Article
Gastroenterology & Hepatology
Guangli Wang, Chan Fan, Hui Wang, Chengyi Jia, Xiaoting Li, Jianru Yang, Tao Zhang, Song Gao, Xun Min, Jian Huang
Summary: This study reveals a novel regulatory role of the FHA domain protein TagH in controlling the hemolytic activity of Vibrio cholerae, in addition to regulating the T6SS. TagH negatively regulates HlyA expression at the transcriptional and post-translational levels, and the phosphopeptide binding sites of the FHA domain play a crucial role in the regulation of hemolytic activity. Deletion of tagH enhances the intestinal pathogenicity and extraintestinal invasion ability of V. cholerae, which mostly rely on the expression of HlyA.
Article
Multidisciplinary Sciences
Benjamin Kostiuk, Francis J. Santoriello, Laura Diaz-Satizabal, Fabiana Bisaro, Kyung-Jo Lee, Anna N. Dhody, Daniele Provenzano, Daniel Unterweger, Stefan Pukatzki
Summary: It was found that classical Vibrio cholerae underwent sequential mutations in T6SS genetic determinants, making them disadvantaged in competition with the 7th pandemic El Tor biotype strains.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Siu Lung Ng, Sophia Kammann, Gabi Steinbach, Tobias Hoffmann, Peter J. Yunker, Brian K. Hammer
Summary: Organisms sense external cues and use transcription factors to regulate gene expression and adapt to new niches. This study identifies the regulatory element of the major type VI locus in Vibrio cholerae and discovers a single nucleotide polymorphism that controls the killing activity of the type VI secretion system. This finding highlights the role of noncoding DNA in pathogen adaptation.
Article
Multidisciplinary Sciences
Milena Jaskolska, David W. Adams, Melanie Blokesch
Summary: Horizontal gene transfer is a key driver of bacterial evolution, facilitated by mobile genetic elements such as plasmids and bacteriophages. This study identifies two conserved plasmid defence systems in Vibrio cholerae, responsible for the ongoing cholera pandemic. These systems rapidly eliminate small plasmids and defend against bacteriophage infection. Additionally, they increase the burden of large conjugative plasmids, leading to a fitness disadvantage for plasmid-carrying cells. These findings explain the rarity of plasmids in pandemic strains and have implications for understanding antibiotic resistance plasmid dissemination and the evolution of pandemic V. cholerae.
Article
Biochemistry & Molecular Biology
Junxiang Yan, Qian Liu, Xinke Xue, Jinghao Li, Yuehua Li, Yingying Su, Boyang Cao
Summary: This study reveals that the VC1795 gene plays a key role in the intestinal colonization and pathogenicity of Vibrio cholerae, and it regulates the expression of its downstream gene, VC1794, and the toxin-coregulated pilus (TCP) cluster. These findings provide insights into the regulatory mechanisms of VC1795 in bacterial pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Minh Nguyen, Tzu-Hui Wu, Katie J. Danielson, Nabeel M. Khan, John Zhijia Zhang, Lisa Craig
Summary: Many bacteria have dynamic filaments called Type IV pili (T4P) that play various roles in colonization and dissemination. A specific segment in the TcpF protein functions as an export signal (ES) recognized by the Vibrio cholerae toxin-coregulated pilus (TCP). This ES is proteolyzed from TcpF during secretion and is capable of mediating the export of Neisseria gonorrhoeae FbpA by V. cholerae. Specificity is achieved through the interaction between the ES and TcpB, a minor pilin protein. These findings provide insights into the mechanism of TcpF delivery across the outer membrane.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Sebastien Breurec, Thierry Franck, Elisabeth Njamkepo, Jean-Robert Mbecko, Jean Rauzier, Hugues Sanke-Waigana, Guyguy Kamwiziku, Renaud Piarroux, Marie-Laure Quilici, Francois-Xavier Weill
Summary: Four cholera outbreaks were reported in the Central African Republic during 1997-2016, caused by Vibrio cholerae O1 serotype Inaba from 3 seventh pandemic El Tor sublineages originating from West Africa or the African Great Lakes Region.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Immunology
Xiaoshu Liu, Jingjing Pan, He Gao, Yu Han, Anran Zhang, Yuanming Huang, Ping Liu, Biao Kan, Weili Liang
Summary: In this study, the researchers found that the CqsA/LuxS-HapR quorum sensing system regulates the VflT6SS2 in Vibrio fluvialis through the regulator proteins LuxO and HapR. HapR is crucial for the promoter activity of VflT6SS2.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Immunology
Nabila Benamrouche, Chafika Belkader, Elisabeth Njamkepo, Sarah Sihem Zemam, Soraya Sadat, Karima Saighi, Dalila Torkia Boutabba, Faiza Mechouet, Rym Benhadj-Slimani, Fatma-Zohra Zmit, Jean Rauzier, Farid Kias, Souad Zouagui, Corinne Ruckly, Mohamed Yousfi, Amel Zertal, Ramdane Chouikrat, Marie-Laure Quilici, Francois-Xavier Weill
Summary: After a lull of more than 20 years, Algeria experienced a cholera outbreak in 2018 with 291 suspected cases. The outbreak strains were identified as Vibrio cholerae O1 serotype Ogawa from the seventh pandemic El Tor sublineage AFR14, indicating a new introduction of cholera into Africa from South Asia.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Microbiology
Robert C. Kauffman, Oluwaseyi Adekunle, Hanyi Yu, Alice Cho, Lindsay E. Nyhoff, Meagan Kelly, Jason B. Harris, Taufiqur Rahman Bhuiyan, Firdausi Oadri, Stephen B. Calderwood, Richelle C. Charles, Edward T. Ryan, Jun Kong, Jens Wrammert
Summary: The properties and protective mechanisms of functionally relevant antibodies against Vibrio cholerae have been investigated, showing that affinity, immunoglobulin isotype, and subclass do not directly dictate functional potency. Importantly, low-affinity variants displayed comparable agglutination and motility inhibition properties to high-affinity antibodies, suggesting that the way the antibody binds to the O-antigen may be critical to function.
Article
Biochemistry & Molecular Biology
Landon J. Getz, Justin M. Brown, Lauren Sobot, Alexandra Chow, Jastina Mahendrarajah, Nikhil A. Thomas
Summary: Pathogenic Vibrio species cause 3-5 million life-threatening human infections annually. The winged helix-turn-helix (wHTH) transcriptional regulator HlyU positively regulates the expression of bacterial hemolysin and toxin genes, while histone-like nucleoid structural protein (H-NS) silences their expression. HlyU is required for virulence gene expression associated with type 3 Secretion System-1 (T3SS1) in Vibrio parahaemolyticus, although its mechanism of action is not understood.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Microbiology
Jennifer K. Teschler, Eva Jimenez-Siebert, Hannah Jeckel, Praveen K. Singh, Jin Hwan Park, Stefan Pukatzki, Carey D. Nadell, Knut Drescher, Fitnat H. Yildiz
Summary: This study demonstrates that the Vibrio cholerae type six secretion system (T6SS) can actively kill prey strains within the interior of biofilm populations with substantial impact on population dynamics. We additionally show that the response regulator VxrB contributes to both T6SS killing and protection from T6SS killing within biofilms.
Article
Microbiology
Yun Luo, Michael Payne, Sandeep Kaur, Sophie Octavia, Jianmin Jiang, Ruiting Lan
Summary: This study sequenced and compared non-pandemic Vibrio cholerae O1 isolates from Zhejiang, China with publicly available non-pandemic O1 genomes. The isolates were classified into three lineages, with one lineage sharing the most recent common ancestor with the pandemic clones. Each lineage had unique properties contributing to their disease-causing capacity. The findings provide new insights into the evolution of V. cholerae O1 for cholera prevention and control.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Jennifer K. Teschler, Carey D. Nadell, Knut Drescher, Fitnat H. Yildiz
Summary: Biofilms are a growth mode in which microbial communities are structured and embedded in a polymeric extracellular matrix. In this study, the biofilm formation of Vibrio cholerae is focused and the current understanding of its formation, including attachment, matrix components, dynamics, regulation, and dispersal, is summarized. The decision to form or disperse from biofilms is regulated by a complex network that integrates various environmental inputs.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Article
Microbiology
Stephanie Smith, Fernanda Salvato, Aditi Garikipati, Manuel Kleiner, Alecia N. Septer
Summary: Bacteria utilize diverse strategies like T6SS for survival competition, with essential structural proteins and transcriptional regulators controlling expression. These mechanisms ensure energy requirements are met for building costly T6SS weapons, providing a direct link between energy levels and T6SS activation.
JOURNAL OF BACTERIOLOGY
(2021)
Editorial Material
Multidisciplinary Sciences
Daniel Unterweger, Ashleigh S. Griffin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Editorial Material
Biochemistry & Molecular Biology
Daniel Unterweger, Benjamin Kostiuk, Stefan Pukatzki
TRENDS IN MICROBIOLOGY
(2017)
Article
Multidisciplinary Sciences
Paul C. Kirchberger, Daniel Unterweger, Daniele Provenzano, Stefan Pukatzki, Yan Boucher
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
William P. J. Smith, Maj Brodmann, Daniel Unterweger, Yohan Davit, Laurie E. Comstock, Marek Basler, Kevin R. Foster
NATURE COMMUNICATIONS
(2020)
Review
Immunology
Matthias Merker, Leif Tueffers, Marie Vallier, Espen E. Groth, Lindsay Sonnenkalb, Daniel Unterweger, John F. Baines, Stefan Niemann, Hinrich Schulenburg
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Francis J. Santoriello, Lina Michel, Daniel Unterweger, Stefan Pukatzki
NATURE COMMUNICATIONS
(2020)
Article
Microbiology
Jeffrey W. Turner, Jorge Duran-Gonzalez, David A. Laughlin, Daniel Unterweger, David Silva, Boris Ermolinsky, Stefan Pukatzki, Daniele Provenzano
Summary: This study presents draft genome sequences of 13 non-O1/O139 V. cholerae strains isolated from the Rio Grande Delta in Texas, which will facilitate future analyses of environmental serovars.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Multidisciplinary Sciences
Benjamin Kostiuk, Francis J. Santoriello, Laura Diaz-Satizabal, Fabiana Bisaro, Kyung-Jo Lee, Anna N. Dhody, Daniele Provenzano, Daniel Unterweger, Stefan Pukatzki
Summary: It was found that classical Vibrio cholerae underwent sequential mutations in T6SS genetic determinants, making them disadvantaged in competition with the 7th pandemic El Tor biotype strains.
NATURE COMMUNICATIONS
(2021)
Review
Microbiology
Rahul Unni, Katherine L. Pintor, Andreas Diepold, Daniel Unterweger
Summary: In this review, the authors discuss the presence and absence of the type VI secretion apparatus among bacteria and speculate on the dynamic process of gaining and losing the secretion system to better understand its spread and distribution across the microbial world.
Article
Microbiology
Hanna Fokt, Rahul Unni, Urska Repnik, Ruth A. Schmitz, Marc Bramkamp, John F. Baines, Daniel Unterweger
Summary: Two novel bacterial strains, KH365_2(T) and KH569_7, were isolated from the cecum contents of wild-derived house mice. These strains were characterized as Gram-negative, rod-shaped, strictly anaerobic, and non-motile. Phylogenetic analysis revealed that they belonged to a new species of the genus Bacteroides, named Bacteroides muris sp. nov. The newly isolated strains showed similar morphological and biochemical characteristics to known Bacteroides strains, but their average nucleotide identity and DNA-DNA hybridization values distinguished them from described members of the genus Bacteroides.
ARCHIVES OF MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Benjamin Kostiuk, Daniel Unterweger, Daniele Provenzano, Stefan Pukatzki
INTERNATIONAL MICROBIOLOGY
(2017)