Article
Engineering, Biomedical
Tomoyuki Kojima, Takashi Nakamura, Junichi Saito, Yuko Hidaka, Taisuke Akimoto, Hana Inoue, Christian Nanga Chick, Toyonobu Usuki, Makoto Kaneko, Etsuko Miyagi, Yoshihiro Ishikawa, Utako Yokoyama
Summary: Periodic hydrostatic pressurization under hypoxia enables the fabrication of scaffoldless human vascular mimetics with a spatial arrangement of cells and matrices. These mimetics show excellent mechanical properties and biocompatibility, making them suitable for clinical applications in cardiovascular diseases.
ACTA BIOMATERIALIA
(2023)
Article
Chemistry, Physical
Xiaoqian Ge, Xueqi Xu, Qi Cai, Hejian Xiong, Chen Xie, Yi Hong, Xiaofei Gao, Yao Yao, Robert Bachoo, Zhenpeng Qin
Summary: The pan-ECM method enables live imaging of the entire brain extracellular matrix, providing insights into its heterogeneity and changes in neurological disorders. This novel approach has the potential to advance our understanding of brain function and neurological diseases.
Article
Engineering, Biomedical
Jie Shi, Yanjiao Teng, Duo Li, Ju He, Adam C. Midgley, Xiaoqin Guo, Xiudan Wang, Xinran Yang, Shufang Wang, Yakai Feng, Qi Lv, Shike Hou
Summary: In this study, a tri-layered vascular graft was constructed using decellularized extracellular matrix (dECM) to mimic the defining features of native arteries. The results showed that dECM provided a bioactive environment for endothelial cell growth and migration. Additionally, it promoted smooth muscle regeneration and extracellular matrix deposition. This study provides a promising strategy for small-diameter vascular grafts.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Won-Woo Cho, Byoung Soo Kim, Minjun Ahn, Yeon Hee Ryu, Dong-Heon Ha, Jeong Sik Kong, Jong-Won Rhie, Dong-Woo Cho
Summary: This study introduces a new concept of assembling cell-laden tissue modules for soft tissue engineering, aiming to achieve nonhypoxic, flexible, and volume-stable tissue assembly. The significance of engineered tissue assembly is demonstrated through various evaluations, showing stable volume and significant neovascularization/adipogenesis in the implanted assembly. The work presents a novel approach of cell printing-based tissue assembly for functional reconstruction of soft tissue.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Cardiac & Cardiovascular Systems
Ana Rubina Perestrelo, Ana Catarina Silva, Jorge Oliver-De La Cruz, Fabiana Martino, Vladimir Horvath, Guido Caluori, Ondrej Polansky, Vladimir Vinarsky, Giulia Azzato, Giuseppe de Marco, Vita Zampachova, Petr Skladal, Stefania Pagliari, Alberto Rainer, Perpetua Pinto-do-O, Alessio Caravella, Kamila Koci, Diana S. Nascimento, Giancarlo Forte
Summary: The study reveals that the remodeling of cardiac extracellular matrix (ECM) during heart failure (HF) leads to cardiac fibroblast activation and focal adhesion protein expression through hyperactivated YAP signaling, impacting cell homing.
CIRCULATION RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Francisco Ramirez-Perez, Francisco J. Cabral-Amador, Adam T. Whaley-Connell, Annayya R. Aroor, Mariana Morales-Quinones, Makenzie L. Woodford, Thaysa Ghiarone, Larissa Ferreira-Santos, Thomas J. Jurrissen, Camila M. Manrique-Acevedo, GuangHong Jia, Vincent G. DeMarco, Jaume Padilla, Luis A. Martinez-Lemus, Guido Lastra
Summary: Consumption of diets high in fat, sugar, and salt is associated with arterial stiffening, which is a risk factor for cardiovascular disease. This study demonstrates that using the TG2 inhibitor cystamine can reduce arterial stiffness in female mice fed a Western diet. This is likely due to cystamine's ability to reduce TG2 activity and improve the structure of the blood vessel walls.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hyeonyu Kim, Tatsuya Osaki, Roger D. Kamm, H. Harry Asada
Summary: This study develops an engineered muscle tissue with a coaxial three-layered tubular structure that resembles the architecture of native skeletal muscles. By remodeling the extracellular matrix, the engineered skeletal muscle tissues produced stronger contractile force and improved angiogenesis.
Review
Biotechnology & Applied Microbiology
Victor Alfonso Solarte David, Viviana Raquel Guiza-Argueello, Martha L. Arango-Rodriguez, Claudia L. Sossa, Silvia M. Becerra-Bayona
Summary: The absence or damage of tissue is a major cause of diseases, and novel therapeutic alternatives aim to recover lost functions through tissue regeneration. Chronic cutaneous lesions are common wounds that require regenerative medicine and tissue engineering interventions to develop bioactive medical products for proper healing and prevention of complications. The coordination of cells, extracellular matrix (ECM), and biomolecules is crucial in tissue repair and regeneration, with ECM acting as a biological platform for their interactions. Tissue engineering utilizes synthetic polymers and decellularized tissues to mimic native ECM and provide necessary functionalities for cell differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Emna Ouni, Alexis Peaucelle, Kalina T. Haas, Olivier Van Kerk, Marie-Madeleine Dolmans, Timo Tuuri, Marjut Otala, Christiani A. Amorim
Summary: Despite the long history of human ovary dissection, the biophysical characteristics of ovarian tissue remain poorly understood. This study reveals a unique biophysical phenotype of reproductive-age tissue, providing insights for designing artificial ovaries and potential applicability to characterizing other organs in physiological or pathological states.
NATURE COMMUNICATIONS
(2021)
Article
Biophysics
Yuting Lou, Takumi Kawaue, Ivan Yow, Yusuke Toyama, Jacques Prost, Tetsuya Hiraiwa
Summary: This study elaborates on the force dynamics in a multilayer system with interfacial friction between a cell layer and an elastic substrate. By establishing an analytical model, it is discovered that sliding between substrate layers can result in force propagation, with the speed of propagation depending on the stiffness, thickness, and slipperiness of the substrate. These findings shed new light on force propagation in tissues.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Cell Biology
Meredith Whitehead, Syabira Yusoff, Sadia Ahmad, Lukas Schmidt, Manuel Mayr, Jillian Madine, David Middleton, Catherine M. Shanahan
Summary: Vascular amyloidosis, caused by the aggregation of peptide monomers into insoluble amyloid, is a common age-related pathology. This study found that extracellular vesicles (EVs) and HSPG2 in the extracellular matrix (ECM) play key roles in medin accumulation, contributing to the development of age-associated aortic medial amyloid (AMA).
Review
Biotechnology & Applied Microbiology
Danielle Yarbrough, Sharon Gerecht
Summary: Vascular smooth muscle cells play a vital role in regulating blood pressure and maintaining cardiovascular health. Changes in their surrounding environment can lead to alterations in phenotype and secretory profile, causing pathological conditions. Understanding the interaction between smooth muscle cells and the extracellular matrix is crucial for treating vascular diseases.
BIOENGINEERING-BASEL
(2022)
Article
Materials Science, Biomaterials
Chengzhao Tu, Yuanguo Zhang, Yonghao Xiao, Yuehao Xing, Yuhao Jiao, Xue Geng, Aiying Zhang, Lin Ye, Yongquan Gu, Zengguo Feng
Summary: Tissue engineering is a promising strategy to develop successful small diameter vascular grafts (SDVG). This study focused on optimizing the fabrication of SDVGs by introducing natural substances into synthetic biomaterials, improving biocompatibility and promoting in vivo regeneration. Bioactive SDVGs were prepared by coating heparin-modified poly-(epsilon-caprolactone) scaffolds with vascular extracellular matrix (VECM) components. The SDVGs showed good endothelial cell proliferation and in vivo regeneration, indicating the specificity and effectiveness of the fabricated VECM-SDVGs.
BIOMATERIALS ADVANCES
(2022)
Article
Multidisciplinary Sciences
T. Mizuno, E. Hase, T. Minamikawa, Y. Tokizane, R. Oe, H. Koresawa, H. Yamamoto, T. Yasui
Summary: This study demonstrates scan-less full-field FLIM based on frequency-multiplexed RF signals, allowing for rapid acquisition of bimodal images of fluorescence amplitude and lifetime without the need for mechanical scanning. This technique has the potential to be useful for quantitative fluorescence imaging in life science.
Article
Engineering, Biomedical
Tomoko G. Oyama, Kotaro Oyama, Atsushi Kimura, Fumiya Yoshida, Ryo Ishida, Masashi Yamazaki, Hiromi Miyoshi, Mitsumasa Taguchi
Summary: This study introduces novel collagen hydrogels that can replicate the correlation between collagen concentration and elastic modulus in native ECM, introducing soft topographic cues to cell culture. These collagen hydrogels can influence cell morphology and function, reducing disparities in cell behavior and allowing for controlled cell manipulation for tissue engineering applications.
BIOMEDICAL MATERIALS
(2021)
Article
Biophysics
Keiichi Takamizawa
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
(2015)
Article
Engineering, Biomedical
Yasuhide Nakayama, Kanna Okuda, Keiichi Takamizawa, Atsuyoshi Nakayama
ACTA BIOMATERIALIA
(2011)
Article
Engineering, Biomedical
Hiroyuki Kadono, Tsutomu Furuzono, Miwa Masuda, Masahiro Okada, Mitsuki Ueki, Keiichi Takamizawa, Ryoichi Tanaka, Kunio Miyatake, Yoshihisa Koyama, Kazuo Takakuda
Article
Mechanics
Keiichi Takamizawa, Yasuhide Nakayama
JOURNAL OF APPLIED MECHANICS-TRANSACTIONS OF THE ASME
(2013)
Article
Engineering, Biomedical
Takeshi Moriwaki, Tomonori Oie, Keiichi Takamizawa, Yoshinobu Murayama, Toru Fukuda, Sadao Omata, Keiichi Kanda, Yasuhide Nakayama
JOURNAL OF ARTIFICIAL ORGANS
(2011)
Article
Engineering, Biomedical
Takeshi Moriwaki, Tomonori Oie, Keiichi Takamizawa, Yoshinobu Murayama, Toru Fukuda, Sadao Omata, Yasuhide Nakayama
JOURNAL OF ARTIFICIAL ORGANS
(2013)
Article
Biophysics
Keiichi Takamizawa, Yasuhide Nakayama
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
(2013)
Article
Engineering, Biomedical
Osamu Sakai, Keiichi Kanda, Keiichi Takamizawa, Takayuki Sato, Hitoshi Yaku, Yasuhide Nakayama
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2009)
Article
Engineering, Biomedical
Nobuhiro Nagai, Yasuhide Nakayama, Yue-Min Zhou, Keiichi Takamizawa, Kazuo Mori, Masanobu Munekata
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2008)
Article
Engineering, Biomedical
Haiying Huang, Yue-Min Zhou, Hatsue Ishibashi-Ueda, Keiichi Takamizawa, Joji Ando, Keiichi Kanda, Hitoshi Yaku, Yasuhide Nakayama
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2009)
Article
Engineering, Biomedical
Yasuhide Nakayama, Saori Yamaoka, Masashi Yamanami, Megumi Fujiwara, Masami Uechi, Keiichi Takamizawa, Hatsue Ishibashi-Ueda, Marie Nakamichi, Kingo Uchida, Taiji Watanabe, Keiichi Kanda, Hitoshi Yaku
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2011)
Article
Cardiac & Cardiovascular Systems
Keiichi Takamizawa
CARDIOVASCULAR ENGINEERING AND TECHNOLOGY
(2019)
Article
Biophysics
Keiichi Takamizawa
Summary: The study aims to understand stress distributions in arterial walls under residual stresses using a two-layer model. Analysis on human common carotid arteries under physiological and supraphysiological intraluminal pressures revealed that stress increased from the inner surface to the outer surface.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2022)
Article
Engineering, Biomedical
Osamu Sakai, Keiichi Kanda, Hatsue Ishibashi-Ueda, Keiichi Takamizawa, Akihiro Ametani, Hitoshi Yaku, Yasuhide Nakayama
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2007)