p-Aminophenol-induced cytotoxicity in Jurkat T Cells: Protective effect of 2(RS)-n-propylthiazolidine-4(R)-carboxylic acid

Acetaminophen (APAP) is hepatotoxic and can cause toxicity in Jurkat T cells. p-Aminophenol (PAP), an industrial chemical and APAP metabolite, is nephrotoxic and hepatotoxic. Its potential toxicity in JuArkat T cells was investigated. PAP (10250 mu M) caused toxicity (decreased survival and increased LDH activity in incubation medium) and GSH depletion. At a concentration of 100 mu M but not 250 mu M, PAP increased DNA fragmentation. It decreased p-Akt levels (Elisa) and at higher concentrations decreased p-Akt expression (Western blotting). It had no effect on FasL expression. The cysteine precursor 2(RS)-n-propylthiazolidine-4(R)-carboxylic acid (250 mu M) attenuated the PAP (100 mu M)-induced decrease in viability and prevented GSH depletion and increased DNA fragmentation. It attenuated the PAP-induced decrease in p-Akt levels and protected against the decrease in p-Akt expression. The results demonstrate PAP-induced toxicity and suggest that it is due at least in part to apoptosis and involves GSH depletion and p-Akt inactivation. (c) 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:7178 2012; View this article online at . DOI 10.1002/jbt.20402