Article
Microbiology
Tobias Busche, Hana Dostalova, Lenka Rucka, Jiri Holatko, Ivan Barvik, Vaclav Stepanek, Miroslav Patek, Joern Kalinowski
Summary: The sigma H and sigma E subunits of Corynebacterium glutamicum RNA polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) sigma factors. While the genes of the C. glutamicum sigma(Eta) regulon involved in heat and oxidative stress response have been studied, the genes of the sigma(E) regulon, involved in cell surface stress response, have not been explored until now. Differential gene expression analysis revealed a significant overlap between the sigma(Eta) and sigma(E) regulons, and identified genes involved in protein quality control, regulation of Clp proteases, and membrane integrity maintenance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Giovanni Versace, Marta Palombo, Anna Menon, Vincenzo Scarlato, Davide Roncarati
Summary: The heat-shock response is a universal protective mechanism that involves transcriptional reprogramming of cellular transcriptome to counteract the deleterious effects of heat-stress. In Campylobacter jejuni, the response is controlled by two repressors, HspR and HrcA, with HrcA acting as an intrinsic protein thermometer. The DNA-binding activity of HrcA is negatively affected by temperature upshift, while HspR remains stable under heat-stress conditions.
Article
Microbiology
Yuya Tanaka, Hamamoto Nagisa, Sawa Masato, Masayuki Inui
Summary: In this study, we found that RNase J regulates its own gene by degrading its own mRNA and induces the expression of rnj mRNA under cold-shock conditions. This is important for understanding the regulatory mechanism of RNase J in Corynebacterium glutamicum.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Hao Zhang, Zhilin Ouyang, Nannan Zhao, Shuangyan Han, Suiping Zheng
Summary: In this study, the AmtR regulon in C. glutamicum ATCC 14067 was identified using ChIP-seq, revealing new operons related to creatine and creatinine degradation. The negative regulation of transcription and expression of these genes by AmtR was confirmed. Additionally, it was found that C. glutamicum ATCC 14067 can utilize creatine or creatinine as a sole nitrogen source.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ana Sanchez-Jimenez, Maria A. Llamas, Francisco Javier Marcos-Torres
Summary: Pseudomonas aeruginosa is a versatile pathogen capable of colonizing human tissues. Its colonization and virulence are driven by various regulated virulence factors. This study reviews the transcriptional regulators that control the expression of these factors and proposes blocking these regulators as a promising strategy to combat this clinically relevant pathogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jianxiao Zhao, Xi Sun, Zhitao Mao, Yangyang Zheng, Zhouxiao Geng, Yuhan Zhang, Hongwu Ma, Zhiwen Wang
Summary: Gene expression in bacteria is regulated by multiple transcription factors. In this study, we used independent component analysis to analyze the gene expression profile of Corynebacterium glutamicum and constructed a quantitative transcriptional regulatory network. We identified potential targets for transcription factors and observed changes in the network under different growth and environmental conditions.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Nannan Zhao, Jian Wang, Aiqing Jia, Ying Lin, Suiping Zheng
Summary: A putrescine-specific biosensor (pSenPuuR) was developed in Corynebacterium glutamicum using Escherichia coli-derived transcriptional factor PuuR. The response characteristics of the biosensor to putrescine were improved by optimizing the genetic components of pSenPuuR. The study suggests that pSenPuuR has the potential to assess putrescine production in C. glutamicum and is suitable for high-throughput genetic variant screening.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Qiaoyun Zhu, Qi Liu, Chaoying Yao, Yuanxing Zhang, Menghao Cai
Summary: In this study, yeast transcriptional device libraries were developed for precise synthesis of value-added chemicals from methanol. The transcriptional devices showed good activity and expression strength in a methylotrophic yeast. By selecting and optimizing the devices, the production of chemicals from methanol can be significantly increased.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Charlotte R. Cooper, April M. Lewis, Jaspreet S. Notey, Arpan Mukherjee, Daniel J. Willard, Paul H. Blum, Robert M. Kelly
Summary: Thermoacidophilic archaea lack sigma factors and HSPs, but have other strategies like transcriptional regulators and type II TA loci to handle thermal stress. The study on Saccharolobus solfataricus 98/2 showed that genes like YtrA(Ss), FadR(Ss), VapC22, VapB6, and VapC6 play a role in the interconnected regulatory network during thermal stress response. This suggests a different cellular mechanism for heat shock response in archaea compared to bacteria.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Microbiology
Doglas Parise, Mariana Teixeira Dornelles Parise, Anne Cybelle Pinto Gomide, Flavia Figueira Aburjaile, Rodrigo Bentes Kato, Marisol Salgado-Albarran, Andreas Tauch, Vasco Ariston de Carvalho Azevedo, Jan Baumbach
Summary: Corynebacterium pseudotuberculosis is a Gram-positive, facultative intracellular pathogen that causes serious economic losses in livestock farming. Despite limited understanding of its transcriptional regulatory mechanisms, key players such as the PhoPR system and DtxR are promising targets for future vaccine design and drug development to combat this important zoonotic pathogen.
Article
Biotechnology & Applied Microbiology
Yota Tsuge, Naoto Kato, Shogo Yamamoto, Masako Suda, Masayuki Inui
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Biochemical Research Methods
Akiyoshi Higo, Shigeki Ehira
ACS SYNTHETIC BIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Yota Tsuge, Naoto Kato, Shogo Yamamoto, Masako Suda, Toru Jojima, Masayuki Inui
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Microbiology
Akiyoshi Higo, Eri Nishiyama, Kota Nakamura, Yukako Hihara, Shigeki Ehira
JOURNAL OF BACTERIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Satoshi Hasegawa, Toru Jojima, Masako Suda, Masayuki Inui
METABOLIC ENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Hiroto Uchikura, Koichi Toyoda, Hiroki Matsuzawa, Hikaru Mizuno, Kazuaki Ninomiya, Kenji Takahashi, Masayuki Inui, Yota Tsuge
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Takahisa Kogure, Masako Suda, Kazumi Hiraga, Masayuki Inui
Summary: Protocatechuic acid (PCA) is a natural bioactive phenolic acid that can be produced by Corynebacterium glutamicum through the DHS and 4-HBA pathways. Simultaneous utilization of both pathways is an efficient method for PCA production, with the highest reported productivity reaching 82.7 g/L.
METABOLIC ENGINEERING
(2021)
Article
Biochemical Research Methods
Shiori Nakazawa, Osamu Imaichi, Takahisa Kogure, Takeshi Kubota, Koichi Toyoda, Masako Suda, Masayuki Inui, Kiyoto Ito, Tomokazu Shirai, Michihiro Araki
Summary: The technique involves computational suggestion of genetic modifications based on design history, retrieval of well-known designs related to the history, and enhancement of production through experimental modification. The successful utilization of former cycles suggests relevant designs worth testing, ultimately aiding in the development of bioprocessing.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Physical
Haruhiko Teramoto, Tetsu Shimizu, Masako Suda, Masayuki Inui
Summary: This study explored the genetic engineering of Escherichia coli for hydrogen production. The introduction of exogenous hydrogenase and inactivation of endogenous hydrogenases resulted in both hydrogen production and uptake. Disabling the pathways for ethanol, lactate, and succinate production significantly increased hydrogen production. Unexpectedly, high expression of exogenous hydrogenase completely repressed hydrogen production, and the introduction of heterologous enzyme repressed the endogenous hydrogen production ability.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Microbiology
Koichi Toyoda, Riki Sugaya, Akihiro Domon, Masako Suda, Kazumi Hiraga, Masayuki Inui
Summary: Understanding the metabolic pathways and regulation of amino acids is crucial for optimizing amino acid production. This study identified and characterized the ans operon that is involved in the catabolism of L-Asn in Corynebacterium glutamicum R. The operon consists of genes encoding a transcriptional regulator (AnsR), asparaginase (AnsA2), aspartase (AspA2), and permease (AnsP). The enzymes and permease encoded by this operon are essential for the utilization of L-Asn. The study also revealed the regulatory mechanism of the operon, with AnsR acting as a transcriptional repressor that is inhibited by L-Asn.
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
Summary: This study provides a novel approach for increasing the yield of photofermentative H-2 production from acetate by purple nonsulfur phototrophic bacteria. This study further suggests that polyhydroxyalkanoate is not only a storage substance for carbon and energy in bacteria, but may also act as a signaling molecule that mediates bacterial metabolic adaptations to specific environments.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Haruhiko Teramoto, Masako Suda, Masayuki Inui
Summary: This study investigates the inhibitory compounds in dilute sulfuric acid-pretreated corn stover on hydrogen production by Escherichia coli. The results indicate that acetate is the primary inhibitory factor.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Biotechnology & Applied Microbiology
Akiyoshi Higo, Shigeki Ehira
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)