Effect of treatment temperature on collagen structures of the decellularized carotid artery using high hydrostatic pressure

Decellularized tissues have attracted a great deal of attention as regenerating transplantation materials. A decellularizing method based on high hydrostatic pressure (HHP) has been developed, and the preparation of many types of decellularized tissues has been investigated, including aorta, cornea, and dermis. The preparation of a small-diameter vascular graft was studied using a carotid artery from the viewpoint of collagen denaturation and leakage. After HHP, the carotid artery was washed at two washing temperatures (37 and 4A degrees C). Histological evaluation, collagen content measurement and circular dichroism (CD) measurement indicated that the washing temperatures clearly affected the collagen structure of the decellularized carotid artery. The amount of collagen decreased in the carotid artery decellularized by HHP washed at 37A degrees C (HHP/37A degrees C). On the other hand, the amount and structure of collagen were preserved in the carotid artery washed at 4A degrees C after HHP (HHP/4A degrees C). In rat carotid artery syngeneic transplantation, the HHP/37A degrees C decellularized carotid artery occluded after 2 weeks, but the HHP/4A degrees C decellularized one did not. These results indicate that collagen denaturation and leakage of the decellularized carotid artery affect the in vivo performance of the carotid artery.