Article
Microbiology
Yuan Liu, Ye Lin, Ning Guan, Yuting Song, Youguo Li, Xianan Xie
Summary: In this study, a deletion mutant and functional complementary strain of the ADP-L-glycerol-D-mannoheptosyl-6-exoisomerase (rfaD) gene in Mesorhizobium huakuii 7653R were constructed. The results showed that the deletion of rfaD affected the synthesis of lipopolysaccharide (LPS) and increased sensitivity to abiotic stress. The rfaD gene was mainly expressed in the infection zone of mature nodules, and the rfaD mutant strain exhibited delayed nodulation and loss of symbiotic nitrogen fixation ability when interacting with Astragalus sinicus.
Article
Microbiology
Akhilesh Yadav, Raghvendra Pratap Singh, Asha Lata Singh, Major Singh
Summary: The study focuses on describing the molecular mechanism of Mesorhizobium ciceri Ca181 in drought stress tolerance and phosphorus solubilization. The production of gluconic acid is found to be necessary for phosphorus solubilization, while otsA, Auc, and Usp genes are involved in the mechanism of drought stress tolerance in M. ciceri Ca181. Through the development of a mutant library and screening for defective mutants, specific genes related to phosphorus solubilization and drought stress tolerance were identified in Ca181.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Food Science & Technology
Tonghui Liu, Chuqi Feng, Zhaofeng Li, Zhengbiao Gu, Xiaofeng Ban, Yan Hong, Li Cheng, Caiming Li
Summary: This study focused on encapsulating carvacrol with mixed cyclodextrins to improve its stability and release effectiveness. Microencapsulated carvacrol showed better encapsulation efficiency and release performance, offering new possibilities for its applications in the food and pharmaceutical industries.
Article
Plant Sciences
Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara, Shinsuke Shimizu, Koichi Hori, Masako Iwai, Hiroyuki Ohta, Kimitsune Ishizaki, Mie Shimojima
Summary: This study analyzed the impact of membrane lipid remodeling on inorganic phosphate uptake and discovered the crucial role of MpPAH in the growth of Marchantia polymorpha. The mutation of MpPAH affects plant growth by suppressing the synthesis of plastid glycolipids through the endoplasmic reticulum pathway.
Article
Plant Sciences
Maximilian M. F. F. Funfgeld, Wei Wang, Hirofumi Ishihara, Stephanie Arrivault, Regina Feil, Alison M. Smith, Mark Stitt, John E. Lunn, Totte Niittyla
Summary: Through re-examination of experimental data, the study shows that starch synthesis in leaves primarily relies on ADPG pyrophosphorylase in chloroplasts, while sucrose synthase in the cytosol makes no substantial contribution to transitory starch synthesis in Arabidopsis leaves. This finding resolves a long-standing controversy in photosynthetic metabolism.
Article
Biochemistry & Molecular Biology
Joan Patrick, Pontus Pettersson, Lena Maler
Summary: WaaG is a glycosyltransferase involved in bacterial cell wall synthesis. The study used 5-fluoro-Trp labelling and NMR techniques to investigate the dynamic interactions of WaaG with lipids and the donor substrate. The findings provide important insights into the function and structure of WaaG in a biologically relevant environment.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Entomology
Jia Wang, Huan Fan, Ying Li, Tong-Fang Zhang, Ying-Hong Liu
Summary: In this study, three TPP genes from Bactrocera minax were identified and characterized. The different functions of these genes in trehalose synthesis and metamorphosis were revealed. Suppression of these genes led to increased mortality and malformation rate in B. minax, suggesting their potential as insecticidal targets.
Article
Thermodynamics
Piyush Sharma, O. P. Pandey
Summary: The formation of nanolaminated Cr2AlC MAX phase by using solid-state synthesis route was investigated, confirming the formation of Cr2AlC and estimating the kinetic triplets involved in the synthesis process. The results indicated that F2 (second-order) reaction mechanism dominates the formation of Cr2AlC MAX phase.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Article
Engineering, Chemical
Yin Li, Li Wang, Keyu Zhang, Yaochun Yao, Lingxin Kong
Summary: LiFePO4 is a promising cathode material for lithium ion batteries due to its nontoxicity, high specific capacity, good safety characteristics, and low cost. The use of phytic acid as a new phosphorus source for synthesis has shown significant effects on morphology and electrochemical performance. After carbon coating, LiFePO4 exhibits outstanding initial discharge capacity and high temperature behavior, which is crucial for the controllable synthesis and improvement of its electrochemical characteristics.
ADVANCED POWDER TECHNOLOGY
(2021)
Article
Environmental Sciences
Diana Del Rosario-Santana, Rafael Robaina, Pilar Garcia-Jimenez
Summary: In this study, changes in cell wall sulfated galactans in Grateloupia imbricata were monitored after rapid induction of cystocarp maturation and carpospore development by the volatile hormone methyl jasmonate (MEJA). The synthesis of sulfated galactans was followed by gene expression analysis, and structural changes were analyzed using FTIR spectra. The results showed downregulation of key genes involved in cell wall synthesis, and alterations in the FTIR spectrum confirmed the induction of haploid spores by MEJA.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Plant Sciences
X. -y. Huang, Y. -y. LI, T. -j. Zhao, W. -y. Liu, Y. -n. Feng, L. Wang, Y. -c. Ma, X. -f. Lin
Summary: This study identified CkTPS and CkTPP genes from Caragana korshinskii, which play important roles in synthesizing trehalose and enhancing stress resistance in plants. The expressions of CkTPS and CkTPP genes were upregulated under drought stress, and their promoters were strongly induced by various signaling pathways. Introduction of CkTPS and CkTPP into Arabidopsis resulted in improved drought tolerance, higher germination rate, and better growth compared to wild-type plants. The findings provide a basis for understanding the function and regulatory mechanism of CkTPS and CkTPP, as well as the potential application of genetic engineering for enhancing drought tolerance in crops.
BIOLOGIA PLANTARUM
(2022)
Article
Engineering, Environmental
Jiayu Shen, Haijun Chen, Nan Xu, Yunpeng Liu, Wu Sun, Xinyue Ma, Ruiyang Sun, Yuxi Gao, Jiating Zhao
Summary: The migration of nano zerovalent iron (nZVI) materials to contaminated sites is limited, hindering the in situ remediation of hexavalent chromium (Cr(VI)) pollution. In this study, the transportability of Mo/nZVI@GT materials was improved using green tea extracts and molybdate. The Mo/nZVI@GT showed pronounced size-selective retention in porous media and enhanced Cr(VI) reduction in the presence of phosphate. Tea polyphenols promoted the production of Mo(IV) species, enhancing the conversion of Cr(VI) to Cr2O3. The study elucidated the mechanisms of Cr(VI) reduction and removal and highlighted the potential application of Mo/nZVI@GT composites in efficient in situ remediation of Cr(VI)-contaminated groundwater and soil.
CHEMICAL ENGINEERING JOURNAL
(2024)
Article
Chemistry, Multidisciplinary
Francisco Ruiz-Jorge, Almudena Benitez, M. Belen Garcia-Jarana, Jezabel Sanchez-Oneto, Juan R. Portela, Enrique J. Martinez de la Ossa
Summary: Lithium-ion batteries (LIBs) have gained much interest due to increasing energy demand and climate change. Cathode materials, particularly LiFePO4, are crucial for LIBs, but efforts are still needed to improve purity and reduce operating costs.
Article
Chemistry, Inorganic & Nuclear
Qi Zhang, Weiguo Fang, Liuliu Wang, Guixian Li, Yu Zhao
Summary: Adding an appropriate amount of water is beneficial for forming pore structure in VPO materials prepared in DES, while excessive water can destroy the pore structure and morphology. The crystallite size of VPO materials increases with the additional water content, and the phase changes from amorphous to crystalline.
JOURNAL OF SOLID STATE CHEMISTRY
(2021)
Article
Endocrinology & Metabolism
Jong Shin, Hannan Mir, Maaz A. A. Khurram, Kenji M. M. Fujihara, Brian D. D. Dynlacht, Timothy J. J. Cardozo, Richard Possemato
Summary: This study uncovers the mechanism by which human de novo pyrimidine biosynthesis is regulated during the cell cycle. The researchers identify carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD) as the key enzymes regulated in the cell cycle, and demonstrate that allostery plays a major role in controlling CAD activity. They also find evidence of Animalia-specific loops in the CAD allosteric domain, which are involved in sensing and binding of uridine 5'-triphosphate, an allosteric inhibitor of CAD. Through structural analysis, a critical signal transmission loop is identified in regulating the formation of a substrate channel and controlling CAD activity.
Article
Biochemistry & Molecular Biology
Meike Siebers, Mathias Brands, Vera Wewer, Yanjiao Duan, Georg Hoelzl, Peter Doermann
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2016)
Article
Chemistry, Applied
Manel Zitouni, Vera Wewer, Peter Doermann, Chedly Abdelly, Nabil Ben Youssef
Article
Biochemistry & Molecular Biology
Christopher Stoffels, Mhmd Oumari, Aris Perrou, Andreas Termath, Waldemar Schlundt, Hans-Guenther Schmalz, Mathias Schaefer, Vera Wewer, Sabine Metzger, Edgar Schoemig, Dirk Gruendemann
FREE RADICAL BIOLOGY AND MEDICINE
(2017)
Article
Parasitology
Vera Wewer, Benjamin L. Makepeace, Vincent N. Tanya, Helga Peisker, Kenneth Pfarr, Achim Hoerauf, Peter Doermann
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Simona Eicke, David Seung, Barbara Egli, Emanuel A. Devers, Sebastian Streb
METABOLIC ENGINEERING
(2017)
Article
Plant Sciences
Armando Bravo, Mathias Brands, Vera Wewer, Peter Doermann, Maria J. Harrison
Article
Multidisciplinary Sciences
Anita Loeschcke, Dennis Dienst, Vera Wewer, Jennifer Hage-Huelsmann, Maximilian Dietsch, Sarah Kranz-Finger, Vanessa Hueren, Sabine Metzger, Vlada B. Urlacher, Tamara Gigolashvili, Stanislav Kopriva, Ilka M. Axmann, Thomas Drepper, Karl-Erich Jaeger
Article
Plant Sciences
Mathias Brands, Vera Wewer, Andreas Keymer, Caroline Gutjahr, Peter Doermann
Article
Biochemistry & Molecular Biology
Florian Brioudes, Florence Jay, Alexis Sarazin, Thomas Grentzinger, Emanuel A. Devers, Olivier Voinnet
Summary: In this study, an artificial miRNA-based system was used to explore the mechanisms of inter-cellular miRNA movement in plants, revealing the role of HST in regulating the movement of processed miRNAs between cells and tissues. The findings suggest that HST may play a crucial role in controlling the movement of miRNAs within plants.
Article
Parasitology
Vera Wewer, Helga Peisker, Katharina Gutbrod, Mazen Al-Bahra, Dirk Menche, Ngongeh Glory Amambo, Fanny F. Fombad, Abdel Jelil Njouendou, Kenneth Pfarr, Samuel Wanji, Achim Hoerauf, Peter Doermann
Summary: The study screened urine samples from individuals in Cameroon infected with O. volvulus, Loa loa, Mansonella perstans, or a combination. Elevated levels of NATOG and cinnamoylglycine were found in O. volvulus-infected individuals, suggesting a potential for using a combination of urine metabolites for onchocerciasis assessment at a population level.
PARASITES & VECTORS
(2021)
Article
Plant Sciences
Sandra E. Zimmermann, Ruben M. Benstein, Maria Flores-Tornero, Samira Blau, Armand D. Anoman, Sara Rosa-Tellez, Silke C. Gerlich, Mohamed A. Salem, Saleh Alseekh, Stanislav Kopriva, Vera Wewer, Ulf-Ingo Fluegge, Richard P. Jacoby, Alisdair R. Fernie, Patrick Giavalisco, Roc Ros, Stephan Krueger
Summary: The study reveals the crucial role of the phosphorylated pathway of serine biosynthesis (PPSB) in plant growth and metabolism, with deficiency in PPSB leading to nitrogen assimilation induction.
Article
Plant Sciences
Emanuel A. Devers, Christopher A. Brosnan, Alexis Sarazin, Gregory Schott, Peiqi Lim, Satu Lehesranta, Yrjo Helariutta, Olivier Voinnet
Summary: The mechanism of RNA interference (RNAi) in plants is not well understood, especially the propagation and selectivity of siRNAs. Through research, it has been found that RNAi in plants is propagated through plasmodesmata (PDs) and involves specific ARGONAUTE (AGO) proteins. Stress enhancement also plays a role in the translocation and regulation of endogenous siRNAs.
Article
Plant Sciences
Emanuel A. Devers, Christopher A. Brosnan, Alexis Sarazin, Daniele Albertini, Andrea C. Amsler, Florian Brioudes, Pauline E. Jullien, Peiqi Lim, Gregory Schott, Olivier Voinnet
Article
Biology
Andreas Keymer, Priya Pimprikar, Vera Wewer, Claudia Huber, Mathias Brands, Simone L. Bucerius, Pierre-Marc Delaux, Verena Klingl, Edda von Roepenack-Lahaye, Trevor L. Wang, Wolfgang Eisenreich, Peter Doermann, Martin Parniske, Caroline Gutjahr
Article
Biotechnology & Applied Microbiology
Jennifer Hage-Huelsmann, Sabine Metzger, Vera Wewer, Felix Buechel, Katrin Troost, Stephan Thies, Anita Loeschcke, Karl-Erich Jaeger, Thomas Drepper
JOURNAL OF BIOTECHNOLOGY
(2019)