Article
Biochemistry & Molecular Biology
Zhengyu Piao, Jin Kyung Park, So-Jung Park, Byeongmoon Jeong
Summary: A polypeptide-based thermogel has been developed for hypothermic storage of stem cells at ambient temperature, showing higher cell recovery rate and healthier stem cell status compared to traditional preservation solutions. Membrane stabilization is suggested as a cell protection mechanism in the cytocompatible thermogel, offering a promising tool for cell transportation and short-term banking.
Review
Medical Laboratory Technology
Emma S. Heaton, S. H. A. Jin
Summary: Scientists have developed the bi-hormonal abnormality hypothesis, stating that diabetes is not solely caused by impaired insulin signaling but also by inappropriate levels of glucagon. Traditional treatments for type 1 diabetes rely on insulin injections, disregarding the role of other endocrine cell types. Therefore, there is a need to develop pancreatic islet organoids that include all cell types to better replace the functionality of native islets.
TRANSLATIONAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Gabriela Antonio-Andres, Gustavo U. Martinez-Ruiz, Mario Morales-Martinez, Elva Jimenez-Hernandez, Estefany Martinez-Torres, Tania V. Lopez-Perez, Laura A. Estrada-Abreo, Genaro Patino-Lopez, Sergio Juarez-Mendez, Victor M. Davila-Borja, Sara Huerta-Yepez
Summary: This study reveals that HIF-1 alpha can transcriptionally regulate YY1, and the expression of HIF-1 alpha positively correlates with YY1 in leukemia patients, suggesting that they may serve as therapeutic targets or biomarkers for ALL.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Nasim Kheshtchin, Jamshid Hadjati
Summary: Hypoxia, a common characteristic of solid tumors, contributes to different aspects of tumor progression and limits the efficacy of immunotherapies. Developing new immunotherapy strategies involving therapeutic targeting of HIF-1 molecules associated with hypoxia may enhance the clinical effectiveness of immunotherapy. Targeting hypoxia presents a potential opportunity to improve the clinical benefit of cancer immunotherapy.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Fisheries
Ming Li, Giana Bastos Gomes, Weishan Zhao, Guangran Hu, Ke Huang, Tomoyoshi Yoshinaga, Theodore G. Clark, Wenxiang Li, Hong Zou, Shangong Wu, Guitang Wang
Summary: This review discusses the artificial culture methods of six important parasitic ciliates affecting the fish industry and describes the challenges and future applications of parasite culture.
REVIEWS IN AQUACULTURE
(2022)
Article
Biochemistry & Molecular Biology
Maciej Jaskiewicz, Adrianna Moszynska, Jaroslaw Kroliczewski, Aleksandra Cabaj, Sylwia Bartoszewska, Agata Charzynska, Magda Gebert, Michal Dabrowski, James F. Collawn, Rafal Bartoszewski
Summary: HIF is a transcription factor that activates the adaptive hypoxic response during low oxygen levels. HIF-1 and HIF-2 mediate this response in a sequential manner, but their transcriptional profiles differ and the transition between them is not well understood.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Alexander Pietras
Summary: A newfound deubiquitinase has been identified to differentially stabilize HIF-2alpha in stem-like glioblastoma cells, shedding light on the regulation of hypoxic response in various tissues and cancers.
Article
Chemistry, Multidisciplinary
Mengdong Cui, Linfeng Liu, Liang Chen, Hengxin Han, Taijie Zhan, Hangyu Dang, Guoliang Yang, Yi Xu
Summary: Droplet-based vitrification is a promising cryopreservation method that achieves high cell viability through high cooling rates and low concentrations of cryoprotective agents. This study developed a chip with a high cooling rate for droplet encapsulation and provided a new method for continuous loading of low-concentration cryoprotective agent droplets. A theoretical model was also proposed to predict the cryoprotective agent concentration inside the microdroplets based on evaporation time. This research provides a potential method for low-concentration cryoprotective agent loading in cell vitrification preservation.
Review
Obstetrics & Gynecology
Brid Ni Dhonnabhain, Nagla Elfaki, Kyra Fraser, Aviva Petrie, Benjamin P. Jones, Srdjan Saso, Paul J. Hardiman, Natalie Getreu
Summary: This study compares obstetric outcomes in cancer patients who undergo cryopreservation of reproductive cells or tissues before gonadotoxic therapy. The results indicate that cryopreservation of oocytes, embryos, and ovarian tissue has similar effectiveness in terms of clinical pregnancy and live birth rates. However, ovarian tissue cryopreservation is associated with a significantly lower miscarriage rate compared to embryo cryopreservation.
FERTILITY AND STERILITY
(2022)
Review
Endocrinology & Metabolism
Lin Chen, Zirui Dong, Xiaoyan Chen
Summary: Survival rates for children and adolescents with malignancy have increased due to advances in oncology treatments, but these treatments can have toxic effects on the reproductive organs. Cryopreservation of oocytes and sperm is recognized as an effective strategy for preserving fertility in pubertal patients, while the use of hormonal agonists for ovarian protection is controversial. Ovarian tissue cryopreservation is the only option for prepubertal girls, but the outcomes after transplantation are varied. Immature testicular tissue cryopreservation is experimental for prepubertal boys. Guidelines for fertility preservation exist, but implementation in clinical practice is limited. This review aims to discuss indications, outcomes, and workflow for fertility preservation.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Adrianna Moszynska, Maciej Jaskiewicz, Marcin Serocki, Aleksandra Cabaj, David K. Crossman, Sylwia Bartoszewska, Magdalena Gebert, Michal Dabrowski, James F. Collawn, Rafal Bartoszewski
Summary: The cellular adaptive response to hypoxia is regulated by hypoxia-inducible factors (HIFs), HIF-1 and HIF-2. miRNAs play a significant role in modulating cellular signaling pathways during the hypoxic adaptive response.
Article
Medicine, Research & Experimental
Asli Okan, Zuleyha Doganyigit, Ece Eroglu, Enes Akyuz, Necdet Demir
Summary: The study aimed to evaluate the immunoexpression of adaptive responses to hypoxia in pancreatic and cardiac tissues of type 1 DM mice. It was found that TNF-alpha, HIF-1 alpha, and Kir6.2 immunoreactivity significantly increased in both tissues, suggesting potential roles of these factors in the mechanism of type 1 DM.
Article
Multidisciplinary Sciences
Martha Campbell-Thompson, Elizabeth A. Butterworth, J. Lucas Boatwright, Malavika A. Nair, Lith H. Nasif, Kamal Nasif, Andy Y. Revell, Alberto Riva, Clayton E. Mathews, Ivan C. Gerling, Desmond A. Schatz, Mark A. Atkinson
Summary: The dysregulation of glucagon secretion in type 1 diabetes involves the sympathetic nervous system and noradrenalin degradation pathways. The study found that sympathetic innervation at islets and intrinsic adrenergic signaling pathways could be novel targets for improving glucagon secretion in T1D patients.
SCIENTIFIC REPORTS
(2021)
Review
Gastroenterology & Hepatology
Zi-Niu Ding, Zhao-Ru Dong, Zhi-Qiang Chen, Ya-Fei Yang, Lun-Jie Yan, Hai-Chao Li, Kai-Xuan Liu, Cheng-Yu Yao, Yu-Chuan Yan, Chun-Cheng Yang, Tao Li
Summary: The study revealed that overexpression of HIF-1 alpha in hepatocellular carcinoma is associated with poorer prognosis and clinicopathological features, while HIF-2 alpha overexpression does not show the same associations. This suggests different roles of HIF-1 alpha and HIF-2 alpha in the progression and prognosis of hepatocellular carcinoma.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Cell & Tissue Engineering
Toshiharu Yamashita, Cat-Khanh Vuong, Nhat-Hoang Ngo, Motoo Osaka, Yuji Hiramatsu, Osamu Ohneda
Summary: Long-term glucocorticoid treatment impairs wound healing ability in adipose tissue-derived mesenchymal stem cells (sAT-MSC) due to downregulation of SDF-1. Our study found that sAT-MSC showed impaired HIF-1a and upregulated HIF-2a levels, with HIF-2a inhibition leading to compensatory overexpression of HIF-1a and SDF-1, improving wound healing ability. Additionally, HIF-2a reduction promoted wound healing effects and increased inflammatory cell recruitment through HIF-1a/SDF-1 axis.
STEM CELLS AND DEVELOPMENT
(2023)