Article
Biochemistry & Molecular Biology
Marta Woroszylo, Daria Ciecholewska-Jusko, Adam Junka, Radoslaw Drozd, Marcin Wardach, Pawel Migdal, Patrycja Szymczyk-Ziolkowska, Daniel Styburski, Karol Fijalkowski
Summary: The combination of rotating magnetic field (RMF) with beta-lactam antibiotics shows promising results in enhancing the antibacterial effects against methicillin-resistant Staphylococcus aureus (MRSA) by causing morphological alterations in bacterial cells. This study provides potential new treatment options for MRSA infections by utilizing the disruptive effect of RMF on bacterial cell walls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jenna E. Beam, Sophie Maiocchi, Ana Cartaya, Sarah E. Rowe, Edward S. M. Bahnson, Brian P. Conlon
Summary: Interactions between Staphylococcus aureus and the host immune system can significantly affect antibiotic efficacy. Modulating the immune response may improve antibiotic efficacy and infection outcome.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Suat Sari, Suna Sabuncuoglu, Ebru Kocak Aslan, Ahmet Avci, Didem Kart, Zeynep Ozdemir, M. Fahir Acar, Burcu Sayoglu, M. Abdullah Alagoz, Arzu Karakurt, Sevim Dalkara
Summary: This study identified a series of naphthalene-bearing azoles as potent antibacterial agents against Gram-positive bacteria. These compounds showed high activity and safety against bacterial strains. Computational and molecular docking studies suggested that the mechanism of action might involve the inhibition of bacterial flavohemoglobin.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Microbiology
Tongtong Fu, Zheng Fan, Yujie Li, Zhoufei Li, Bing Du, Shiyu Liu, Xiaohu Cui, Rui Zhang, Hanqing Zhao, Yanling Feng, Guanhua Xue, Jinghua Cui, Chao Yan, Lin Gan, Junxia Feng, Ziying Xu, Zihui Yu, Ziyan Tian, Zanbo Ding, Jinfeng Chen, Yujie Chen, Jing Yuan
Summary: This study found that deletion of the ArcR gene in clinical isolates of Staphylococcus aureus can reduce the strain's tolerance to oxidative stress, leading to increased sensitivity to fluoroquinolone antibiotics. This result reveals the significant role of ArcR in bacterial tolerance to oxidative stress and subsequently to fluoroquinolones antibiotics.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Maureen Kleinhenz, Pavani Beesetty, Ching Yang, Zhaotao Li, Christopher P. Montgomery
Summary: Recurrent Staphylococcus aureus infections are common, but no approved vaccine is available. Antibiotic therapy resolves infection but fails to elicit protective immunity. Combined vaccination and antibiotics can treat infection and generate durable protective immunity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Frederic Peyrusson, Tiep Khac Nguyen, Tome Najdovski, Francoise Van Bambeke
Summary: This study investigates the effect of reactive oxygen species (ROS) on the dormancy depth of intracellular S. aureus persisters in host cells. It is found that host ROS can induce variable dormant states in persisters and the level of oxidative stress determines the capacity for resuscitation. The results highlight the importance of understanding the interplay between ROS, ATP depletion, and dormancy state in S. aureus infections.
MICROBIOLOGY SPECTRUM
(2022)
Review
Multidisciplinary Sciences
Juan Chen, Huyue Zhou, Jingbin Huang, Rong Zhang, Xiancai Rao
Summary: This review explores the impact of sub-MICs of antibiotics on the virulence of Staphylococcus aureus, focusing on various aspects such as changes in cell morphology, virulence factor expression, bacterial adherence and invasion, biofilm formation, and small-colony variant production. Antibiotics at sub-MIC levels can alter bacterial virulence in S. aureus, leading to deformed cells, abnormal immune responses, and changes in disease development. The study also addresses the mechanisms underlying these antibiotic-induced alterations in virulence.
JOURNAL OF ADVANCED RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Yiqing Zou, Xiaonan Chen, Yingying Sun, Pengyu Li, Mao Xu, Pengchao Fang, Shuqi Zhang, Gang Yuan, Xin Deng, Haiyan Hu
Summary: In this study, fucoidan-coated nanoparticles were developed to target and destroy Helicobacter pylori biofilms, as well as kill the bacteria inside host cells. These nanoparticles not only improved bacterial eradication but also alleviated oxidative stress and reduced gastric mucosal damage.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Infectious Diseases
Danilo Buonsenso, Martina Giaimo, Davide Pata, Alessia Rizzi, Barbara Fiori, Teresa Spanu, Antonio Ruggiero, Giorgio Attina, Marco Piastra, Orazio Genovese, Giovanni Vento, Simonetta Costa, Eloisa Tiberi, Maurizio Sanguinetti, Piero Valentini
Summary: The study focuses on Staphylococcus aureus infection in children, analyzing its antibiotic resistance profile and therapeutic management. The results show high resistance rates to cephalosporins, aminoglycosides, and fluoroquinolones, but no resistance to glycopeptides. Age, presence of devices, and comorbidities are identified as major risk factors associated with antibiotic resistance. Understanding the resistance patterns helps to target therapy effectively while following the principles of antibiotic stewardship is crucial in limiting the spread of antibiotic resistance.
Review
Biochemistry & Molecular Biology
Erik T. Nesson, Susan A. McDowell
Summary: An emerging therapeutic approach in the treatment of infectious disease is to augment the host response through repurposing of well-tolerated, non-antibiotic, host-directed therapeutics. However, the therapeutic benefit of statin use in complex infection settings is still controversial, and potential biases in observational studies may lead to an overestimation of the benefits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Greg A. Somerville, Allison A. Parrett, Joseph M. Reed, Stewart G. Gardner, Martha Morton, Robert Powers
Summary: The metabolic adaptation of Staphylococcus aureus in a complex environment is crucial for its antibiotic resistance.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Food Science & Technology
Mengyuan Qi, Ying Liu, Susu Shi, Youhua Xian, Qingyan Liu, Haiyang Yan, Yan Zhang, Yuan Yuan
Summary: This study investigated the mechanism of high voltage prick electrostatic field (HVPEF) against Staphylococcus aureus (S. aureus) from the perspective of ROS-mediated oxidative stress. The results showed that HVPEF increased ROS levels and induced cell damage, including oxidative damage to DNA and proteins and bacterial apoptosis. This study provides a better understanding of the antibacterial mechanism of HVPEF.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Josefien W. Hommes, Bas G. J. Surewaard
Summary: MRSA infections pose a global health threat and have a high mortality rate. In addition to being an extracellular pathogen, MRSA can also survive and replicate intracellularly, using the antimicrobial environment of phagocytes to evade immune cells and antibiotics. The intracellular niche of bacteria may contribute to the development of antibiotic tolerance, and there are various new antibacterial strategies targeting this intracellular bacterial niche.
Article
Chemistry, Medicinal
Dingyi Shen, Songpo Duan, Yuhan Hu, Jintong Liang, Youzhi Tang
Summary: This study found that the phloroglucinol derivative TPG and 2-nitrophloroglucinol exhibited potent synergistic antibacterial activity with penicillin or bacitracin against MRSA. The combination disrupted the MRSA cell membrane and inhibited the expression of related proteins, effectively inhibiting MRSA growth.
PHYTOTHERAPY RESEARCH
(2023)
Article
Microbiology
Jinglong Liang, Teng Yi Huang, Yuzhu Mao, Xuejie Li
Summary: The biofilm formation of two genetically diverse Staphylococcus aureus isolates, 10379 and 121940, was evaluated under different concentrations of beta-lactam antibiotics. Despite their genetic differences, sub-inhibitory concentrations of beta-lactam antibiotics promoted biomass and biofilm viability of both isolates.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Chemistry, Inorganic & Nuclear
Jordi Zamarreno Beas, Marco A. M. Videira, Ligia M. Saraiva
Summary: Haem b and sirohaem are two important prosthetic groups in bacterial proteins, synthesized through complex pathways with divergences at the formation of uroporphyrinogen III. The biosynthesis of these tetrapyrroles involves unstable or potentially hazardous intermediates and end-products, requiring tight control of cellular metabolic fluxes.
COORDINATION CHEMISTRY REVIEWS
(2022)
Review
Chemistry, Inorganic & Nuclear
Giorgio Caserta, Lidia Zuccarello, Catarina Barbosa, Celia M. Silveira, Elin Moe, Sagie Katz, Peter Hildebrandt, Ingo Zebger, Smilja Todorovic
Summary: The universe of known biological FeS clusters is expanding with novel and unprecedented structures, including unique coordination and heteronuclear clusters that play versatile physiological roles. Some structures with distorted geometries or atypical ligations exhibit novel functions. Resonance Raman spectroscopy plays a key role in unraveling remarkable features in FeS centers and understanding their unusual structures and functions.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Konstantin Laun, Benjamin R. Duffus, Stefan Wahlefeld, Sagie Katz, Dennis Belger, Peter Hildebrandt, Maria Andrea Mroginski, Silke Leimkuehler, Ingo Zebger
Summary: It is found that the Mo-dependent formate dehydrogenase from Rhodobacter capsulatus can catalyze the oxidation of formate to CO2. Infrared spectroscopy, density functional theory, and inhibition kinetics were used to investigate the interaction between the enzyme and inhibitory molecules. The study reveals potential non-competitive or competitive binding sites for the inhibitors in the secondary coordination sphere of the active site.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Liliana S. O. Silva, Pedro M. Matias, Celia Romao, Ligia M. Saraiva
Summary: Repair of Iron Center proteins (RIC) are a family of di-iron proteins found widely in microbes. This review summarizes the data on microbial RICs, including their gene regulation and contribution to pathogenic bacteria survival. The focus is on Escherichia coli RIC, with extensive biochemical characterization and new structural data featuring a di-manganese site. The networking of protein interactions involving RIC and its proposed physiological role as an iron donor for repairing damaged iron-sulfur centers are also described.
Article
Chemistry, Physical
Tillmann Utesch, Jana Staffa, Sagie Katz, Guiyang Yao, Jacek Kozuch, Peter Hildebrandt
Summary: The article investigates the effect of electrode potential on the transmembrane potential of lipid bilayers immobilized on gold electrodes. Experimental results and molecular dynamics simulations show good agreement, indicating the promising potential of this combined approach in studying potential-dependent processes at biomimetic interfaces.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Chemistry, Physical
Konstantin Laun, Benjamin R. Duffus, Hemant Kumar, Jean-Pierre H. Oudsen, Chara Karafoulidi-Retsou, Armel Tadjoung Waffo, Peter Hildebrandt, Khoa Hoang Ly, Silke Leimkuehler, Sagie Katz, Ingo Zebger
Summary: A minimal light-driven approach was developed to study enzymatic CO2 conversion. This approach allows for in situ characterization of the catalytic reaction and provides a new way to regulate enzyme reactions using light.
Article
Chemistry, Multidisciplinary
Vladimir Pelmenschikov, Delfim Ferreira, Sofia S. Venceslau, Peter Hildebrandt, Ines A. . C. Pereira, Smilja Todorovic, Smilja Todorovic
Summary: In this study, resonance Raman spectroscopy and density functional theory calculations were used to investigate the structural, electronic, and vibrational properties of the noncubane [4Fe-4S] cluster in heterodisulfide reductase (HdrB). Homology modeling was employed to predict the protein environment of the neighboring clusters in DvHdrB. The results showed that the oxidized [4Fe-4S](3+) cluster adopts a closed conformation in the absence of substrate, but transitions to an open structure upon substrate coordination, accompanied by a ligand switch. This study provides valuable insights for future research on enzymes with this unconventional cofactor.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Anna Seck, Salvatore De Bonis, Meike Stelter, Mats Okvist, Muge Senarisoy, Mohammad Rida Hayek, Aline Le Roy, Lydie Martin, Christine Saint-Pierre, Celia M. Silveira, Didier Gasparutto, Smilja Todorovic, Jean-Luc Ravanat, Joanna Timmins
Summary: Bacterial nucleotide excision repair (NER) is a multistep, ATP-dependent process that removes a wide range of chemically and structurally diverse DNA lesions. The study investigated the oligomeric state, DNA-binding abilities, and incision activities of UvrC in Deinococcus radiodurans, and constructed the first model of a complete UvrC. The study provides important insights into the recruitment and activation mechanisms of UvrC during NER.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Physical
Victoria Davis, Nina Heidary, Amandine Guiet, Khoa Hoang Ly, Maximilian Zerball, Claudia Schulz, Norbert Michael, Regine von Klitzing, Peter Hildebrandt, Stefan Frielingsdorf, Oliver Lenz, Ingo Zebger, Anna Fischer
Summary: The oxygen tolerance of membrane-bound [NiFe] hydrogenase (MBH) from Cupriavidus necator makes it an attractive electrocatalyst for hydrogen energy conversion devices. By immobilizing MBH with affinity tags on a tin-rich indium tin oxide (ITOTR) electrode, hydrogen oxidation and an unusually high proton reduction current were observed, suggesting bidirectionality in unidirectional [NiFe] hydrogenases. The favorable enzyme-semiconductor interactions contribute to this behavior.
Review
Biochemistry & Molecular Biology
Peter Hildebrandt
Summary: This review summarizes how resonance Raman and IR spectroscopy contributed to the understanding of the structure, dynamics, and reaction mechanism of phytochromes. It also discusses the challenges faced in experimental and theoretical research and describes strategies to overcome them.
Article
Biochemistry & Molecular Biology
Filipa Calisto, Smilja Todorovic, Ricardo O. Louro, Manuela M. Pereira
Summary: This study used nanodiscs and liposomes technology to investigate the ACIII activity in membrane-mimicking systems of Rhodothermus marinus, and also studied the interaction of ACIII with menaquinone, HiPIP, cytochrome c, and caa3 oxygen reductase.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Chemistry, Multidisciplinary
Tarali Devi, Kuheli Dutta, Jennifer Deutscher, Stefan Mebs, Uwe Kuhlmann, Michael Haumann, Beatrice Cula, Holger Dau, Peter Hildebrandt, Kallol Ray
Summary: This study emphasizes the importance of subtle electronic changes and secondary interactions in the stability of biologically relevant metal-dioxygen intermediates. It also shows that the role of the chloride ligand in stabilizing the Fe-III-(OOBu)-Bu-t moiety can extend to other anions, including the thiolate ligand.
Article
Chemistry, Multidisciplinary
Elin Moe, Celia M. Silveira, Lidia Zuccarello, Filipe Rollo, Meike Stelter, Salvatore De Bonis, Catharina Kulka-Peschke, Sagie Katz, Peter Hildebrandt, Ingo Zebger, Joanna Timmins, Smilja Todorovic
Summary: This study provides the first comparative biophysical analysis of full-length and N-terminally truncated hNTH1, revealing distinct properties of the two forms. It also highlights the importance of the N-terminal domain for DNA binding during the onset of damage recognition.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Maria Fernandez Lopez, Margarethe Dahl, Francisco Velazquez Escobar, Hernan Ruy Bonomi, Anastasia Kraskov, Norbert Michael, Maria Andrea Mroginski, Patrick Scheerer, Peter Hildebrandt
Summary: This study investigates the structure and mechanistic properties of different types of phytochromes in bacteria using resonance Raman and IR difference spectroscopy, and reveals subtle differences in the photoinduced conversions and proton transfer processes.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)