☆ 4.5 Article

Manganese Accumulation in the Mouse Ear Following Systemic Exposure

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2008)

Journal

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY

Volume 22, Issue 5, Pages 305-310

Publisher

WILEY

DOI: 10.1002/jbt.20241

Keywords

Manganese; Mouse; Ear; ZIP8; Slc39a8; Slc39a14; Dmt1

Categories

Biochemistry & Molecular Biology Toxicology

Funding

NIEHS NIH HHS [P30 ES006096, ES10416, P30 ES006096-159007, R01 ES010416, R01 ES010416-09, ES06096] Funding Source: Medline
NIOSH CDC HHS [T42/OH008432-01, T42 OH008432] Funding Source: Medline
NATIONAL INSTITUTE FOR OCCUPATIONAL SAFETY AND HEALTH [T42OH008432] Funding Source: NIH RePORTER
NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [R01ES010416, P30ES006096] Funding Source: NIH RePORTER

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Abstract

There is evidence in human populations that exposure to manganese (Mn), or Mn in combination with excessive noise exposure, results in hearing loss. Quantitative reverse-transcriptase polymerase chain reaction revealed expression of the metal transporters DMT1, ZIP8, and ZIP14 in control mouse ears. ZIP8 is known to have a high affinity (K-m = 2.2 mu M) for Mn transport, and ZIP8 protein was localized to the blood vessels of the ear by immunohistochemistry. We treated mice (strains C57BL/6J and DBA/2J) with Mn (100 mg/kg MnCl2, by subcutaneous injection, on three alternating days), and Mn was significantly elevated in the ears of the treated mice. Mn concentrations remained elevated over controls for at least 2 weeks after treatment. These studies demonstrate that metal transporters are present in the mouse ear and that Mn can accumulate in the ear following systemic exposure. Future studies should focus on whether Mn exposure is associated with hearing deficits. (C) 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:305-310, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10:1002/jbt.20241

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