Article
Microbiology
Miguel Diaz-Guerrero, Meztlli O. Gaytan, Eduardo Soto, Norma Espinosa, Elizabeth Garcia-Gomez, Arely Marcos-Vilchis, Angel Andrade, Bertha Gonzalez-Pedrajo
Summary: The type III secretion system (T3SS) is a complex molecular device that enables pathogenic bacteria to transport effector proteins directly into host cells. The CesL/SepL/SepD complex regulates the switch from translocator to effector secretion in enteropathogenic Escherichia coli, ensuring proper secretion and stability of the components involved. CesL also interacts with the cytoplasmic domains of export gate components, contributing to the regulation of substrate secretion.
Article
Biochemistry & Molecular Biology
Jorge Soria-Bustos, Zeus Saldana-Ahuactzi, Partha Samadder, Jorge A. Yanez-Santos, Ygnacio Martinez Laguna, Maria L. Cedillo-Ramirez, Jorge A. Giron
Summary: This study investigates the relationship between flagella expression and the type III secretion system (T3SS) in enteropathogenic Escherichia coli (EPEC). The results suggest that the assembly of an intact T3SS is required for flagella production, and certain regulator genes play a role in activating or inhibiting flagella expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Microbiology
Liam J. Worrall, Dorothy D. Majewski, Natalie C. J. Strynadka
Summary: This article describes two fascinating bacterial nanomachines - the widely distributed rotary flagellum and the eukaryotic cell-puncturing injectisome. Both nanomachines utilize the type III secretion system (T3SS) to secrete components and translocate proteins. Advances in structural and functional understanding, facilitated by cryoelectron microscopy technologies, have provided insights into these nanomachines since 2015.
ANNUAL REVIEW OF MICROBIOLOGY
(2023)
Article
Immunology
Shoichi Tachiyama, Ryan Skaar, Yunjie Chang, Brittany L. Carroll, Meenakumari Muthuramalingam, Sean K. Whittier, Michael L. Barta, Wendy L. Picking, Jun Liu, William D. Picking
Summary: Shigella flexneri, the causative agent of bacillary dysentery, utilizes a type III secretion system as its primary virulence factor to inject effector proteins into host cells. The cytoplasmic sorting platform of the injectisome, specifically the Spa33 pods, plays a critical role in substrate selection and secretion energizing. Through biophysical analyses, a model of Spa33 heterotrimers within the SP pods is proposed, suggesting how two distinct complexes come together to form complete pod structures during the recruitment of T3SS secretion substrates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Ashok R. Nayak, Pradip K. Singh, Jinlei Zhao, Montserrat Samso, Michael S. Donnenberg
Summary: Type 4 pili are important surface appendages on bacteria and archaea that can extend and retract with great force. This study presents the detailed structure of an enzyme that converts chemical energy to mechanical energy in a human pathogen, revealing a mechanism of action not previously seen in enzymes that power type 4 pilus extension.
Article
Biochemistry & Molecular Biology
Owain J. Bryant, Gillian M. Fraser
Summary: The flagellar T3SS export gate opens and closes in response to export substrate availability, energized by FlhA in a proton motive force-dependent manner. The export substrate and the FliJ stalk of the flagellar ATPase provide non-redundant gate-activating signals critical for efficient export.
Article
Microbiology
Anish Zacharia, Ritesh Ranjan Pal, Naama Katsowich, Chanchal Thomas Mannully, Aida Ibrahim, Sivan Alfandary, Raphael Serruya, Amit K. Baidya, Sigal Ben-Yehuda, Ilan Rosenshine, Arieh Moussaieff
Summary: The lipid composition of the bacterial envelope undergoes remodeling upon attachment to host cells and activation of the type III secretion system (T3SS), which is associated with increased bacterial fitness and sensitivity to antibiotics. This study provides important insights into the shift of pathogenic bacteria, such as enteropathogenic Escherichia coli (EPEC), from a planktonic to adherent lifestyle.
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Editorial Material
Multidisciplinary Sciences
Itzhak Fishov, Sharanya Namboodiri
Summary: The type three secretion system (T3SS) is a membrane-anchored nano-machine used by pathogenic bacteria to inject effector proteins and control host cells. Recent research shows the colocalization of T3SS-encoding locus, its transcriptional activators, protein products, and the complete structure at the cell membrane, providing evidence for a mechanism called 'transertion'.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Kazuki Kato, Sae Okazaki, Cian Schmitt-Ulms, Kaiyi Jiang, Wenyuan Zhou, Junichiro Ishikawa, Yukari Isayama, Shungo Adachi, Tomohiro Nishizawa, Kira S. Makarova, Eugene V. Koonin, Omar O. Abudayyeh, Jonathan S. Gootenberg, Hiroshi Nishimasu
Summary: The type III-E CRISPR-Cas7-11 effector can catalyze crRNA-guided RNA cleavage by binding crRNA and Csx29, and the binding of tgRNA induces conformational changes in Csx29. Csx29 cleaves Csx30 through a tgRNA-dependent pathway, resulting in the generation of toxic protein fragments and growth arrest, which is regulated by Csx31. Csx30 modulates the cellular response to infection by binding Csx31 and the associated sigma factor RpoE. Additionally, the Cas7-11-Csx29-Csx30 system has been engineered for programmable RNA sensing in mammalian cells.
Article
Veterinary Sciences
Yaodong Zhang, Yao Wang, Hong Zhu, Zhengfei Yi, Dosseh Jean Apotre Afayibo, Chenglin Tao, Tao Li, Mingxing Tian, Jingjing Qi, Chan Ding, Shengqing Yu, Shaohui Wang
Summary: The inactivation of the DctR gene in APEC resulted in decreased biofilm formation, reduced adherence capacity, and other negative effects, which were restored through genetic complementation. Additionally, DctR positively regulates the expression of ETT2 in APEC, influencing the virulence of the pathogen.
VETERINARY RESEARCH
(2021)
Review
Biology
Dipasree Hajra, Abhilash Vijay Nair, Dipshikha Chakravortty
Summary: Various Gram-negative bacteria possess a specialized membrane-bound protein secretion system known as the Type III secretion system (T3SS), which transports bacterial effector proteins into host cells to aid in pathogenesis. Studies have utilized various techniques to investigate the structure and function of T3SS and how different human pathogenic bacteria manipulate host systems to enhance virulence.
PHYSICS OF LIFE REVIEWS
(2021)
Article
Biology
Owain J. Bryant, Paraminder Dhillon, Colin Hughes, Gillian M. Fraser
Summary: In addition to the gate recognition motif (GRM), a hydrophobic N-terminal signal is identified on flagellar rod and hook subunits, which are separately and sequentially recognized by the flagellar export machinery. The hydrophobic N-terminal signal may trigger the opening of the flagellar export gate.
Article
Biochemistry & Molecular Biology
Bailey Milne-Davies, Stephan Wimmi, Andreas Diepold
Summary: The type III secretion system is the common core of two bacterial molecular machines: the flagellum and the injectisome. It is characterized by flexibility, adaptivity, and a dynamic nature.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Elizabeth Garcia-Gomez, Jesus F. T. Miranda-Ozuna, Francisco Diaz-Cedillo, Ernesto A. Vazquez-Sanchez, Sandra Rodriguez-Martinez, Janet Jan-Roblero, Mario E. Cancino-Diaz, Juan Carlos Cancino-Diaz
JOURNAL OF MEDICAL MICROBIOLOGY
(2017)
Article
Immunology
Elizabeth Garcia-Gomez, Marcos E. Jaso-Vera, Marco A. Juarez-Verdayes, Maria D. Alcantar-Curiel, Juan C. Zenteno, Gabriel Betanzos-Cabrera, Humberto Peralta, Sandra Rodriguez-Martinez, Mario E. Cancino-Diaz, Janet Jan-Roblero, Juan C. Cancino-Diaz
MICROBIAL PATHOGENESIS
(2017)
Article
Microbiology
Meztlli O. Gaytan, Julia Monjaras Feria, Eduardo Soto, Norma Espinosa, Julia M. Benitez, Dimitris Georgellis, Bertha Gonzalez-Pedrajo
Review
Endocrinology & Metabolism
Edgar Ricardo Vazquez-Martinez, Elizabeth Garcia-Gomez, Ignacio Camacho-Arroyo, Bertha Gonzalez-Pedrajo
BIOLOGY OF SEX DIFFERENCES
(2018)
Article
Biochemistry & Molecular Biology
Xiang Ruan, Julia Monjaras Feria, Mohamad Hamad, Miguel A. Valvano
MOLECULAR MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Alejandra Martinez-Ibarra, Luis Daniel Martinez-Razo, Edgar Ricardo Vazquez-Martinez, Nayeli Martinez-Cruz, Rogelio Flores-Ramirez, Elizabeth Garcia-Gomez, Marisol Lopez-Lopez, Carlos Ortega-Gonzalez, Ignacio Camacho-Arroyo, Marco Cerbon
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Review
Developmental Biology
Edgar Ricardo Vazquez-Martinez, Yadira Ines Gomez-Viais, Elizabeth Garcia-Gomez, Christian Reyes-Mayoral, Enrique Reyes-Munoz, Ignacio Camacho-Arroyo, Marco Cerbon
Article
Medicine, Research & Experimental
Elizabeth Garcia-Gomez, Mariana Bobadilla-Bravo, Eulises Diaz-Diaz, Edgar Ricardo Vazquez-Martinez, Sonia Nava-Salazar, Yessica Torres-Ramos, Carmen Selene Garcia-Romero, Ignacio Camacho-Arroyo, Marco Cerbon
CURRENT MOLECULAR MEDICINE
(2020)
Review
Endocrinology & Metabolism
Elizabeth Garcia-Gomez, Edgar Ricardo Vazquez-Martinez, Christian Reyes-Mayoral, Oliver Paul Cruz-Orozco, Ignacio Camacho-Arroyo, Marco Cerbon
FRONTIERS IN ENDOCRINOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Edgar Ricardo Vazquez-Martinez, Claudia Bello-Alvarez, Ana Lorena Hermenegildo-Molina, Mario Solis-Paredes, Sandra Parra-Hernandez, Oliver Cruz-Orozco, J. Roberto Silvestri-Tomassoni, Luis F. Escobar-Ponce, Luis A. Hernandez-Lopez, Christian Reyes-Mayoral, Andrea Olguin-Ortega, Brenda Sanchez-Ramirez, Mauricio Osorio-Caballero, Elizabeth Garcia-Gomez, Guadalupe Estrada-Gutierrez, Marco Cerbon, Ignacio Camacho-Arroyo
BIOMED RESEARCH INTERNATIONAL
(2020)
Review
Immunology
Julia Monjaras Feria, Miguel A. Valvano
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Microbiology
Miguel Diaz-Guerrero, Meztlli O. Gaytan, Eduardo Soto, Norma Espinosa, Elizabeth Garcia-Gomez, Arely Marcos-Vilchis, Angel Andrade, Bertha Gonzalez-Pedrajo
Summary: The type III secretion system (T3SS) is a complex molecular device that enables pathogenic bacteria to transport effector proteins directly into host cells. The CesL/SepL/SepD complex regulates the switch from translocator to effector secretion in enteropathogenic Escherichia coli, ensuring proper secretion and stability of the components involved. CesL also interacts with the cytoplasmic domains of export gate components, contributing to the regulation of substrate secretion.
Review
Developmental Biology
Diana Medina-Bastidas, Ignacio Camacho-Arroyo, Elizabeth Garcia-Gomez
Summary: The microbiota in the uterus plays a crucial role in maintaining reproductive health, and dysbiosis of the uterine microbiome is associated with various uterine diseases. Specific alterations in the bacterial community, such as a decrease in Firmicutes and Lactobacillus and an increase in Proteobacteria, Bacteroidetes, and Actinobacteria, are observed in diseased endometrium compared to healthy endometrium. The components of the endometrial microbiota show promise as biomarkers for diagnosing uterine diseases and have potential as probiotics to complement existing therapies.
Article
Gastroenterology & Hepatology
Violeta Valdovinos-Bello, Carmen Selene Garcia-Romero, Alicia Cervantes-Peredo, Elizabeth Garcia-Gomez, Alejandra Martinez-Ibarra, Edgar Ricardo Vazquez Martinez, Yolotzin Valdespino, Marco Cerbon
Summary: This study aimed to evaluate placental histopathological changes in patients with intrahepatic cholestasis of pregnancy (ICP) and explore correlations between these changes and hepatic function alteration, patient comorbidities, and body mass index. The results showed that the placenta of ICP patients exhibited numerous histopathological alterations, but no correlations were found between the increase in bile acids or liver functional parameters and specific placental lesions. The most frequent comorbidities in ICP patients were obesity, overweight, and preeclampsia. It is necessary to improve the medical strategies for the treatment and monitoring of liver dysfunction in ICP patients.
ANNALS OF HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Elizabeth Garcia-Gomez, Yadira Ines Gomez-Viais, Martin Mizael Cruz-Aranda, Luis Daniel Martinez-Razo, Christian Reyes-Mayoral, Lizeth Ibarra-Gonzalez, Araceli Montoya-Estrada, Mauricio Osorio-Caballero, Otilia Perichart-Perera, Ignacio Camacho-Arroyo, Marco Cerbon, Enrique Reyes-Munoz, Edgar Ricardo Vazquez-Martinez
Summary: This study investigated the expression and DNA methylation levels of eight genes related to endometrial function in the endometrium of PCOS patients and control group. The results showed decreased expression of HOXA10, GAB1, and SLC2A4 genes and increased DNA methylation levels of the HOXA10 promoter in PCOS patients. After metformin and nutritional intervention, improvements were observed in metabolic and clinical variables, accompanied by increased expression of HOXA10, ESR1, GAB1, and SLC2A4 genes and reduced DNA methylation levels of the HOXA10 promoter in PCOS women. These findings suggest that metformin and a carbohydrate-controlled diet can improve endometrial function in PCOS patients by modulating DNA methylation and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)