedes a weak TBS, and pausing stimulation can largely eliminate this metaplasticity.
Article
Orthopedics
Rahul Gawri, Ryszard Bielecki, Eric W. Salter, Alena Zelinka, Toshikazu Shiba, Graham Collingridge, Andras Nagy, Rita A. Kandel
Summary: Inorganic polyphosphates (polyP) require calcium influx to regulate chondrocyte matrix production, likely through activating calcium signaling pathway. These findings help in the clinical translation of polyP into a therapeutic agent for cartilage repair.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Endocrinology & Metabolism
Matthew T. Birnie, Alen Eapen, Yvonne M. Kershaw, David Lodge, Graham L. Collingridge, Becky L. Conway-Campbell, Stafford L. Lightman
Summary: More than 50% of depressed patients exhibit hyperactivity of the hypothalamic-pituitary-adrenal (HPA) axis. Ketamine, a rapid-acting antidepressant, has a significant impact on HPA axis function. The timing of ketamine administration may be crucial for its glucocorticoid-mediated action in depression.
JOURNAL OF NEUROENDOCRINOLOGY
(2022)
Article
Chemistry, Organic
Lisa-Maria Recnik, Robert J. Thatcher, Shahida Mallah, Craig P. Butts, Graham L. Collingridge, Elek Molnar, David E. Jane, Christine L. Willis
Summary: (-)-Arctigenin and new analogues showed antagonist activity at both AMPA and kainate receptors, with spirocyclic analogue 6c acting as a non-competitive antagonist. Molecular docking studies suggest they bind in a similar manner to known AMPA receptor antagonists, indicating potential as novel leads for epilepsy treatment drug development.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Neurosciences
Pojeong Park, Heather Kang, Thomas M. Sanderson, Zuner A. Bortolotto, John Georgiou, Min Zhuo, Bong-Kiun Kaang, Graham L. Collingridge
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2019)