Review
Microbiology
Xiaotian Zhou, Anna Roujeinikova
Summary: Recent studies have shown the presence of additional periplasmic structures in polar flagellar motors, such as disk or cage-like morphologies, which are crucial for motor assembly and function. These structures enable polar flagellar motors to sustain higher torque and achieve faster swimming speeds compared to peritrichous bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Kohei Yamazaki, Takashige Kashimoto, Ayuha Niwano, Moeko Yamasaki, Mayu Nomura, Yukihiro Akeda, Shunji Ueno
Summary: The study demonstrates that motility in Aeromonas hydrophila is crucial for rapid expansion of necrosis and progression to death within a short time period, despite not being essential for proliferation in the host. A non-motile strain of the bacterium can reduce the extent of necrosis and prolong survival times in infected mice.
Article
Microbiology
Gabriel Forn-Cuni, Kelly M. Fulton, Jeffrey C. Smith, Susan M. Twine, Elena Mendoza-Barbera, Juan M. Tomas, Susana Merino
Summary: This study demonstrates that genes involved in polar flagella glycosylation in mesophilic Aeromonas strains are clustered in highly polymorphic genomic islands. Bioinformatic analysis identified three types of polar flagella glycosylation islands, corresponding to modifications with single monosaccharide and heterogeneous glycans. Group II strains contain a common glycosyltransferase, fgi-1, downstream of luxC, which transfers the first sugar of heterogeneous glycans to the pseudaminic acid derivative linked to flagellins.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Michael D. Manson
Summary: Rotation is a common phenomenon in our daily lives, and it was once believed to be a uniquely human invention. However, research has shown that rotating nanomachines have independently evolved at least three times and play a crucial role in various cellular processes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Xueshu Zhang, Bingyan Sun, Yulin Bai, Adelino V. M. Canario, Xiaoyan Xu, Jiale Li
Summary: This study found that lncRNA is involved in regulating immune function in black carp. Specific signaling pathways and genes were identified to be associated with black carp resistance to Aeromonas hydrophila, while other signaling pathways were related to susceptibility.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jyot D. Antani, Rachit Gupta, Annie H. Lee, Kathy Y. Rhee, Michael D. Manson, Pushkar P. Lele
Summary: The bacterial flagellar motor is able to adapt its switching between clockwise and counterclockwise rotation in response to changes in mechanical load by controlling the binding affinity of the chemotaxis response regulator, CheY-P. This allows the motor to maintain optimal function in environments of varying viscosities. The interplay between mechanical forces and CheY-P binding tunes the chemotactic function to match the load, resembling proprioceptive feedback in neuromuscular systems.
NATURE COMMUNICATIONS
(2021)
Article
Fisheries
Chanitcha Choolert, Phongthana Pasookhush, Akapon Vaniksampanna, Siwaporn Longyant, Parin Chaivisuthangkura
Summary: In this study, a homolog of TRAF6 called MrTRAF6 was identified and characterized. It was found that MrTRAF6 is involved in various biological processes, including adaptive and innate immunity. Gene expression analysis showed that MrTRAF6 is expressed in all tissues and plays a critical role in antibacterial activity.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Cell Biology
Norihiro Takekawa, Tatsuro Nishikino, Kiyoshiro Hori, Seiji Kojima, Katsumi Imada, Michio Homma
Summary: Bacteria exhibit chemotaxis by controlling flagellar rotation, with the ZomB protein playing a crucial role in influencing the direction of bacterial flagellar rotation. It interacts with CheY-P and may also be involved in reactions with other proteins. This study confirms the important role of ZomB in Vibrio alginolyticus.
Article
Microbiology
Tsai-Shun Lin, Seiji Kojima, Hajime Fukuoka, Akihiko Ishijima, Michio Homma, Chien-Jung Lo
Summary: The bacterial flagellar motor (BFM) is a large membrane-spanning molecular rotary machine that adjusts the number of stator units to compensate for changes in external load and ion-motive force to maintain constant speed over a wide range of sodium concentrations. When rapidly transitioning from high to low sodium concentrations, the number of functional stator units experiences a rapid excessive reduction followed by resurrection, implying the presence of a metastable hidden state during the sudden loss of sodium ions.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Trishant R. Umrekar, Yvonne B. Winterborn, Shamphavi Sivabalasarma, Julian Brantl, Sonja-Verena Albers, Morgan Beeby
Summary: The novelty in biology often arises from opportunistic repurposing of existing features, such as the emergence of rotary motors like archealla. Understanding the evolutionary process at the molecular scale presents challenges due to a lack of case studies. Through the study of ArlG filaments, it was discovered that their open helical structure differs from the closed helical filaments of ArlB, shedding light on the evolutionary neofunctionalization of the archaellar stator complex.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Fisheries
Yujie Yuan, Zechao Shi, Qin Wang, Mengge Guo, Le Yuan, Zhangchun Zhao, Sixue Liu, Chen Wu, Ruhan Sun, Bingchao Wang, Gang Ouyang, Wei Ji
Summary: The MyD88-dependent pathway mediated by Toll-like receptor is crucial in activating immune responses in yellow catfish. The cloned and characterized genes Pf_MyD88, Pf_IRAK4, Pf_IRAK1, Pf_TRAF6 and Pf_NF kappa B1 showed high conservation across species and exhibited the highest similarity to genes in other fish species. These genes were highly expressed in the liver and showed similar expression patterns during different embryonic development stages. After stimulation with inactivated Aeromonas hydrophila, the mRNA expressions of these genes were significantly increased in various tissues, indicating their involvement in the innate immune response of yellow catfish. These findings highlight the importance of the MyD88-dependent signaling pathway in disease defense and provide insights for the application of inactivated vaccines against bacterial diseases in teleost.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Hala F. Ayoub, Mohamed F. Abdelghany, Saad M. Alsaiad, Amel M. El Asely
Summary: In this study, the application of Amphora coffeaeformis diatom in the diet of Nile tilapia was found to improve growth performance, enhance immune and antioxidant responses, and control Aeromonas hydrophila infection. The 2% and 3% concentrations of A. coffeaeformis showed the most significant effects. Immunological and antioxidant improvements were maintained even after the challenge with A. hydrophila. The histopathological examination also demonstrated the protective effects of A. coffeaeformis against A. hydrophila infection in various organs of Nile tilapia.
AQUACULTURE RESEARCH
(2022)
Article
Engineering, Mechanical
Ai Mizuno, Hidekazu Kajiwara, Hideki Tamura, Manabu Aoyagi
Summary: Most multidegree-of-freedom ultrasonic motors (MDOF-USMs) use a spherical rotor, which restricts the design of the stator and results in a complicated structure. To address this inconvenience, a metal spherical stator design for MDOF-USM was proposed. The rotational vibration mode on the stator was achieved through theoretical analysis and finite element method analysis. However, the obtained torque, power factor, and speed were found to be low, indicating the need for further research to improve the excitation method and rotor structure.
Article
Chemistry, Physical
Y. C. Chou
Summary: This study proposes a physical mechanism for the torque generation of a bacterial flagellar motor (BFM) by collisions between the stator and rotor, which can generate the impulsive forces required for rotor rotation. It suggests that two mechanisms, asymmetrical rotor fluctuation and collisions between the stator and asymmetric FliG(N) and FliG(M), can generate the torque in the direction of the rotor. These mechanisms may be related to bidirectional rotation, tumbling motion, and the characteristics of the rotational angle.
EUROPEAN PHYSICAL JOURNAL E
(2022)
Article
Fisheries
JaiGopal Sharma, Amarjeet Singh, Ajima Begum, Sonia, Vungarala Hari Krishna, Rina Chakrabarti
Summary: This study evaluated the effects of feeding Achyranthes aspera seeds and leaves on the immune system of magur Clarias batrachus challenged with Aeromonas hydrophila in pond conditions. The results showed that the inclusion of A. aspera in the diet improved the immune system of the fish, reduced mortality rates, and promoted growth. Additionally, the feeds also lowered oxidative stress levels and increased antioxidant levels in the fish.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gabriel Forn-Cuni, Susana Merino, Juan M. Tomas
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2017)
Article
Fisheries
Jie Ji, Susana Merino, Juan M. Tomas, Nerea Roher
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Genetics & Heredity
Mario Huttener, Alejandro Prieto, Sonia Aznar, Manuel Bernabeu, Estibaliz Glaria, Annabel F. Valledor, Sonia Paytubi, Susana Merino, Joan Tomas, Antonio Juarez
Correction
Microbiology
Markus Wilhelms, Raquel Molero, Jonathan G. Shaw, Juan M. Tomas, Susana Merino
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Gabriel Forn-Cuni, Kelly M. Fulton, Jeffrey C. Smith, Susan M. Twine, Elena Mendoza-Barbera, Juan M. Tomas, Susana Merino
Summary: This study demonstrates that genes involved in polar flagella glycosylation in mesophilic Aeromonas strains are clustered in highly polymorphic genomic islands. Bioinformatic analysis identified three types of polar flagella glycosylation islands, corresponding to modifications with single monosaccharide and heterogeneous glycans. Group II strains contain a common glycosyltransferase, fgi-1, downstream of luxC, which transfers the first sugar of heterogeneous glycans to the pseudaminic acid derivative linked to flagellins.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rossella Di Guida, Angela Casillo, Juan M. Tomas, Susana Merino, Maria Michela Corsaro
Summary: Aeromonas species are pathogenic bacteria found in various environments and foods. In this study, the structure of the O-chain repeating unit of the LPS from Aeromonas bivalvium strain 868 E-T was determined, providing insights into the pathogenicity of this species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Medicinal
Elena Mendoza-Barbera, Susana Merino, Juan Tomas
Summary: Aeromonas spp. are commonly found in aquatic environments and can also be isolated from fresh and processed food. They mainly infect poikilothermic animals, but are also considered opportunistic pathogens of aquatic and terrestrial homeotherms, with some species associated with gastrointestinal and extraintestinal septicemic infections in humans.
Article
Microbiology
Mario Huttener, Jon Hergueta, Manuel Bernabeu, Alejandro Prieto, Sonia Aznar, Susana Merino, Joan Tomas, Antonio Juarez
Summary: Horizontal transfer of bacterial plasmids plays a crucial role in the dissemination of antimicrobial resistance (AMR) genes. This study identifies novel high molecular-weight proteins encoded by plasmid genes, which facilitate plasmid transmission by regulating cell motility and cell-to-cell contact. The findings may provide a potential strategy for controlling the spread of AMR plasmids.
Article
Multidisciplinary Sciences
Juan M. Tomas, Kelly M. Fulton, Susan M. Twine, Susana Merino
Summary: The study of glycosylation in prokaryotes is a rapidly growing field, with bacteria harboring strain-specific glycans on their surface that play important roles in host recognition processes and interaction with the environment.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Maria Jordan, Sylwia Wojtys-Tekiel, Susana Merino, Juan M. Tomas, Marta Kaszowska
Summary: The Edwardsiella genus consists of five species that can cause infections in fish, reptiles, birds, and humans. The lipopolysaccharide (LPS) core oligosaccharides of E. piscicida, E. anguillarum, E. hoshinae, and E. ictaluri were studied for the first time, including their chemical structure and genomics. The structures of the core oligosaccharides were determined using H-1 and C-13 nuclear magnetic resonance spectroscopy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Tien-Tien Vicky Lau, Suat-Moi Puah, Jin-Ai Mary Anne Tan, Susana Merino, S. D. Puthucheary, Kek-Heng Chua
Summary: This study investigates the role of flagellar genes in the biofilm formation of Aeromonas dhakensis. Results show that mutants lacking certain flagellar genes exhibited reduced swimming, swarming, and biofilm formation. However, complemented strains with these genes restored wild-type levels of motility and biofilm formation. This suggests that these flagellar genes play an important role in biofilm formation through flagella-mediated motility and surface attachment.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Mario Huttener, Jon Hergueta, Manuel Bernabeu, Alejandro Prieto, Sonia Aznar, Susana Merino, Joan Tomas, Antonio Juarez
Summary: The horizontal transfer of bacterial plasmids generates genetic variability and contributes to the dissemination of genes that enable bacterial cells to develop anti-microbial resistance. Newly identified proteins that influence the conjugation of multiple plasmids have been described in this work. These proteins, encoded by plasmid genes, interact with extracellular organelles and facilitate conjugation by reducing cell motility and promoting cell-to-cell contact. They may play important roles in the dissemination of plasmids in natural environments and could be targeted to control the spread of AMR-carrying bacterial plasmids.
