Article
Zoology
Lu-Chao Lv, Yao-Yao Lu, Xun Gao, Wan-Yun He, Ming-Yi Gao, Kai-Bin Mo, Jian-Hua Liu
Summary: This study characterized NDM-5-producing Enterobacteriaceae from aquatic products in Guangzhou, China, identifying bla(NDM-5) in Citrobacter freundii and Escherichia coli strains on various plasmids. The transfer of bla(NDM-5) between different plasmids was likely facilitated by the Tn7051 transposon, expanding the transmission vectors of this carbapenemase gene.
ZOOLOGICAL RESEARCH
(2022)
Article
Immunology
Pan Fu, Xinpeng Luo, Jun Shen, Leiyan He, Hua Rong, Chunling Li, Saige Chen, Lei Zhang, Aimin Wang, Chuanqing Wang
Summary: This study investigated carbapenemase-producing Enterobacteriaceae (CPE) strains isolated from children in Shanghai, China from 2016 to 2021. The results showed that NDM was the primary carbapenemase in children (67.6%), followed by KPC (26.4%), IMP (5.3%), and OXA-48 (0.6%). NDM-Kpn posed the most serious threat to infants, especially neonates, and the high-risk clone ST11 KPC-Kpn should be continuously monitored in children.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Microbiology
Mojtaba Shahin, Ali Ahmadi
Summary: The study investigated 29 carbapenem-resistant Pseudomonas aeruginosa isolates carrying the bla(NDM-1) gene in different cities of Iran. The isolates showed resistance to imipenem and ertapenem and carried other MBL encoding genes. DLST typing revealed partial diversity among the isolates, with DLST 25-11 cluster having the most infected or colonized patients.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Biochemistry & Molecular Biology
Kairui Yue, Chen Xu, Zhihao Wang, Wandong Liu, Chenyu Liu, Ximing Xu, Yan Xing, Sheng Chen, Xiaoyang Li, Shengbiao Wan
Summary: This study designed and developed a novel scaffold of benzo[d][1,2]selenazol-3(2H)-one derivatives, and the representative compound 15l showed strong antibacterial activity when combined with meropenem, with the potential to restore the antibacterial effect against clinically resistant Enterobacteriaceae strains.
BIOORGANIC CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Hongfa Lv, Zihao Zhu, Chenliang Qian, Tianlei Li, Zunsheng Han, Wenxuan Zhang, Xinxin Si, Jianfeng Wang, Xuming Deng, Li Li, Tianqi Fang, Jie Xia, Song Wu, Yonglin Zhou
Summary: In this study, a novel NDM-1 inhibitor called Zndm19 was identified through virtual screening and enzyme activity inhibition assay. It showed synergistic bactericidal efficacy when combined with meropenem in vitro and effectively combated bacterial infection in a mice peritonitis infection model. The important amino acid residues engaged in Zndm19 binding were also uncovered through molecular docking and mutagenesis. This research validates the potential of Zndm19 as a drug to treat drug-resistant bacterial infections, particularly those with NDM-1.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Microbiology
Satomi Takei, Yu Jie Lu, Mari Tohya, Shin Watanabe, Shigeki Misawa, Yoko Tabe, Takashi Miida, San Mya, Htay Htay Tin, Tatsuya Tada, Teruo Kirikae
Summary: This study demonstrates the spread of carbapenem-resistant K. pneumoniae strains in medical settings in Myanmar. Specifically, plasmid genes encoding NDMs and 16S rRNA methylases have been spreading in high-risk clones of K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Fakhur Uddin, Syed Hadi Imam, Saeed Khan, Taseer Ahmed Khan, Zulfiqar Ahmed, Muhammad Sohail, Ashraf Y. Elnaggar, Ahmed M. Fallatah, Zeinhom M. El-Bahy
Summary: The worldwide spread and increasing prevalence of carbapenem-resistant Enterobacteriaceae (CRE) is of utmost concern and a problem for public health, as determined by this study that aimed to determine the dominance of NDM-producing Enterobacteriaceae at a teaching hospital in Karachi.
Article
Infectious Diseases
Larbi Zakaria Nabti, Farida Sahli, Ahmed Olowo-Okere, Abderrahim Benslama, Abdenassar Harrar, David Lupande-Mwenebitu, Seydina M. Diene, Jean-Marc Rolain
Summary: This study aimed to determine the incidence and molecular mechanisms of carbapenem-resistant Enterobacteriaceae in patients from the Setif University Hospital, Algeria. Among the 123 isolates obtained, Klebsiella pneumoniae, Escherichia coli, and Enterobacter cloacae were the most prevalent species. This study reported the first presence of NDM-5 carbapenemase enzyme in a clinical E. cloacae isolate in Algeria.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Medicine, General & Internal
Raouaa Maaroufi, Olfa Dziri, Linda Hadjadj, Seydina M. Diene, Jean-Marc Rolain, Chedly Chouchani
Summary: This study investigated the presence of Gram-negative bacteria in a hospital environment in Northwestern Tunisia, identifying various resistant strains and characterizing the resistance genes involved. Carbapenem resistance was detected in several isolates, with different resistance genes such as bla(NDM-1) and bla(CTX-M) being identified. Whole-genome sequencing of a P. aeruginosa strain revealed the presence of bla(OXA-494) and other resistance genes. This new epidemiological data highlights the need for new surveillance strategies and strict hygiene rules to control the spread of multidrug-resistant bacteria.
Article
Immunology
Chongmei Tian, Jianqin Song, Lingzhi Ren, Delian Huang, Siwei Wang, Liping Fu, Yaping Zhao, Yongfeng Bai, Xueyu Fan, Tianhong Ma, Junjie Ying
Summary: The isolation of a carbapenem-resistant A. johnsonii AYTCM strain from sputum in China in 2018 is reported. The strain co-produced the NDM-1, OXA-58, and PER 1 enzymes, and exhibited resistance to multiple antibiotics. Whole-genome sequencing revealed the presence of 11 plasmids, with bla(OXA-58) and bla(PER-1) genes located on the pAYTCM-1 plasmid. The study highlights the importance of early surveillance to prevent the spread of high-risk clones in healthcare settings. Rating: 7
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Souad Loqman, Nabila Soraa, Seydina M. Diene, Jean-Marc Rolain
Summary: The study investigated the occurrence of carbapenem-resistant Enterobacteriaceae (CRE) among hospitalized patients in Mohamed VI University Hospital in Marrakech, Morocco. It found various bacterial strains resistant to carbapenems, with Klebsiella pneumoniae being the most common species. The study also identified several carbapenemase-producing isolates, with blaOXA-48 genes being documented for the first time in Citrobacter freundii and Citrobacter braakii in Morocco.
Article
Infectious Diseases
Amel Abderrahim, Nassima Djahmi, Lotfi Loucif, Sabrina Nedjai, Widad Chelaghma, Djamila Gameci-Kirane, Mazouz Dekhil, Jean-Philippe Lavigne, Alix Pantel
Summary: Multidrug-resistant Enterobacterales, particularly carbapenemase producers, are a growing problem in Algeria. This study investigated the molecular characteristics of carbapenem-resistant Enterobacterales isolates in Eastern Algeria, revealing the prevalence of carbapenemase-encoding genes such as bla(OXA-48). Clonal expansions of K. pneumoniae and E. coli were observed, and IncM plasmids were found to be predominant. The global dissemination of OXA-48-producing Enterobacterales in Algerian hospitals and the presence of NDM-1-producing E. coli in community settings highlight the importance of investigation and control measures.
Article
Infectious Diseases
Han Wang, Xuyong Li, Bao-Tao Liu
Summary: The study identified horses in an equestrian club in Qingdao, China carrying NDM-5 and KPC-2 producing bacteria, with plasmids capable of transferring between different bacterial species. The potential transmission risk of these CRE highlights the need for further monitoring of CRE prevalence among equestrian clubs nationwide.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Microbiology
Katerina Tsilipounidaki, Zoi Florou, Anargyros Skoulakis, George C. C. Fthenakis, Vivi Miriagou, Efthymia Petinaki
Summary: The present study genetically characterized ten NDM-1 producing Escherichia coli isolates from a hospital in Central Greece between 2017 and 2021. Whole genome sequencing was used to determine the multi-locus sequencing typing (MLST), bla(NDM1)-environment, resistome, and plasmid content. The isolates showed a diverse range of sequence types and carried multiple resistance genes to beta-lactams and other classes of antibiotics. The analysis also revealed the presence of various plasmids, indicating continuous horizontal gene transfer among the isolates.
Article
Microbiology
Xiaolin Zhu, Yaru Zhang, Zhangqi Shen, Lining Xia, Jinquan Wang, Li Zhao, Ke Wang, Wenhui Wang, Zhihui Hao, Zhihai Liu
Summary: This study investigated the prevalence of NDM-1-producing multidrug-resistant Proteus mirabilis strains in commercial broilers in China. The results showed that these isolates were resistant to multiple antibiotics, with some carrying NDM-1 genes on transferable plasmids. The genomic analysis indicated diverse genetic backgrounds among the isolates, suggesting a potential threat to the environment and public health.
