Article
Andrology
Michal Duracka, Ljubica Belic, Katarina Tokarova, Jana Ziarovska, Miroslava Kacaniova, Norbert Lukac, Eva Tvrda
Summary: This study investigated bacterial profiles of fresh bovine ejaculates and found that bacterial load was correlated with sperm quality, oxidative stress, and inflammatory markers. High bacterial load may lead to decreased sperm quality and trigger oxidative stress and inflammatory response.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2021)
Article
Obstetrics & Gynecology
Michael L. Eisenberg
Summary: This article provides additional perspectives and reviews for the latest edition of the World Health Organization laboratory manual for the examination and processing of human semen, covering historical context, method perspectives, technological enhancements, and future development proposals.
FERTILITY AND STERILITY
(2022)
Article
Agriculture, Dairy & Animal Science
Anna Zmudzinska, Mariusz A. Bromke, Rafal Strzezek, Magdalena Zielinska, Beata Olejnik, Marzena Mogielnicka-Brzozowska
Summary: This study performed mass spectrometry and bioinformatic analysis on intracellular and membrane-associated proteins in the epididymal sperm of dogs for the first time, revealing their involvement in important metabolic pathways and suggesting their potential as markers for sperm quality. Additionally, sonication was shown to be effective in separating the morphological structure and protein of dog epididymal sperm.
Article
Veterinary Sciences
Mariana Andrade Torres, Victor Henrique Bittar Rigo, Diego Feitosa Leal, Ana Paula Pinoti Pavaneli, Bruno Bracco Donatelli Muro, Joao Diego de Agostini Losano, Giulia Kiyomi Vechiato Kawai, Maite Del Collado, Felipe Perecin, Marcilio Nichi, Simone Maria Massami Kitamura Martins, Andre Furugen Cesar de Andrade
Summary: The study found that trans-resveratrol at low concentrations did not have harmful effects on boar semen storage in vitro but had catastrophic effects on embryo viability. In addition, trans-resveratrol at 1 mM showed harmful effects for most parameters.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Fabio Herrera, Sergey Boryshpolets, Jan Mraz, Jindriska Knowles, Olga Bondarenko
Summary: This study investigated the effects of environmental osmolality and ion composition on pikeperch spermatozoa motility. The research identified potential markers for sperm quality in fish and found that the activation of pikeperch spermatozoa is mainly controlled by ion composition.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Veterinary Sciences
Giacomo Galdiero, Emanuele D'Anza, Cristina de Angelis, Sara Albarella, Vincenzo Peretti, Rosario Pivonello, Francesca Ciotola
Summary: Male infertility is a growing issue in both humans and animals, and understanding its causes is crucial for identifying new diagnostic and therapeutic strategies. This study discovered a correlation between sperm global DNA methylation (SGDM) levels and conventional seminal parameters in dogs, suggesting SGDM could serve as a marker of testis function and spermatogenesis.
VETERINARY SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sameh A. Abdelnour, Mahmoud A. E. Hassan, Ahmed. M. Shehabeldin, Mohamed. E. A. Omar, Wael A. Khalil, Reham Mokhtar Aman
Summary: The effect of propolis-loaded in nanoliposomes (PRNL) on the quality of cryopreserved buffalo semen was investigated. The addition of 2 or 4μg/mL of PRNL significantly improved sperm motility, viability, and membrane integrity, while reducing abnormalities and chromatin damage.
SCIENTIFIC REPORTS
(2023)
Article
Infectious Diseases
Anne-Marie Luther, Thu Quynh Nguyen, Jutta Verspohl, Dagmar Waberski
Summary: The study demonstrated that semen extenders with intrinsic antimicrobial activity can reduce antibiotic use in pig artificial insemination, while maintaining sperm quality.
Article
Reproductive Biology
Aline Fl Paschoal, Anne-Marie Luther, Ulrike Jakop, Martin Schulze, Fernando P. Bortolozzo, Dagmar Waberski
Summary: The study showed that under simulated transport conditions, the response of boar spermatozoa to agitation stress is influenced by the type of semen extender, time after semen processing and temperature. Some samples experienced reduced sperm motility and viability after shaking during storage, while others were unaffected by agitation-induced loss of quality.
Article
Agriculture, Dairy & Animal Science
Monike Quirino, Ulrike Jakop, Ana Paula Goncalves Mellagi, Fernando Pandolfo Bortolozzo, Markus Jung, Martin Schulze
Summary: This study evaluated the effect of different proportions of dead spermatozoa on the quality of liquid boar semen. The results showed that dead sperm did not have a substantial impact on the quality of live sperm.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Genetics & Heredity
Anna A. A. Kapitonova, Vladimir I. I. Muronetz, Denis V. V. Pozdyshev
Summary: Sperm sexing is a technique commonly used in the dairy industry to sort sperm cells based on X or Y chromosomes. This study analyzed the characteristics of sexed semen compared to conventional semen and found that the enzymatic activity of GAPDHS was significantly higher in sexed samples. This enzyme plays a significant role in sperm motility and may contribute to enhancing the fertility characteristics of sexed semen.
Article
Computer Science, Interdisciplinary Applications
Devaraj Somasundaram, Madian Nirmala
Summary: The study introduced a new FRCNN algorithm with ESA for classifying human sperm, along with a new THMA algorithm for motility analysis and tracking, improving the accuracy of CASA.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Review
Agriculture, Dairy & Animal Science
Hiba Ali Hassan, Guillaume Domain, Gaia Cecilia Luvoni, Rana Chaaya, Ann Van Soom, Eline Wydooghe
Summary: Epididymal semen from dogs and cats provides an additional source of gametes for preserving valuable genetics, especially in cases where ejaculation is not possible, castration is necessary, or unexpected death occurs. Understanding the differences between epididymal semen and ejaculated semen is crucial for optimizing cryopreservation and enhancing the applications of assisted reproductive technologies. Factors affecting epididymal semen quality and freezability need to be identified and addressed to improve post-thaw semen quality and ART outcomes.
Article
Microbiology
Aleksandar Cojkic, Adnan Niazi, Yongzhi Guo, Triin Hallap, Peeter Padrik, Jane M. Morrell
Summary: This study identified bacteria in the semen of healthy bulls using 16S sequencing, revealing differences in the microbiota between individual bulls that may be correlated with fertility. The presence of certain bacterial genera was negatively associated with bull fertility, while two genera, W5053 and Lawsonella, were enriched in bulls of low fertility, marking the first reported detection of these bacteria in bull semen samples.
Article
Biochemistry & Molecular Biology
Eva Tvrda, Michal Duracka, Filip Benko, Anton Kovacik, Daniel Lovisek, Eliska Galova, Jana Ziarovska, Marianna Schwarzova, Miroslava Kacaniova
Summary: This study aimed to describe the bacterial profiles of ejaculates collected after different ejaculatory abstinence periods and explore the associated changes in conventional, oxidative, and immunological characteristics of semen. The analysis revealed that samples collected after two days of abstinence had higher bacterial load and diversity, as well as a greater prevalence of potentially uropathogenic bacteria. However, samples obtained after two hours of abstinence showed a lower occurrence of bacteria. Additionally, shorter ejaculatory abstinence was found to contribute to better sperm quality and reduced damage by reactive oxygen species and pro-inflammatory cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cinzia Signorini, Elena Moretti, Daria Noto, Lucia Micheli, Rosetta Ponchia, Giulia Collodel
Summary: Specialized pro-resolving lipid mediators, such as resolvin D1 (RvD1), play a role in regulating acute inflammation and resolving it. This study investigated the implication of seminal RvD1 in human infertility and found that higher levels of RvD1 were associated with increased inflammation markers and reduced sperm quality in infertile patients. The study suggests using a panel of inflammatory markers and lipid mediators for diagnosing and treating inflammatory conditions.
Article
Public, Environmental & Occupational Health
Sofia Passaponti, Cinzia Signorini, Giulia Collodel, Elena Moretti, Simona Mattioli, Giuseppe Belmonte, Daria Noto
Summary: This study investigated the expression and localization of PPAR gamma, as well as susceptibility to lipoperoxidation, in the reproductive tissues of rabbits fed n-3 polyunsaturated fatty acid (PUFA)-enriched diets. The results showed that PPAR gamma expression was down-regulated in control rabbit testes but up-regulated in the epididymis of rabbits fed n-3 PUFAs diets. The study suggests that n-3 PUFAs diets may influence PPAR gamma activity and play a role in supporting sperm maturation.
JOURNAL OF MENS HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Simona Mattioli, Corrado Dimauro, Alberto Cesarani, Alessandro Dal Bosco, Desiree Bartolini, Francesco Galli, Anna Migni, Bartolomeo Sebastiani, Cinzia Signorini, Camille Oger, Giulia Collodel, Cesare Castellini
Summary: The study aimed to develop a dynamic model using rabbits as a model to evaluate the balance between reactive oxygen species, polyunsaturated fatty acids, and antioxidants. The model showed good accuracy in predicting the effects of dietary treatments on the redox homeostasis of rabbits.
Review
Biochemistry & Molecular Biology
Giulia Collodel, Elena Moretti, Daria Noto, Roberta Corsaro, Cinzia Signorini
Summary: This review examines the role of fatty acids in human pathological conditions, particularly infertility. Fatty acids and their derivatives play important roles in regulating cell membrane composition, cell signaling, cell death, immune responses, and inflammation. Understanding the metabolism of fatty acids can lead to new approaches in treating infertility.
Article
Immunology
Elena Moretti, Cinzia Signorini, Daria Noto, Sergio Antonio Tripodi, Andrea Menchiari, Ester Sorrentino, Giulia Collodel
Summary: This study explored the relationships among omentin-1/ITLN1, semen parameters, and oxidative stress markers in different patient groups. The levels of omentin-1/ITLN1 were found to be correlated with F-2-IsoP levels and negatively correlated with sperm parameters. Infertile patients showed lower levels of omentin-1/ITLN1, while those with varicocele and genitourinary infections had higher levels. Omentin-1/ITLN1 was localized differently in sperm and male reproductive system tissues.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Giulia Collodel, Elena Moretti, Daria Noto, Roberta Corsaro, Cinzia Signorini, Claudia Bonechi, Lorenzo Cangeloni, Giovanni Luca, Iva Arato, Francesca Mancuso
Summary: This study aimed to investigate the effects of different types of liposomes on porcine prepubertal Sertoli cells (SCs) and to find a suitable carrier for in vitro testing of drugs. The results showed that anionic and cationic liposomes altered the distribution of actin and vimentin filaments and increased the phosphorylation levels of AMPK alpha. Zwitterionic liposomes, on the other hand, did not cause any changes in the cell cytoplasm. These preliminary data suggest that zwitterionic liposomes are the most suitable carrier for in vitro studies of SCs to understand the effects of molecules or drugs with potential clinical applications in the treatment of certain forms of male infertility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Giulia Collodel, Laura Gambera, Anita Stendardi, Fabiola Nerucci, Cinzia Signorini, Caterina Pisani, Marzia Marcheselli, Francesca Letizia Vellucci, Silvana Enrica Pizzasegale, Lucia Micheli, Elena Moretti
Summary: This study investigated the changes and relationships of various molecules in the follicular fluid (FF) of infertile women with different reproductive conditions. It found that some biochemical components, hormones, antioxidant enzymes, F-2-Isoprostanes (F-2-IsoPs), and resolvin (Rv) D1 in the FF are associated with follicular growth, oocyte maturation, and female infertility. These findings highlight the importance of personalized therapeutic approaches and optimized assisted reproductive techniques (ART) outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Elena Moretti, Cinzia Signorini, Roberta Corsaro, Maria Giamalidi, Giulia Collodel
Summary: Spermatozoa produce reactive oxygen species (ROS) due to aerobic metabolism, which can have both beneficial and detrimental effects on cell function. Assisted reproductive procedures, such as cryopreservation, can lead to excessive ROS production and oxidative damage to spermatozoa. This review focuses on using human sperm as an in vitro model to study the supplementation of antioxidants in media. The presence and synergic effects of different antioxidant molecules may lead to more effective products in vitro and potentially in vivo.
Article
Biochemistry & Molecular Biology
Simona Mattioli, Elena Moretti, Cesare Castellini, Cinzia Signorini, Roberta Corsaro, Elisa Angelucci, Giulia Collodel
Summary: This study investigated the association of apelin and resolvin (RvD1) in the testis and sperm with the oxidative status of semen and serum testosterone in rabbits fed different diets enriched with flaxseed or fish oil. The results showed that flaxseed diet increased the levels of apelin in the testis and enhanced serum testosterone. Both enriched diets showed higher levels of malondialdehyde and RvD1 in the testis. Apelin and its receptor were also detected in ejaculated sperm.
Article
Biochemistry & Molecular Biology
Elena Moretti, Cinzia Signorini, Daria Noto, Roberta Corsaro, Lucia Micheli, Thierry Durand, Camille Oger, Jean Marie Galano, Giulia Collodel
Summary: After incubation with F-4-NeuroPs, selected human sperm showed increased motility, membrane mitochondrial potential, and reacted acrosome percentage. Chromatin integrity did not differ between treated and control samples. PSA staining highlighted a significantly higher number of sperm with reacted acrosomes in the treated group after 4 hours. Immunofluorescence analysis revealed a dense distribution of ryanodine receptors in the midpiece and postacrosomal sheath of sperm after 2 hours, followed by a dotted distribution after 4 hours. F-4-NeuroPs at an appropriate concentration can induce sperm capacitation via ion channels, which may enhance the success of assisted fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
