Article
Immunology
Minjing Wu, Shan Huang, Jingyun Du, Yijun Li, Shan Jiang, Ling Zhan, Xiaojing Huang
Summary: This study investigates the impact of D-alanylation of Lipoteichoic acid (LTA) on biofilm formation and acidogenesis of Streptococcus mutans (S. mutans). The research found that blocking the D-alanylation of LTA disrupts normal biofilm formation in S. mutans by altering intercellular auto-aggregation, cell adhesion, and extracellular matrix formation. It also suggests that LTA D-alanylation plays an important role in S. mutans acidogenesis by altering glycolytic activity.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Matthew E. Turner, Khanh Huynh, Ronan K. Carroll, Sang-Joon Ahn, Kelly C. Rice
Summary: The study revealed that Streptococcus mutans utilizes a RibU-like riboflavin transporter (SMU.1703c) to acquire vitamin B2, affecting its growth in complex environments and acid stress tolerance.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Chiau-Jing Jung, Chih-Chieh Hsu, Jeng-Wei Chen, Hung-Wei Cheng, Chang-Tsu Yuan, Yu-Min Kuo, Ron-Bin Hsu, Jean-San Chia
Summary: The LiaR-regulated PCP protein in Streptococcus mutans plays a crucial role in bacterial biofilm formation and platelet adhesion. It is well-preserved among pathogenic streptococci and controls biofilm formation in vivo, suggesting its importance in colonization for S. mutans and possibly other pathogenic streptococci.
Article
Biochemistry & Molecular Biology
Tohid Rezaei, Fadhil S. Kamounah, Ehsaneh Khodadadi, Bahareh Mehramouz, Pourya Gholizadeh, Leila Yousefi, Khudaverdi Ganbarov, Reza Ghotaslou, Mehdi Yousefi, Mohammad Asgharzadeh, Hosein Eslami, Sepehr Taghizadeh, Tahereh Pirzadeh, Hossein Samadi Kafil
Summary: This study aimed to assess the influence of sucrose and starch on biofilm formation and proteome profile of Streptococcus mutans ATCC 35668 strain, which is a main organism of tooth infections. The results showed that carbohydrates can increase microbial pathogenicity and induce biofilm formation of S. mutans via changes in the expression of special proteins.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Microbiology
So-Young Ham, Han-Shin Kim, Eunji Cha, Taehyeung Lim, Youngjoo Byun, Hee-Deung Park
Summary: Raffinose, a natural oligosaccharide, strongly inhibits biofilm formation and GTF-related gene expression in Streptococcus mutans, making it a potential antibiofilm agent for oral supplies and dental materials to prevent dental caries.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agriculture, Multidisciplinary
Ji-Eun Lee, Hee Yeon Cho, Jae Hoon Lee, Dong Uk Ahn, Kee-Tae Kim, Hyun-Dong Paik
Summary: This study investigated the inhibitory effects of ovomucoid, a protein found in egg white, on the biofilm formation of Streptococcus mutans, the primary pathogen associated with tooth decay. The results showed that ovomucoid inhibited biofilm formation, reduced bacterial adhesion and aggregation, and lowered the metabolic activity of bacteria in the biofilm. In addition, ovomucoid decreased the synthesis of water-insoluble EPS by S. mutans. Therefore, ovomucoid has the potential to be used as an anti-biofilm agent for the treatment of dental caries.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Immunology
Shota Mayumi, Masae Kuboniwa, Akito Sakanaka, Ei Hashino, Asuka Ishikawa, Yura Ijima, Atsuo Amano
Summary: Recent studies have shown phenotypic and metabolic heterogeneity in related species including Streptococcus oralis, Streptococcus mutans, and Streptococcus gordonii. D-tagatose was found to be abundant in the saliva of individuals with good oral hygiene, and it selectively inhibited the growth of oral pathogens S. mutans and S. gordonii while minimally affecting the oral commensal S. oralis. This suggests the potential use of D-tagatose as an oral prebiotic for manipulating the metabolism of targeted oral streptococci.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Rodrigo A. Giacaman, Rodrigo Umana, Maria Jose Nunez, Natalia Diaz-Garrido, Constanza Echeverria, Natalia Garcia-Manriquez, Alex Mira, Constanza E. Fernandez, Karla Gambetta-Tessini, Carla P. Lozano
Summary: This study aimed to investigate the impact of saliva dilutions on an in vitro caries model using Streptococcus mutans biofilms. The results showed that even small quantities of saliva could significantly reduce demineralization in enamel and dentin, and the protective effect of saliva was dose-dependent.
Article
Microbiology
Muna Aqawi, Ronit Vogt Sionov, Ruth Gallily, Michael Friedman, Doron Steinberg
Summary: The study demonstrated that Cannabigerol (CBG) can effectively prevent the formation of Streptococcus mutans biofilms, reduce biofilm thickness and metabolic activity, and alter the surface roughness of the biofilms. CBG also decreased the expression of genes related to S. mutans biofilm formation, indicating its potential as a preventive treatment for dental caries.
Article
Food Science & Technology
Guofang Zhang, Ying Tan, Tianshu Yu, Sitong Wang, Libo Liu, Chun Li
Summary: The combination of reuterin and catechin shows synergistic antimicrobial activity against Streptococcus mutans by inhibiting growth, biofilm formation, water-insoluble glucan production, and virulence genes expression. This novel combination demonstrates potential as a natural antibacterial agent for the prevention of oral diseases.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Immunology
Xiuqin Chen, Akanksha Tyagi, Selvakumar Vijayalakshmi, Ramachandran Chelliah, Umair Shabbir, Deog-Hwan Oh
Summary: This study found that the combination of slightly acidic electrolyzed water (SAEW) and sodium benzoate (NaB) can effectively control Streptococcus mutans biofilm, reducing its adhesive strength and decreasing the height of the biofilm. This could be a promising new approach for treating dental caries.
MICROBIAL PATHOGENESIS
(2022)
Article
Biotechnology & Applied Microbiology
Luting Weng, Lang Wu, Rongjuan Guo, Jiajia Ye, Wen Liang, Wei Wu, Liang Chen, Deqin Yang
Summary: This study successfully prepared nanoparticles, called LA/TCS@PLGA-NPs, with favorable properties using cell membrane coating technology and Lactobacillus strains. The nanoparticles were able to adhere to Streptococcus mutans and interfere with its biofilm formation, leading to significant inhibition of the activity, biomass, and virulence gene expression of the biofilms. In vivo experiments also showed that LA/TCS@PLGA-NPs had a long-lasting inhibitory effect on the progression of dental caries. These nanoparticles could be considered as a new class of effective drug delivery systems for caries prevention.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yuting Tian, Yue Zhang, Mengjiao Zhang, Xianchun Chen, Lei Lei, Tao Hu
Summary: The purpose of this study was to construct a suitable vector to carry and protect antisense vicR RNA (ASvicR), and investigate its effects on the cariogenicity of S. mutans. Aminated dendritic mesoporous silica nanoparticles (DMSNs-NH2) were found to be a suitable carrier for ASvicR, reducing EPS synthesis and biofilm formation of S. mutans.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Zhimin Hu, Ying Tang, Bulin Jiang, Yue Xu, Siying Liu, Cui Huang
Summary: This study aimed to avoid antibiotic resistance by using curcumin, a natural plant extract with photodynamic effects, to exhibit antibacterial activity against Streptococcus mutans (S. mutans) biofilms. To overcome the limitations of curcumin, researchers constructed a curcumin-loaded liposome with high drug loading efficiency, stability, controlled release, and biocompatibility. The results showed that the liposome effectively adhered to and inhibited the growth of S. mutans biofilms. Therefore, curcumin-loaded liposomes have the potential for clinical application in the treatment of dental caries.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Li Yan, Sha Zhang, Xiaoying Zhou, Shuge Tian
Summary: This study investigated the antibacterial ability and inhibitory mechanisms of orientin-2''-O-beta-L-galactoside, orientin, and vitexin against Streptococcus mutans. The results showed that these natural flavonoids had inhibitory effects on S. mutans and reduced EPS formation while stimulating the release of LDH. They also inhibited biofilm formation and down-regulated the transcription levels of various genes in S. mutans.