Article
Food Science & Technology
Wenyan Xie, Yuxian You, Xiaofeng Ban, Anqi Zhang, Caiming Li, Zhengbiao Gu, Zhaofeng Li
Summary: In this study, a cold-activated agarase with outstanding cold adaptation was cloned and expressed. The enzyme exhibited enhanced activity after low-temperature incubation and retained high activity at low temperatures. Structural changes were observed in the enzyme after low-temperature treatment. The carbohydrate-binding modules and linker region were found to play a crucial role in cold adaptation.
Article
Chemistry, Medicinal
Xun-Ke Sun, Ya Gong, Dan-Dan Shang, Bang-Tao Liu, Zong-Jun Du, Guan-Jun Chen
Summary: Alginate is a major component of brown algae, crucial in marine ecosystems. In this study, 100 alginate-degrading strains were isolated from adult abalones, with Agarivorans sp. B2Z047 exhibiting the strongest degradation ability. Whole genome sequencing identified 12 potential alginate lyase genes, revealing the role of these lyases in the alginate degradation process.
Article
Biotechnology & Applied Microbiology
Duleepa Pathiraja, Line Christiansen, Byeonghyeok Park, Mikkel Schultz-Johansen, Geul Bang, Peter Stougaard, In-Geol Choi
Summary: Marine microorganisms encode a complex repertoire of carbohydrate-active enzymes for the catabolism of algal cell wall polysaccharides, with a diversified agarolytic gene repertoire in marine bacteria. Colwellia echini A3(T) harbors two less-abundant GH96 a-agarases in its agar-specific polysaccharide utilization locus, which contribute to the metabolic versatility of the agarolytic pathway. The expansion of the agarolytic gene repertoire and the novel hydrolytic functions, such as the newly elucidated molecular functionality of alpha-agarase, promote metabolic versatility and competitive abilities in marine agarolytic bacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mingjing Mou, Qingsong Hu, Hebin Li, Liufei Long, Zhipeng Li, Xiping Du, Zedong Jiang, Hui Ni, Yanbing Zhu
Summary: In this study, a new member of chondroitinase B, ChSase B6, was cloned and characterized. The recombinant enzyme showed optimal activity at 40°C and pH 8.0, and demonstrated thermostability and pH stability. ChSase B6 also displayed stability against surfactants and improved the antioxidant ability of chondroitin sulfate. Structural analysis and mutagenesis suggested the importance of specific residues for the activity of ChSase B6. The characterization and structure of ChSase B6 provided guidance for its industrial application in chondroitin sulfate bioresource development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Jingjing Shen, Guangning Chen, Yuying Zhang, Xuanwei Mei, Yaoguang Chang, Changhu Xue
Summary: In recent decades, there has been increasing research interest in sulfated fucans due to their various physiological activities. Fucanases are essential for studying sulfated fucans. In this study, a novel GH168 family endo1,3-fucanase was cloned from the genome of the marine bacterium Wenyingzhuangia fucanilytica. The enzyme, named Fun168D, was found to cleave specific bonds in sulfated fucans from Isostichopus badionotus and Holothuria tubulosa, revealing the presence of diversity within the GH168 family.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Medicinal
Youngdeuk Lee, Eunyoung Jo, Yeon-Ju Lee, Tae-Yang Eom, Yehui Gang, Yoon-Hyeok Kang, Svini Dileepa Marasinghe, Sachithra Amarin Hettiarachchi, Do-Hyung Kang, Chulhong Oh
Summary: A new beta-agarase, Gaa16B, was identified and characterized for its activity and stability in agarose hydrolysis. The study also found that the partial hydrolysis products of Gaa16B exhibited high inhibition activity against hyaluronidase.
Article
Microbiology
Lei Shi, Kunlian Mo, Shixiang Bao, Qingjuan Wu, Chunmei Xue, Huiqin Huang
Summary: The bacterium HB182678(T) isolated from brown algae in Hainan province, China, belongs to the genus Mangrovicoccus and is proposed as a new species, Mangrovicoccus algicola sp. nov. It shows a wide range of growth conditions in terms of temperature, pH, and salt concentration, with specific isoprenoid quinone and fatty acid components.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Olivia M. Gruenzweil, Lauren Palmer, Adriana Cabal, Michael P. Szostak, Werner Ruppitsch, Christian Kornschober, Maciej Korus, Dusan Misic, Tanja Bernreiter-Hofer, Anna D. J. Korath, Andrea T. Fessler, Franz Allerberger, Stefan Schwarz, Joachim Spergser, Elke Mueller, Sascha D. Braun, Stefan Monecke, Ralf Ehricht, Chris Walzer, Hrvoje Smodlaka, Igor Loncaric
Summary: The study aimed to investigate the presence of ESBL- and AmpC-producing Enterobacterales, as well as Salmonella, in marine mammals. Results revealed a high prevalence of multidrug-resistant bacteria and pandemic high-risk clones, indicating anthropogenic antimicrobial pollution in these animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Chi Young Hwang, Sung Man Seo, Eui-Sang Cho, Young-Do Nam, So-Lim Park, Seong-Il Lim, Myung-Ji Seo
Summary: In this study, a novel bacterial strain MBLB1776(T) was isolated from marine mud in Uljin, the Republic of Korea. The strain was identified as a new species within the genus Paenibacillus, named Paenibacillus aurantius sp. nov. It was found that MBLB1776(T) produced carotenoid pigments, giving the cells an orange color.
Article
Chemistry, Medicinal
Sun Wook Jeong, Jung Eun Yang, Yong Jun Choi
Summary: In this study, a yellow xanthophyll pigment-producing marine bacterium, identified as the Erythrobacter sp. SDW2 strain, was isolated from coastal seawater. The SDW2 strain can efficiently produce yellow xanthophyll pigment and exhibits remarkable antioxidative activities. These results suggest that the SDW2 strain could be a promising industrial microorganism for producing marine-derived bioactive compounds.
Article
Environmental Sciences
Dorra Hentati, Meriam Cheffi, Fatma Hadrich, Neila Makhloufi, Francesc Rabanal, Angeles Manresa, Sami Sayadi, Mohamed Chamkha
Summary: Halotolerant strain CO100 of Staphylococcus sp. isolated from contaminated sediments in Tunisia showed efficient hydrocarbon degradation ability and synthesized a lipopeptide biosurfactant BS-CO100 with strong tensioactive properties under various conditions, non-cytotoxic to human cells. BS-CO100 also exhibited anti-biofilm and anti-adhesive activities, making it a promising candidate for bioremediation of oil-polluted areas, even under saline conditions.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Microbiology
Shaikh Rajesh Ali, Syeda Sagufta Sultana, Sisir Rajak, Prosun Tribedi, Sibani Sen Chakraborty
Summary: Lipase is an important enzyme in industries, and this study identified a microorganism strain with efficient lipase activity. This strain showed the highest lipase production at specific temperature and pH conditions, and exhibited high thermostability. However, further purification and commercial application of the enzyme require more work.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sethuramalingam Balakrishnan, Narasingam Arunagirinathan, Marimuthu Ragavan Rameshkumar, Purushothaman Indu, Nallusamy Vijaykanth, Khalid S. Almaary, Saeedah Musaed Almutairi, Tse-Wei Chen
Summary: This study isolated and identified seven bacterial strains from petroleum hydrocarbon contaminated sediments. The strain ADY2b showed promising results in biosurfactant production and demonstrated the ability to increase the bioavailability of poorly soluble petroleum hydrocarbons.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Athmika, Sudeep D. Ghate, A. B. Arun, Sneha S. Rao, S. T. Arun Kumar, Mrudula Kinarulla Kandiyil, Kanekar Saptami, P. D. Rekha
Summary: The Halomonas malpeensis strain YU-PRIM-29(T) is a halophilic bacterium that produces yellow pigment, exopolysaccharide (EPS), and industrially important metabolites such as zeaxanthin pigment, ectoine, and polyhydroxyalkanoate (PHA). Genome analysis revealed genes involved in EPS biosynthesis and other important metabolic pathways. The EPS showed strong emulsifying activities against petroleum hydrocarbons and its production was influenced by the carbon source.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Luis Alberto Villalba, Minoru Kasada, Luca Zoccarato, Sabine Wollrab, Hans Peter Grossart
Summary: Protist grazing pressure has a significant impact on controlling bacterial populations and affecting energy flow and biogeochemical cycles in aquatic environments. The escape mechanisms of bacteria in response to predation are not well studied. This research investigated the response of the marine bacterium Marinobacter adhaerens to different protist predators and found that the bacterium showed different escape strategies depending on the type of predator. It highlights the importance of behavioral escape mechanisms in community composition and energy flow in pelagic environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
