Journal
JOURNAL OF BASIC MICROBIOLOGY
Volume 53, Issue 1, Pages 98-100Publisher
WILEY-BLACKWELL
DOI: 10.1002/jobm.201100539
Keywords
Allomerus ants; Black fungi; Chaetothyriales; Fluorescent staining; Isolation method
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Funding
- French Agence Nationale de la Recherche [ANR-06-JCJC-0109-01]
- ESF-EURO-CORES/TECT/BIOCONTRACT program [06-TECT-FP-007]
- Fondation pour la Recherche sur la Biodiversite [AAP-IN-2009-050]
- Programme Interface Physique, Chimie, Biologie of the French Centre National de la Recherche Scientifique (CNRS)
- Nouragues research grant from the CNRS
- FSE (Fond Social Europeen)
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Because of their ecological characteristics, slow growth rates and the presence of contaminants, Chaetothyriales fungi associated with structures built by tropical plant-ants can be difficult to isolate with standard procedures. Here, we describe an easy-to-use protocol for obtaining pure cultures by using cotton as a first substrate. We have further found by means of fluorescent stains that nuclei concentrate either in young hyphae or in the tips of the hyphae.
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