Article
Biochemistry & Molecular Biology
Cristina Cuello, Cristina A. Martinez, Josep M. Cambra, Inmaculada Parrilla, Heriberto Rodriguez-Martinez, Maria A. Gil, Emilio A. Martinez
Summary: This study investigated the impact of vitrification on the transcriptome profile of porcine blastocysts. The results showed that vitrification altered the gene expression of blastocysts, with minor changes mainly related to cell cycle, cellular senescence, gap junction, and signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Tayita Suttirojpattana, Theesit Juanpanich, Rangsun Parnpai, Teraporn Vutyavanich
Summary: The study established a simplified, reliable closed HFV vitrification system using a hemi-straw, which does not require skilled practitioners. In the experiment, a 100% survival rate was achieved in two-cell embryos, while blastocyst development was affected by different vitrification protocols.
ANIMAL SCIENCE JOURNAL
(2021)
Article
Veterinary Sciences
Alejandro Gonzalez-Plaza, Josep M. Cambra, Inmaculada Parrilla, Maria A. Gil, Emilio A. Martinez, Cristina A. Martinez, Cristina Cuello
Summary: The study found that the open Cryotop (R) system is suitable for simultaneous vitrification of 20 porcine embryos at different developmental stages. The viability and quality results of embryos vitrified with this system were comparable to those of fresh embryos subjected to 24 hours of in vitro culture.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
Alejandro Gonzalez-Plaza, Josep M. Cambra, Manuela Garcia-Canovas, Inmaculada Parrilla, Maria A. Gil, Emilio A. Martinez, Heriberto Rodriguez-Martinez, Cristina A. Martinez, Cristina Cuello
Summary: This study investigated the changes in the transcriptome of pig blastocysts caused by vitrification using the OC and SOPS systems. The results showed that vitrification with the OC system resulted in fewer genes related to apoptosis and activated genes related to cell proliferation compared to the SOPS system. However, further investigation is needed to understand how these transcriptome differences affect the subsequent developmental ability of the embryos after transfer.
Article
Agriculture, Dairy & Animal Science
Tengteng Xu, Chengxue Liu, Mengya Zhang, Xin Wang, Yelian Yan, Qiuchen Liu, Yangyang Ma, Tong Yu, Anucha Sathanawongs, Jun Jiao, Zubing Cao, Yunhai Zhang
Summary: This study found that vitrification reduces the developmental efficiency of pig blastocysts by decreasing transcriptional activity during embryonic genome activation and disrupting gene expression. These findings suggest that vitrification impairs genome activation in pig embryos.
Article
Obstetrics & Gynecology
Katerina Chatzimeletiou, Antonia Sioga, Nikos Petrogiannis, Yannis Panagiotidis, Marialena Prapa, Antonios Patrikiou, Basil C. Tarlatzis, Grigoris Grimbizis
Summary: The study found that vitrified embryos had higher cell damage compared to fresh embryos, regardless of whether they were vitrified in open or closed systems. However, both Day 5 and Day 3 biopsied embryos showed no adverse effects on viability and ultrastructure following vitrification in open and closed systems.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Endocrinology & Metabolism
Ningling Wang, Xinxi Zhao, Meng Ma, Qianqian Zhu
Summary: The study found that there was a significant increase in live birth rate and birth weight after transfer of single good quality embryo on day 5 and day 6 compared with transfer of single good quality embryo on day 3 in vitrified embryo transfer cycles.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Genetics & Heredity
Roni Rahav-Koren, Shmuel Inbar, Netanella Miller, Amir Wiser, Yael Yagur, Chen Berkowitz, Sivan Farladansky-Gershnabel, Adrian Shulman, Arie Berkowitz
Summary: The study suggests that culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts may increase the chances of ongoing pregnancy and delivery compared to transferring thawed blastocysts directly.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Katerina Chatzimeletiou, Nikos Petrogiannis, Antonia Sioga, Elpida-Niki Emmanouil-Nikoloussi, Yannis Panagiotidis, Marialena Prapa, Antonios Patrikiou, Maria Filippa, Glykeria Zervakakou, Kyriakos Papanikolaou, Anastasios Makedos, Efstratios Kolibianakis, Basil C. Tarlatzis, Grigoris Grimbizis
Summary: There were no differences in spindle abnormalities or ultrastructure between embryos biopsied on day 5 and day 3, but higher numbers of abnormalities were observed following vitrification, including abnormal spindles, distended mitochondria, multivesicular bodies, lipofuscin droplets, altered cell junctions, and occasionally excessive accumulation of glycogen granules. The fresh day 3 biopsied group had a lower incidence of damaged cells compared to the fresh day 3+5 group and the vitrified day 5 group.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Reproductive Biology
Julian A. Skidmore, Jane L. Vaughan, Clara M. Malo, Muren Herrid
Summary: Using a novel vitrification kit, higher pregnancy rates were achieved for embryos with a diameter less than 500 μm. The Fibreplug group had higher pregnancy rates compared to the Cryolock group, and the effect of BSA on embryo vitrification depends on the type of embryo storage device and embryo diameter.
Article
Reproductive Biology
Ayumo Ito, Yukiko Katagiri, Satoko Oigawa, Kenji Amano, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Masato Yoneyama, Takahiro Tsuchiya, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Nahomi Umemura, Yuko Hayashi, Yuko Tamaki, Koichi Nagao, Masahiko Nakata
Summary: This study aimed to assess the effects of blastocyst shrinkage on assisted reproductive technology (ART) outcomes and determine a more effective morphological evaluation approach. The study found that blastocyst shrinkage after warming has a negative impact on ART outcomes, while blastocyst re-expansion is associated with successful pregnancy and live birth rates. The results of this study can provide valuable information for medical practitioners and patients regarding the success rates of frozen-thawed embryo transfer.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Endocrinology & Metabolism
Kenji Ezoe, Nanoha Fujiwara, Tetsuya Miki, Keiichi Kato
Summary: PRL treatment of human blastocysts after vitrification for 120 min can promote trophoblast outgrowth and increase cell adhesion and migration ability. The optimal time for PRL treatment has also been determined. This study provides a theoretical basis for improving the success rate of embryo transplantation in IVF.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Genetics & Heredity
Kemal Ozgur, Murat Berkkanoglu, Hasan Bulut, Levent Donmez, Ayhan Isikli, Kevin Coetzee
Summary: By analyzing the age and morphology scores of blastocysts, top-scoring blastocysts with clinical implantation rates greater than 70% can be selected.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Medicine, General & Internal
Anat Hershko-Klement, Shaul Raviv, Luba Nemerovsky, Tal Rom, Ayelet Itskovich, Danit Bakhshi, Adrian Shulman, Yehudith Ghetler
Summary: This study investigated the predictive value of blastocyst re-expansion as an indicator of treatment success in embryo transfer. The results showed a positive correlation between re-expansion degree and clinical pregnancy rate. This standardized measurement method can be used as a tool for predicting treatment success.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Obstetrics & Gynecology
Manal Kamel Mohamed, Mohamed Amr Hussein El-Noury, Medhat Kamel Amer, Emad Fakhry, Mahmoud Alalfy
Summary: Cryopreservation of human blastocysts using laser artificial shrinkage before vitrification shows better implantation rate and pregnancy outcomes compared to using micro-needle artificial shrinkage. There is a statistically significant difference in live birth rate and higher rate of twins in the laser group, but without statistical significance.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Editorial Material
Obstetrics & Gynecology
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FERTILITY AND STERILITY
(2016)
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Obstetrics & Gynecology
Shu Hashimoto, Tatsuya Nakano, Kazuo Yamagata, Masayasu Inoue, Yoshiharu Morimoto, Yoshiharu Nakaoka
FERTILITY AND STERILITY
(2016)
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Genetics & Heredity
Kazuhisa Tomita, Shinji Sakai, Mehdi Khanmohammadi, Takayuki Yamochi, Shu Hashimoto, Masayuki Anzai, Yoshiharu Morimoto, Masahito Taya, Yoshihiko Hosoi
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2016)
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Genetics & Heredity
Yuki Takahashi, Shu Hashimoto, Takayuki Yamochi, Hiroya Goto, Masaya Yamanaka, Ami Amo, Hiroshi Matsumoto, Masayasu Inoue, Keijiro Ito, Yoshiharu Nakaoka, Nao Suzuki, Yoshiharu Morimoto
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2016)
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Agriculture, Dairy & Animal Science
Masaya Yamanaka, Kazuhisa Tomita, Shu Hashimoto, Hiroshi Matsumoto, Manabu Satoh, Hiromi Kato, Yoshihiko Hosoi, Masayasu Inoue, Yoshiharu Nakaoka, Yoshiharu Morimoto
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2016)
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Genetics & Heredity
Shu Hashimoto, Naoharu Morimoto, Masaya Yamanaka, Hiroshi Matsumoto, Takayuki Yamochi, Hiroya Goto, Masayasu Inoue, Yoshiharu Nakaoka, Hiroaki Shibahara, Yoshiharu Morimoto
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2017)
Article
Agriculture, Dairy & Animal Science
Takayuki Yamochi, Shu Hashimoto, Masaya Yamanaka, Yoshiharu Nakaoka, Yoshiharu Morimoto
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2017)
Article
Endocrinology & Metabolism
Yuko Kitano, Shu Hashimoto, Hiroshi Matsumoto, Takayuki Yamochi, Masaya Yamanaka, Yoshiharu Nakaoka, Aisaku Fukuda, Masayasu Inoue, Tomoaki Ikeda, Yoshiharu Morimoto
GYNECOLOGICAL ENDOCRINOLOGY
(2018)
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Genetics & Heredity
Yodo Sugishita, Naoki Okamoto, Atsushi Uekawa, Takayuki Yamochi, Mariko Nakajima, Chie Namba, Suguru Igarashi, Takumi Sato, Sei Ohta, Makoto Takenoshita, Shu Hashimoto, Akiko Tozawa, Yoshiharu Morimoto, Nao Suzuki
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2018)
Article
Multidisciplinary Sciences
Shuhei Yoshida, Sui Nishiyama, Lisa Lister, Shu Hashimoto, Tappei Mishina, Aurelien Courtois, Hirohisa Kyogoku, Takaya Abe, Aki Shiraishi, Meenakshi Choudhary, Yoshiharu Nakaoka, Mary Herbert, Tomoya S. Kitajima
NATURE COMMUNICATIONS
(2020)
Article
Genetics & Heredity
Naoharu Morimoto, Shu Hashimoto, Masaya Yamanaka, Tatsuya Nakano, Manabu Satoh, Yoshiharu Nakaoka, Hisataka Iwata, Atsushi Fukui, Yoshiharu Morimoto, Hiroaki Shibahara
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2020)
Article
Nutrition & Dietetics
Shinnosuke Komiya, Yuji Naito, Hidetaka Okada, Yoshiyuki Matsuo, Kiichi Hirota, Tomohisa Takagi, Katsura Mizushima, Ryo Inoue, Aya Abe, Yoshiharu Morimoto
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2020)
Article
Obstetrics & Gynecology
Satoshi Mizuno, Yuko Ishikawa, Hiroshi Matsumoto, Manabu Sato, Mamoru Ida, Aisaku Fukuda, Yoshiharu Morimoto
REPRODUCTIVE MEDICINE AND BIOLOGY
(2019)
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Multidisciplinary Sciences
Shu Hashimoto, Masaya Yamanaka, Takayuki Yamochi, Hisataka Iwata, Ryouka Kawahara-Miki, Masayasu Inoue, Yoshiharu Morimoto
SCIENTIFIC REPORTS
(2019)
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Obstetrics & Gynecology
Takuji Nishihara, Kazuya Matsumoto, Yoshihiko Hosoi, Yoshiharu Morimoto
REPRODUCTIVE MEDICINE AND BIOLOGY
(2018)