Article
Endocrinology & Metabolism
Jing Ling Ruan, Shan Shan Liang, Jia Ping Pan, Zhi Qin Chen, Xiao Ming Teng
Summary: This retrospective study found that artificial oocyte activation (AOA) with calcium ionophore ionomycin can significantly improve the reproductive outcomes of patients with previous fertilization failure and poor embryo development after intracytoplasmic sperm injection (ICSI). AOA can increase fertilization rate, embryo quality, implantation rate, clinical pregnancy rate, and live birth rate, while decreasing miscarriage rate. The study suggests that AOA is an effective and safe treatment option for some infertility patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Lei Jia, Panyu Chen, Wenlong Su, Shujing He, Yingchun Guo, Lei Zheng, Cong Fang, Xiaoyan Liang
Summary: This retrospective cohort study compared the fertilization rates in ICSI cycles treated with artificial oocyte activation (AOA) using 10 mu mol/l ionomycin or commercial A23187. The results showed that the fertilization rates were significantly higher in the ionomycin group compared to the A23187 group for all subgroups. Therefore, AOA with 10 mu mol/l ionomycin may be more effective in improving oocyte activation, especially in cases of sperm-related defects.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Review
Obstetrics & Gynecology
Bei Sun, John Yeh
Summary: This paper reviews the effects of calcium oscillatory patterns in oocytes and early embryo development, suggesting that abnormal calcium oscillations may be the primary mechanism for total fertilization failure (TFF). Studies also found strong associations between abnormal calcium oscillatory patterns and suboptimal developmental outcomes. Empirical use of artificial oocyte activation (AOA) methods has shown initial success in helping TFF patients.
REPRODUCTIVE SCIENCES
(2021)
Article
Health Care Sciences & Services
Ioana Zaha, Petronela Naghi, Liana Stefan, Cosmina Bunescu, Mihaela Radu, Mariana Eugenia Muresan, Mircea Sandor, Liliana Sachelarie, Anca Huniadi
Summary: The microfluidic sperm selection method was found to improve the outcomes of in vitro fertilization (IVF) procedures. This method, which selects spermatozoa with the lowest DNA fragmentation rate, resulted in higher blastocyst rates and clinical pregnancy rates. It has the potential to be widely used in intracytoplasmic sperm injection (ICSI) and improve the workflow of standard IVF, reducing intervention by laboratory personnel and providing more consistent incubation conditions.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Cell Biology
Meng Wang, Lixia Zhu, Chang Liu, Hui He, Cheng Wang, Chenxi Xing, Jinming Liu, Liu Yang, Qingsong Xi, Zhou Li, Lei Jin
Summary: The study demonstrates that a new AOA method combining cycloheximide and ionomycin significantly increases fertilization rates for most TFF patients, potentially offering therapeutic benefits for couples experiencing severe fertilization deficiencies even after conventional AOA treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Tzung-En Tsai, Pei-Hsuan Lin, Pei-Fen Lian, Chia-Jung Li, Salvatore Giovanni Vitale, Mislav Mikus, Wan-Ping Su, Hsiao-Wen Tsai, Kuan-Hao Tsui, Li-Te Lin
Summary: AOA may improve embryo quality in older patients with diminished ovarian reserve undergoing IVF-ICSI cycles, especially in women aged >= 40 years old.
JOURNAL OF OVARIAN RESEARCH
(2022)
Article
Cell Biology
Haixia Chen, Peng Li, Xiaoling Du, Yiding Zhao, Lingling Wang, Ye Tian, Xueru Song, Ling Shuai, Xiaohong Bai, Lingyi Chen
Summary: Mutation in the SEPTIN12 gene is associated with male infertility. Male mice with Septin12 knockout are infertile and have abnormal sperm, but fertility can be restored with artificial oocyte activation. Loss of PLC zeta might be the reason for failed fertilization in Septin12-deficient sperm.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Genetics & Heredity
Xiangjun Zhang, Congyuan Hu, Limin Wu
Summary: Fertilization failure refers to the failure in pronucleus formation after 16-18 hours of in vitro fertilization or intracytoplasmic sperm injection, which can be caused by various factors such as sperm, oocytes, and sperm-oocyte interaction. Recent advancements in genetics, molecular biology, and clinical-assisted reproductive technology have greatly contributed to the understanding and treatment of fertilization failure. This review provides an overview of the causes and treatment methods for fertilization failure, highlighting the latest research advances in the genetic aspects of this condition.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Endocrinology & Metabolism
Camille Robin, Audrey Uk, Christine Decanter, Helene Behal, Pierre Collinet, Chrystele Rubod, Anne-Laure Barbotin, Geoffroy Robin
Summary: The study aimed to evaluate the relationship between endometriosis and oocyte morphology after controlled ovarian stimulation for intracytoplasmic sperm injection (ICSI) on a large oocyte cohort, finding that endometriosis does not have a negative impact on oocytes' morphology in IVF-ICSI.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Obstetrics & Gynecology
Jing Wang, Jintao Zhang, Xueping Sun, Yuting Lin, Lingbo Cai, Yugui Cui, Jiayin Liu, Mingxi Liu, Xiaoyu Yang
Summary: The study identified novel compound heterozygous variants in ACTL7A as a genetic factor for human TFF and successfully rescued TFF through AOA.
HUMAN REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Olga Tepla, Zinovij Topurko, Simona Jirsova, Martina Moosova, Eva Fajmonova, Radek Cabela, Katerina Komrskova, Irena Kratochvilova, Jaromir Masata
Summary: The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. The visualization of MS influenced the pregnancy rate (PR) for patients over 35 years, but did not affect PR for patients under 35 years. Visualization of MS appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Ziwen Xu, Guidong Yao, Wenbin Niu, Huiying Fan, Xueshan Ma, Senlin Shi, Haixia Jin, Wenyan Song, Yingpu Sun
Summary: The study found that using calcium ionophore (A23187) for rescue activation of unfertilized oocytes can obtain a certain proportion of high-quality blastocysts with normal karyotype, and it does not significantly affect the timing of embryo development. In clinical practice, direct activation with calcium ionophore (A23187) after ICSI is more effective than rescue activation the next day.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Genetics & Heredity
Sophie Popkiss, Fabrizzio Horta, Beverley Vollenhoven, Mark P. Green, Deirdre Zander-Fox
Summary: This study aimed to investigate the effect of calcium chloride dihydrate supplementation on different fertilization rate groups during the ICSI process. The results showed that adding calcium significantly improved fertilization rate and clinical pregnancy rate in patients with low fertilization rates (< 50%), but had minimal impact on patients with normal fertilization rates.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Obstetrics & Gynecology
Takashi Morimoto, Tomohiro Maekawa, Shimpei Mizuta, Hidehiko Matsubayashi, Takumi Takeuchi, Yutaka Hata, Tomomoto Ishikawa
Summary: During the intracytoplasmic sperm injection (ICSI) procedure, unintentional membrane rupture (UMR) often leads to oocyte degeneration. The study showed that the ICSI Position Detector (IPD) can be helpful in identifying the best puncture location to prevent UMR and improve the success of the procedure.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Cell Biology
Gustavo Luis Veron, Maria Florencia Veiga, Monica Cameo, Clara Isabel Marin-Briggiler, Monica Hebe Vazquez-Levin
Summary: This study aimed to characterize the expression of E-cad and N-cad in murine gametes and their involvement in fertilization. The results showed that these proteins play important roles in various steps of mammalian fertilization, including adhesion, penetration, and fusion.