Journal
JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
Volume 28, Issue 6, Pages 605-620Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/0883911513506665
Keywords
Directional cell migration; poly(ethylene glycol) brushes; surface gradient; gradient biomaterials; gradient patterns; vascular smooth muscle cells
Funding
- Natural Science Foundation of China [20934003, 51003094]
- National Basic Research Program of China [2011CB606203]
- Young Teacher Programs Foundation of Ministry of Education of China [20100101120034]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars from State Education Ministry, Qianjiang outstanding researcher funding of Zhejiang Province [J20110541]
- Research Project of Department of Education of Zhejiang Province [Z201018687]
Ask authors/readers for more resources
Directional migration of cells in vitro can mimic corresponding biological events in vivo, which provides a way to determine the cascade responses in the tissue regeneration process and develop novel criteria for the design of tissue-inductive biomaterials. In this work, a density gradient of methoxy poly(ethylene glycol) brushes (from 0.37 to 0.95 chains/nm(2)) on plain and striped pattern surfaces was fabricated, using a dynamically controlled reaction process, and characterized by X-ray photoelectron spectroscopy and quartz crystal microbalance with dissipation. Adhesion and migration behavior of vascular smooth muscle cells were studied on the gradient and gradient-patterned surfaces. The vascular smooth muscle cells exhibited preferential orientation and enhanced directional migration on the gradient surface toward the lower end of the methoxy poly(ethylene glycol) density. By introducing methoxy poly(ethylene glycol) chemical striped patterns in parallel with the gradient direction on the surface, the extent of cell orientation and directional migration were significantly improved. Due to the synergetic effects of surface methoxy poly(ethylene glycol) striped patterns and gradient cues, almost all cells were oriented, and 67% of the cells were observed to move unidirectionally.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available