Article
Microbiology
Snaedis H. Bjornsdottir, Solveig K. Petursdottir, Elisabet E. Gudmundsdottir, Edda Olgudottir, Sigmar K. Stefansson, Tara Robertsdottir, Asbjorn H. Sigurpalsson, Solveig K. Olafsdottir, Olafur H. Fridjonsson, Gudmundur O. Hreggvidsson
Summary: Red-pigmented non-sporeforming bacteria were isolated from intertidal hot springs in NW-Iceland, and identified as a novel species named Rhodocaloribacter litoris gen. nov., sp. nov. based on morphological, growth conditions and molecular characteristics.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Microbiology
Christaline George, Chloe Xue Qi Lim, Yan Tong, Stephen Brian Pointing
Summary: The Sembawang Hot Spring in Singapore is located at the foot of a geological feature called the Bentong-Raub Suture Zone. The hot spring exhibits a poly-extreme environment and supports distinct microbial communities along an environmental gradient. The high abundance of phototrophic bacteria and the presence of chemoautotrophs and heterotrophs reflect the known thermal ranges and allochthonous leaf input in the hot spring. The findings contribute to the understanding of hot spring microbiomes and address a biogeographic knowledge gap for the region.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Ilona A. Ruhl, Andriy Sheremet, Chantel C. Furgason, Susanne Krause, Robert M. Bowers, Jessica K. Jarett, Triet M. Tran, Stephen E. Grasby, Tanja Woyke, Peter F. Dunfield
Summary: GAL08 is a bacteria belonging to the Acidobacteria phylum, and it was found in abundance in hot spring sediment. Through genomic analysis, it was discovered that GAL08 has heterotrophic metabolism and a microaerophilic lifestyle.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Saumashish Mukherjee, Tushar Dilipchand Lodha, Jogi Madhuprakash
Summary: This study describes the xylan degradation potential and enzyme machinery of the Paenibacillus sp. LS1 strain, which is able to efficiently degrade xylan polymers from hardwood and graminaceous plants. The strain possesses an extensive repertoire of xylanolytic enzymes and is capable of utilizing xylan as the sole carbon source for growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Freddy Bunbury, Carlos Rivas, Victoria Calatrava, Amanda N. Shelton, Arthur Grossman, Devaki Bhaya
Summary: Phototactic strategies may be adaptable and finely tuned to the light and local environment. Differences in phototactic responses and photoreceptor genes were observed between Synechococcus isolates from thermal springs in Yellowstone National Park. These findings highlight the diversity of phototactic strategies even among closely related cyanobacteria and provide insights into the importance of phototaxis in microbial biofilm communities.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Muhammad Yasir, Arooj K. Qureshi, Esam Azhar
Summary: This study analyzed sediment samples from the non-volcanic Tatta Pani hot springs in Azad Kashmir using both culture-dependent and culture-independent techniques. The results showed high microbial diversity in the enriched sediment samples but adverse changes in the composition of relatively dominant bacteria. Metagenomic analyses are recommended for further study of hot spring microbiota diversity and functionality.
Article
Microbiology
Shigeru Kawai, Mikako Ishikawa, Satoshi Hanada, Shin Haruta
Summary: A novel thermophilic chemotrophic bacterium, Hydrogenophilus thiooxidans sp. nov., was isolated from Nakabusa Hot Spring in Japan. The bacterium can grow both chemoheterotrophically and autotrophically under different conditions.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Mohit Kumar Saini, Shohei Yoshida, Aswathy Sebastian, Eri Hara, Hideyuki Tamaki, Nathan T. Soulier, Istvan Albert, Satoshi Hanada, Marcus Tank, Donald A. Bryant
Summary: A new microbial strain, named Elioraea tepida, was isolated from an alkaline siliceous hot spring in Yellowstone National Park, USA. The strain grows chemoheterotrophically by oxygen-dependent respiration and can also undergo photoheterotrophic growth under strictly oxic conditions. It does not exhibit photoautotrophic growth under oxic or anoxic conditions, indicating that it belongs to an aerobic anoxygenic phototrophic bacterium.
Article
Microbiology
Juan Wang, Ming Deng, En-Min Zhou, Lei Ran, Cui-Ping Miao, Yi-Qing Li, Jun-Mei Ding, Shu-Kun Tang
Summary: The bacterial strain YIM B00624(T) isolated from a hot spring in Tengchong, Yunnan province, China, belongs to the genus Paenibacillus. It exhibits specific characteristics such as growth on specific medium, major fatty acids, diagnostic amino acid, and major polar lipids. The strain represents a novel species within the genus based on physiological, phenotypic, and chemotaxonomic data, thus proposed to be named Paenibacillus hamazuiensis.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Microbiology
Kentaro Miyazaki, Natsuki Tomariguchi, Yuko Ueno
Summary: The four Thermus thermophilus strains isolated from Arima Hot Spring in Japan showed high nucleotide identity to each other and those from the same spot, but lower identity to strains from geographically different spots, indicating habitat-specific genomic conservation.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Engineering, Marine
Vincenzo Zammuto, Antonio Spano, Marco Sebastiano Nicolo, Emanuela Grillo, Maria Teresa Caccamo, Salvatore Magazu, Simone Cappello, Concetta Gugliandolo
Summary: The exploitation of thermophilic hydrocarbon-utilizing bacilli could provide novel environmentally friendly surfactants. In this study, 80 thermophilic bacilli were isolated from shallow hydrothermal vents and 15 strains were found to be able to utilize hydrocarbons and produce biosurfactants. Two of the selected strains produced surfactants with emulsifying activity and the ability to remove different hydrocarbons and vegetable oils.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Soufiane Maski, Serigne Inssa Ngom, Bahia Rached, Taha Chouati, Mohamed Benabdelkhalek, Elmostafa El Fahime, Mohamed Amar, Christel Bera-Maillet
Summary: Thermophilic bacterium Bacillus aerius from hot springs in Morocco shows potential for utilizing hemicellulosic biomass and possesses a wide range of hydrolytic capabilities. Reclassified as Bacillus paralicheniformis through whole-genome sequencing, it offers valuable insights into carbohydrate metabolism mechanisms.Annotation of its genomic sequences reveals a variety of genes encoding enzymes involved in plant cell wall carbohydrate degradation, highlighting its relevance for biotechnological applications.
Article
Chemistry, Applied
Xiheng Kang, Yun-Yan Wang, Shuangfei Wang, Xueping Song
Summary: The study focused on analyzing the hydrothermal degradation products of xylan and xylose, finding that furfural and formic acid reached maximum values under different conditions. Adjusting the initial pH of the xylan solution effectively reduced furfural concentration, with formic acid formation preferred under alkaline conditions. A new hydrothermal degradation pathway of xylan in alkaline solution was proposed, aiming to improve biofuel production efficiency through a deeper understanding of xylan degradation during hot water pre-treatment.
CARBOHYDRATE POLYMERS
(2021)
Article
Environmental Sciences
Yueling Sun, Ying Zhang, Xiaohan Hao, Xiaohan Zhang, Yongzheng Ma, Zhiguang Niu
Summary: A novel marine bacterium, Exiguobacterium marinum a-1, was successfully isolated from the 'plastisphere' and shown to be capable of utilizing additive-free polypropylene films as its primary energy and carbon source. This study highlights the potential of microorganisms in the 'plastisphere' to metabolize plastic polymers and emphasizes the intrinsic importance of the new strain a-1 in mitigating plastic pollution.
ENVIRONMENTAL POLLUTION
(2023)
Article
Engineering, Chemical
Yuanyuan Zhang, Kunling Huang, Xuan Chen, Min Wei, Xiaopeng Yu, Hongfei Su, Pin Gan, Kefu Yu
Summary: This study investigated the inactivation of ciliate Uronema marinum (U. marinum) as a surrogate for protozoa by the UV/peroxydisulfate (PDS) system. The addition of 1 mM PDS significantly enhanced the inactivation of U. marinum by UV exposure. The reactive species responsible for the inactivation were identified as SO4- and OH. This research suggests that upgrading UV disinfection to the SO4- based advanced oxidation technology will be beneficial for inactivating protozoa in marine water.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lucas Auer, Mahendra Mariadassou, Michael O'Donohue, Christophe Klopp, Guillermina Hernandez-Raquet
MOLECULAR ECOLOGY RESOURCES
(2017)
Article
Microbiology
Lucas Auer, Adele Lazuka, David Sillam-Dusses, Edouard Miambi, Michael O'Donohue, Guillermina Hernandez-Raquet
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Zhong-Peng Guo, Julien Robin, Sophie Duquesne, Michael Joseph O'Donohue, Alain Marty, Florence Bordes
BIOTECHNOLOGY FOR BIOFUELS
(2018)
Article
Food Science & Technology
Carole Camarasa, Hubert Chiron, Fayza Daboussi, Guy Della Valle, Claire Dumas, Vincent Farines, Juliane Floury, Valerie Gagnaire, Nathalie Gorret, Joelle Leonil, Jean-Roch Mouret, Michael J. O'Donohue, Jean-Marie Sablayrolles, Jean-Michel Salmon, Luc Saulnier, Gilles Truan
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2018)
Article
Food Science & Technology
Hugo de Vries, Monique A. V. Axelos, Pascale Sarni-Manchado, Michael O'Donohue
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2018)
Article
Biotechnology & Applied Microbiology
Adele Lazuka, Lucas Auer, Michael O'Donohue, Guillermina Hernandez-Raquet
BIOTECHNOLOGY FOR BIOFUELS
(2018)
Article
Biochemistry & Molecular Biology
Amel Taha, Melek Ben Aissa, Enshirah Da'na
Article
Chemistry, Organic
Olga Gherbovet, Fernando Ferreira, Apolline Clement, Melanie Ragon, Julien Durand, Sophie Bozonnet, Michael J. O'Donohue, Regis Faure
Summary: This study utilized a chemoenzymatic approach to synthesize different ferulate compounds, shortening synthetic routes and enhancing transesterification yields. A novel feruloylated compound was also successfully synthesized. These findings provide new substrate options for characterizing feruloyl esterases.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
David Teze, Jiao Zhao, Mathias Wiemann, Zubaida G. A. Kazi, Rossana Lupo, Birgitte Zeuner, Marlene Vuillemin, Mette E. Ronne, Goran Carlstrom, Jens O. Duus, Yves-Henri Sanejouand, Michael J. O'Donohue, Eva Nordberg Karlsson, Regis Faure, Henrik Stalbrand, Birte Svensson
Summary: This method describes a straightforward strategy involving rapid in silico analysis of protein sequences to identify single-mutant candidates for improving transglycosylation yields. Requiring minimal prior knowledge of the target enzyme, the method is generic and can validate mutations in one enzyme for transposition to others, even distantly related enzymes.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemical Research Methods
Jiao Zhao, Tobias Tandrup, Bastien Bissaro, Sophie Barbe, Jens-Christian N. Poulsen, Isabelle Andre, Claire Dumon, Leila Lo Leggio, Michael J. O'Donohue, Regis Faure
Summary: The study focused on exploring the active site of a GH51 retaining alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus through analysis of two mutants, F26L and L352M. The results showed that the mutant R69H-N216W-L352M displayed excellent transglycosylation activity by inducing changes in the donor and acceptor subsites. Additionally, the central role played by the conserved R69 residue in affecting the T/H partition was reaffirmed.
Article
Biochemistry & Molecular Biology
Jiao Zhao, Jeremy Esque, Isabelle Andre, Michael J. O'Donohue, Regis Faure
Summary: The GH-51 alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus exhibits versatile catalytic properties, with mutants showing increased yields and regioselectivity in the synthesis of furanobiosides. The study demonstrates the potential usefulness of these mutants for glycosynthesis, particularly in the production of biologically-relevant glycoconjugates. Through in-depth characterization, it was found that mutants can maximize the yield of homo-disaccharides by using higher substrate concentrations.
BIOORGANIC CHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
U. Javourez, M. O'Donhue, L. Hamelin
Summary: Residual biomass plays a crucial role in the transition towards circular and low fossil carbon economies by providing energy, chemicals, materials, and food products or services. The review presents various waste-to-nutrition pathways and proposes a systematic framework for waste conversion. The study also categorizes different pathways and introduces a multidimensional representation of biomass suitability for nutrition sources.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemical Research Methods
Louise Badruna, Vincent Burlat, Pierre Roblin, Thomas Enjalbert, Guy Lippens, Immacolata Venditto, Michael J. O'Donohue, Cedric Y. Montanier
Summary: This study focuses on investigating carbohydrate-binding modules using the Jo-In biomolecular welding protein pair. By creating recombinant xylanase variants appended to different carbohydrate-binding modules, the research reveals biochemical properties and lays the foundation for understanding the correlation between enzyme activity and substrate properties.
Article
Biotechnology & Applied Microbiology
Haiyang Wu, Eleni Ioannou, Bernard Henrissat, Cedric Y. Montanier, Sophie Bozonnet, Michael J. O'Donohue, Claire Dumon
Summary: The functional screening of a termite gut metagenomic library identified a xylan-degrading enzyme, Pm25, belonging to GH10 family. Sequence analysis revealed unique domain organization of Pm25, with upstream genes suggesting it as a Xyn10C-like enzyme. The enzyme's multimodularity and CBMs' role were further studied, showing synergistic effects of CBMs on enzymatic activity. These findings enhance understanding of Xyn10C-like proteins in gut bacteria's xylan utilization systems.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Gianluca Cioci, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
BIOTECHNOLOGY FOR BIOFUELS
(2017)
