Article
Multidisciplinary Sciences
Nosayba Al-Azzam, Anas Alazzam
Summary: The wettability of a polymer surface is crucial for cell-cell interaction and behavior. This article investigated two techniques, oxygen plasma treatment and graphene oxide coating, to pattern the surface wettability of a COC substrate in order to create hydrophilic patterned regions on a hydrophobic surface. Comparing the two techniques, GO coating resulted in a more stable wettability and increased roughness of the patterned area. Both techniques effectively modulated cell adhesion and achieved micropatterning of cell growth.
Article
Chemistry, Multidisciplinary
Kun Wang, Hyang Seol, Xuan Liu, Huifeng Wang, Gang Cheng, Sangil Kim
Summary: This paper introduces a portable, low-cost plasma separation device that can rapidly extract plasma in a simple three-step operation, improving the efficiency of plasma extraction. By using a special membrane material, the device can effectively inhibit surface fouling of blood cells and membrane flux, ensuring the quality of extracted plasma and reducing operation time.
Article
Chemistry, Multidisciplinary
Tosin Ogunlusi, Jeremy D. Driskell
Summary: Proteins adsorbed to gold nanoparticles (AuNPs) are crucial for various biomedical applications. Understanding the interaction between the protein and AuNP is essential for the bioconjugate to function effectively.
Article
Engineering, Biomedical
A. D. Abreu-Rejon, W. Herrera-Kao, A. May-Pat, A. Avila-Ortega, N. Rodriguez-Fuentes, J. A. Uribe-Calderon, J. M. Cervantes-Uc
Summary: Surface modification of Tecoflex SG-80A Polyurethane films using polyethylene glycol (PEG) chains resulted in improved surface properties. The PEG-grafted polyurethane extracts showed increased viability of osteoblasts but had no significant effect on fibroblasts. Lower PEG grafting densities promoted healthier cell morphology.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2022)
Article
Nanoscience & Nanotechnology
Yi Zhang, Ahmed Khalil Khan, Deanna See, Jackie Y. Ying
Summary: Paper-based platforms are beneficial for monitoring infectious diseases in low-resource settings due to their simplicity and low cost. Nitrocellulose and cellulose are commonly used materials in these platforms. Examining the microstructure of cellulose paper, it was found that the cellulose fibers are covered by thin films, which are inert to protein adsorption. By selectively removing these inert films, protein adsorption to cellulose paper was enhanced and the performance of lateral flow assays was improved. This suggests that cellulose paper could potentially replace nitrocellulose as a cheaper substrate for rapid test kits.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Environmental
Pengxu Wang, Qing Liu, Wenliang Fu, Chao Zhang, Guiling Cai, Weiwei Xing, Hongliang Dai, Hongya Geng, Donggang Xu
Summary: Marine mussels have evolved specialized proteins to effectively adhere to various surfaces. However, studying the mechanisms of adhesion and cohesion has been challenging due to the lack of materials and methods. This study analyzed the adhesive and cohesive properties of specific protein domains using force spectroscopy. The results revealed different adhesion forces for different pair structures and identified cation-pi interactions as a strong cohesive force. This study provides insights into peptide adhesion and design principles for artificial adhesive layers.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Physics, Applied
Anastasiya O. Solovieva, Elizaveta S. Permyakova, Konstantin Ershov, Ksenia Bakhareva, Svetlana M. Miroshnichenko, Philipp Kiryukhantsev-Korneev, Anton S. Konopatsky, Josef Polcak, Dmitry Shtansky, Anton M. Manakhov
Summary: COOH plasma polymer layers deposited onto polycaprolactone (PCL) nanofibers can accelerate diabetic wound healing, showing promising clinical applications.
PLASMA PROCESSES AND POLYMERS
(2022)
Article
Hematology
E. O. Artemenko, S. I. Obydennyi, K. S. Troyanova, G. A. Novichkova, D. Y. Nechipurenko, M. A. Panteleev
Summary: In this study, we investigated the adhesive properties of circulating plasma-derived and platelet-derived microvesicles (MVs) and their potential role in thrombus growth. The results showed that both types of MVs weakly bound fibrinogen but did not stably adhere to immobilized fibrinogen. Moreover, the presence of these MVs did not significantly affect thrombus formation. Therefore, MVs present in the plasma of healthy individuals are unlikely to directly contribute to thrombus formation under arterial flow conditions.
THROMBOSIS RESEARCH
(2024)
Article
Biochemical Research Methods
Markus Mozgovicz, Andreas Fischer, Cecile Brocard, Alois Jungbauer, Nico Lingg
Summary: Immobilized metal affinity chromatography (IMAC) is a powerful technique for capturing and purifying biopharmaceuticals. Protein recovery can be compromised due to spreading and unfolding of the protein, leading to fouling of the stationary phase. This study investigates the kinetics of irreversible adsorption of a protease on an IMAC resin and suggests that L-arginine sulfate can decelerate the irreversible adsorption.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Engineering, Biomedical
Rashed Almousa, Dong Xie, Yong Chen, Jiliang Li, Gregory G. Anderson
Summary: This study aims to covalently coat negatively charged polymer brushes onto the surface of TPU to reduce contact angle, protein adsorption, and cell/bacterial adhesion. Results show that these modified surfaces exhibit significantly reduced values in all tests, with negatively charged polymers performing the best.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Mineo Hashizume, Michihisa Hirashima
Summary: This study investigates the immobilization of poly(4-vinylphenol) (PVP) on polyimide (PI) film surfaces through hot pressing. The results show that increasing hot-press temperature leads to a higher amount of PVP immobilization. The mechanism of immobilization is discussed in relation to the physical properties of the PI film surfaces.
Article
Chemistry, Multidisciplinary
Hyun Jeong Jeon, Ara Jung, Hee Jin Kim, Jeong San Seo, Jun Young Kim, Moon Seop Yum, Bomi Gweon, Youbong Lim
Summary: This study proposes a vacuum plasma device for surface treatment of dental implants, showing significant improvements in protein adsorption, cell adhesion, and proliferation efficiency through plasma treatment. The device has the potential to enhance osseointegration efficiency.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Jingxia Wang, Chaorong Peng, Xijing Yang, Maojun Ni, Xiaobin Zhang, Zhenqiang Shi, Hao Chen, Siyang Liu, Lunqiang Jin, Changsheng Zhao
Summary: In this study, a new approach using a polyethersulfone (PES) membrane grafted with polyethylene glycol methyl acrylate (PEG-MA) was proposed to remove endotoxins. The PES-g-PEG membrane was prepared by phase inversion method after the PES polymer was grafted with PEG-MA via γ irradiation. Polydopamine (PDA) was then coated onto the PES-g-PEG membrane, and lysozyme (Lyz) was immobilized with PDA through a Schiff base reaction. The PES-g-PEG/PDA/Lyz membrane showed high adsorption capacity for endotoxins and has potential application in the treatment of endotoxemia.
Article
Multidisciplinary Sciences
Jianying Zhang, Feng Li, Tyler Augi, Kelly M. Williamson, Kentaro Onishi, MaCalus Hogan, Matthew D. Neal, James H-C Wang
Summary: Platelet-rich plasma (PRP) containing PLT HMGB1 plays a crucial role in tendon wound healing by reducing inflammation, increasing local HMGB1 levels, and recruiting stem cells to the injured area. The efficacy of PRP treatment for tendon injuries in clinics may be dependent on the presence of PLT HMGB1 in PRP preparations.
Article
Chemistry, Multidisciplinary
Francesco Perricelli, Massimo Boscaglia, Massimiliano Cantiano, Luca Spitaleri, Maria Elena Fragala, Antonino Gulino
Summary: In this study, the morphological and chemical modifications of fluorinated photodefinable polybenzoxoles (F-PBOs) induced by Ar plasma treatments were investigated using atomic force microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. This process, which aims to remove surface contaminants and increase surface roughness for better adhesion, is crucial in the manufacturing of microelectronic devices. The study found that Ar plasma treatments significantly increase the polymer surface roughness but also lead to the formation of unwanted silver fluoride species when a patterned silver layer is present on F-PBO.
Article
Biochemistry & Molecular Biology
Tugba Dursun Usal, Metin Yesiltepe, Deniz Yucel, Yildirim Sara, Vasif Hasirci
Summary: In this study, a 3D-printed PCL nerve guide using an aligned gelatin-poly(3-hydroxybutyrate-co-3-hydroxyvalerate) electrospun mat was produced, showing significant potential for assisting the regeneration of injured peripheral nerves. Functional recovery and improved nerve conduction velocities were observed in animal experiments using the nerve guides, with the highest new tissue organization observed when the guides contained the electrospun mat and seeded cells.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Chemistry, Analytical
Menekse Ermis, Ezgi Antmen, Ozgur Kuren, Utkan Demirci, Vasif Hasirci
Summary: Microfabrication technologies have been widely used in cell biology and cancer research. In this study, a transparent cell culture chip was developed to monitor the behavior of breast cancer cells. The chip proved to be advantageous in analyzing live cells and studying breast cancer cell-substrate interactions.
Article
Engineering, Biomedical
Deniz Atila, Vasif Hasirci, Aysen Tezcaner
Summary: This study optimized the electrospinning process of core/shell poly(methyl methacrylate)/silk fibroin fibers to mimic the extracellular matrix and induce tissue regeneration. By adjusting the morphology and chemical properties of the fibers, the water contact angle and porosity of the mats could be altered. The core/shell fibers exhibited intermediate mechanical properties, porous structure, and fine morphology. These fibers promoted the attachment and proliferation of dental pulp stem cells.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Surgery
Enrico Lauro, Ilaria Corridori, Lorenzo Luciani, Alberto Di Leo, Alberto Sartori, Jacopo Andreuccetti, Diletta Trojan, Giovanni Scudo, Antonella Motta, Nicola M. Pugno
Summary: This study investigated the biomechanics of stapled sutures compared to handsewn sutures for midline reconstruction. The results showed that stapled sutures had higher strength but lower deformability and rigidity compared to handsewn sutures. Oversewing a stapled suture improved its efficiency, especially for larger defects. Hybrid sutures appeared to be a good compromise.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Article
Chemistry, Medicinal
Syafira Masri, Faraheda Amilia Mohd Fauzi, Sarah Batrisyia Hasnizam, Aizzaty Sulha Azhari, Juliana Edora Amin Lim, Looi Qi Hao, Manira Maarof, Antonella Motta, Mh Busra Fauzi
Summary: Three-dimensional (3D) in vitro skin models are commonly used in cosmetic and pharmaceutical research to reduce the need for animal testing. The use of three-dimensional (3D) bioprinting allows for the fabrication of cell-based bioinks, improving the ability to rapidly manufacture these models while maintaining important properties. This study found that a gelatin-polyvinyl alcohol (GPVA) bioink, crosslinked with genipin (GNP), and cultured with human dermal fibroblasts (HDFs), demonstrated excellent biocompatibility and potential for rapid manufacturing of a single dermal layer.
Review
Orthopedics
Andreas S. Croft, Eugenia Spessot, Promita Bhattacharjee, Yuejiao Yang, Antonella Motta, Michael Woltje, Benjamin Gantenbein
Summary: Silk is a promising biomaterial for repairing intervertebral discs and has shown positive results in preclinical studies. It has been extensively studied for repairing different parts of intervertebral discs to address chronic low back pain.
Article
Materials Science, Ceramics
Senem Buyuksungur, Pinar Yilgor Huri, Juergen Schmidt, Iulian Pana, Mihaela Dinu, Catalin Vitelaru, Adrian E. Kiss, Dilara Goksu Tamay, Vasif Hasirci, Alina Vladescu, Nesrin Hasirci
Summary: Ti6Al4V alloy has been widely used as implant material in dental and orthopedic surgeries for its superior compatibility, low density, and corrosion resistance compared to other metals. Surface modification of the alloy is necessary to improve material-tissue interaction and bone integration. In this study, Ti6Al4V alloy surfaces were modified by RF magnetron sputtering and coated with zinc-doped hydroxyapatite. The resulting coating exhibited high stability, crystalline structure, enhanced corrosion resistance, osteointegration, and antimicrobial effectiveness against Escherichia coli (E. coli) bacteria.
CERAMICS INTERNATIONAL
(2023)
Article
Biophysics
Deniz Atila, Dilek Keskin, Yuan-Ling Lee, Feng-Huei Lin, Vasif Hasirci, Aysen Tezcaner
Summary: Injectable hydrogels have potential in dental pulp regeneration due to their ability to fill non-uniform voids. In this study, gelatin methacrylate/thiolated pectin hydrogels carrying electrospun core/shell fibers were designed for vital pulp regeneration. The release of melatonin and Tideglusib from the fibers induced stem cell proliferation and odontoblastic differentiation. This hydrogel system holds promise for pulp tissue regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biophysics
Juergen Schmidt, Iulian Pana, Anna Bystrova, Mihaela Dinu, Yuri Dekhtyar, Catalin Vitelaru, Marks Gorohovs, Ioana Maria Marinescu, Pinar Yilgor Huri, Dilara Goksu Tamay, Gabriela Andreea Juravlea, Senem Buyuksungur, Anca Constantina Parau, Vasif Hasirci, Nesrin Hasirci, Alina Vladescu
Summary: The purpose of this study was to investigate the effect of Zn doped CaP coatings prepared by micro-arc oxidation method on MgCa1 alloy degradation. The results showed that the addition of Zn into CaP increased the corrosion resistance of MgCa1 alloy and enhanced the adhesion strength of the coatings. All samples exhibited cytocompatibility and the degradation rate of MgCa1 alloy in simulated body fluids (SBF) or DMEM was decreased by coating with CaP, which was further reduced by adding a small amount of Zn. The samples with CaP coatings and Zn doped CaP coating also demonstrated antibacterial efficacy against E.coli.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Materials Science, Biomaterials
Ezgi Antmen, Menekse Ermis, Ozgur Kuren, Kemal Beksac, Cigdem Irkkan, Vasif Hasirci
Summary: In this study, the combination of conventional molecular-level evaluations and in vitro methods on micropatterned biomaterial surfaces was used to assess breast adenocarcinoma patients. The results showed that molecular markers and shape descriptors could distinguish cells from different locations and patients. Cells with the highest metastatic and invasive properties exhibited both the highest deformability and the highest level of metastatic markers. By combining molecular markers with nuclear deformation, the detection and separation of subpopulations in heterogeneous breast cancer specimens at the single-cell level can be improved.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Physical
Ibrahim N. N. Amirrah, Izzat Zulkiflee, M. F. Mohd Razip Wee, Asad Masood, Kim S. S. Siow, Antonella Motta, Mh Busra Fauzi
Summary: This study aimed to develop an acellular, ready-to-use collagen bioscaffold with an antibacterial coating for the immediate treatment of skin wounds and to prevent infection. The crosslinker genipin demonstrated better physicochemical and mechanical properties, as well as enhanced biodegradability and biocompatibility compared to the non-crosslinked control. Similarly, the plasma-polymerised carvone coating on the crosslinked collagen scaffold showed promising results in terms of antibacterial properties and support for human skin cell proliferation and viability.
Article
Materials Science, Biomaterials
Carlos Mota, Devid Maniglio, Francesca Perin, Eugenia Spessot, Anna Fama, Alessio Bucciarelli, Emanuela Callone, Antonella Motta
Summary: Extrusion-based bioprinting is a widely used technology due to its affordability and wide range of processable materials. However, the formulation of new inks for this technique is time-consuming. This study developed a dynamic printability window model to assess the printability of polysaccharide blend inks and successfully verified its predictive capability on an untested blend.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Nanoscience & Nanotechnology
Duygu Ege, Vasif Hasirci
Summary: osteochondral tissue regeneration is difficult due to its complex organization. 3D printing, specifically using extrusion, stereolithography, and inkjet printing approaches, is employed to design complex multilayer structures. Different materials and methods are discussed, along with the potential for improving material properties and the future prospects of 3D printing in osteochondral tissue repair.
ACS APPLIED BIO MATERIALS
(2023)
Article
Chemistry, Physical
Yu Zhao, Hao Lu, Daizong Qi, Antonella Motta, Janine Froehlich-Nowoisky, Jing Chen, Yuling Sun, Mischa Bonn
Summary: This study explores the ice recrystallization inhibition (IRI) activity of silk proteins from Bombyx mori and finds that silk fibroin (SF) has higher IRI activity than silk sericin (SS). SF aqueous solutions perform better in inhibiting ice recrystallization than SF phosphate-buffered saline solutions. This work is significant for broadening the applications of silk proteins in biomedical fields.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Polymer Science
Devid Maniglio, Elia Bissoli, Emanuela Callone, Sandra Dire, Antonella Motta
Summary: Damages to the intervertebral disc (IVD) due to improper loading or degeneration result in back pain. Hydrogels, with their ability to simulate soft tissues and tunable properties, are attractive materials for IVD remediation. In this study, hydrogels were produced through the crosslinking of different mixtures of polymers, allowing for the control of both shear properties and hydrophilicity. NMR and DSC were used to investigate the structure and properties of the hydrogels.