Chitosan/Alginate Multilayer Scaffold Encapsulating Bone Marrow Stromal Cells In Situ on Titanium

A biofilm-like scaffold with bone marrow stromal cells (BMSC) encapsulated in situ was constructed on a titanium surface using a layer-by-layer self-assembly technique for the potential application for dental or joint implant. The scaffold was formed by depositing a single layer of positively charged poly(L-lysine) on a negatively charged NaOH-treated titanium substrate, followed by alternate immersion into a negatively charged alginate-BMSC suspension and positively charged chitosan solution, respectively, and terminated a layer of chitosan. The cell-encapsulated scaffolds were evaluated by scanning electron microscopy and confocal laser scanning microscopy. The BMSC remained viable and grew well in the scaffold. This approach provides a method for the preparation of tissue engineering scaffold on titanium surfaces.