Article
Biochemistry & Molecular Biology
Shohei Sakai, Seiji Watanabe, Okiru Komine, Akira Sobue, Koji Yamanaka
Summary: The study found that MAM disruption is a common pathological feature in ALS, but there is currently a lack of simple and effective tools for monitoring the status of MAM. Scientists have developed two new MAM reporter genes that can monitor the effects of various ALS-causative genes on MAM.
Article
Biochemistry & Molecular Biology
Hyun Ku Yeo, Tae Hyun Park, Hee Yeon Kim, Hyonchol Jang, Jueun Lee, Geum-Sook Hwang, Seong Eon Ryu, Si Hoon Park, Hyun Kyu Song, Hyun Seung Ban, Hye-Jin Yoon, Byung Il Lee
Summary: The study revealed the functions of PTPIP51 in phospholipid binding/transfer, particularly of phosphatidic acid, in cells. Depletion of PTPIP51 reduces the mitochondrial cardiolipin level, and the interaction between PTPIP51 and VAPB is mediated by specific structural motifs.
Article
Food Science & Technology
Sicong Tian, Peng Lei, Jing Zhang, Yao Sun, Baolong Li, Yujuan Shan
Summary: The study revealed that SFN balances Ca2+ homeostasis in the MAM by regulating Ca2+ flux through the Ca2+ channels IP3R and MCU. SFN widens the distance between ER and mitochondria, down-regulates MAM tether protein mitofusin-2, alleviates Ca2+ overload in mitochondria, increases mitochondrial DNA quantities and membrane potential, inhibits ROS production, and enhances mitochondria complexes IV content and ATP synthesis.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Environmental Sciences
Lepeng Gao, Chang Zhang, Sicong Yu, Shuang Liu, Guoxia Wang, Hainan Lan, Xin Zheng, Suo Li
Summary: This study investigated the effects of mono-n-butyl phthalate (MBP) on the in vitro maturation (IVM) and development of porcine oocytes using SMART-seq technology. The results showed that differentially expressed genes were enriched in cytoskeleton, cell apoptosis, endoplasmic reticulum (ER), and mitochondria. Glycine (Gly) was found to improve oocyte developmental potential by regulating mitochondrial and ER function. Gly supplementation significantly reduced MBP-induced spindle abnormalities and mitochondria-associated ER membrane (MAM) interactions. Moreover, Gly treatment restored the distribution of certain proteins and genes, leading to decreased intracellular free calcium concentration, increased ER levels and mitochondrial membrane potential, reduced levels of reactive oxygen species, and increased levels of Glutathione.
ENVIRONMENTAL POLLUTION
(2022)
Article
Cell Biology
Benjamin Gottschalk, Zhanat Koshenov, Olaf A. Bachkoenig, Rene Rost, Roland Malli, Wolfgang F. Graier
Summary: Endoplasmic reticulum (ER) critically depends on ATP supply for its functions. The disruption of protein trafficking and folding in the ER leads to ER stress and the unfolded protein response (UPR). During ER stress, the stability and lifetime of mitochondrial associated ER membranes (MAM) increase, resulting in an increase in mitochondrial activity and ATP generation, which in turn enhances the ATP supply for the ER. The increased stability and lifetime of MAMs during ER stress rely on the mitochondrial fusion protein Mitofusin2 (MFN2). Knockdown of MFN2 impairs the response of mitochondria to ER stress and reduces ATP supply for the ER.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Robert Gilkerson, Patrick De la Torre, Shaynah St. Vallier
Summary: Mammalian mitochondria play a crucial role in cellular stress response and development outcomes, responding to stimuli and stresses through structural dynamics. Fusion and fission of mitochondria, regulated by OPA1, are essential for maintaining organelle structure and function. The proteolytic mechanism involving OMA1 provides sensitive regulation of mitochondrial structure/function and is implicated in apoptotic pathways and cellular stress response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anna Fernandez Bernal, Natalia Mota, Reinald Pamplona, Estela Area-Gomez, Manuel Portero-Otin
Summary: Amyotrophic lateral sclerosis (ALS) is characterized by the death of motor neurons (MNs), and dysfunction in mitochondrial-associated membranes (MAMs) may play a crucial role in the cellular disarrangements in ALS, including dysregulations in lipid metabolism and calcium homeostasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Shuvadeep Maity, Pragya Komal, Vaishali Kumar, Anshika Saxena, Ayesha Tungekar, Vaani Chandrasekar
Summary: Accumulation of misfolded proteins is common in neurodegenerative diseases, particularly those caused by abnormal PolyQ expansions. The disruption of protein homeostasis in the endoplasmic reticulum (ER) and activation of the unfolded protein response (UPR) pathway are linked to the development of Huntington's disease (HD). Furthermore, proteins at ER-mitochondria contact sites (MAMs) play a significant role in HD progression. This review provides updated information on UPR pathways and their involvement in HD, as well as the role of MAMs in disease progression. Therapeutic interventions focusing on ER stress response proteins and ER-mitochondrial contact proteins are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Lisenn Lalier, Vincent Mignard, Marie-Pierre Joalland, Didier Lanoe, Pierre-Francois Cartron, Stephen Manon, Francois M. Vallette
Summary: This study revealed that Bcl2 is mainly localized in the ER, and is translocated to MAM and mitochondria during apoptosis, with its interaction with TOM20 playing a significant role. The findings suggest that the Bcl2-TOM20 interaction is proapoptotic and contributes to the control of apoptosis.
CELL DEATH & DISEASE
(2021)
Article
Environmental Sciences
Xiaoyu Wang, Huabin Cao, Yukun Fang, He Bai, Jing Chen, Chenghong Xing, Yu Zhuang, Xiaoquan Guo, Guoliang Hu, Fan Yang
Summary: This study revealed the interaction between Mfn2-dependent MAM dynamics and autophagy in duck renal tubular epithelial cells under Cu exposure. Excessive Cu disrupted MAM integrity and activated autophagy.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemistry & Molecular Biology
Qian Wang, Wenying Chen, Boyang Zhang, Zilu Gao, Qipeng Zhang, Huiqiong Deng, Lingyun Han, Xiao Li Shen
Summary: PFOA induces mitochondrial-mediated apoptosis in liver cells, which is regulated by communication between the endoplasmic reticulum and mitochondria.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Sophie Moulin, Amandine Thomas, Stefan Wagner, Michael Arzt, Herve Dubouchaud, Frederic Lamarche, Sophie Bouyon, Guillaume Vial, Diane Godin-Ribuot, Jean-Louis Pepin, Claire Arnaud, Elise Belaidi
Summary: This study found that IH-induced ER stress, MAM alterations, and mitochondrial dysfunction were mediated by HIF-1, ultimately leading to cardiomyocyte apoptosis. This suggests that modulation of HIF-1 may help mitigate the adverse effects of sleep-disordered breathing on the heart.
Article
Biology
Maria Sol Herrera-Cruz, Megan C. Yap, Nasser Tahbaz, Keelie Phillips, Laurel Thomas, Gary Thomas, Thomas Simmen
Summary: Rab32 promotes degradation of mitochondrial-proximal ER membranes via autophagy with the help of its effector RTN3L, a process proposed to be termed MAM-phagy.
Article
Biochemistry & Molecular Biology
Simon Couly, Yuko Yasui, Tsung-Ping Su
Summary: SIGMAR1 is a unique receptor chaperone protein that binds various ligands and interacts with other proteins to affect cellular functions. It confers protection against neurodegeneration in the nervous system, making it a promising therapeutic target for multiple diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jing Li, Fangzheng Qi, Huishan Su, Chuanshan Zhang, Qing Zhang, Ying Chen, Ping Chen, Linjia Su, Yanan Chen, Yuqi Yang, Zhesheng Chen, Sihe Zhang
Summary: GRP75-faciliated MAM formation is enriched in cisplatin-resistant ovarian cancer cells. Diminishing MAM integrity by GRP75-deficiency reduces ER-to-mitochondrial Ca2+ transfer, accelerates mitochondrial dysfunction and ROS accumulation, and enhances cisplatin-triggered apoptotic cell death.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
