Article
Endocrinology & Metabolism
Yan Deng, Hu Li, Yi Song, Juan Cen, Yuying Zhang, Yi Sui, Dexuan Cui, Tin Chiu Li, Yan Xu, Chi Chiu Wang, Pui Wah Jacqueline Chung, Tao Tang
Summary: This study investigated the lncRNA and mRNA profiles in ovarian granulosa cells from women with PCOS and identified DLGAP5 as a dysregulated candidate gene involved in the pathogenesis of PCOS, particularly in granulosa cell apoptosis and proliferation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Reproductive Biology
Patrick R. Hannon, James W. Akin, Thomas E. Curry Jr
Summary: Exposure to phthalates disrupts ovarian function and inhibits PGR signaling in human granulosa cells, but supplementation with cAMP can restore PGR signaling and potentially alleviate the ovulatory dysfunction caused by phthalate exposure.
BIOLOGY OF REPRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Datu Agasi Mohd Kamal, Siti Fatimah Ibrahim, Azizah Ugusman, Mohd Helmy Mokhtar
Summary: This study found aberrant expression of sex steroid receptors in polycystic ovary syndrome and demonstrated that Kelulut honey treatment can normalize the expression of these receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Alice Bongrani, Namya Mellouk, Christelle Rame, Marion Cornuau, Fabrice Guerif, Pascal Froment, Joelle Dupont
Summary: Vaspin is highly expressed in the human ovary and enhances granulosa cells steroidogenesis, proliferation, and viability through GRP78 in a concentration-dependent manner. Obese women and the normal-weight ECHO group show significantly higher levels of vaspin in both granulosa cells and follicular fluid, with the ECHO group also exhibiting the highest expression rates of GRP78.
JOURNAL OF ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jing Luo, Hong Ye, Lijuan Hao, Yixuan Sun, Ruohan Li, Yanxi Li, Zhu Yang
Summary: The study identified the presence of AR alternative splicing variants AR-SVs in PCOS patients' GCs, leading to reduced AR function. miRNA-183 and miRNA-124 were found to regulate the expression of SRSF2 and SRSF3, influencing AR function and androgen accumulation in the ovary.
Article
Obstetrics & Gynecology
Joie Z. Guner, Diana Monsivais, Henry Yu, Fabio Stossi, Hannah L. Johnson, William E. Gibbons, Martin M. Matzuk, Stephen Palmer
Summary: TOP5300 appears to stimulate higher E2 production and steroidogenic gene expression in human granulosa cells from patients with polycystic ovary syndrome (PCOS) compared to recombinant human FSH (rh-FSH). FSH receptor (FSHR) membrane localization does not seem to be a limiting step for the stimulation of steroidogenic gene expression and E2 production by TOP5300 or rh-FSH.
FERTILITY AND STERILITY
(2023)
Review
Biochemistry & Molecular Biology
Ahmad J. H. Albaghdadi, Frederick W. K. Kan
Summary: PCOS is a major anovulatory infertility affecting a great proportion of women of childbearing age, associated with obesity, insulin resistance, and chronic inflammation. Conventional therapeutic interventions have limited success in restoring ovulation and endometrial receptivity in women with PCOS, while low-dose tacrolimus shows potential therapeutic benefits in treating female infertility associated with PCOS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Myung Jae Jeon, Young Sik Choi, James J. Yoo, Anthony Atala, John D. Jackson
Summary: The study aimed to develop an optimized culture system for maintaining ovarian cell growth and functionality. By isolating granulosa cells and theca cells from female rats and adding follicle-stimulating hormone or luteinizing hormone to the basal culture media, secretion of estradiol and androstenedione hormones could be significantly enhanced. Additionally, growth differentiation factor 9, bone morphogenic protein 15, BMP7 and basic fibroblast growth factor were found to enhance granulosa cell proliferation and estradiol production.
CELL AND TISSUE RESEARCH
(2021)
Article
Endocrinology & Metabolism
Borhan Shokrollahi, Hai-Ying Zheng, Ling-Yu Li, Li-Ping Tang, Xiao-Ya Ma, Xing-Rong Lu, An-Qin Duan, Yu Zhang, Xiao-Hui Tan, Chen-Xi Huang, Yuan-Yuan Xu, Jiang-Hua Shang
Summary: This study investigated the expression levels of APLN/APJ in buffalo ovarian granulose cells (GCs) and the effects of IGF1 and FSH on APLN/APJ expression. The results showed that APLN expression was highest in large-sized GCs, while APJ expression increased with follicle enlargement. IGF1 and FSH increased the expression of APLN and FSH, and IGF1 also increased the expression of APJ. Both isoforms of APLN increased the secretion of E2 and P4, potentially through upregulation of CYP19A1 and StAR expression. In conclusion, APLN may play a crucial role in steroidogenesis and follicular development in buffalo ovarian GCs.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Andrology
Christina Messini, Anna Vasilaki, Evangelia Korona, George Anifandis, Panagiotis Georgoulias, Konstantinos Dafopoulos, Antonios Garas, Alexandros Daponte, Ioannis E. Messinis
Summary: This study revealed that adiponectin can stimulate the secretion of estradiol and progesterone by luteinized granulosa cells in a dose-dependent manner, potentially playing a role in sensitizing granulosa cells to FSH in human ovarian steroidogenesis.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Salvatore Giovanni Vitale, Anna Maria Fulghesu, Mislav Mikus, Rafal Watrowski, Maurizio Nicola D'Alterio, Li-Te Lin, Mohsin Shah, Enrique Reyes-Munoz, Thozhukat Sathyapalan, Stefano Angioni
Summary: MicroRNAs (miRNAs) play crucial regulatory roles in tissue development, differentiation, and metabolic processes. In the context of PCOS, altered miRNA levels have been associated with hormonal changes, glucose and lipid metabolism, and fertility issues. This systematic review provides an overview of miRNA expression, hormonal changes, and metabolic correlations in PCOS, highlighting the potential of miRNAs as therapeutic targets and diagnostic markers.
Article
Biochemistry & Molecular Biology
Sijie Fan, Wenjing Lu, Haolin Zhang, Zhengrong Yuan, Yingying Han, Qiang Weng
Summary: This study aims to investigate the relationship between the concentration of adiponectin, ovarian tissue morphology, ovarian steroidogenesis, and sex hormone production in ovaries of wild ground squirrels. The results show that the ovarian mass, circulating estradiol and progesterone concentrations were significantly higher during the breeding season, while the circulating adiponectin level was significantly lower. The expression levels of gonadotropin receptors and steridogenic enzymes were significantly higher during the breeding season. The study suggests that adiponectin may be involved in the regulation of seasonal changes in the ovarian function of wild ground squirrels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yanling Wu, Belen Chanclon, Peter Micallef, Elisabet Stener-Victorin, Ingrid Wernstedt Asterholm, Anna Benrick
Summary: The study demonstrates that elevated levels of adiponectin in utero can improve insulin sensitivity, reduce body weight and fat mass gain in adult offspring, and protect against PNA-induced visceral adiposity in the offspring.
Article
Biology
Min Chen, Fangfang Dong, Min Chen, Zhiming Shen, Haowei Wu, Changhuo Cen, Xiuhong Cui, Shilai Bao, Fei Gao
Summary: PRMT5 plays a crucial role in follicle development by regulating WT1 expression and facilitating the translation of Wt1 mRNA through methylation of HnRNPA1. It is essential for granulosa cell lineage maintenance, follicle structure, and steroidogenesis-related gene expression.
Review
Obstetrics & Gynecology
Xuan Duan, Meiying Zhou, Guangqin Zhou, Qiyu Zhu, Weihong Li
Summary: The meta-analysis showed that metformin treatment significantly increased serum adiponectin concentrations in women with PCOS, while also improving indicators such as TNF-α and CRP levels. Further research with a larger sample size is needed to confirm the optimal dose and duration of metformin treatment.
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY
(2021)