Journal
JOURNAL OF BACTERIOLOGY
Volume 190, Issue 24, Pages 8238-8243Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.00889-08
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [(B)18780060]
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The transcriptional regulator TyrR is known to undergo a dimer-to-hexamer conformational change in response to aromatic amino acids, through which it controls gene expression. In this study, we identified N316D as the second-site suppressor of Escherichia coli TyrR(E274Q), a mutant protein deficient in hexamer formation. N316 variants exhibited altered in vivo regulatory properties, and the most drastic changes were observed for TyrR(N316D) and TyrR(N316R) mutants. Gel filtration analyses revealed that the ligand-mediated oligomer formation was enhanced and diminished for TyrR(N316D) and TyrR(N316R), respectively, compared with the wild-type TyrR. ADP was substituted for ATP in the oligomer formation of TyrR(N316D).
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