Article
Biochemistry & Molecular Biology
Ivana Charousova, Miroslava Hlebova, Lukas Hleba, Juraj Medo, Joachim Wink
Summary: A new actinomycete producer of the antibiotic gamma-rubromycin, designated TA 36, was successfully identified as S. iakyrus through morphological, physiological and biochemical characteristics, phylogenetic analysis, and MALDI-TOF MS data. The crude extract of TA 36 showed strong antimicrobial activity against Staphylococcus aureus, and further analysis identified the presence of the promising antitumor and antibacterial antibiotic gamma-rubromycin. This study suggests that S. iakyrus TA 36 is the first-reported source of this unique bioactive secondary metabolite.
Article
Multidisciplinary Sciences
Essam Kotb, Amira H. Alabdalall, Azzah I. Alghamdi, Ibtisam M. Ababutain, Sumayh A. Aldakeel, Safa K. Al-Zuwaid, Batool M. Algarudi, Sakina M. Algarudi, Asmaa A. Ahmed, Ahmed M. Albarrag
Summary: Forty-six promising chitinolytic isolates were recovered in the environment of Saudi Arabia, with the top three isolates belonging to the genus Streptomyces. Streptomyces variabilis Am1 showed the highest chitinase activity at 84 hours, with 0.5% yeast extract and nitrogen source, and 2% galactose as carbon source. Purification of chitinase improved its specific activity and recovery, and revealed its metallo-chitinase type with ferric ions as cofactors. The chitinase demonstrated high antifungal activity against phytopathogenic fungi, and the tested Streptomyces were able to produce chitosan nanocubes, showing their additional power in antifungal activity. This work highlights the importance of unexplored environments for the discovery of promising microorganisms with biotechnological applications.
SCIENTIFIC REPORTS
(2023)
Article
Green & Sustainable Science & Technology
Tao Lang, Ping Xiang, Mingdang Li, Zhiteng Chen, Fenglan Li, Mingguo Jiang, Haichao Zhou
Summary: This study analyzed the chemical structures of tannins from mangrove plants using various extraction and purification methods. The results showed the influence of different methods on the characteristics of the tannins. The study identified the best methods for extracting and purifying tannins, providing accurate structural information for future research on mangrove tannins.
Article
Microbiology
Gaber Abo-Zaid, Ahmed Abdelkhalek, Saleh Matar, Mai Darwish, Muhammad Abdel-Gayed
Summary: The Actino 48 strain of Streptomyces cellulosae exhibited strong suppression of Sclerotium rolfsii mycelium growth and high chitinase enzyme production. By using a talc-based culture broth formulation, it effectively reduced the occurrence of peanut diseases and increased the yield, showing potential as a biocontrol agent for soil-borne diseases caused by S. rolfsii.
Article
Microbiology
Eva Gato, Ignacio Pedro Constanso, Bruno Kotska Rodino-Janeiro, Paula Guijarro-Sanchez, Tyler Alioto, Manuel Jesus Arroyo, Gema Mendez, Luis Mancera, Marta Gut, Ivo Gut, Miguel Alvarez-Tejado, German Bou, Marina Oviano
Summary: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for direct detection of KPC-producing isolates by analyzing the 11,109 Da mass peak representing the P019 protein. The study found the P019 peak prevalence in bla(KPC) K. pneumoniae isolates to be 49.2%, while it was absent in other carbapenemase or noncarbapenemase producers. Variability of P019 presence was observed in different geographical locations within the EuSCAPE collection, indicating a need for reconsideration when applying this technique in a clinical setting.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mizuho Suzuki, Kyosuke Takeshita, Yuki Kitamura, Marie Kuribayashi, Zhenlie Huang, Gaku Ichihara, Shinji Oikawa, Sahoko Ichihara
Summary: Diabetes mellitus is a pro-thrombotic state that can potentially cause serious cardiovascular complications. Platelet hyperactivation plays an important role in these pathological processes, however there is little or no information on the effect of hyperglycemia on platelet proteins. The aim of this study was to identify the molecular targets associated with platelet reactivity under hyperglycemia.
Article
Biochemistry & Molecular Biology
Kasumi Takeuchi, Masayo Ogiso, Tomohiro Morohoshi, Shigemi Seo
Summary: Glutamate enhances the biocontrol activity of Pseudomonas protegens Cab57 against Pythium damping off and root rot in cucumber by upregulating the expression of a chitinase gene cluster. The production levels of other biocontrol factors are reduced by glutamate. Glutamate plays an essential role in biocontrol activity where chitinase production is regulated.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Microbiology
Nadege C. Toney, Wenming Zhu, Bette Jensen, Jarrett Gartin, Karen Anderson, David Lonsway, Maria Karlsson, J. Kamile Rasheed
Summary: This study evaluated the ability of the Bruker Biotyper matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) platform to accurately identify 314 nontuberculous mycobacteria (NTM). The results showed that MALDI-TOF had an accuracy of 94% in identifying NTM, with higher accuracy for rapidly growing mycobacteria (99%) compared to slowly growing mycobacteria (87%). Additional sequencing of the rpoB gene was required for certain species to improve identification accuracy.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Microbiology
Eva Gato, Ignacio Pedro Constanso, Ana Candela, Fatima Galan, Bruno Kotska Rodino-Janeiro, Manuel Jesus Arroyo, Gema Mendez, Luis Mancera, Tyler Alioto, Marta Gut, Ivo Gut, Miguel Alvarez-Tejado, Belen Rodriguez-Sanchez, German Bou, Marina Oviano
Summary: The study aimed to standardize an operating procedure and data analysis for identification of CPK using MALDI-TOF MS. Analysis of 162 K. pneumoniae isolates showed that 100% of CPK isolates were correctly identified through a neural network analysis and applying TIC normalization. This demonstrates the feasibility of real-time tracking of CPK using MALDI-TOF MS.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Microbiology
Camila Morschbacher Wilhelm, Maiara dos Santos Carneiro, Everton Inamine, Afonso Luis Barth
Summary: This study aimed to evaluate an adaptation of the MBT-ASTRA technique for faster antimicrobial susceptibility testing. The adapted method showed high categorical agreement with the standard method, indicating its potential for rapid AST results.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
G. Guo, M. Papanicolaou, N. J. Demarais, Z. Wang, K. L. Schey, P. Timpson, T. R. Cox, A. C. Grey
Summary: Spatial proteomics using MALDI-MSI is a powerful tool for direct detection of protein distribution in tissues but faces challenges in peptide quantification and identification. The development of HIT-MAP provides an open-source bioinformatics workflow for accurate protein annotation and spatial mapping from MALDI-MSI data.
NATURE COMMUNICATIONS
(2021)
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Article
Medicine, General & Internal
Jian Zhang, Zhizhong Liu, Ribing Chen, Qingwei Ma, Qian Lyu, Shuhui Fu, Yufei He, Zijie Xiao, Zhi Luo, Jianming Luo, Xingyu Wang, Xiangyi Liu, Peng An, Wei Sun
Summary: This study constructed a machine learning model based on MALDI-TOF mass spectrometry to screen for thalassaemia, achieving good classification performance and showing great potential for rapid screening in large populations.
ANNALS OF MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Chenyin Lv, Tianyan Gu, Rui Ma, Wei Yao, Yuyang Huang, Jingang Gu, Guogang Zhao
Summary: SaChiB, a chitinase of GH family 19, displays optimal activity at 45°C and pH 8.0, with good stability. It exhibits substrate specificity towards chitin and chitooligosaccharides and has potential applications in industrial waste conversion and agricultural biocontrol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Jie Wang, Bingyu Cai, Kai Li, Yankun Zhao, Chunyu Li, Siwen Liu, Dandan Xiang, Lu Zhang, Jianghui Xie, Wei Wang
Summary: Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense is a disastrous fungal disease. Strain WHL7 of Streptomyces sp. isolated from marine soft coral showed high antifungal activity against Foc TR4. Fermentation broth of Streptomyces sp. WHL7 significantly increased the resistance of banana plantlets to Foc TR4, making it a potential bioresource for controlling Foc TR4.